Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,633 C→T 5.7% G27G (GGC→GGT ecnB → entericidin B membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,6330CT 5.7% 522.0 / 13.4 156G27G (GGC→GGTecnBentericidin B membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (75/72);  new base T (5/4);  total (80/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

Only 100 of 156 total aligned reads displayed.

CGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCC  >  NC_000913/4376488‑4376775
                                                                                                                                                 |                                                                                                                                              
cGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGtt                                                                                                                                             <  3:557427/149‑1 (MQ=255)
     ttgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGa                                                                                                                                        >  2:776575/1‑149 (MQ=255)
        tCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGa                                                                                                                                     <  1:1234391/149‑1 (MQ=255)
              attaATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTc                                                                                                                               >  1:245823/1‑149 (MQ=255)
                 aaTTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                            <  1:776575/149‑1 (MQ=255)
                   tttGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACGCCACGCGTGGTGTTgc                                                                                                                                           >  4:1099542/1‑131 (MQ=255)
                   tttGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg                                                                                                                                            <  3:1337247/131‑1 (MQ=255)
                           aGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTc                                                                                                                               <  1:1140819/136‑1 (MQ=255)
                           aGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTc                                                                                                                               >  2:1140819/1‑136 (MQ=255)
                             gCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTc                                                                                                                               >  1:342292/1‑134 (MQ=255)
                             gCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTc                                                                                                                               <  2:342292/134‑1 (MQ=255)
                             gCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGAt                                                                                                                           >  4:397096/1‑138 (MQ=255)
                               aaaCATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                            <  3:582155/135‑1 (MQ=255)
                                 aCATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTaa                                                                                                                   >  3:1020078/1‑142 (MQ=255)
                                 aCATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTaa                                                                                                                   <  4:1020078/142‑1 (MQ=255)
                                 aCATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAtt                                                                                                            >  4:1156359/1‑149 (MQ=255)
                                     aaataaCATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg                                                                                                                                            >  2:949649/1‑113 (MQ=255)
                                            aTTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg                                                                                                                                           >  2:1266880/1‑107 (MQ=255)
                                             ttACCTAAAAGGAAGACGTTATGGTGCAGAAGACAATGGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAATGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgcagca                                                                                                >  2:1056996/1‑149 (MQ=255)
                                             ttACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGt                                                                                                                                              >  2:589465/1‑103 (MQ=255)
                                             ttACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGt                                                                                                                                              <  1:589465/103‑1 (MQ=255)
                                              tACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCaa                                                                                               <  3:1156359/149‑1 (MQ=255)
                                                 cTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGa                                                                                                                                        >  4:482711/1‑105 (MQ=255)
                                                 cTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGa                                                                                                                                        <  3:482711/105‑1 (MQ=255)
                                                 cTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAtt                                                                                                            >  1:542832/1‑133 (MQ=255)
                                                 cTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGa                                                                                            >  1:1438190/1‑149 (MQ=255)
                                                  tAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAc                                                                                                                  >  3:433439/1‑126 (MQ=255)
                                                   aaaaGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg                                                                                                                                           <  3:372703/100‑1 (MQ=255)
                                                   aaaaGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg                                                                                                                                           <  1:48083/100‑1 (MQ=255)
                                                      aGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAc                                                                                                                  <  1:154183/122‑1 (MQ=255)
                                                      aGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAc                                                                                                                  >  2:154183/1‑122 (MQ=255)
                                                      aGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAgcg                                                                                       >  4:272255/1‑149 (MQ=255)
                                                             cGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaat                                                                                <  4:796546/149‑1 (MQ=255)
                                                                  tGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt                                                                                                                                <  1:674158/96‑1 (MQ=255)
                                                                   ggTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaataagcaa                                                                          >  2:515952/1‑149 (MQ=255)
                                                                    gTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                            >  1:153334/1‑98 (MQ=255)
                                                                      gaagaaGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAgcaataagcaataa                                                                       <  3:1119808/149‑1 (MQ=255)
                                                                        agaagtCAATTGCGGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACg                                                                     <  4:1023570/149‑1 (MQ=255)
                                                                         gaagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAAc                                                                      <  4:1262779/147‑1 (MQ=255)
                                                                         gaagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAAc                                                                      >  3:1262779/1‑147 (MQ=255)
                                                                         gaagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACgg                                                                    >  2:201855/1‑149 (MQ=255)
                                                                          aagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgca                                                                                                   >  4:572915/1‑117 (MQ=255)
                                                                          aagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAAc                                                                      <  1:926646/146‑1 (MQ=255)
                                                                            gaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa                                                                                                                                       <  2:821925/79‑1 (MQ=255)
                                                                            gaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa                                                                                                                                       >  1:821925/1‑79 (MQ=255)
                                                                             aCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTc                                                                                                          <  4:72664/107‑1 (MQ=255)
                                                                               aaTTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGAc                                                              >  2:6175/1‑149 (MQ=255)
                                                                                aTTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACa                                                             <  3:272255/149‑1 (MQ=255)
                                                                                   gCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTa                                                                  >  1:1136430/1‑141 (MQ=255)
                                                                                   gCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTa                                                                  <  2:1136430/141‑1 (MQ=255)
                                                                                     aGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgt                                                        <  1:1056996/149‑1 (MQ=255)
                                                                                      gCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgtg                                                       <  3:1051641/149‑1 (MQ=255)
                                                                                         aTCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAcgc                                                                                                                >  4:1358265/1‑89 (MQ=255)
                                                                                         aTCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCg                                                    >  3:401962/1‑149 (MQ=255)
                                                                                            ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAcgc                                                                                                                >  4:313027/1‑86 (MQ=255)
                                                                                            ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgca                                                                                                   >  2:229316/1‑99 (MQ=255)
                                                                                            ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTc                                                     <  3:1443961/145‑1 (MQ=255)
                                                                                            ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGc                                                 >  2:1459854/1‑149 (MQ=255)
                                                                                                ctgttctgGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCgtt                                             >  3:335889/1‑149 (MQ=255)
                                                                                                 tgttctgGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                            <  2:1365306/69‑1 (MQ=255)
                                                                                                 tgttctgGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                            <  4:1130490/69‑1 (MQ=255)
                                                                                                 tgttctgGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                            >  1:1365306/1‑69 (MQ=255)
                                                                                                    tctgGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCgtttgtt                                         <  3:872511/149‑1 (MQ=255)
                                                                                                     ctgGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCgtttgttt                                        <  1:201855/149‑1 (MQ=255)
                                                                                                        gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCt                                                                                                                              <  3:33806/60‑1 (MQ=255)
                                                                                                        gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCt                                                                                                                              >  4:33806/1‑60 (MQ=255)
                                                                                                        gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgt                                                        >  3:748553/1‑130 (MQ=255)
                                                                                                        gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgt                                                        <  2:101040/130‑1 (MQ=255)
                                                                                                        gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgt                                                        >  1:101040/1‑130 (MQ=255)
                                                                                                            tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCgtttgtt                                         >  2:365662/1‑141 (MQ=255)
                                                                                                            tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCgtttgtt                                         >  1:697031/1‑141 (MQ=255)
                                                                                                            tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGtttt                                       >  1:495867/1‑143 (MQ=255)
                                                                                                            tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGtttt                                       <  2:495867/143‑1 (MQ=255)
                                                                                                            tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCt                                   <  4:364849/147‑1 (MQ=255)
                                                                                                                 aCAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAg                            >  3:1144915/1‑149 (MQ=255)
                                                                                                                  cAGGATTAACTGCCTGCAACACCTCGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATCAGCAATAACGGt                                                                   <  2:1464948/109‑1 (MQ=255)
                                                                                                                          aCTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGaaa                                                                                          <  2:1259527/78‑1 (MQ=255)
                                                                                                                          aCTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaataa                                                                              >  1:1010837/1‑90 (MQ=255)
                                                                                                                          aCTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaataa                                                                              <  2:1010837/90‑1 (MQ=255)
                                                                                                                           ctgcctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaataag                                                                             >  2:1062905/1‑90 (MQ=255)
                                                                                                                           ctgcctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaataag                                                                             <  1:1062905/90‑1 (MQ=255)
                                                                                                                            tgcctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTa                 >  4:1369848/1‑149 (MQ=255)
                                                                                                                              tcaccAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAg                    >  3:678724/5‑144 (MQ=255)
                                                                                                                              tcaccAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAg                    <  4:678724/140‑1 (MQ=255)
                                                                                                                                tgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAg             >  2:68834/1‑149 (MQ=255)
                                                                                                                                tgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAg             >  4:748916/1‑149 (MQ=255)
                                                                                                                                 gcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACgg                                                                    >  2:171267/1‑93 (MQ=255)
                                                                                                                                 gcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGt            >  1:124508/1‑149 (MQ=255)
                                                                                                                                  cAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTa                                                                  <  4:881680/94‑1 (MQ=255)
                                                                                                                                  cAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTa                                                                  >  3:881680/1‑94 (MQ=255)
                                                                                                                                  cAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGtt           >  2:894714/1‑149 (MQ=255)
                                                                                                                                  cAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAg                                                            >  4:436087/1‑100 (MQ=255)
                                                                                                                                   aaCACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGt                                                   <  2:287590/108‑1 (MQ=255)
                                                                                                                                   aaCACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGt                                                   >  1:287590/1‑108 (MQ=255)
                                                                                                                                      accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCgtttgtt                                         >  1:178539/1‑115 (MQ=255)
                                                                                                                                      accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGaaa       <  4:401962/149‑1 (MQ=255)
                                                                                                                                      accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGaaa       <  2:739279/149‑1 (MQ=255)
                                                                                                                                         acGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTgg                                                                                                       >  4:1090359/1‑50 (MQ=255)
                                                                                                                                         acGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTgg                                                                                                       <  3:1090359/50‑1 (MQ=255)
                                                                                                                                          cgcgTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTcc   <  1:1265479/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCC  >  NC_000913/4376488‑4376775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: