Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,515,972 A→T 6.2% Y236N (TAC→AAC)  fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,515,9720AT 6.2% 746.5 / 29.7 208Y236N (TAC→AAC) fecATonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base A (98/97);  new base T (7/6);  total (105/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

Only 100 of 209 total aligned reads displayed.

CACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGG  >  NC_000913/4515829‑4516120
                                                                                                                                               |                                                                                                                                                    
 accaTCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcac                                                                                                                                                >  1:449217/1‑149 (MQ=255)
     tCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAc                                                                                                                                                    <  3:905682/141‑1 (MQ=255)
      cAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccgcac                                                                                                                                           <  1:1229639/149‑1 (MQ=255)
        ggTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGcacca                                                                                                                                         >  2:93421/1‑149 (MQ=255)
         gTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGcaccac                                                                                                                                        >  2:539655/1‑149 (MQ=255)
         gTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGcaccac                                                                                                                                        >  2:1180605/1‑149 (MQ=255)
                 tctcTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccg                                                                                                                                              >  2:875831/1‑135 (MQ=255)
                   tctTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTAc                                                                                                                              >  3:546737/1‑149 (MQ=255)
                     tttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCa                                                                                                                            >  4:325822/1‑149 (MQ=255)
                     tttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCa                                                                                                                            >  1:184612/1‑149 (MQ=255)
                     tttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCa                                                                                                                            >  2:740236/1‑149 (MQ=255)
                      ttCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCAc                                                                                                                           <  2:1037508/149‑1 (MQ=255)
                        cGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAcgcaccgcac                                                                                                                                           >  3:755952/1‑131 (MQ=255)
                          ggTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCa                                                                                                                       <  1:539655/149‑1 (MQ=255)
                           gttgttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTGCGTTAcggaccgcaga                                                                                                                                          <  1:63557/129‑3 (MQ=255)
                           gttgttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTGCGTTAcggaccgcaga                                                                                                                                          >  2:63557/1‑127 (MQ=255)
                            ttgttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAAt                                                                                                                     <  1:237653/149‑1 (MQ=255)
                            ttgttTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATAGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAAt                                                                                                                     <  2:75262/149‑1 (MQ=255)
                               ttttGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGc                                                                                                                  >  3:883526/1‑149 (MQ=255)
                                   gtgAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACgcaccacacc                                                                                                                                          >  3:777688/1‑121 (MQ=255)
                                    tgAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAGGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCa                                                                                                             >  3:295354/1‑149 (MQ=255)
                                     gAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATg                                                                                                                    <  2:980723/141‑1 (MQ=255)
                                     gAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCAt                                                                                                            <  1:105775/149‑1 (MQ=255)
                                           gTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGcc                                                                                                      >  2:987318/1‑149 (MQ=255)
                                             tGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                                    <  2:449217/149‑1 (MQ=255)
                                                gctgagctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCg                                                                                                 >  1:468187/1‑149 (MQ=255)
                                                 ctgagctgaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACgcaccgcacc                                                                                                                                          >  4:1306802/1‑107 (MQ=255)
                                                        gaCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGa                                                                                         <  4:50732/149‑1 (MQ=255)
                                                         aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAc                                                                                                                                                    >  4:1442642/1‑89 (MQ=255)
                                                         aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAAc                                                                                                                                                    <  3:1442642/89‑1 (MQ=255)
                                                         aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAAc                                                                                             <  1:905853/144‑1 (MQ=255)
                                                         aCCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAg                                                                                        >  4:1367997/1‑149 (MQ=255)
                                                            ttCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCa                                                                                     <  2:468187/149‑1 (MQ=255)
                                                            ttCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCa                                                                                     <  4:1196145/149‑1 (MQ=255)
                                                              ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                                   <  3:390113/149‑1 (MQ=255)
                                                              ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                                   <  2:883679/149‑1 (MQ=255)
                                                              ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                                   <  1:697609/149‑1 (MQ=255)
                                                              ccACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGt                                                                                   <  3:1313400/149‑1 (MQ=255)
                                                                aCGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATAGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGa                                                                                 >  4:1108743/1‑149 (MQ=255)
                                                                  gcccgccTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaaa                                                                               <  4:1347405/149‑1 (MQ=255)
                                                                  gcccgccTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaaa                                                                               <  3:1057159/149‑1 (MQ=255)
                                                                        cTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGc                                                                                                                  <  1:787999/108‑1 (MQ=255)
                                                                         tCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTAcc                                                                                                                             <  2:196218/96‑1 (MQ=255)
                                                                                cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTc                                                                                                                        <  4:214985/94‑1 (MQ=255)
                                                                                cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCaa                                                                                                                      <  1:983603/96‑1 (MQ=255)
                                                                                cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                                    <  4:1071522/114‑1 (MQ=255)
                                                                                cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACAGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaac                                                                               >  1:751664/1‑134 (MQ=255)
                                                                                cAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCATAACGCACCGCACCACCACCGCATACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGaaa                                                                               <  2:297813/135‑1 (MQ=255)
                                                                                 aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAAc                                                                                             >  3:1354572/1‑120 (MQ=255)
                                                                                 aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCt                                                                      <  2:1291124/143‑1 (MQ=255)
                                                                                 aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCt                                                                      >  1:1291124/1‑143 (MQ=255)
                                                                                 aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGt                                                                >  1:345794/1‑149 (MQ=255)
                                                                                 aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGt                                                                >  3:1088828/1‑149 (MQ=255)
                                                                                 aaaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGt                                                                >  4:28375/1‑149 (MQ=255)
                                                                                  aaGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTa                                                               >  4:203552/1‑149 (MQ=255)
                                                                                   aGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTaa                                                              <  4:1223910/149‑1 (MQ=255)
                                                                                     tCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                                    <  3:664424/109‑1 (MQ=255)
                                                                                              aTGGCACGGGTAACAAGGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAggg                                                   <  4:295354/149‑1 (MQ=255)
                                                                                                   aCGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                                    <  3:49292/143‑1 (MQ=255)
                                                                                                       gTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGt                                                                                                          >  1:154681/1‑85 (MQ=255)
                                                                                                       gTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGAt                                          <  3:1274785/149‑1 (MQ=255)
                                                                                                        tAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATg                                         >  2:1397471/1‑149 (MQ=255)
                                                                                                        gaaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACggcatcc                                         <  3:28375/148‑6 (MQ=255)
                                                                                                         aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACgg                                                                                           >  4:768420/1‑98 (MQ=255)
                                                                                                         aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGc                                                         <  4:1240333/132‑1 (MQ=255)
                                                                                                         aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGAt                                          >  4:63601/1‑147 (MQ=255)
                                                                                                         aaCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGc                                        >  3:20910/1‑149 (MQ=255)
                                                                                                          aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGc                                                         <  1:938825/131‑1 (MQ=255)
                                                                                                          aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                                    >  4:453742/1‑136 (MQ=255)
                                                                                                          aCAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACgg                                              >  1:140666/1‑142 (MQ=255)
                                                                                                            aaaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGAc                                                                   >  1:391807/1‑119 (MQ=255)
                                                                                                            aaaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGt                                     <  4:568762/149‑1 (MQ=255)
                                                                                                             aaGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACgg                                                                                           >  1:1140476/1‑94 (MQ=255)
                                                                                                              aGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTcc                                   <  2:16173/149‑1 (MQ=255)
                                                                                                                  caccacGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGTGAGCCAGTGAAAGCTGCGGCTGACCGTAAgg                                                            >  4:1007152/1‑120 (MQ=255)
                                                                                                                  caccacGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCa                               <  1:862218/149‑1 (MQ=255)
                                                                                                                     cacGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGa                                                                                         <  1:1265156/88‑1 (MQ=255)
                                                                                                                     cacGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGa                                                                                         >  2:1265156/1‑88 (MQ=255)
                                                                                                                           gccCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCg                      >  2:826482/1‑149 (MQ=255)
                                                                                                                            cccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGa                     <  3:592619/149‑1 (MQ=255)
                                                                                                                             ccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                                    >  4:887726/1‑117 (MQ=255)
                                                                                                                             ccACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAgg                                                    >  4:709851/1‑117 (MQ=255)
                                                                                                                              cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTaa                                                              <  1:285447/106‑1 (MQ=255)
                                                                                                                              cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTaa                                                              >  2:285447/1‑106 (MQ=255)
                                                                                                                              cACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGc                   <  3:1235366/149‑1 (MQ=255)
                                                                                                                                   tctGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTTAAAGCTGCgg                                                                        <  3:1401968/91‑1 (MQ=255)
                                                                                                                                   tctGCGGTCCGTAACCCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCgg                                                                        >  4:1401968/1‑91 (MQ=255)
                                                                                                                                    gtGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                                    >  3:1209883/2‑62 (MQ=37)
                                                                                                                                    gtGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGa                                                                                                    >  3:215250/2‑62 (MQ=37)
                                                                                                                                    ctGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTgg             <  2:366989/149‑1 (MQ=255)
                                                                                                                                     tGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACg                                               <  1:1107900/114‑1 (MQ=255)
                                                                                                                                     tGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACg                                               >  2:1107900/1‑114 (MQ=255)
                                                                                                                                     tGCGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTcc                                   >  1:87763/1‑126 (MQ=255)
                                                                                                                                     tGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACgg                                              >  2:142769/1‑115 (MQ=255)
                                                                                                                                     tGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACgg                                              <  1:142769/115‑1 (MQ=255)
                                                                                                                                       cGGTGCGTTACGGACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGa          >  4:819804/1‑149 (MQ=255)
                                                                                                                                        ggTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAg         >  2:1128301/1‑149 (MQ=255)
                                                                                                                                             gTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGcgcg    >  2:487944/1‑149 (MQ=255)
                                                                                                                                              tAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCgg   <  1:487944/149‑1 (MQ=255)
                                                                                                                                               aaCGCACCGCACCACCACCGCGTACCACGTCAATAGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCggg  <  3:1108743/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CACCATCAGGTTGTGCGTCTCTTTCGGGTTGTTTTGTGAAGAGGTTGGGCTGAGCTGACCTTCCACGCCCGCCTCGATACCAAAGTCCTGCGGAATGGCACGGGTAACAAAGTTCACCACGCCGCCCACGCTCTGCGGTCCGTAACGCACCGCACCACCACCGCGTACCACGTCAATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGG  >  NC_000913/4515829‑4516120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: