| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,225,316 | A→C | 5.5% | K497Q (AAA→CAA) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,225,316 | 0 | A | C | 5.5% | 608.9 / 21.4 | 165 | K497Q (AAA→CAA) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (73/83); new base C (4/5); total (77/88) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
ATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGC > NC_000913/4225169‑4225461 | aTCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGaa > 3:848180/1‑149 (MQ=255) tCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGT‑ATTGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGaaaa > 1:1441270/1‑149 (MQ=255) ccAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGaaaa < 1:661469/149‑1 (MQ=255) cAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGCTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAAt < 4:1043952/149‑1 (MQ=255) ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCga < 4:848180/149‑1 (MQ=255) aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGAt > 3:208690/1‑149 (MQ=255) aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGAt > 4:710349/1‑149 (MQ=255) tGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTc < 1:440970/149‑1 (MQ=255) gTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCAATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGT‑ATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg < 2:1441270/149‑1 (MQ=255) aaCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:996191/149‑1 (MQ=255) aaCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 4:208690/149‑1 (MQ=255) tctATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGc < 1:88847/138‑1 (MQ=255) tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa > 2:879446/1‑149 (MQ=255) ctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa < 2:762722/149‑1 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGtgcg > 2:474522/1‑135 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGtgcg < 1:253507/138‑4 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAAt > 3:1362936/1‑149 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa > 3:280807/1‑147 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa < 4:280807/147‑1 (MQ=255) gATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATcc > 1:1307974/1‑149 (MQ=255) tGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTc > 1:699299/1‑149 (MQ=255) tGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTc > 3:707798/1‑149 (MQ=255) gAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 4:248698/1‑149 (MQ=255) aaGAGGGGGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACCAAATCCTCAcc > 2:253934/1‑149 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag < 4:585483/119‑1 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag > 3:585483/1‑119 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCAcc > 3:942612/1‑149 (MQ=255) agagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCg > 2:1432047/1‑149 (MQ=255) agGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGaa < 3:409371/149‑1 (MQ=255) gCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAagag < 4:1240105/149‑1 (MQ=255) gTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATc < 2:1456536/135‑1 (MQ=255) gTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATc > 1:1456536/1‑135 (MQ=255) tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 2:816105/1‑138 (MQ=255) gATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGt < 3:612560/117‑1 (MQ=255) aTGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc > 4:424477/1‑149 (MQ=255) tGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCt > 4:1077007/1‑149 (MQ=255) tGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGACt < 4:811235/149‑1 (MQ=255) gCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 3:428332/133‑1 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:834420/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc < 4:1362936/149‑1 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 3:497410/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 2:1271709/1‑149 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCga > 3:518601/1‑99 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCga < 4:518601/99‑1 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGc < 3:1001699/149‑1 (MQ=255) aTCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGcc > 2:597651/1‑149 (MQ=255) tCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaag > 2:1034601/1‑149 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag < 2:902228/93‑1 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag > 1:902228/1‑93 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt > 2:1029291/1‑131 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt < 1:1029291/131‑1 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGt < 3:643283/130‑1 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGt > 4:643283/1‑130 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGat > 4:704771/1‑149 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGAGAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg < 2:230591/103‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGt < 4:607750/128‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGatat < 3:710349/149‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGCGGTAATGGCCTTTGACGAACAGGGAGAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg > 1:230591/1‑103 (MQ=255) cgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatc < 3:1420081/149‑1 (MQ=255) cgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatc < 3:248698/149‑1 (MQ=255) aaaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCt < 4:1017300/113‑1 (MQ=255) aTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCt > 2:155469/1‑149 (MQ=255) tgttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTc < 2:949804/149‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa > 1:955274/1‑103 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 4:942612/149‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 1:1432047/149‑1 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 4:739719/105‑1 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 3:739719/1‑105 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCa > 3:1468204/1‑149 (MQ=255) gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc > 2:247563/1‑149 (MQ=255) gTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag < 1:629276/68‑1 (MQ=255) gTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag > 2:629276/1‑68 (MQ=255) cGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 1:1033037/133‑1 (MQ=255) cAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACTTCTTCGCGGTc < 3:839796/149‑1 (MQ=255) cAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTc < 1:155469/149‑1 (MQ=255) tAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 4:473398/1‑149 (MQ=255) aaTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCa > 4:466469/1‑149 (MQ=255) aaTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCa > 1:686145/1‑149 (MQ=255) aTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCAt < 3:1064038/149‑1 (MQ=255) tGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCAtt < 2:1307974/149‑1 (MQ=255) ccTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGaa < 3:473398/149‑1 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt > 4:385806/1‑85 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt < 3:385806/85‑1 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc > 3:671516/1‑121 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc < 4:671516/121‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTg < 3:1009613/145‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 1:597651/149‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 1:1297820/149‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 4:1468204/149‑1 (MQ=255) tGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGc < 2:686145/149‑1 (MQ=255) aCGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAaga < 4:1291956/76‑1 (MQ=255) gAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAagag > 2:956587/1‑144 (MQ=255) gAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGca > 1:1066809/1‑146 (MQ=255) aGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTAcgcg > 4:483136/1‑149 (MQ=255) ccGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAg > 4:364933/1‑149 (MQ=255) cGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAgg > 1:500040/1‑149 (MQ=255) cTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACttt < 2:1234066/149‑1 (MQ=255) cgcgCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTAt < 1:253934/149‑1 (MQ=255) cgcACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCg < 3:990838/149‑1 (MQ=255) ggAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAgg < 3:1239029/135‑1 (MQ=255) | ATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGC > NC_000913/4225169‑4225461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |