| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,225,322 | G→T | 6.0% | E499* (GAG→TAG) ‡ | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,225,322 | 0 | G | T | 6.0% | 535.3 / 18.6 | 149 | E499* (GAG→TAG) ‡ | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (67/73); new base T (4/5); total (71/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
GGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGC > NC_000913/4225174‑4225461 | gggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCg < 4:1303338/149‑1 (MQ=255) ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCga < 4:848180/149‑1 (MQ=255) ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCga < 4:159830/149‑1 (MQ=255) aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGAt > 3:208690/1‑149 (MQ=255) aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGAt > 4:710349/1‑149 (MQ=255) cATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGcc > 3:811235/1‑149 (MQ=255) ttGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt > 2:325770/1‑149 (MQ=255) tGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTc < 1:440970/149‑1 (MQ=255) aaCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:996191/149‑1 (MQ=255) aaCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:692061/149‑1 (MQ=255) tctATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGc < 1:88847/138‑1 (MQ=255) tctATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGc > 2:88847/1‑138 (MQ=255) cttATCTTGATTAAAGGGGGCGGCCATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTa < 2:159875/147‑1 (MQ=255) aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTa < 3:331161/146‑1 (MQ=255) aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTa > 4:331161/1‑146 (MQ=255) tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa > 2:879446/1‑149 (MQ=255) tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGAAAAATCGAGATTTGCCGTCGGGCGTACaaa > 1:949804/1‑149 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGtgcg < 1:253507/138‑4 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGtgcg > 2:253507/1‑135 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGtgcg > 2:474522/1‑135 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAAt > 3:1362936/1‑149 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa < 4:280807/147‑1 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATc < 1:630075/149‑1 (MQ=255) gATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATcc > 1:1307974/1‑149 (MQ=255) gAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 4:248698/1‑149 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag < 4:585483/119‑1 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag > 3:585483/1‑119 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCAcc > 3:942612/1‑149 (MQ=255) gagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGt < 2:1357821/126‑1 (MQ=255) gCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAagag < 4:1240105/149‑1 (MQ=255) gTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATc < 2:1456536/135‑1 (MQ=255) tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 1:816105/138‑1 (MQ=255) tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 2:816105/1‑138 (MQ=255) gATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGt < 3:612560/117‑1 (MQ=255) gATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTCGATTTTCCGt > 4:612560/1‑117 (MQ=255) aTGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc > 4:424477/1‑149 (MQ=255) tGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCt > 4:1077007/1‑149 (MQ=255) gCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGTACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 4:428332/1‑133 (MQ=255) gCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 3:428332/133‑1 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 2:1271709/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 4:839796/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:834420/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc < 4:1362936/149‑1 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 3:497410/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:1434317/1‑149 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCga > 3:518601/1‑99 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGc < 3:1001699/149‑1 (MQ=255) aTCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa < 4:707798/149‑1 (MQ=255) aTCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa > 4:1064038/1‑149 (MQ=255) tCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaag > 2:1034601/1‑149 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt < 1:1029291/131‑1 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt > 2:1029291/1‑131 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGt < 3:643283/130‑1 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGat < 2:1434317/149‑1 (MQ=255) cgcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgaga > 2:153969/1‑92 (MQ=255) cgcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgaga < 1:153969/92‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGatat < 3:710349/149‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGCGGTAATGGCCTTTGACGAACAGGGAGAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg > 1:230591/1‑103 (MQ=255) cgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatc < 3:1420081/149‑1 (MQ=255) gAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatca > 1:1234066/1‑149 (MQ=255) aaaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCt > 3:1017300/1‑113 (MQ=255) aTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCt < 4:497410/149‑1 (MQ=255) tgttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTc < 2:949804/149‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa < 2:955274/103‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaaa > 1:955274/1‑103 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 4:942612/149‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 1:1432047/149‑1 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 3:739719/1‑105 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 4:739719/105‑1 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCa > 3:1468204/1‑149 (MQ=255) gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc > 2:247563/1‑149 (MQ=255) gTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag < 1:629276/68‑1 (MQ=255) gTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCgag > 2:629276/1‑68 (MQ=255) cGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 1:1033037/133‑1 (MQ=255) cAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTc < 1:155469/149‑1 (MQ=255) tAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 4:473398/1‑149 (MQ=255) aTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCAt < 3:1064038/149‑1 (MQ=255) aTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCAt < 3:704771/149‑1 (MQ=255) tGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCAtt < 2:1307974/149‑1 (MQ=255) ggCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGGCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTg > 4:67127/1‑149 (MQ=255) ccTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGaa < 2:699299/149‑1 (MQ=255) ccTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGaa < 3:473398/149‑1 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt > 4:385806/1‑85 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt < 3:385806/85‑1 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc > 3:671516/1‑121 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc < 4:671516/121‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCg > 3:280381/1‑76 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTg > 4:1009613/1‑145 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 4:1468204/149‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 1:597651/149‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 4:554748/149‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCAACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 4:477628/149‑1 (MQ=255) tGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGc < 2:686145/149‑1 (MQ=255) gAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGca > 1:1066809/1‑146 (MQ=255) cGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAgg > 1:500040/1‑149 (MQ=255) cGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAgg > 3:422426/1‑149 (MQ=255) cTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACttt < 2:1234066/149‑1 (MQ=255) cgcgCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTAt < 1:253934/149‑1 (MQ=255) cgcACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCg < 3:990838/149‑1 (MQ=255) ggAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAgg < 3:1239029/135‑1 (MQ=255) | GGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGC > NC_000913/4225174‑4225461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |