Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 397,281 A→G 6.0% T215A (ACG→GCG)  sbmA → peptide antibiotic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913397,2810AG 6.0% 614.5 / 20.2 182T215A (ACG→GCG) sbmApeptide antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base A (85/86);  new base G (6/5);  total (91/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

Only 100 of 184 total aligned reads displayed.

TACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATC  >  NC_000913/397139‑397427
                                                                                                                                              |                                                                                                                                                  
tACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGa                                                                                                                                              <  4:818228/149‑1 (MQ=255)
  cAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATc                                                                                                                                            <  1:1057601/149‑1 (MQ=255)
     cGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGcc                                                                                                                                         <  3:1297389/149‑1 (MQ=255)
      gATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCt                                                                                                                                        >  2:452101/1‑149 (MQ=255)
       aTGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCtt                                                                                                                                       >  4:248704/1‑149 (MQ=255)
        tGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTc                                                                                                                                      <  3:527001/149‑1 (MQ=255)
             gAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGcc                                                                                                                                 >  1:153310/1‑149 (MQ=255)
               atatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCgg                                                                                                                               >  1:56507/1‑149 (MQ=255)
               atatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCCTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCgg                                                                                                                               <  3:842259/149‑1 (MQ=255)
                    aCATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTg                                                                                                                          >  1:755578/1‑149 (MQ=255)
                     cATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTgg                                                                                                                         <  4:643260/149‑1 (MQ=255)
                      aTGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGt                                                                                                                        <  2:753704/149‑1 (MQ=255)
                      aTGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGt                                                                                                                        <  2:153310/149‑1 (MQ=255)
                           gAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGc                                                                                                                   >  3:82689/1‑149 (MQ=255)
                               tGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGa                                                                                                                                                    <  3:291723/112‑1 (MQ=255)
                                   aacaacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATc                                                                                                                                            >  2:765252/1‑116 (MQ=255)
                                      aacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                      <  2:683818/135‑1 (MQ=255)
                                      aacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGc                                                                                                                   <  3:1202582/138‑1 (MQ=255)
                                          gCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGc                                                                                                    <  3:248704/149‑1 (MQ=255)
                                             tCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCgg                                                                                                 >  3:1150368/1‑149 (MQ=255)
                                                tatCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGc                                                                                              >  1:731138/1‑149 (MQ=255)
                                                   cGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGc                                                                                           <  4:1150368/149‑1 (MQ=255)
                                                    gAAGGGGCCGCTCAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGcc                                                                                          >  1:767734/1‑149 (MQ=255)
                                                    gAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGt                                                                                                                                                 <  4:798368/94‑1 (MQ=255)
                                                    gAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGt                                                                                                                                                 >  3:798368/1‑94 (MQ=255)
                                                     aaGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCg                                                                                         >  3:664163/1‑149 (MQ=255)
                                                     aaGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCg                                                                                         >  2:989671/1‑149 (MQ=255)
                                                        gggCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGAtt                                                                                      >  4:1392719/1‑149 (MQ=255)
                                                             gCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCgg                                                                                 <  1:763708/149‑1 (MQ=255)
                                                             gCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCgg                                                                                 <  1:447201/149‑1 (MQ=255)
                                                              cacaGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGc                                                                                                                                  <  4:874672/99‑1 (MQ=255)
                                                                   cGTGTGCAGGAAGACACCATGCGTTTTGCTACAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACAt                                                                           <  2:32484/149‑1 (MQ=255)
                                                                        gCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGa                                                                                        >  4:303976/1‑131 (MQ=255)
                                                                         cAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                     <  2:767734/149‑1 (MQ=255)
                                                                         cAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                     >  1:635903/1‑149 (MQ=255)
                                                                          aGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTAt                                                                    >  3:617952/1‑149 (MQ=255)
                                                                            gAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCAt                                                                                                        <  2:296898/111‑1 (MQ=255)
                                                                            gAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCAt                                                                                                        >  1:296898/1‑111 (MQ=255)
                                                                               ggcacCATGCGTTTTGCTTCAACGCTGGCGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCt                                                               >  1:74656/3‑149 (MQ=255)
                                                                                acacCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTg                                                              <  1:693916/149‑1 (MQ=255)
                                                                                acacCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTg                                                              <  4:664163/149‑1 (MQ=255)
                                                                                  acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                            <  3:326730/149‑1 (MQ=255)
                                                                                   ccATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCGCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTg                                                           >  4:1217843/1‑149 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGc                                                                                                    <  2:646862/106‑1 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAg                                                                                               >  4:1377125/1‑111 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAg                                                                                               <  3:1377125/111‑1 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGt                                                                 >  2:783082/1‑141 (MQ=255)
                                                                                     aTGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGt                                                                 <  1:783082/141‑1 (MQ=255)
                                                                                              gCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAAt                                                >  4:477449/1‑149 (MQ=255)
                                                                                               cTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATc                                               <  4:712698/149‑1 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                     <  4:1408560/126‑1 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                     >  3:1408560/1‑126 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATc                                               <  2:1234206/148‑1 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCg                                              >  3:1092137/1‑149 (MQ=255)
                                                                                                ttCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCg                                              >  4:330463/1‑149 (MQ=255)
                                                                                                          gagaATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGc                                                                                           >  4:942358/1‑94 (MQ=255)
                                                                                                          gagaATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTgg                                                             >  4:593872/1‑124 (MQ=255)
                                                                                                              atatGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATg                                <  2:1277490/149‑1 (MQ=255)
                                                                                                               tatGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgg                               >  3:102737/1‑149 (MQ=255)
                                                                                                                  gggCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGa                                  >  4:1458879/1‑143 (MQ=255)
                                                                                                                  gggCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGa                                  <  3:1458879/143‑1 (MQ=255)
                                                                                                                   ggCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgg                               <  2:41980/145‑1 (MQ=255)
                                                                                                                      gTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCgg                        >  3:465494/1‑149 (MQ=255)
                                                                                                                         aGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGt                                                                 <  1:989859/105‑1 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                            <  2:760028/108‑1 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc                   >  1:30269/1‑149 (MQ=255)
                                                                                                                           ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc                   >  1:532636/1‑149 (MQ=255)
                                                                                                                            tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                  >  4:305340/1‑149 (MQ=255)
                                                                                                                            tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCCTCTGGTCGCTg                                   <  3:1177658/132‑1 (MQ=255)
                                                                                                                            ccgattaaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                  <  1:933940/143‑1 (MQ=255)
                                                                                                                              tATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg                <  3:305340/149‑1 (MQ=255)
                                                                                                                              gaTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCaa                                                 <  1:1267446/115‑1 (MQ=255)
                                                                                                                              gaTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCt                                           >  2:745419/2‑122 (MQ=255)
                                                                                                                              gaTCAACGTCATAATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg                >  3:1993/2‑149 (MQ=255)
                                                                                                                                tCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCa              >  1:568970/1‑149 (MQ=255)
                                                                                                                                tCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCTGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgggg                             >  3:914036/1‑134 (MQ=255)
                                                                                                                                 cAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg                <  3:1401340/146‑1 (MQ=255)
                                                                                                                                 cAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCaa                                                 >  1:392689/1‑113 (MQ=255)
                                                                                                                                  aaCGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGa                                                          >  1:1436221/1‑103 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                     <  3:678537/92‑1 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTg                                          <  3:332343/119‑1 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt                  <  4:287519/143‑1 (MQ=255)
                                                                                                                                  aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGt            >  1:67896/1‑149 (MQ=255)
                                                                                                                                   aCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGTACATTCCGTAt                                                                    <  4:380031/92‑1 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGc                                                      >  1:1110678/1‑104 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTc                                            <  2:357994/114‑1 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTc                                            >  1:357994/1‑114 (MQ=255)
                                                                                                                                     gCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGt         <  4:1092137/149‑1 (MQ=255)
                                                                                                                                      ccATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCAtgt                                                                                                      <  4:1078919/55‑1 (MQ=255)
                                                                                                                                      ccATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTa        >  2:705091/1‑149 (MQ=255)
                                                                                                                                       catgatGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAg       <  2:568970/149‑1 (MQ=255)
                                                                                                                                       catcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                            <  4:1274143/96‑1 (MQ=255)
                                                                                                                                       catcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                            >  3:1274143/1‑96 (MQ=255)
                                                                                                                                       catcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGAACCGGATTGCTGGCAGTGGTAg       >  1:1064457/1‑149 (MQ=255)
                                                                                                                                        atcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGAACCGGATTGCTGGCAGTGGTAgg      <  2:1064457/149‑1 (MQ=255)
                                                                                                                                         tcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg                <  1:806154/138‑1 (MQ=255)
                                                                                                                                         tcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg                >  2:806154/1‑138 (MQ=255)
                                                                                                                                           atGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGAt   >  2:615112/1‑149 (MQ=255)
                                                                                                                                            tGACGTTGATTGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTAt                                                                    <  4:1201270/83‑1 (MQ=255)
                                                                                                                                             gACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGc                                                      <  3:965865/96‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGGGATC  >  NC_000913/397139‑397427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: