Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 378,405 C→T 5.3% intergenic (‑37/+57) frmB ← / ← frmA S‑formylglutathione hydrolase/alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913378,4050CT 5.3% 518.6 / 10.4 150intergenic (‑37/+57)frmB/frmAS‑formylglutathione hydrolase/alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (73/69);  new base T (2/6);  total (75/75)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 152 total aligned reads displayed.

GTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGT  >  NC_000913/378263‑378548
                                                                                                                                              |                                                                                                                                               
  gggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCGGTCCggggga                                                                                                                                         <  3:625548/149‑3 (MQ=255)
  gggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggggga                                                                                                                                         <  1:674559/149‑3 (MQ=255)
   ggAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgaga                                                                                                                                        >  3:226344/1‑149 (MQ=255)
         aGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTa                                                                                                                                  >  3:801811/1‑149 (MQ=255)
          gACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGCGAGAGTATTaa                                                                                                                                 <  4:130177/149‑1 (MQ=255)
                gACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                            >  2:1062606/1‑132 (MQ=255)
                gACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                            <  1:1062606/132‑1 (MQ=255)
                   attcattTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGTGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTa                                                                                                                                     <  2:1071344/136‑1 (MQ=255)
                   attcattTCACAATTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTa                                                                                                                                     >  1:1071344/1‑136 (MQ=255)
                        tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCt                                                                                                                     <  2:155065/147‑1 (MQ=255)
                        tttcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCt                                                                                                                     >  1:155065/1‑147 (MQ=255)
                          tcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTa                                                                                                                                  <  1:471673/132‑1 (MQ=255)
                          tcacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAg                                                                                                                 <  4:226344/149‑1 (MQ=255)
                           cacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGt                                                                                                                >  2:16397/1‑149 (MQ=255)
                           cacatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGt                                                                                                                <  1:1386429/149‑1 (MQ=255)
                            acatttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGt                                                                                                                >  4:148729/1‑148 (MQ=255)
                               tttcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGAc                                                                                                            >  2:1227917/1‑149 (MQ=255)
                                 tcaGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgg                                                                                                          <  1:1227917/149‑1 (MQ=255)
                                    gTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGt                                                                                                       >  1:1314452/1‑149 (MQ=255)
                                           ggAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgaga                                                                                                                                        >  3:1207623/1‑109 (MQ=255)
                                           ggAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTg                                                                                                >  3:1411964/1‑149 (MQ=255)
                                           ggAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTg                                                                                                >  1:1036184/1‑149 (MQ=255)
                                                     cGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                      <  2:1036184/149‑1 (MQ=255)
                                                        tACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGaa                                                                                                                  >  1:612631/1‑118 (MQ=255)
                                                           atatTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAAc                                                                                                                             >  3:899498/1‑104 (MQ=255)
                                                            tatTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCgg                                                                                             >  3:1358020/1‑135 (MQ=255)
                                                            tatTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCgg                                                                                             <  4:1358020/135‑1 (MQ=255)
                                                              ttttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                      >  3:1170345/1‑140 (MQ=255)
                                                              ttttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCa                                                                                      <  4:1170345/140‑1 (MQ=255)
                                                              ttttGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAAt                                                                             >  3:1326152/1‑149 (MQ=255)
                                                                    cAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggg                                                                                                                                            <  2:1304649/80‑1 (MQ=255)
                                                                    cAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGGCAGCCGTCCggg                                                                                                                                            >  1:1304649/1‑80 (MQ=255)
                                                                    cAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGt                                                                       >  1:721278/1‑149 (MQ=255)
                                                                     aGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGtt                                                                      >  3:361552/1‑149 (MQ=255)
                                                                      gccgccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTc                                                                     <  4:361552/149‑1 (MQ=255)
                                                                          ccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGgaga                                                                                                                                        >  1:509914/1‑78 (MQ=255)
                                                                          ccAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAAt                                                                 <  1:16397/149‑1 (MQ=255)
                                                                           cAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACggg                                                                                                         >  4:1249466/1‑108 (MQ=255)
                                                                                 tGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTg                                                          >  2:561569/1‑149 (MQ=255)
                                                                                   aCATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGcc                                                        >  3:198377/1‑149 (MQ=255)
                                                                                    cATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                            >  2:279215/1‑64 (MQ=255)
                                                                                    cATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                            <  1:279215/64‑1 (MQ=255)
                                                                                      tGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGAGAGAGTATTAAGAACCCCCGCCTGaa                                                                                                                  <  4:483760/88‑1 (MQ=255)
                                                                                      tGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGAGAGAGTATTAAGAACCCCCGCCTGaa                                                                                                                  >  3:483760/1‑88 (MQ=255)
                                                                                       gTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGa                                                                                                             <  2:1458001/92‑1 (MQ=255)
                                                                                       gTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGcc                                                        <  2:873797/145‑1 (MQ=255)
                                                                                       gTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGcc                                                        >  1:873797/1‑145 (MQ=255)
                                                                                        tttttCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTaa                                                                                  <  3:312132/118‑1 (MQ=255)
                                                                                        tttttCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTaa                                                                                  >  4:312132/1‑118 (MQ=255)
                                                                                        tttttCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCAt                                                   >  1:274959/1‑149 (MQ=255)
                                                                                              aaTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                            <  4:564550/54‑1 (MQ=255)
                                                                                              aaTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCggg                                                                                                                                            >  3:564550/1‑54 (MQ=255)
                                                                                              aaTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCa                                                >  3:1242203/1‑146 (MQ=255)
                                                                                              aaTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCa                                             >  3:1116087/1‑149 (MQ=255)
                                                                                              aaTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCa                                             >  1:906710/1‑149 (MQ=255)
                                                                                               aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTAc                                                                          >  4:1391189/1‑119 (MQ=255)
                                                                                               aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCAt                                                   >  3:149627/1‑142 (MQ=255)
                                                                                               aTGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCAt                                                   <  4:149627/142‑1 (MQ=255)
                                                                                                tGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                        <  4:1217879/88‑1 (MQ=255)
                                                                                                tGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACgggg                                                                                                        >  3:1217879/1‑88 (MQ=255)
                                                                                                tGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAttt                                                           <  2:478532/133‑1 (MQ=255)
                                                                                                tGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAgg                                           <  2:906710/149‑1 (MQ=255)
                                                                                                    ttCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATgg                                                               <  4:772819/125‑1 (MQ=255)
                                                                                                     tCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGaa                                      <  2:721278/149‑1 (MQ=255)
                                                                                                       cATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAgg                                    <  4:1116087/149‑1 (MQ=255)
                                                                                                        aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTaa                                                                                  >  2:408058/1‑102 (MQ=255)
                                                                                                        aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTaa                                                                                  <  1:408058/102‑1 (MQ=255)
                                                                                                        aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATc                                              >  2:1142124/1‑138 (MQ=255)
                                                                                                        aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATc                                              <  1:1142124/138‑1 (MQ=255)
                                                                                                        aTCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGc                                   >  4:1205041/1‑149 (MQ=255)
                                                                                                         tCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCt                                                       >  2:875330/1‑128 (MQ=255)
                                                                                                         tCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCt                                                       <  1:875330/128‑1 (MQ=255)
                                                                                                           aGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAAt                                                                 >  1:1417641/1‑116 (MQ=255)
                                                                                                           aGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAAt                                                                 <  2:1417641/116‑1 (MQ=255)
                                                                                                            gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAttt                                                           <  4:1215798/121‑1 (MQ=255)
                                                                                                            gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTc                                                     <  4:1030200/127‑1 (MQ=255)
                                                                                                            gTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTc                                                     >  3:1030200/1‑127 (MQ=255)
                                                                                                               aTTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGAt                                                             <  2:67028/116‑1 (MQ=255)
                                                                                                                   tctcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAttt                        <  2:1314452/149‑1 (MQ=255)
                                                                                                                     tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGa                                                              >  3:677740/1‑109 (MQ=255)
                                                                                                                     tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                      >  1:544378/1‑149 (MQ=255)
                                                                                                                     tcAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                      >  1:1018844/1‑149 (MQ=255)
                                                                                                                      cAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCAt                     >  2:396168/1‑149 (MQ=255)
                                                                                                                        gggTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATc                    >  4:837379/1‑148 (MQ=255)
                                                                                                                        gggTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATc                    >  4:1140879/1‑148 (MQ=255)
                                                                                                                        gggTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATc                    <  3:1140879/148‑1 (MQ=255)
                                                                                                                          gTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAg                 <  2:544378/149‑1 (MQ=255)
                                                                                                                           ttAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAgg                >  1:603724/1‑149 (MQ=255)
                                                                                                                               tCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                      >  3:700878/1‑139 (MQ=255)
                                                                                                                               tCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCa                      <  4:700878/139‑1 (MQ=255)
                                                                                                                               ctccccGGGCAGCCGTCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCg                                                                                              <  3:234838/65‑1 (MQ=37)
                                                                                                                               ctccccGGGCAGCCGTCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCg                                                                                              >  4:234838/3‑67 (MQ=37)
                                                                                                                                tccccGGGCAGCCGTCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATc                    <  3:1410755/139‑1 (MQ=255)
                                                                                                                                 ccccGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATg          >  2:495256/1‑149 (MQ=255)
                                                                                                                                         ggCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAAc                                                                                                   >  3:423985/1‑52 (MQ=255)
                                                                                                                                         ggCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAAc                                                                                                   <  4:423985/52‑1 (MQ=255)
                                                                                                                                         ggCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAgg                                           <  2:98846/108‑1 (MQ=255)
                                                                                                                                         ggCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAgg                                           >  1:98846/1‑108 (MQ=255)
                                                                                                                                         ggCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGt  <  2:274959/149‑1 (MQ=255)
                                                                                                                                              cccGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAAtt                         <  4:667544/121‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GTGGGAGATAGACGCCGACATTCATTTCACATTTCAGTGATTGGGAATAATGCCGATACATATTTTGCCAGCCGCCAAAGCTGACATGTTTTTCAATGAGTTCCATCAGTCATTATCTCAGGGTTAATCCCCCGGACGGCTGCCCGGGGAGAGTATTAAGAACCCCCGCCTGAAGTGGACGGGGTAAACCTGCGGGAAATCAGTAACGAATTACGGTTCGAATGGATTTGCCTTCATGCATCAGGTCGAAGGCGTCATTAATTTCATCCAGGCTCATGGTATGCGT  >  NC_000913/378263‑378548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: