Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,016 A→C 6.9% D69E (GAT→GAG glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0160AC 6.9% 415.3 / 20.2 116D69E (GAT→GAGglpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (53/55);  new base C (4/4);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 116 total aligned reads displayed.

AATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAT  >  NC_000913/4116877‑4117159
                                                                                                                                           |                                                                                                                                               
aaTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTc                                                                                                                                        <  4:206212/149‑1 (MQ=255)
aaTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTc                                                                                                                                        <  2:1140389/149‑1 (MQ=255)
     tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAg                                                                                                                                   >  3:565603/1‑149 (MQ=255)
      cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGc                                                                                                                                  >  3:2403/1‑149 (MQ=255)
       gCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCa                                                                                                                                 >  3:1171547/1‑149 (MQ=255)
       gCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCa                                                                                                                                 >  1:717489/1‑149 (MQ=255)
             ttCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTa                                                                                                                           <  2:924158/149‑1 (MQ=255)
               cTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                                                          >  3:1193300/1‑148 (MQ=255)
               cTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                                                          <  4:1193300/148‑1 (MQ=255)
                      acgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTgg                                                                                                                  <  4:1171547/149‑1 (MQ=255)
                       cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGGGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGTACTTCTACCAGCGTGGa                                                                                                                 >  1:1106317/1‑149 (MQ=255)
                         acgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                                                          >  2:194347/1‑138 (MQ=255)
                         acgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                                                          <  1:194347/138‑1 (MQ=255)
                              aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAg                                                                                                                                   <  2:286489/124‑1 (MQ=255)
                                tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCt                                                                                                                            <  2:754980/129‑1 (MQ=255)
                                 gCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTg                                                                                                       >  3:1342391/1‑149 (MQ=255)
                                        aaTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                                                                <  1:673782/149‑1 (MQ=255)
                                         aTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                               >  1:242404/1‑149 (MQ=255)
                                             cGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                                                           <  4:1342391/149‑1 (MQ=255)
                                                   agatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                     >  4:544436/1‑149 (MQ=255)
                                                     atagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                    >  2:1300445/1‑116 (MQ=255)
                                                      tagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                                          >  4:267397/1‑125 (MQ=255)
                                                      tagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCg                                                                                  >  2:1060059/1‑149 (MQ=255)
                                                         gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                               >  3:184579/1‑149 (MQ=255)
                                                           ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGcc                                                                                                                                     >  1:1357098/1‑93 (MQ=255)
                                                           ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGcc                                                                                                                                     <  2:1357098/93‑1 (MQ=255)
                                                           ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                                                                <  4:585695/130‑1 (MQ=255)
                                                           ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                                                                >  3:585695/1‑130 (MQ=255)
                                                           ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                               <  3:1344013/147‑1 (MQ=255)
                                                           ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                               >  4:1344013/1‑147 (MQ=255)
                                                            tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                            <  2:717489/149‑1 (MQ=255)
                                                               cGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                         >  3:1425790/1‑149 (MQ=255)
                                                                ggTTTCTTTTTCCCGGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAccc                                                                        >  3:107393/1‑149 (MQ=255)
                                                                  tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTc                                                                                                                             <  2:634291/94‑1 (MQ=255)
                                                                  tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTc                                                                                                                             >  1:634291/1‑94 (MQ=255)
                                                                  tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCt                                                                                                                            >  3:1297087/1‑95 (MQ=255)
                                                                  tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCt                                                                                                                            <  4:1297087/95‑1 (MQ=255)
                                                                  tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                      >  4:781135/1‑149 (MQ=255)
                                                                   ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTTCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                   <  4:853803/135‑1 (MQ=255)
                                                                   ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                   >  3:853803/1‑135 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                                          <  1:1000280/109‑1 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                                          >  2:1000280/1‑109 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTg                                                                                       >  1:795801/1‑128 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                               >  3:388558/1‑136 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                               <  4:388558/136‑1 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                     >  1:1235231/1‑146 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                     <  2:1235231/146‑1 (MQ=255)
                                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                  >  3:1363353/1‑149 (MQ=255)
                                                                        tttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                                                                <  3:544436/149‑1 (MQ=255)
                                                                           cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                             <  3:781135/149‑1 (MQ=255)
                                                                            ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                    >  3:908698/1‑77 (MQ=255)
                                                                             cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  2:242404/149‑1 (MQ=255)
                                                                             cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  1:765591/149‑1 (MQ=255)
                                                                             cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  1:1060059/149‑1 (MQ=255)
                                                                               gACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGt                                                         >  2:1248445/1‑149 (MQ=255)
                                                                               gACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGt                                                         >  2:784412/1‑149 (MQ=255)
                                                                                 cAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                                                       <  4:1425790/149‑1 (MQ=255)
                                                                                         gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTg                                                                                                       <  2:11468/93‑1 (MQ=255)
                                                                                         gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTg                                                                                                       >  1:11468/1‑93 (MQ=255)
                                                                                         gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  1:1460077/137‑1 (MQ=255)
                                                                                         gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           >  2:1460077/1‑137 (MQ=255)
                                                                                          tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                                                   <  4:1086732/128‑1 (MQ=255)
                                                                                          tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                                                   >  3:1086732/1‑128 (MQ=255)
                                                                                             cACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  4:1096318/133‑1 (MQ=255)
                                                                                                 cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGc                                                                                                                                      <  4:789267/54‑1 (MQ=255)
                                                                                                 cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGc                                                                                                                                      >  3:789267/1‑54 (MQ=255)
                                                                                                 cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                <  3:162001/108‑1 (MQ=255)
                                                                                                 cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                >  4:162001/1‑108 (MQ=255)
                                                                                                 cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                                                                <  1:328570/124‑1 (MQ=255)
                                                                                                   ggTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCgcgc                                     <  2:1106317/149‑1 (MQ=255)
                                                                                                    gTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  3:313862/126‑1 (MQ=255)
                                                                                                    gTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           >  4:313862/1‑126 (MQ=255)
                                                                                                    gTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                               <  1:1071817/90‑1 (MQ=255)
                                                                                                     ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGttt                                                              <  4:1462352/122‑1 (MQ=255)
                                                                                                     ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGttt                                                              >  3:1462352/1‑122 (MQ=255)
                                                                                                         tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                               >  3:260423/1‑149 (MQ=255)
                                                                                                            tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                            >  3:441389/1‑149 (MQ=255)
                                                                                                               cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                         >  3:1050623/1‑101 (MQ=255)
                                                                                                               cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                         >  1:281568/1‑101 (MQ=255)
                                                                                                               cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                         <  4:1050623/101‑1 (MQ=255)
                                                                                                               cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                         <  2:281568/101‑1 (MQ=255)
                                                                                                                 aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                        <  4:739466/148‑1 (MQ=255)
                                                                                                                 aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                        >  3:739466/1‑148 (MQ=255)
                                                                                                                 aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                       <  4:441389/149‑1 (MQ=255)
                                                                                                                     cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata                   <  4:260423/149‑1 (MQ=255)
                                                                                                                      tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                  >  1:1197609/1‑149 (MQ=255)
                                                                                                                      tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                  >  1:895151/1‑149 (MQ=255)
                                                                                                                              gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                             >  2:905211/1‑98 (MQ=255)
                                                                                                                              gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                             <  1:905211/98‑1 (MQ=255)
                                                                                                                               aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                               <  4:657426/79‑1 (MQ=255)
                                                                                                                                tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           >  2:648603/1‑98 (MQ=255)
                                                                                                                                tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                           <  1:648603/98‑1 (MQ=255)
                                                                                                                                tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTc                                         <  1:492654/116‑1 (MQ=255)
                                                                                                                                tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTc                                         >  2:492654/1‑116 (MQ=255)
                                                                                                                                 cGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa       <  4:184579/149‑1 (MQ=255)
                                                                                                                                 cGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa       <  2:895151/149‑1 (MQ=255)
                                                                                                                                   gAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                           <  3:202267/111‑1 (MQ=255)
                                                                                                                                   gAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                           >  4:202267/1‑111 (MQ=255)
                                                                                                                                      cTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAt  <  2:1197609/149‑1 (MQ=255)
                                                                                                                                       tGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                                              <  1:1322895/72‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
AATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAT  >  NC_000913/4116877‑4117159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: