Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,317,509 G→C 6.1% A129A (GCC→GCG phnL ← ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,317,5090GC 6.1% 231.0 / 4.9 66A129A (GCC→GCGphnLribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base C (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGATTTCCACCACTTTGCGCGCT  >  NC_000913/4317367‑4317656
                                                                                                                                              |                                                                                                                                                   
tAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTa                                                                                                                                               <  1:450228/149‑1 (MQ=255)
 aGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAg                                                                                                                                              <  2:450715/149‑1 (MQ=255)
  ggTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgc                                                                                                                                             <  4:375461/149‑1 (MQ=255)
        tCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCCGCTAAAGCCgcgcg                                                                                                                                       >  2:289573/1‑148 (MQ=255)
          aaGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCCGCTAAAGCCgcgcgtc                                                                                                                                     <  1:289573/149‑4 (MQ=255)
                  gAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAg                                                                                                                                              <  2:477890/132‑1 (MQ=255)
                  gAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAg                                                                                                                                              >  1:477890/1‑132 (MQ=255)
                  gAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCCGCTAAAGCCgcgcgt                                                                                                                                      <  2:671823/140‑3 (MQ=255)
                  gAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCCGCTAAAGCCgcgcgt                                                                                                                                      >  1:671823/1‑138 (MQ=255)
                          tAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGcc                                                                                                                     <  1:160698/149‑1 (MQ=255)
                                  gaTAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGa                                                                                                             >  1:207536/1‑149 (MQ=255)
                                    tAAAGCCGCGGGCGATGTTGACGCGCTGCTGTGCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgc                                                                                                                                             >  4:1252402/1‑115 (MQ=255)
                                    tAAAGCCGCGAGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgc                                                                                                                                             <  3:1252402/115‑1 (MQ=255)
                                     aaaGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGagca                                                                                                          <  2:207536/149‑1 (MQ=255)
                                     aaaGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGagca                                                                                                          >  2:982118/1‑149 (MQ=255)
                                       aGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGc                                                                                                                                    >  2:195031/1‑121 (MQ=255)
                                       aGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGc                                                                                                                                    <  1:195031/121‑1 (MQ=255)
                                        gCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAACGTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCg                                                                                                       >  1:225863/1‑149 (MQ=255)
                                        gCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCg                                                                                                       >  1:419659/1‑149 (MQ=255)
                                              ggCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgcggc                                                                                                                                          >  2:1009423/1‑108 (MQ=255)
                                              ggCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgcggc                                                                                                                                          <  1:1009423/108‑1 (MQ=255)
                                                       gACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGc                                                                                                     <  2:122909/136‑1 (MQ=255)
                                                       gACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGc                                                                                                     >  1:122909/1‑136 (MQ=255)
                                                          gcgcTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCa                                                                                     <  1:982118/149‑1 (MQ=255)
                                                                  gTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGagcagc                                                                                                        >  2:1319337/1‑122 (MQ=255)
                                                                  gTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGagcagc                                                                                                        <  1:1319337/122‑1 (MQ=255)
                                                                  gTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTga                                                                              >  2:1215868/1‑148 (MQ=255)
                                                                  gTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTga                                                                              <  1:1215868/148‑1 (MQ=255)
                                                                      gCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGcc                                                                                                                     <  4:681361/105‑1 (MQ=255)
                                                                      gCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGcc                                                                                                                     >  3:681361/1‑105 (MQ=255)
                                                                                aaTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAc                                                               >  3:814667/1‑149 (MQ=255)
                                                                                  tGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc                                                             <  2:225863/149‑1 (MQ=255)
                                                                                  tGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc                                                             <  1:1005997/149‑1 (MQ=255)
                                                                                  tGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc                                                             <  2:419659/149‑1 (MQ=255)
                                                                                  tGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc                                                             >  2:551419/1‑149 (MQ=255)
                                                                                    tCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGcc                                                                                                                     <  3:29359/91‑1 (MQ=255)
                                                                                    tCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGcc                                                                                                                     >  4:29359/1‑91 (MQ=255)
                                                                                            gccaggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTg                                                   >  2:935096/1‑149 (MQ=255)
                                                                                               aggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCt                                                >  3:195469/1‑149 (MQ=255)
                                                                                                     cacaGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCa                                          >  4:275905/1‑149 (MQ=255)
                                                                                                      acaGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAg                                         >  1:408176/1‑149 (MQ=255)
                                                                                                      acaGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAg                                         <  4:814667/149‑1 (MQ=255)
                                                                                                          gCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGa                                     >  2:233852/1‑149 (MQ=255)
                                                                                                             ttCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACgg                                  >  2:1217117/1‑149 (MQ=255)
                                                                                                             ttCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACgg                                  >  1:923013/1‑149 (MQ=255)
                                                                                                               ccGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACggtg                                <  1:935096/149‑1 (MQ=255)
                                                                                                                cGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTgg                                                  <  3:768963/130‑1 (MQ=255)
                                                                                                                cGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTgg                                                  >  4:768963/1‑130 (MQ=255)
                                                                                                                  gCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGtt                             >  4:431475/1‑149 (MQ=255)
                                                                                                                  gCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGtt                             >  4:844764/1‑149 (MQ=255)
                                                                                                                   cACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGttt                            <  1:551419/149‑1 (MQ=255)
                                                                                                                      gTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGc                         >  2:543627/1‑149 (MQ=255)
                                                                                                                          aGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGAt                     <  2:408176/149‑1 (MQ=255)
                                                                                                                            gCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGc                                                                                                     >  3:215804/1‑67 (MQ=255)
                                                                                                                            gCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGc                                                                                                     <  4:215804/67‑1 (MQ=255)
                                                                                                                            gCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAAc                                                     >  2:943210/1‑115 (MQ=255)
                                                                                                                            gCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAAc                                                     <  1:943210/115‑1 (MQ=255)
                                                                                                                            gCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGAttt                   <  2:923013/149‑1 (MQ=255)
                                                                                                                                gTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCgg                                                                     <  2:1464943/95‑1 (MQ=255)
                                                                                                                                gTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCgg                                                                     >  1:1464943/1‑95 (MQ=255)
                                                                                                                                 tGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGATTTccacc              >  3:1054296/1‑149 (MQ=255)
                                                                                                                                    gaagaCGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATc                                                                                               >  3:532138/1‑65 (MQ=255)
                                                                                                                                    gaagaCGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATc                                                                                               <  4:532138/65‑1 (MQ=255)
                                                                                                                                       gaCGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc                                                             <  3:85850/96‑1 (MQ=255)
                                                                                                                                       gaCGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc                                                             >  4:85850/1‑96 (MQ=255)
                                                                                                                                       gaCGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGATTTCCACCACTTTg        >  3:1132171/1‑149 (MQ=255)
                                                                                                                                          gcgcgGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGATTTCCACCACTTTgcgc     >  1:1120593/1‑149 (MQ=255)
                                                                                                                                             cgGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGATTTCCACCACTTTGCGCGCt  >  1:755610/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACTGGCTCACCCAGCCGACGGTGGTTTTGCGGATTTCCACCACTTTGCGCGCT  >  NC_000913/4317367‑4317656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: