Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,548,400 G→A 6.6% C39Y (TGT→TAT) ‡ fimG → minor component of type 1 fimbriae

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,548,4000GA 6.6% 490.4 / 19.1 151C39Y (TGT→TAT) ‡fimGminor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base G (67/74);  new base A (5/5);  total (72/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

Only 100 of 155 total aligned reads displayed.

TTCACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTT  >  NC_000913/4548252‑4548547
                                                                                                                                                    |                                                                                                                                                   
ttCACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCgtg                                                                                                                                                     >  2:8592/1‑149 (MQ=255)
ttCACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCgtg                                                                                                                                                     <  4:713597/149‑1 (MQ=255)
ttCACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCgtg                                                                                                                                                     >  2:1196410/1‑149 (MQ=255)
       ttGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGtt                                                                                                                                              >  3:1357050/1‑149 (MQ=255)
          aaTATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                           <  1:741629/149‑1 (MQ=255)
            tatCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                         >  2:14844/1‑149 (MQ=255)
            tatCAGTAACTGGAGATGCTCATGAAATGGCGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACAATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGtt                                                                                                                                              >  2:380376/1‑144 (MQ=255)
               cAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                           >  4:559052/1‑144 (MQ=255)
               cAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                           <  3:559052/144‑1 (MQ=255)
                aGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccaccacc                                                                                                                                     >  4:450677/1‑149 (MQ=255)
                 gTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                         <  1:565464/144‑1 (MQ=255)
                 gTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGtttgg                                                                                                                                           >  1:781475/1‑140 (MQ=255)
                 gTAACTGGAGATGCTCAGGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                         >  2:565464/1‑144 (MQ=255)
                     cTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGc                                                                                                                                <  3:450677/149‑1 (MQ=255)
                       ggAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCa                                                                                                                              >  1:133005/1‑149 (MQ=255)
                       ggAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCa                                                                                                                              <  3:566751/149‑1 (MQ=255)
                          gaTGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg                                                                                                                           <  1:8592/149‑1 (MQ=255)
                          gaTGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACgg                                                                                                                           >  3:567835/1‑149 (MQ=255)
                               tCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAt                                                                                                                      <  4:1213756/149‑1 (MQ=255)
                                  tGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAAt                                                                                                                                  <  1:1028326/134‑1 (MQ=255)
                                   gAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCg                                                                                                                  >  2:1378073/1‑149 (MQ=255)
                                    aaaTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCgg                                                                                                                 <  1:1196410/149‑1 (MQ=255)
                                    aaaTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGttt                                                                                                                                             >  3:1249453/1‑121 (MQ=255)
                                      aTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTa                                                                                                                                                   <  2:1297697/113‑1 (MQ=255)
                                      aTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTa                                                                                                                                                   <  4:259411/113‑1 (MQ=255)
                                        ggTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGAt                                                                                                             <  1:145308/149‑1 (MQ=255)
                                          tGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                         <  1:171162/119‑1 (MQ=255)
                                           gCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCtt                                                                                                          >  2:910150/1‑149 (MQ=255)
                                            cAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCttt                                                                                                         >  2:747327/1‑149 (MQ=255)
                                             aaaCGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACATGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                        <  3:363966/149‑1 (MQ=255)
                                               aCGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAtt                                                                                                      <  1:910150/149‑1 (MQ=255)
                                                 gTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCt                                                                                                    <  1:619205/149‑1 (MQ=255)
                                                  tGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCtt                                                                                                   >  2:752633/1‑149 (MQ=255)
                                                   gggTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                  >  3:161451/1‑149 (MQ=255)
                                                   gggTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                  <  4:161451/149‑1 (MQ=255)
                                                    ggTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTc                                                                                                 <  4:1357050/149‑1 (MQ=255)
                                                     gtatgtatTGGCGGCAATATTGGCGCTCGCAAGTACGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                >  2:544353/1‑149 (MQ=255)
                                                        tgtatTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                        <  4:1457258/138‑1 (MQ=255)
                                                          tatTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCtt                                                                                           >  3:691504/1‑149 (MQ=255)
                                                           atTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTa                                                                                          <  4:567835/149‑1 (MQ=255)
                                                            ttGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                         >  4:685549/1‑101 (MQ=255)
                                                            ttGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                         <  3:685549/101‑1 (MQ=255)
                                                            ttGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTAt                                                                                         <  4:530322/149‑1 (MQ=255)
                                                             tGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                  >  1:528736/1‑139 (MQ=255)
                                                                 ggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTg                                                                                    >  1:498250/1‑149 (MQ=255)
                                                                   cAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACTGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGcc                                                                                  >  1:341232/1‑149 (MQ=255)
                                                                    aaTATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                           <  2:517644/91‑1 (MQ=255)
                                                                    aaTATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCg                                                                                 >  1:595129/1‑149 (MQ=255)
                                                                      tatTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCggg                                                                               <  2:595129/149‑1 (MQ=255)
                                                                                  cAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCg                                                                                                                  <  1:393388/102‑1 (MQ=255)
                                                                                   aaGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTc                                                                                                 <  4:199084/118‑1 (MQ=255)
                                                                                   aaGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTc                                                                                                 >  3:199084/1‑118 (MQ=255)
                                                                                       gCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCa                                                              >  2:416717/1‑149 (MQ=255)
                                                                                         gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATc                                                                                                            <  4:74077/101‑1 (MQ=255)
                                                                                         gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                  <  2:1292007/111‑1 (MQ=255)
                                                                                         gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                  >  1:1292007/1‑111 (MQ=255)
                                                                                         gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGggc                                                                             <  4:320097/132‑1 (MQ=255)
                                                                                         gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGggc                                                                             >  3:320097/1‑132 (MQ=255)
                                                                                         gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCt                                                                  >  1:1104618/1‑143 (MQ=255)
                                                                                              tACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCttt                                                                                                         <  2:172049/99‑1 (MQ=255)
                                                                                                cAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTgc                                                     >  4:1455526/1‑149 (MQ=255)
                                                                                                  ggCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGtttg                                                                                                                                            >  3:1193122/1‑59 (MQ=255)
                                                                                                  ggCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGtttg                                                                                                                                            <  4:1193122/60‑2 (MQ=255)
                                                                                                    cAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGTCATCGGCCTGGCATGATGTTGCGCtt                                                 <  2:708677/149‑1 (MQ=255)
                                                                                                      gCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                        >  2:1032298/1‑92 (MQ=255)
                                                                                                             tCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACt                                        >  1:1015879/1‑149 (MQ=255)
                                                                                                              cACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGtt                                                                                                                         <  1:415524/67‑1 (MQ=255)
                                                                                                              cACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGtt                                                                                                                         >  2:415524/1‑67 (MQ=255)
                                                                                                              cACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCgggg                                                                              >  2:590362/1‑110 (MQ=255)
                                                                                                              cACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTa                                       >  1:132078/1‑149 (MQ=255)
                                                                                                               aCCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                               >  4:775014/1‑140 (MQ=255)
                                                                                                               aCCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                               <  3:775014/140‑1 (MQ=255)
                                                                                                                 cATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAAtt                                    <  3:968915/149‑1 (MQ=255)
                                                                                                                   tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                <  2:259478/87‑1 (MQ=255)
                                                                                                                   tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                <  4:1049552/87‑1 (MQ=255)
                                                                                                                   tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                >  3:1049552/1‑87 (MQ=255)
                                                                                                                    cACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTc                                 <  3:216043/149‑1 (MQ=255)
                                                                                                                    cACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTc                                 <  2:1015879/149‑1 (MQ=255)
                                                                                                                       ggTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGtt                                                       <  4:1379554/124‑1 (MQ=255)
                                                                                                                          gAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAtt                                                                                                      <  3:152867/74‑1 (MQ=255)
                                                                                                                             cGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGaa                        <  1:583321/149‑1 (MQ=255)
                                                                                                                                 aaGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAt                                                                                                       <  4:974813/66‑1 (MQ=255)
                                                                                                                                 aaGGTCGTCGCCAAACCGTGTACGGTTCCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAt                                                                                                       >  3:974813/1‑66 (MQ=255)
                                                                                                                                   ggTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAAtc                                    <  2:1241153/131‑2 (MQ=255)
                                                                                                                                   ggTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGa                  <  1:747327/149‑1 (MQ=255)
                                                                                                                                     tcgtcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTgc                                                     <  1:215984/112‑1 (MQ=255)
                                                                                                                                        tcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGcc                                                                   >  3:768345/1‑95 (MQ=255)
                                                                                                                                        tcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGcc                                                                   <  4:768345/95‑1 (MQ=255)
                                                                                                                                        tcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAgg                >  3:456928/1‑146 (MQ=255)
                                                                                                                                           ggaaaCCGTACACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGAc                                         >  3:1438833/3‑118 (MQ=255)
                                                                                                                                           ccAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACt          <  3:1455526/149‑1 (MQ=255)
                                                                                                                                            gaaaCCGTACACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                               >  1:350047/2‑111 (MQ=255)
                                                                                                                                            gaaaCCGTACACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                               <  2:350047/110‑1 (MQ=255)
                                                                                                                                             aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                               >  2:176226/1‑110 (MQ=255)
                                                                                                                                             aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                               <  1:176226/110‑1 (MQ=255)
                                                                                                                                             aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGt                             >  1:512090/1‑128 (MQ=255)
                                                                                                                                             aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGt                             <  2:512090/128‑1 (MQ=255)
                                                                                                                                             aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGc        <  1:125034/149‑1 (MQ=255)
                                                                                                                                              aaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGcc       >  2:869306/1‑149 (MQ=255)
                                                                                                                                                   tgtACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGCCAGCtt  >  1:1390062/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTT  >  NC_000913/4548252‑4548547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: