Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,241,716 C→G 6.9% C180S (TGC→TCC) ‡ xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,241,7160CG 6.9% 251.6 / 8.5 72C180S (TGC→TCC) ‡xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base C (33/34);  new base G (2/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

CGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAAT  >  NC_000913/4241568‑4241864
                                                                                                                                                    |                                                                                                                                                    
cgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGc                                                                                                                                                      >  4:892004/1‑149 (MQ=255)
cgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGc                                                                                                                                                      <  4:569220/149‑1 (MQ=255)
         ccagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTa                                                                                                                                             <  1:879541/149‑1 (MQ=255)
         ccagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTa                                                                                                                                             >  2:387742/1‑149 (MQ=255)
           agcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaaa                                                                                                                                           <  3:237597/149‑1 (MQ=255)
                  aCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGtt                                                                                                                                       >  2:663482/1‑146 (MQ=255)
                  aCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGtt                                                                                                                                       <  1:663482/146‑1 (MQ=255)
                                     agcagcATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTg                                                                                                                 <  3:892004/149‑1 (MQ=255)
                                        agcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGtt                                                                                                              <  3:886090/149‑1 (MQ=255)
                                           aTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaa                                                                                                           >  2:1127482/1‑149 (MQ=255)
                                             tAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAag                                                                                                         >  2:908341/1‑149 (MQ=255)
                                                gAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAaataat                                                                                                                          <  1:216400/129‑1 (MQ=255)
                                                gAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAaataat                                                                                                                          >  2:216400/1‑129 (MQ=255)
                                                gAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAgaga                                                                                                      <  1:1127482/149‑1 (MQ=255)
                                                gAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAgaga                                                                                                      <  1:908341/149‑1 (MQ=255)
                                                 aaCAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGt                                                                                                               <  1:452107/139‑1 (MQ=255)
                                                 aaCAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGt                                                                                                               >  2:452107/1‑139 (MQ=255)
                                                          gCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAAc                                                                                                                                               >  3:371607/1‑98 (MQ=255)
                                                          gCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAAc                                                                                                                                               <  4:371607/98‑1 (MQ=255)
                                                              ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTattt                                                                                                                                          >  1:654059/1‑96 (MQ=255)
                                                              ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTattt                                                                                                                                          <  2:654059/99‑4 (MQ=255)
                                                                     ttCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAg                                                                                 >  2:15496/1‑149 (MQ=255)
                                                                          aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGa                                                                                  >  2:610808/1‑143 (MQ=255)
                                                                          aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGa                                                                                  <  1:610808/143‑1 (MQ=255)
                                                                          aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGa                                                                            <  1:15496/149‑1 (MQ=255)
                                                                              aaaCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaaaa                                                                                                                                          <  2:888759/83‑1 (MQ=255)
                                                                              aaaCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaaaa                                                                                                                                          >  1:888759/1‑83 (MQ=255)
                                                                                   tataACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                   <  1:387742/149‑1 (MQ=255)
                                                                                   tataACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                   >  4:741791/1‑149 (MQ=255)
                                                                                     taACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATc                                                                                                                         <  4:739037/93‑1 (MQ=255)
                                                                                     taACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATc                                                                                                                         >  3:739037/1‑93 (MQ=255)
                                                                                      aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCa                                                                >  1:176467/1‑149 (MQ=255)
                                                                                          ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                        >  3:1332919/1‑105 (MQ=255)
                                                                                          ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                        <  4:1332919/105‑1 (MQ=255)
                                                                                             gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACa                                                         <  2:176467/149‑1 (MQ=255)
                                                                                               cGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                          >  1:270429/1‑98 (MQ=255)
                                                                                               cGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                          <  2:270429/98‑1 (MQ=255)
                                                                                                 tCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAATAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTgg                                                     >  1:1239027/1‑149 (MQ=255)
                                                                                                     tATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                          <  4:760258/92‑1 (MQ=255)
                                                                                                     tATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                          >  3:760258/1‑92 (MQ=255)
                                                                                                       ttCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGa                                                                                  >  3:531383/1‑114 (MQ=255)
                                                                                                       ttCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGa                                                                                  <  4:531383/114‑1 (MQ=255)
                                                                                                       ttCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGagag                                               >  1:1110525/1‑149 (MQ=255)
                                                                                                       ttCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGagag                                               >  3:503517/1‑149 (MQ=255)
                                                                                                           gccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCt                                                                               >  4:418128/1‑113 (MQ=255)
                                                                                                           gccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCt                                                                               <  3:418128/113‑1 (MQ=255)
                                                                                                           gccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                           >  1:169601/1‑149 (MQ=255)
                                                                                                            ccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCgag                                                 >  4:1149186/1‑142 (MQ=255)
                                                                                                            ccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCgag                                                 <  3:1149186/142‑1 (MQ=255)
                                                                                                              agcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAg                                        <  3:741791/149‑1 (MQ=255)
                                                                                                               gcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGaa                                                                                       >  3:152067/1‑101 (MQ=255)
                                                                                                               gcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGaa                                                                                       <  4:152067/101‑1 (MQ=255)
                                                                                                                        aCCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCaa                              <  2:1110525/149‑1 (MQ=255)
                                                                                                                         ccGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGa                                         >  4:1295253/1‑137 (MQ=255)
                                                                                                                         ccGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTACGCAATATACATTGGCGAGAGCATTGa                                         <  3:1295253/137‑1 (MQ=255)
                                                                                                                           ggAAAGGGCAATAAAATAGTTTACGCAATAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGc                           <  2:1239027/149‑1 (MQ=255)
                                                                                                                              aCGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCaa                        <  4:503517/149‑1 (MQ=255)
                                                                                                                                     aTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGcc                 >  4:1381044/1‑149 (MQ=255)
                                                                                                                                        aaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                   <  4:248639/96‑1 (MQ=255)
                                                                                                                                        aaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                   >  1:661405/1‑96 (MQ=255)
                                                                                                                                        aaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                   >  3:248639/1‑96 (MQ=255)
                                                                                                                                        aaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                   <  2:661405/96‑1 (MQ=255)
                                                                                                                                        aaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGa                                         >  2:943314/1‑122 (MQ=255)
                                                                                                                                        aaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTCCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGa                                         <  1:943314/122‑1 (MQ=255)
                                                                                                                                         aaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGc                                                    <  2:951973/110‑1 (MQ=255)
                                                                                                                                         aaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGc                                                    >  1:951973/1‑110 (MQ=255)
                                                                                                                                              tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                        <  1:144153/53‑1 (MQ=255)
                                                                                                                                              tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                        >  2:313422/1‑53 (MQ=255)
                                                                                                                                              tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                        <  1:313422/53‑1 (MQ=255)
                                                                                                                                              tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                        >  2:144153/1‑53 (MQ=255)
                                                                                                                                              tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCt                                     >  1:1305448/1‑120 (MQ=255)
                                                                                                                                              tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCt                                     <  2:1305448/120‑1 (MQ=255)
                                                                                                                                                tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                          >  1:528574/1‑49 (MQ=255)
                                                                                                                                                tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                          <  2:528574/49‑1 (MQ=255)
                                                                                                                                                    cAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAg                                        >  3:1276795/1‑111 (MQ=255)
                                                                                                                                                    cAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAg                                        <  4:1276795/111‑1 (MQ=255)
                                                                                                                                                    cAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaat  >  2:1454628/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAAT  >  NC_000913/4241568‑4241864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: