Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,174 A→C 93.1% S17A (TCC→GCC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,1740AC93.1% 413.1 / 15.2 116S17A (TCC→GCC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base C (53/55);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 116 total aligned reads displayed.

ACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT  >  NC_000913/4117038‑4117318
                                                                                                                                        |                                                                                                                                                
aCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGcc                                                                                                                                      <  4:1443563/149‑1 (MQ=255)
    gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTg                                                                                                                                   >  2:1437594/1‑148 (MQ=255)
    gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTg                                                                                                                                   <  1:1437594/148‑1 (MQ=255)
         gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAg                                                                                                                             >  3:670664/1‑149 (MQ=255)
             tttGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCtggt                                                                                                                                            >  2:741185/1‑130 (MQ=255)
             tttGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCtggt                                                                                                                                            <  1:741185/130‑1 (MQ=255)
                 ggTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGa                                                                                                                     >  3:34425/1‑149 (MQ=255)
                       ccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCt                                                                                                                                               >  4:536565/1‑117 (MQ=255)
                       ccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCt                                                                                                                                               <  3:536565/117‑1 (MQ=255)
                       ccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt                                                                                                               >  1:255396/1‑149 (MQ=255)
                       ccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt                                                                                                               >  2:830494/1‑149 (MQ=255)
                       ccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt                                                                                                               >  3:809958/1‑149 (MQ=255)
                           aTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                                                                                           >  1:752286/1‑149 (MQ=255)
                                cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGatata                                                                                                                 <  1:1446613/138‑1 (MQ=255)
                                cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGatata                                                                                                                 >  2:1446613/1‑138 (MQ=255)
                                    gggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAt                                                                                                  <  1:1248445/149‑1 (MQ=255)
                                      gTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGt                                                                                                <  2:752286/149‑1 (MQ=255)
                                            ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCtggtg                                                                                                                                           >  3:1192427/1‑100 (MQ=255)
                                            ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCtggtg                                                                                                                                           <  4:1192427/100‑1 (MQ=255)
                                            ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGa                                                                                                                              >  4:898434/1‑113 (MQ=255)
                                            ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                                                                                          >  3:196507/1‑149 (MQ=255)
                                            ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                                                                                          >  2:271913/1‑149 (MQ=255)
                                            ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGCGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGa                                                                                                                              <  3:898434/113‑1 (MQ=255)
                                               tACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTc                                                                                       <  4:809958/149‑1 (MQ=255)
                                                  ccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                                                                    >  3:1237390/1‑149 (MQ=255)
                                                    aaCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTa                                                                                  >  1:377146/1‑149 (MQ=255)
                                                     aCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                 >  1:822386/1‑149 (MQ=255)
                                                       cTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGtt                                                                                               >  1:988787/1‑133 (MQ=255)
                                                       cTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGtt                                                                                               <  2:988787/133‑1 (MQ=255)
                                                       cTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTc                                                                               <  4:670664/149‑1 (MQ=255)
                                                           ttttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCa                                                                                                       >  3:1193101/1‑121 (MQ=255)
                                                           ttttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCa                                                                                                       <  4:1193101/121‑1 (MQ=255)
                                                           ttttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCatat                                                                           <  4:1237390/149‑1 (MQ=255)
                                                           ttttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCatat                                                                           >  2:662678/1‑149 (MQ=255)
                                                                ggTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAg                                                                      <  2:255396/149‑1 (MQ=255)
                                                                ggTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAg                                                                      <  2:47546/149‑1 (MQ=255)
                                                                   aGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCtggtg                                                                                                                                           >  3:524356/1‑77 (MQ=255)
                                                                   aGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCtggtg                                                                                                                                           <  4:524356/77‑1 (MQ=255)
                                                                     aTTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttttt                                                                                                            <  4:645345/106‑1 (MQ=255)
                                                                     aTTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAg                                                                 >  4:216353/1‑149 (MQ=255)
                                                                      tttGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc                                                                >  3:502234/1‑149 (MQ=255)
                                                                         gCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc                                                                                     >  3:275932/1‑125 (MQ=255)
                                                                         gCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc                                                                                     <  4:275932/125‑1 (MQ=255)
                                                                         gCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt                                                             <  2:377146/149‑1 (MQ=255)
                                                                         gCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt                                                             <  1:662678/149‑1 (MQ=255)
                                                                          cTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGa                                                                                                                              >  1:1000867/1‑83 (MQ=255)
                                                                          cTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGa                                                                                                                              <  2:1000867/83‑1 (MQ=255)
                                                                          cTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                                                                                           >  2:319678/1‑102 (MQ=255)
                                                                          cTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                                                                                           <  1:319678/102‑1 (MQ=255)
                                                                          cTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTTCCGTAGTCATATTACAGCGAAGCtttt                                                            >  1:794788/1‑149 (MQ=255)
                                                                           tCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGa                                                                                                                              >  2:870023/1‑82 (MQ=255)
                                                                           tCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGa                                                                                                                              <  1:870023/82‑1 (MQ=255)
                                                                           tCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGat                                                                                                                    <  2:981762/92‑1 (MQ=255)
                                                                           tCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttttt                                                           <  3:216353/149‑1 (MQ=255)
                                                                              aaTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttttt                                                           <  1:737961/146‑1 (MQ=255)
                                                                              aaTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttttt                                                           >  2:737961/1‑146 (MQ=255)
                                                                               aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTc                                                       <  4:196507/149‑1 (MQ=255)
                                                                                ttCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTc                                                                                                        <  4:421562/99‑1 (MQ=255)
                                                                                ttCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTc                                                                                                        >  3:421562/1‑99 (MQ=255)
                                                                                ttCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCtt                                                              >  1:783383/1‑141 (MQ=255)
                                                                                ttCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCtt                                                              <  2:783383/141‑1 (MQ=255)
                                                                                    cgcTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTTCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg                                                  <  2:794788/149‑1 (MQ=255)
                                                                                       tGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCt                                                                                                                                    >  3:960612/1‑64 (MQ=255)
                                                                                       tGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCt                                                                                                                                    <  4:960612/64‑1 (MQ=255)
                                                                                         cGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGtt                                             <  4:502234/149‑1 (MQ=255)
                                                                                           acacGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTtgt                                           <  1:271913/149‑1 (MQ=255)
                                                                                           acacGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                            >  4:479555/1‑100 (MQ=255)
                                                                                           acacGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                            <  3:479555/100‑1 (MQ=255)
                                                                                             acGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTcc                                                                                      <  2:902239/104‑1 (MQ=255)
                                                                                             acGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTcc                                                                                      >  1:902239/1‑104 (MQ=255)
                                                                                               gCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGtt                                       >  1:1360914/1‑149 (MQ=255)
                                                                                                 tAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTc                                     <  1:830494/149‑1 (MQ=255)
                                                                                                 tAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTc                                     <  2:190235/149‑1 (MQ=255)
                                                                                                 tAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTc                                     >  1:190235/1‑149 (MQ=255)
                                                                                                 tAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTc                                     <  2:1360914/149‑1 (MQ=255)
                                                                                                  aaTGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCtttt                                                            >  1:509726/1‑125 (MQ=255)
                                                                                                   aTGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACa                                                                       >  3:1239694/1‑113 (MQ=255)
                                                                                                   aTGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACa                                                                       <  4:1239694/113‑1 (MQ=255)
                                                                                                   aTGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttttt                                                           >  4:384191/1‑125 (MQ=255)
                                                                                                       tatTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc                                                                     <  2:1171523/111‑1 (MQ=255)
                                                                                                       tatTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc                                                                     >  1:1171523/1‑111 (MQ=255)
                                                                                                          tGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCata                                                                            >  3:1286191/1‑101 (MQ=255)
                                                                                                          tGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCata                                                                            <  4:1286191/101‑1 (MQ=255)
                                                                                                            gCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg                                                  >  3:189409/1‑125 (MQ=255)
                                                                                                            gCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg                                                  <  4:189409/125‑1 (MQ=255)
                                                                                                             cATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGtt                                                        <  3:1362997/118‑1 (MQ=255)
                                                                                                             cATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGtt                                                        >  4:1362997/1‑118 (MQ=255)
                                                                                                               tCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                                                                                          <  3:537341/82‑1 (MQ=255)
                                                                                                                  tGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGa                                                >  1:1134136/1‑121 (MQ=255)
                                                                                                                  tGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGa                                                <  2:1134136/121‑1 (MQ=255)
                                                                                                                     tCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGa                 >  3:975831/1‑149 (MQ=255)
                                                                                                                      ccATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGat                >  3:1182713/1‑149 (MQ=255)
                                                                                                                             gACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAt                                                                                                  <  4:106426/60‑1 (MQ=255)
                                                                                                                             gACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAt                                                                                                  >  3:106426/1‑60 (MQ=255)
                                                                                                                             gACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAt                                                                                                  <  2:856824/60‑1 (MQ=255)
                                                                                                                             gACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCAt                                                                                                  >  1:856824/1‑60 (MQ=255)
                                                                                                                             gACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCaa         <  4:1182713/149‑1 (MQ=255)
                                                                                                                             gACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCaa         >  1:417879/1‑149 (MQ=255)
                                                                                                                                 gcgcgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTg                                                     >  3:828685/1‑101 (MQ=255)
                                                                                                                                 gcgcgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTg                                                     <  4:828685/101‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                
ACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT  >  NC_000913/4117038‑4117318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: