Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,381,396 G→C 6.8% P308R (CCG→CGG)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,381,3960GC 6.8% 574.3 / 26.4 162P308R (CCG→CGG) frdAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (78/73);  new base C (5/6);  total (83/79)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

Only 100 of 167 total aligned reads displayed.

AGTGTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAT  >  NC_000913/4381250‑4381543
                                                                                                                                                  |                                                                                                                                                   
 gtgtGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACgcggcg                                                                                                                                                  >  3:88542/1‑149 (MQ=255)
        gTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGAt                                                                                                                                           <  2:14581/149‑1 (MQ=255)
         tCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          <  3:857290/149‑1 (MQ=255)
            gACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGATgtgg                                                                                                                                       <  1:622538/149‑4 (MQ=255)
               gTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTg                                                                                                                                    >  2:552681/1‑149 (MQ=255)
                    ggAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGAt                                                                                                                                           <  2:459351/137‑1 (MQ=255)
                      aaTCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTAc                                                                                                                             <  1:324352/149‑1 (MQ=255)
                       aTCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACg                                                                                                                            <  2:60511/149‑1 (MQ=255)
                        tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATgg                                                                                                                                         <  2:1403044/135‑1 (MQ=255)
                        tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATgg                                                                                                                                         >  1:1403044/1‑135 (MQ=255)
                        tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGc                                                                                                                           >  3:981614/1‑149 (MQ=255)
                        tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGc                                                                                                                           <  3:942747/149‑1 (MQ=255)
                          ggTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGATgt                                                                                                                                         >  3:1215207/1‑132 (MQ=255)
                          ggTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCa                                                                                                                         >  2:1315544/1‑149 (MQ=255)
                           gTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                                        >  1:574630/1‑149 (MQ=255)
                           gTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                                        >  4:527845/1‑149 (MQ=255)
                           gTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                                        <  1:868121/149‑1 (MQ=255)
                           gTTCTGTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                                        >  4:206929/1‑149 (MQ=255)
                            ttCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGga                                                                                                                                              <  4:590094/126‑1 (MQ=255)
                            ttCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          >  3:586516/1‑130 (MQ=255)
                               tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGgcga                                                                                                                                            >  4:728090/1‑125 (MQ=255)
                               tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                                    >  3:37763/1‑149 (MQ=255)
                               tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                                    >  3:568812/1‑149 (MQ=255)
                                 tAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGgtgt                                                                                                                                      <  2:409249/129‑1 (MQ=255)
                                 tAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGc                                                                                                                  <  1:1126688/149‑1 (MQ=255)
                                 tAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGc                                                                                                                  <  2:943400/149‑1 (MQ=255)
                                  aaCCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          <  2:848989/124‑1 (MQ=255)
                                  aaCCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          >  1:848989/1‑124 (MQ=255)
                                    ccGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGtt                                                                                                                                     <  1:708605/127‑1 (MQ=255)
                                      ggATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGaa                                                                                                             >  1:38606/1‑149 (MQ=255)
                                       gATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAg                                                                                                            <  2:38606/149‑1 (MQ=255)
                                        aTCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGtt                                                                                                                                     <  2:1277745/123‑1 (MQ=255)
                                        aTCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGtt                                                                                                                                     >  1:1277745/1‑123 (MQ=255)
                                        aTCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGACGGTGTTGCCTTTACGCCATTCGTGCCAGAAgg                                                                                                           >  2:899373/1‑149 (MQ=255)
                                          cGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGcc                                                                                                         <  3:206929/149‑1 (MQ=255)
                                           gACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCt                                                                                                        >  1:534007/1‑149 (MQ=255)
                                             cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCtt                                                                                                                                <  1:1000689/123‑1 (MQ=255)
                                             cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCtt                                                                                                                                >  2:1000689/1‑123 (MQ=255)
                                              gCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTg                                                                                                                                                <  4:574178/106‑1 (MQ=255)
                                              gCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGtt                                                                                                                                     >  2:1324706/1‑117 (MQ=255)
                                                    gTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACtt                                                                                               >  1:129292/1‑149 (MQ=255)
                                                     tACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACttt                                                                                              <  1:899373/149‑1 (MQ=255)
                                                     tACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACttt                                                                                              <  4:88542/149‑1 (MQ=255)
                                                      aCGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTg                                                                                             >  4:1453950/1‑149 (MQ=255)
                                                      aCGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTg                                                                                             >  2:76638/1‑149 (MQ=255)
                                                          tttCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTcgc                                                                                         >  4:1398510/1‑149 (MQ=255)
                                                             cGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTg                                                                                      <  3:1453950/149‑1 (MQ=255)
                                                                     cGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGt                                                                              <  1:642675/149‑1 (MQ=255)
                                                                       cAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTc                                                                            <  4:755465/149‑1 (MQ=255)
                                                                        aGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTcc                                                                           <  4:981614/149‑1 (MQ=255)
                                                                        aGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTcc                                                                           >  3:510401/1‑149 (MQ=255)
                                                                         gATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGt                                                                                                                                                 >  3:779606/1‑78 (MQ=255)
                                                                         gATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGt                                                                                                                                                 <  4:779606/78‑1 (MQ=255)
                                                                         gATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgagaga                                                                                                  <  3:531521/125‑1 (MQ=255)
                                                                         gATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgagaga                                                                                                  >  4:531521/1‑125 (MQ=255)
                                                                         gATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGAGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGTTGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGCCCCAGTTCCa                                                                          <  4:949561/149‑1 (MQ=255)
                                                                          aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                                    >  2:346539/1‑106 (MQ=255)
                                                                          aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTc                                                                            >  1:1330697/1‑146 (MQ=255)
                                                                          aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTc                                                                            <  2:1330697/146‑1 (MQ=255)
                                                                          aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCat                                                                         <  1:76638/149‑1 (MQ=255)
                                                                             aaCGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCatata                                                                      <  3:1398510/149‑1 (MQ=255)
                                                                                  cAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          <  1:1045028/76‑1 (MQ=255)
                                                                                  cAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          <  2:1182389/76‑1 (MQ=255)
                                                                                  cAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATg                                                                                                                                          >  2:1045028/1‑76 (MQ=255)
                                                                                    gACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGga                                                                                                                                              <  4:170879/70‑1 (MQ=255)
                                                                                    gACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGttt                                                               <  3:216094/149‑1 (MQ=255)
                                                                                         tCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCgg                                                          <  2:534007/149‑1 (MQ=255)
                                                                                            tGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                                    >  2:663068/1‑88 (MQ=255)
                                                                                            tGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                                    <  1:663068/88‑1 (MQ=255)
                                                                                             gCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTg                                                                                      <  3:874665/117‑1 (MQ=255)
                                                                                                 ttttttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGAt                                                                                                                                           <  1:230817/60‑1 (MQ=255)
                                                                                                 ttttttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCCGAAGGCCTGAGAGACTTTGTCGCGTGGACCCCGTTCCATATATTTGTTTTTCGGCTCGCCCa                                                  >  4:1361714/1‑149 (MQ=255)
                                                                                                        tcGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGtt                                                                             <  4:860602/115‑1 (MQ=255)
                                                                                                             gAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCata                                                                        >  3:1447007/1‑115 (MQ=255)
                                                                                                             gAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCata                                                                        <  4:1447007/115‑1 (MQ=255)
                                                                                                                     gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTcc                                                                           <  2:705926/104‑1 (MQ=255)
                                                                                                                     gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTcc                                                                           >  1:705926/1‑104 (MQ=255)
                                                                                                                     gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCa                                                                          >  1:1323445/1‑105 (MQ=255)
                                                                                                                       aaGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTc                                                            <  2:215820/117‑1 (MQ=255)
                                                                                                                       aaGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTc                                                            >  1:215820/1‑117 (MQ=255)
                                                                                                                       aaGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGcc                            <  2:246877/149‑1 (MQ=255)
                                                                                                                       aaGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGcc                            <  2:646209/149‑1 (MQ=255)
                                                                                                                         gTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGt                                                                                       >  2:718744/1‑88 (MQ=255)
                                                                                                                            gagaTTAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGc                             >  4:1029599/1‑143 (MQ=255)
                                                                                                                            gagaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGc                             >  4:60819/1‑143 (MQ=255)
                                                                                                                            gagaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGc                             <  3:60819/143‑1 (MQ=255)
                                                                                                                              gaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGc                                                                                                                  >  3:65037/1‑56 (MQ=255)
                                                                                                                              gaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCt                     >  3:76871/1‑149 (MQ=255)
                                                                                                                                 aaaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc                  >  2:237808/1‑149 (MQ=255)
                                                                                                                                  aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCGTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCa                 <  4:37763/149‑1 (MQ=255)
                                                                                                                                  aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTc                                        >  1:176404/1‑126 (MQ=255)
                                                                                                                                  aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTc                                        <  2:176404/126‑1 (MQ=255)
                                                                                                                                   aCCACATCGCCACGCGGCGTGGAGATGGAGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACt                                                                                                <  1:423819/69‑1 (MQ=255)
                                                                                                                                    ccACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGa               >  3:480013/1‑149 (MQ=255)
                                                                                                                                         tCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACg          >  1:461043/1‑149 (MQ=255)
                                                                                                                                          cGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGt                                                                                            <  3:745520/66‑1 (MQ=255)
                                                                                                                                             cACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCa                 <  3:479524/138‑1 (MQ=255)
                                                                                                                                             cACGCCGCGTGGCGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTa       <  2:1090854/148‑1 (MQ=255)
                                                                                                                                                 cggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAt  <  4:925626/149‑1 (MQ=255)
                                                                                                                                                  ggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCg                                               <  1:35069/103‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGTGTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAT  >  NC_000913/4381250‑4381543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: