Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 397,277 C→G 5.5% I213M (ATC→ATG sbmA → peptide antibiotic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913397,2770CG 5.5% 604.0 / 20.3 166I213M (ATC→ATGsbmApeptide antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base C (78/78);  new base G (5/4);  total (84/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 184 total aligned reads displayed.

TACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGA  >  NC_000913/397139‑397425
                                                                                                                                          |                                                                                                                                                    
tACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGa                                                                                                                                            <  3:150267/149‑1 (MQ=255)
  cAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATc                                                                                                                                          <  1:1057601/149‑1 (MQ=255)
       aTGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCtt                                                                                                                                     >  4:248704/1‑149 (MQ=255)
        tGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTc                                                                                                                                    <  3:527001/149‑1 (MQ=255)
         gAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTACAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTcc                                                                                                                                   >  1:32484/1‑149 (MQ=255)
           aCGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTg                                                                                                                                 >  3:643260/1‑149 (MQ=255)
             gAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGcc                                                                                                                               >  1:153310/1‑149 (MQ=255)
               atatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCgg                                                                                                                             >  1:56507/1‑149 (MQ=255)
               atatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCCTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCgg                                                                                                                             <  3:842259/149‑1 (MQ=255)
                tatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGt                                                                                                                            <  2:56507/149‑1 (MQ=255)
                     cATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTgg                                                                                                                       <  4:643260/149‑1 (MQ=255)
                      aTGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGt                                                                                                                      <  2:753704/149‑1 (MQ=255)
                        ggCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                    <  1:382425/149‑1 (MQ=255)
                        ggCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                    <  4:799237/149‑1 (MQ=255)
                           gAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGc                                                                                                                 >  3:82689/1‑149 (MQ=255)
                                   aacaacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATc                                                                                                                                          <  1:765252/116‑1 (MQ=255)
                                      aacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGAt                                                                                                                                           >  1:786779/1‑112 (MQ=255)
                                      aacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                    <  2:683818/135‑1 (MQ=255)
                                      aacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                    >  1:683818/1‑135 (MQ=255)
                                              cATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGa                                                                                              >  4:1150096/1‑149 (MQ=255)
                                                tatCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGc                                                                                            >  2:763708/1‑149 (MQ=255)
                                                 atCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCt                                                                                           >  2:447201/1‑149 (MQ=255)
                                                  tCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGc                                                                                                                                <  1:777348/111‑1 (MQ=255)
                                                    gAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGt                                                                                                                                               >  3:798368/1‑94 (MQ=255)
                                                     aaGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCg                                                                                       >  2:989671/1‑149 (MQ=255)
                                                        gggCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGAtt                                                                                    >  4:1392719/1‑149 (MQ=255)
                                                          gCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatc                                                                                                                                                      >  1:192388/1‑81 (MQ=255)
                                                          gCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatc                                                                                                                                                      <  2:402988/81‑1 (MQ=255)
                                                          gCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatc                                                                                                                                                      >  1:402988/1‑81 (MQ=255)
                                                             gCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCgg                                                                               <  1:447201/149‑1 (MQ=255)
                                                                        gCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGa                                                                                      <  3:303976/131‑1 (MQ=255)
                                                                        gCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGa                                                                                      >  4:303976/1‑131 (MQ=255)
                                                                         cAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                   <  3:1260977/149‑1 (MQ=255)
                                                                         cAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                   <  2:767734/149‑1 (MQ=255)
                                                                          aGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTAt                                                                  >  3:617952/1‑149 (MQ=255)
                                                                            gAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCAt                                                                                                      <  2:296898/111‑1 (MQ=255)
                                                                            gAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCAt                                                                                                      >  1:296898/1‑111 (MQ=255)
                                                                               ggcacCATGCGTTTTGCTTCAACGCTGGCGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCt                                                             >  1:74656/3‑149 (MQ=255)
                                                                               gACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCt                                                             <  4:755980/149‑1 (MQ=255)
                                                                                acacCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTg                                                            <  3:1392719/149‑1 (MQ=255)
                                                                                acacCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTg                                                            <  4:82689/149‑1 (MQ=255)
                                                                                  acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTcc                                                                                                            >  1:588710/1‑99 (MQ=255)
                                                                                  acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTcc                                                                                                            <  2:588710/99‑1 (MQ=255)
                                                                                  acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCt                                                                                           >  3:132401/1‑116 (MQ=255)
                                                                                  acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                          <  2:635903/149‑1 (MQ=255)
                                                                                   ccATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCGCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTg                                                         >  4:1217843/1‑149 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatc                                                                                                                                                      >  3:358684/1‑54 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCcatc                                                                                                                                                      <  4:358684/54‑1 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGc                                                                                                  >  1:646862/1‑106 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAg                                                                                             >  4:1377125/1‑111 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGt                                                               >  2:783082/1‑141 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGAt                                                       <  1:989671/149‑1 (MQ=255)
                                                                                      tGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGAtt                                                      <  3:1150096/149‑1 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                   <  4:1408560/126‑1 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCg                                            >  4:330463/1‑149 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCg                                            >  3:1092137/1‑149 (MQ=255)
                                                                                                  cAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTc                                          <  4:500327/149‑1 (MQ=255)
                                                                                                            gaATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGa                                <  3:330463/149‑1 (MQ=255)
                                                                                                               tatGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgg                             >  2:159872/1‑149 (MQ=255)
                                                                                                               tatGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgg                             >  3:102737/1‑149 (MQ=255)
                                                                                                                  gggCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGa                                >  4:1458879/1‑143 (MQ=255)
                                                                                                                      gTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATg                              >  3:1408731/1‑141 (MQ=255)
                                                                                                                      gTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCgg                      >  3:465494/1‑149 (MQ=255)
                                                                                                                         aGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGt                                                               <  1:989859/105‑1 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                          >  1:760028/1‑108 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                          <  2:760028/108‑1 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc                 <  3:201914/149‑1 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc                 >  1:532636/1‑149 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc                 >  1:832768/1‑149 (MQ=255)
                                                                                                                            tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTg                                 >  4:1177658/1‑132 (MQ=255)
                                                                                                                            tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                <  4:102737/149‑1 (MQ=255)
                                                                                                                            tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                >  4:305340/1‑149 (MQ=255)
                                                                                                                            tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCCTCTGGTCGCTg                                 <  3:1177658/132‑1 (MQ=255)
                                                                                                                              tATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg              <  3:305340/149‑1 (MQ=255)
                                                                                                                              gaTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCaa                                               >  2:1267446/2‑116 (MQ=255)
                                                                                                                              gaTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCaa                                               <  1:1267446/115‑1 (MQ=255)
                                                                                                                              gaTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCt                                         >  2:745419/2‑122 (MQ=255)
                                                                                                                              gaTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCt                                         <  1:745419/121‑1 (MQ=255)
                                                                                                                                tCAACGCCATCATGACGTTGATCGTCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgggg                           <  4:914036/134‑1 (MQ=255)
                                                                                                                                tCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCTGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgggg                           >  3:914036/1‑134 (MQ=255)
                                                                                                                                 cAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg              <  3:1401340/146‑1 (MQ=255)
                                                                                                                                  aaCGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGa                                                        >  1:1436221/1‑103 (MQ=255)
                                                                                                                                  aaCGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGa                                                        <  2:1436221/103‑1 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                   <  3:678537/92‑1 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                   >  4:678537/1‑92 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                >  3:287519/1‑143 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                <  4:287519/143‑1 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGc                                                    >  1:1110678/1‑104 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGc                                                    <  2:1110678/104‑1 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTc                                          <  2:357994/114‑1 (MQ=255)
                                                                                                                                      ccATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCAtgt                                                                                                    <  4:1078919/55‑1 (MQ=255)
                                                                                                                                      ccATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTa      >  2:705091/1‑149 (MQ=255)
                                                                                                                                       catcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                          <  4:1274143/96‑1 (MQ=255)
                                                                                                                                       catcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAg     <  1:705091/149‑1 (MQ=255)
                                                                                                                                       catcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGAACCGGATTGCTGGCAGTGGTAg     >  1:1064457/1‑149 (MQ=255)
                                                                                                                                        atcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAgg    <  2:30269/149‑1 (MQ=255)
                                                                                                                                         tcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg              >  2:806154/1‑138 (MQ=255)
                                                                                                                                         tcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAggg   <  4:465494/149‑1 (MQ=255)
                                                                                                                                         tcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAggg   >  3:1274277/1‑149 (MQ=255)
                                                                                                                                          catGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGa  <  4:617952/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
TACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGA  >  NC_000913/397139‑397425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: