| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,225,329 | G→T | 6.1% | C501F (TGC→TTC) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,225,329 | 0 | G | T | 6.1% | 519.0 / 18.4 | 149 | C501F (TGC→TTC) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (65/74); new base T (4/5); total (70/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
CATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAA > NC_000913/4225183‑4225477 | cATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGcc > 3:811235/1‑149 (MQ=255) ttGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGt < 1:611648/149‑1 (MQ=255) ttGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGt > 2:325770/1‑149 (MQ=255) tGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTc < 1:440970/149‑1 (MQ=255) gTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCAATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGT‑ATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCgg < 2:1441270/149‑1 (MQ=255) aaCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGc < 1:996191/149‑1 (MQ=255) aaCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGc < 1:692061/149‑1 (MQ=255) aCTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCg > 2:630075/1‑149 (MQ=255) tctATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGc < 1:88847/138‑1 (MQ=255) tctATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGc > 2:88847/1‑138 (MQ=255) cttATCTTGATTAAAGGGGGCGGCCATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTa < 2:159875/147‑1 (MQ=255) aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTa < 3:331161/146‑1 (MQ=255) aTCTCGATGAAAGAGGGCGTCGATGCCTTAACCCTTCAGCGGAAATTGTGCCGTCCTTCGGGTGCGG‑AATGGTGGTTAAGGCCCTTGACCAACAAGGACAGGCCGATTATCCGCCACCGAAAAACGAGGATTTCCCGCCGGGCggcagg > 1:159875/1‑144 (MQ=255) tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACaaa > 2:879446/1‑149 (MQ=255) tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGAAAAATCGA‑GATTTGCCGTCGGGCGTACaaa > 1:949804/1‑149 (MQ=255) ctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACaaaa < 2:762722/149‑1 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGtgcg > 2:474522/1‑135 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGtgcg < 1:253507/138‑4 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGtgcg < 1:474522/138‑4 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGtgcg > 2:253507/1‑135 (MQ=255) tcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAAt > 3:1362936/1‑149 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACaaaa > 3:280807/1‑147 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACaaaa < 4:280807/147‑1 (MQ=255) gATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATcc > 1:1307974/1‑149 (MQ=255) tGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTc > 3:707798/1‑149 (MQ=255) gAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCa > 4:248698/1‑149 (MQ=255) aaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCAcc > 3:942612/1‑149 (MQ=255) gagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGt < 2:1357821/126‑1 (MQ=255) gagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGt > 1:1357821/1‑126 (MQ=255) agGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGaa < 3:409371/149‑1 (MQ=255) gCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAagag < 4:1240105/149‑1 (MQ=255) gTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATc > 1:1456536/1‑135 (MQ=255) tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCa < 1:816105/138‑1 (MQ=255) tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCa > 2:816105/1‑138 (MQ=255) gATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGt < 3:612560/117‑1 (MQ=255) gATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGCAAATCTC‑GATTTTCCGt > 4:612560/1‑117 (MQ=255) aTGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc > 4:424477/1‑149 (MQ=255) tGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCt > 4:1077007/1‑149 (MQ=255) tGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGACt < 4:811235/149‑1 (MQ=255) gCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCa < 3:428332/133‑1 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 3:497410/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:1434317/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:834420/1‑149 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 2:1271709/1‑149 (MQ=255) aTCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGcc < 3:1077007/149‑1 (MQ=255) aTCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa < 4:1468128/149‑1 (MQ=255) aTCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa < 4:707798/149‑1 (MQ=255) aTCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa < 4:8737/149‑1 (MQ=255) aTCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa > 4:1064038/1‑149 (MQ=255) tCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaag > 2:1034601/1‑149 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt < 1:1029291/131‑1 (MQ=255) aCGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGat < 2:1434317/149‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGt < 4:607750/128‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGt > 3:607750/1‑128 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGatat < 3:710349/149‑1 (MQ=255) gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGCGGTAATGGCCTTTGACGAACAGGGAGAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCggg > 1:230591/1‑103 (MQ=255) cgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatc < 3:1420081/149‑1 (MQ=255) cgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatc < 3:248698/149‑1 (MQ=255) cgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatc > 3:477628/1‑149 (MQ=255) gAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatca > 1:1234066/1‑149 (MQ=255) aaaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCt > 3:1017300/1‑113 (MQ=255) aTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCt > 2:155469/1‑149 (MQ=255) aTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCt < 4:497410/149‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACaaaa < 2:955274/103‑1 (MQ=255) ttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 4:942612/149‑1 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCa > 3:739719/1‑105 (MQ=255) gTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCa > 3:1468204/1‑149 (MQ=255) gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc > 2:247563/1‑149 (MQ=255) aCGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATc < 4:738581/149‑1 (MQ=255) cGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa < 1:1033037/133‑1 (MQ=255) cGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGa > 2:1033037/1‑133 (MQ=255) cAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACTTCTTCGCGGTc < 3:839796/149‑1 (MQ=255) tAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 4:473398/1‑149 (MQ=255) aaTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCa < 3:466469/149‑1 (MQ=255) aaTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCa > 1:686145/1‑149 (MQ=255) aaTGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCa > 4:466469/1‑149 (MQ=255) tGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCAtt < 2:1307974/149‑1 (MQ=255) ggCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGGCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTg > 4:67127/1‑149 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt < 3:385806/85‑1 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc > 3:671516/1‑121 (MQ=255) cTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAAc < 4:671516/121‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCg < 4:280381/76‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTg < 3:1009613/145‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTg > 4:1009613/1‑145 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 4:1468204/149‑1 (MQ=255) tttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAaga < 1:597651/149‑1 (MQ=255) ttGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGGCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAagag < 3:67127/149‑1 (MQ=255) tGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGc < 2:686145/149‑1 (MQ=255) aCGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAaga < 4:1291956/76‑1 (MQ=255) gAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAagag < 1:956587/144‑1 (MQ=255) gAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAagag > 2:956587/1‑144 (MQ=255) gAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGca > 1:1066809/1‑146 (MQ=255) aGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTAcgcg > 4:483136/1‑149 (MQ=255) ccGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAg > 4:364933/1‑149 (MQ=255) cTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACttt < 2:1234066/149‑1 (MQ=255) cgcgCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTAt < 1:253934/149‑1 (MQ=255) cgcACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCg < 3:990838/149‑1 (MQ=255) cGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGgcgc < 4:422426/149‑1 (MQ=255) ggAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAgg < 3:1239029/135‑1 (MQ=255) aTTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAagag > 4:829721/1‑105 (MQ=255) | CATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGA‑GATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAA > NC_000913/4225183‑4225477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |