Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,548,401 T→C 6.6% C39C (TGT→TGC) ‡ fimG → minor component of type 1 fimbriae

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,548,4010TC 6.6% 510.0 / 18.3 151C39C (TGT→TGC) ‡fimGminor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base T (67/74);  new base C (5/5);  total (72/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 158 total aligned reads displayed.

ACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCA  >  NC_000913/4548255‑4548549
                                                                                                                                                  |                                                                                                                                                    
aCTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACATGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTAc                                                                                                                                                    >  4:363966/1‑149 (MQ=255)
    ttGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGtt                                                                                                                                                <  3:490496/149‑1 (MQ=255)
    ttGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGtt                                                                                                                                                >  3:1357050/1‑149 (MQ=255)
    ttGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGtt                                                                                                                                                >  2:741629/1‑149 (MQ=255)
       aaTATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                             <  1:741629/149‑1 (MQ=255)
        atatCAGTACCTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGAGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcca                                                                                                                                            <  3:436737/149‑1 (MQ=255)
         tatCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTAAGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGtt                                                                                                                                                <  1:380376/144‑1 (MQ=255)
            cAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                             >  4:559052/1‑144 (MQ=255)
             aGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccaccacc                                                                                                                                       >  4:450677/1‑149 (MQ=255)
              gTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                           <  1:565464/144‑1 (MQ=255)
              gTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGtttgg                                                                                                                                             >  1:781475/1‑140 (MQ=255)
              gTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGtttgg                                                                                                                                             <  2:781475/142‑3 (MQ=255)
              gTAACTGGAGATGCTCAGGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                           >  2:565464/1‑144 (MQ=255)
                aaCTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAAt                                                                                                                                    <  3:622833/149‑1 (MQ=255)
                    ggAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCa                                                                                                                                <  3:566751/149‑1 (MQ=255)
                    ggAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCa                                                                                                                                >  1:133005/1‑149 (MQ=255)
                            tCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGAt                                                                                                                        <  4:1213756/149‑1 (MQ=255)
                               tGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAAt                                                                                                                                    >  2:1028326/1‑134 (MQ=255)
                               tGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAAt                                                                                                                                    <  1:1028326/134‑1 (MQ=255)
                                gAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCg                                                                                                                    >  2:1378073/1‑149 (MQ=255)
                                 aaaTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCgg                                                                                                                   <  1:1196410/149‑1 (MQ=255)
                                 aaaTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGttt                                                                                                                                               >  3:1249453/1‑121 (MQ=255)
                                   aTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTa                                                                                                                                                     >  3:259411/1‑113 (MQ=255)
                                   aTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTa                                                                                                                                                     <  4:259411/113‑1 (MQ=255)
                                   aTGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTa                                                                                                                                                     >  1:1297697/1‑113 (MQ=255)
                                     ggTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGAt                                                                                                               <  1:145308/149‑1 (MQ=255)
                                     ggTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGAt                                                                                                               <  1:1378073/149‑1 (MQ=255)
                                       tGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                           >  2:171162/1‑119 (MQ=255)
                                        gCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCtt                                                                                                            >  2:910150/1‑149 (MQ=255)
                                          aaaCGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                          <  1:14844/149‑1 (MQ=255)
                                            aCGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAtt                                                                                                        <  1:910150/149‑1 (MQ=255)
                                              gTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCt                                                                                                      <  1:619205/149‑1 (MQ=255)
                                               tGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCtt                                                                                                     >  2:752633/1‑149 (MQ=255)
                                                gggTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                    <  4:161451/149‑1 (MQ=255)
                                                gggTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                    >  3:161451/1‑149 (MQ=255)
                                                 ggTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTc                                                                                                   <  4:1357050/149‑1 (MQ=255)
                                                     tgtatTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                          <  4:1457258/138‑1 (MQ=255)
                                                     tgtatTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                          >  3:1457258/1‑138 (MQ=255)
                                                        atTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTa                                                                                            <  4:567835/149‑1 (MQ=255)
                                                         ttGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTccac                                                                                                                                           <  3:685549/101‑1 (MQ=255)
                                                         ttGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTAt                                                                                           <  4:530322/149‑1 (MQ=255)
                                                         ttGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTAt                                                                                           <  1:752633/149‑1 (MQ=255)
                                                          tGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                    >  1:528736/1‑139 (MQ=255)
                                                          tGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                    <  2:528736/139‑1 (MQ=255)
                                                              ggcAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTg                                                                                      >  1:498250/1‑149 (MQ=255)
                                                                 aaTATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                             <  2:517644/91‑1 (MQ=255)
                                                                 aaTATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTcc                                                                                                                                             >  1:517644/1‑91 (MQ=255)
                                                                 aaTATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCg                                                                                   >  1:595129/1‑149 (MQ=255)
                                                                   tatTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCggg                                                                                 <  2:595129/149‑1 (MQ=255)
                                                                     ttGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGggc                                                                               >  2:583321/1‑149 (MQ=255)
                                                                     ttGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGggc                                                                               <  4:691504/149‑1 (MQ=255)
                                                                               cAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCg                                                                                                                    >  2:393388/1‑102 (MQ=255)
                                                                                aaGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTc                                                                                                   >  3:199084/1‑118 (MQ=255)
                                                                                aaGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCt                                                                    >  3:559612/1‑149 (MQ=255)
                                                                                      gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATc                                                                                                              >  3:74077/1‑101 (MQ=255)
                                                                                      gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATc                                                                                                              <  4:74077/101‑1 (MQ=255)
                                                                                      gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCttt                                                                                                    <  2:1292007/111‑1 (MQ=255)
                                                                                      gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGggc                                                                               >  3:320097/1‑132 (MQ=255)
                                                                                      gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCt                                                                    >  1:1104618/1‑143 (MQ=255)
                                                                                      gacgaTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCatg                                                              >  4:1112731/1‑149 (MQ=255)
                                                                                          aTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTAt                                                                                           >  2:902117/1‑116 (MQ=255)
                                                                                          aTACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTAt                                                                                           <  1:902117/116‑1 (MQ=255)
                                                                                           tACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCttt                                                                                                           >  1:172049/1‑99 (MQ=255)
                                                                                           tACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGtt                                                         <  2:133005/149‑1 (MQ=255)
                                                                                             cAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTgc                                                       >  4:1455526/1‑149 (MQ=255)
                                                                                               ggCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTACACGGtttg                                                                                                                                              >  3:1193122/1‑59 (MQ=255)
                                                                                                 cAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGTCATCGGCCTGGCATGATGTTGCGCtt                                                   <  2:708677/149‑1 (MQ=255)
                                                                                                 cAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCtt                                                   <  2:498250/149‑1 (MQ=255)
                                                                                                   gCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTa                                                                                                          >  2:1032298/1‑92 (MQ=255)
                                                                                                   gCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 <  2:341232/149‑1 (MQ=255)
                                                                                                          tCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACt                                          >  1:1015879/1‑149 (MQ=255)
                                                                                                           cACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGtt                                                                                                                           <  1:415524/67‑1 (MQ=255)
                                                                                                           cACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGtt                                                                                                                           >  2:415524/1‑67 (MQ=255)
                                                                                                            aCCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTTAGTTGACTaa                                        >  4:216043/1‑149 (MQ=255)
                                                                                                            aCCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 <  3:775014/140‑1 (MQ=255)
                                                                                                            aCCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 >  4:775014/1‑140 (MQ=255)
                                                                                                              cATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATTCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGTCCTGGCATGATGTTGCGCTTGAGTTGATTAAtt                                      <  1:416717/149‑1 (MQ=255)
                                                                                                                tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                  >  3:1049552/1‑87 (MQ=255)
                                                                                                                tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                  <  4:1049552/87‑1 (MQ=255)
                                                                                                                tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCa                                                                                                  >  1:259478/1‑87 (MQ=255)
                                                                                                                tCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGt                                    >  2:125034/1‑149 (MQ=255)
                                                                                                                    ggTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGtt                                                         >  3:1379554/1‑124 (MQ=255)
                                                                                                                    ggTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGtt                                                         <  4:1379554/124‑1 (MQ=255)
                                                                                                                          cGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGaa                          <  1:583321/149‑1 (MQ=255)
                                                                                                                             tAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGAGGTTGCGCTTGa                                                 >  3:976899/1‑123 (MQ=255)
                                                                                                                              aaGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAt                                                                                                         <  4:974813/66‑1 (MQ=255)
                                                                                                                              aaGGTCGTCGCCAAACCGTGTACGGTTCCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTAt                                                                                                         >  3:974813/1‑66 (MQ=255)
                                                                                                                                ggTCGTCGCCAAACCGTGTACGGTGTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAAtt                                      >  1:1241153/1‑131 (MQ=255)
                                                                                                                                  tcgtcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTgc                                                       >  2:215984/1‑112 (MQ=255)
                                                                                                                                     tcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGcc                                                                     <  4:768345/95‑1 (MQ=255)
                                                                                                                                     tcgCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAgg                  >  3:456928/1‑146 (MQ=255)
                                                                                                                                        ccAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACt            <  3:1455526/149‑1 (MQ=255)
                                                                                                                                         gaaaCCGTACACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 >  1:350047/2‑111 (MQ=255)
                                                                                                                                         gaaaCCGTACACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 <  2:350047/110‑1 (MQ=255)
                                                                                                                                          aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 <  1:176226/110‑1 (MQ=255)
                                                                                                                                          aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGa                                                 >  2:176226/1‑110 (MQ=255)
                                                                                                                                          aaaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGc          <  1:125034/149‑1 (MQ=255)
                                                                                                                                           aaCCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGcc         >  3:883975/1‑149 (MQ=255)
                                                                                                                                                  tACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTTTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTgc                                                 <  2:1240828/102‑2 (MQ=255)
                                                                                                                                                  tACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCa  >  3:200248/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACTCTTGAATATCAGTAACTGGAGATGCTCATGAAATGGTGCAAACGTGGGTATGTATTGGCGGCAATATTGGCGCTCGCAAGTGCGACGATACAGGCAGCCGATGTCACCATCACGGTGAACGGTAAGGTCGTCGCCAAACCGTGTACGGTTTCCACCACCAATGCCACGGTTGATCTCGGCGATCTTTATTCTTTCAGTCTTATGTCTGCCGGGGCGGCATCGGCCTGGCATGATGTTGCGCTTGAGTTGACTAATTGTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCA  >  NC_000913/4548255‑4548549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: