Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,515,968 C→G 5.9% G237A (GGA→GCA)  fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,515,9680CG 5.9% 715.7 / 27.2 205G237A (GGA→GCA) fecATonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (97/95);  new base G (7/5);  total (104/101)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 213 total aligned reads displayed.

TGTGCCGCCCACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAG  >  NC_000913/4515820‑4516113
                                                                                                                                                    |                                                                                                                                                 
tgtgCCGCCCACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGGTCTGCGGTc                                                                                                                                                   <  1:1255533/149‑1 (MQ=255)
tgtgCCGCCCACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTc                                                                                                                                                   <  2:21743/149‑1 (MQ=255)
   gccgccCACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTcc                                                                                                                                                  <  3:473605/147‑1 (MQ=255)
   gccgccCACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGt                                                                                                                                                <  4:1009431/149‑1 (MQ=255)
          accaTCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcac                                                                                                                                         >  1:449217/1‑149 (MQ=255)
              tCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccgca                                                                                                                                     >  1:139565/1‑149 (MQ=255)
              tCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccgca                                                                                                                                     >  3:1223910/1‑149 (MQ=255)
               cAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccgcac                                                                                                                                    <  1:1229639/149‑1 (MQ=255)
                aGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACgcaccgcacc                                                                                                                                   >  2:697609/1‑149 (MQ=255)
                 ggTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGcacca                                                                                                                                  >  2:93421/1‑149 (MQ=255)
                  gTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGcaccac                                                                                                                                 >  2:1180605/1‑149 (MQ=255)
                  gTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGcaccac                                                                                                                                 >  2:539655/1‑149 (MQ=255)
                          tctcTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccg                                                                                                                                       >  2:875831/1‑135 (MQ=255)
                          tctcTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccg                                                                                                                                       <  1:875831/135‑1 (MQ=255)
                              tttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCa                                                                                                                     >  1:184612/1‑149 (MQ=255)
                              tttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCa                                                                                                                     >  4:325822/1‑149 (MQ=255)
                               ttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCAc                                                                                                                    <  2:1037508/149‑1 (MQ=255)
                                 cGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccgcac                                                                                                                                    <  4:755952/131‑1 (MQ=255)
                                   ggTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCa                                                                                                                <  1:539655/149‑1 (MQ=255)
                                   ggTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCa                                                                                                                >  3:1347405/1‑149 (MQ=255)
                                    gttgttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTGCGTTAcggaccgcaga                                                                                                                                   <  1:63557/129‑3 (MQ=255)
                                    gttgttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTGCGTTAcggaccgcaga                                                                                                                                   >  2:63557/1‑127 (MQ=255)
                                       gttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATgg                                                                                                            >  4:142926/1‑149 (MQ=255)
                                        ttttGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGc                                                                                                           >  3:1102131/1‑149 (MQ=255)
                                          ttGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCAt                                                                                                         >  2:1267319/1‑149 (MQ=255)
                                          ttGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCACACCACCACCGCGTACCACGTCAATGGCAt                                                                                                         >  3:1196145/1‑149 (MQ=255)
                                            gtgAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACgcaccacacc                                                                                                                                   <  4:777688/121‑1 (MQ=255)
                                            gtgAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATcc                                                                                                       >  3:50732/1‑149 (MQ=255)
                                             tgAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAGGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCa                                                                                                      >  3:295354/1‑149 (MQ=255)
                                              gAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATg                                                                                                             <  2:980723/141‑1 (MQ=255)
                                              gAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCAt                                                                                                     <  1:105775/149‑1 (MQ=255)
                                                    gTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTcc                                                                                                                                                  >  1:207204/1‑98 (MQ=255)
                                                    gTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGcc                                                                                               >  2:987318/1‑149 (MQ=255)
                                                        ggCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGc                                                                                           <  1:1267319/149‑1 (MQ=255)
                                                          ctgagctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACgcaccgcacc                                                                                                                                   >  4:1306802/1‑107 (MQ=255)
                                                          ctgagctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACgcaccgcacc                                                                                                                                   <  3:1306802/107‑1 (MQ=255)
                                                              gctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACg                                                                                     <  2:139565/149‑1 (MQ=255)
                                                                  aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAAc                                                                                      <  1:905853/144‑1 (MQ=255)
                                                                  aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAg                                                                                 >  4:291764/1‑149 (MQ=255)
                                                                  aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAg                                                                                 >  4:1367997/1‑149 (MQ=255)
                                                                     ttCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCa                                                                              <  4:1196145/149‑1 (MQ=255)
                                                                       ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                            <  3:390113/149‑1 (MQ=255)
                                                                       ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                            <  2:883679/149‑1 (MQ=255)
                                                                       ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                            <  1:697609/149‑1 (MQ=255)
                                                                         aCGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGa                                                                          <  3:1367997/149‑1 (MQ=255)
                                                                         aCGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATAGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGa                                                                          >  4:1108743/1‑149 (MQ=255)
                                                                           gcccgccTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaaa                                                                        <  4:1347405/149‑1 (MQ=255)
                                                                           gcccgccTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaaa                                                                        <  3:1057159/149‑1 (MQ=255)
                                                                             ccgccTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCg                                                                                                                                                 >  3:726181/1‑74 (MQ=255)
                                                                                 cTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGc                                                                                                           <  1:787999/108‑1 (MQ=255)
                                                                                  tCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTAcc                                                                                                                      <  2:196218/96‑1 (MQ=255)
                                                                                  tCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATcc                                                                                                       >  2:466324/1‑111 (MQ=255)
                                                                                    gATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCa                                                                                                                     <  3:930770/95‑1 (MQ=255)
                                                                                         cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTTCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaaa                                                                        <  2:751664/135‑1 (MQ=255)
                                                                                         cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTc                                                                                                                 <  4:214985/94‑1 (MQ=255)
                                                                                         cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCaa                                                                                                               <  1:983603/96‑1 (MQ=255)
                                                                                         cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACAGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaac                                                                        >  1:751664/1‑134 (MQ=255)
                                                                                          aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGt                                                         >  1:345794/1‑149 (MQ=255)
                                                                                            aGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTaa                                                       <  4:1223910/149‑1 (MQ=255)
                                                                                              tCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                             <  3:664424/109‑1 (MQ=255)
                                                                                               ccTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAgg                                                     <  1:479199/148‑1 (MQ=255)
                                                                                               ccTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAgg                                                     >  2:479199/1‑148 (MQ=255)
                                                                                                cTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAgggg                                                   <  3:142926/149‑1 (MQ=255)
                                                                                                cTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAgggg                                                   >  1:1424893/1‑149 (MQ=255)
                                                                                                          gCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTcc                                                                                                                                                  <  4:84287/44‑1 (MQ=255)
                                                                                                          gCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTcc                                                                                                                                                  >  3:84287/1‑44 (MQ=255)
                                                                                                            aCGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                             >  4:49292/1‑143 (MQ=255)
                                                                                                                gTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGt                                                                                                   >  1:154681/1‑85 (MQ=255)
                                                                                                                 tAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACggcatcc                                  <  2:345794/149‑6 (MQ=255)
                                                                                                                 tAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACggcatcc                                  <  4:1088828/149‑6 (MQ=255)
                                                                                                                 gaaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACggcatcc                                  <  3:28375/148‑6 (MQ=255)
                                                                                                                  aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACgg                                                                                    <  3:768420/98‑1 (MQ=255)
                                                                                                                  aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGc                                                  <  4:1240333/132‑1 (MQ=255)
                                                                                                                  aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGc                                                  >  3:1240333/1‑132 (MQ=255)
                                                                                                                  aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGAt                                   >  4:63601/1‑147 (MQ=255)
                                                                                                                  aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGc                                 >  3:20910/1‑149 (MQ=255)
                                                                                                                   aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGc                                                  <  1:938825/131‑1 (MQ=255)
                                                                                                                   aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                             >  4:453742/1‑136 (MQ=255)
                                                                                                                   aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                             <  1:281891/136‑1 (MQ=255)
                                                                                                                   aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACgg                                       <  2:140666/142‑1 (MQ=255)
                                                                                                                   aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGcc                                >  3:568762/1‑149 (MQ=255)
                                                                                                                     aaaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGAc                                                            >  1:391807/1‑119 (MQ=255)
                                                                                                                     aaaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGAc                                                            <  2:391807/119‑1 (MQ=255)
                                                                                                                     aaaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGt                              <  4:568762/149‑1 (MQ=255)
                                                                                                                      aaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACgg                                                                                    >  1:1140476/1‑94 (MQ=255)
                                                                                                                       aGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTcc                            <  2:16173/149‑1 (MQ=255)
                                                                                                                           caccacGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCa                        <  1:862218/149‑1 (MQ=255)
                                                                                                                                    gccCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCg               >  2:826482/1‑149 (MQ=255)
                                                                                                                                      ccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                             >  4:709851/1‑117 (MQ=255)
                                                                                                                                      ccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                             >  4:887726/1‑117 (MQ=255)
                                                                                                                                      ccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                             <  3:887726/117‑1 (MQ=255)
                                                                                                                                       cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTaa                                                       >  2:285447/1‑106 (MQ=255)
                                                                                                                                          gCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCAATCAGGACGGTCGAGCGGc         >  4:172761/1‑149 (MQ=255)
                                                                                                                                            tctGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACgg                                       <  4:233456/117‑1 (MQ=255)
                                                                                                                                             gtGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                             >  3:215250/2‑62 (MQ=37)
                                                                                                                                              tGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACg                                        >  2:1107900/1‑114 (MQ=255)
                                                                                                                                              tGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACg                                        <  1:1107900/114‑1 (MQ=255)
                                                                                                                                              tGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACgg                                       >  2:142769/1‑115 (MQ=255)
                                                                                                                                                cGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATcc                                                                                                       <  1:297661/49‑1 (MQ=255)
                                                                                                                                                 ggTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAg  >  2:1128301/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGTGCCGCCCACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAG  >  NC_000913/4515820‑4516113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: