Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,632,723 G→A 5.2% intergenic (+53/‑37) slt → / → trpR lytic murein transglycosylase, soluble/transcriptional repressor, tryptophan‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,632,7230GA 5.2% 449.6 / 6.7 132intergenic (+53/‑37)slt/trpRlytic murein transglycosylase, soluble/transcriptional repressor, tryptophan‑binding
Reads supporting (aligned to +/- strand):  ref base G (68/57);  new base A (4/3);  total (72/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 134 total aligned reads displayed.

GAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCG  >  NC_000913/4632577‑4632870
                                                                                                                                                  |                                                                                                                                                   
gaagaaCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGc                                                                                                                                                   >  4:1285316/1‑149 (MQ=255)
 aagaaCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAAGCa                                                                                                                                                  >  2:543008/1‑149 (MQ=255)
     aCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtttt                                                                                                                                              <  2:837843/149‑1 (MQ=255)
          cTGGCTTATGACGCTTACTACCGCTATTTCATGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTcc                                                                                                                                        <  1:1031521/149‑1 (MQ=255)
               ttATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtttt                                                                                                                                              >  1:557590/1‑139 (MQ=255)
                     cGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAAGCAAAATGCCTccccc                                                                                                                                     >  1:537090/1‑142 (MQ=255)
                              ccGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTc                                                                                                                                         <  3:1014275/129‑1 (MQ=255)
                               cGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAAc                                                                                                                               >  4:410374/1‑138 (MQ=255)
                               cGCTATTGCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAAc                                                                                                                               <  3:410374/138‑1 (MQ=255)
                                 cTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTaa                                                                                                                                <  2:642832/135‑1 (MQ=255)
                                 cTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTaa                                                                                                                                >  1:642832/1‑135 (MQ=255)
                                 cTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACatat                                                                                                                  <  3:1285316/149‑1 (MQ=255)
                                     ttCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATg                                                                                                              <  3:58904/149‑1 (MQ=255)
                                           ggggATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACAtatt                                                                                                                 >  2:1058902/1‑140 (MQ=255)
                                           ggggATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCcaa                                                                                                        >  1:627547/1‑149 (MQ=255)
                                           ggggATAAACCGACGTTGATGAGCGCCACGGAATAGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACAtatt                                                                                                                 <  1:1058902/140‑1 (MQ=255)
                                              gATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtttt                                                                                                                                              >  1:33854/1‑108 (MQ=255)
                                              gATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtttt                                                                                                                                              <  2:33854/108‑1 (MQ=255)
                                              gATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCcaacaa                                                                                                     >  3:554829/1‑149 (MQ=255)
                                               aTAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAAt                                                                                                    >  3:241887/1‑149 (MQ=255)
                                                 aaaCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGAAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCa                                                                                                  >  1:1374910/1‑149 (MQ=255)
                                                  aaCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCAc                                                                                                 <  2:1354592/149‑1 (MQ=255)
                                                   aCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCtt                                                                                                                                          <  2:512358/107‑1 (MQ=255)
                                                   aCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCtt                                                                                                                                          >  1:512358/1‑107 (MQ=255)
                                                   aCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACAtatta                                                                                                                <  4:167566/133‑1 (MQ=255)
                                                   aCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCAcc                                                                                                <  2:627547/149‑1 (MQ=255)
                                                     cGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCt                                                                                              >  2:544559/1‑149 (MQ=255)
                                                         gTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAttt                                                                                                                                               >  2:1456733/1‑96 (MQ=255)
                                                          ttGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCcaacaa                                                                                                     >  3:137538/1‑137 (MQ=255)
                                                          ttGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCcaacaa                                                                                                     <  4:137538/137‑1 (MQ=255)
                                                           tgatgaGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtt                                                                                                                                                <  3:1183567/93‑1 (MQ=255)
                                                           tgatgaGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCAtt                                                                                                                                                >  4:1183567/1‑93 (MQ=255)
                                                             atgaGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTcagca                                                                                      <  1:544559/149‑1 (MQ=255)
                                                                  cgcCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGAt                                                                                 <  4:554829/149‑1 (MQ=255)
                                                                      aCGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTcag                                                                                        <  2:27272/138‑1 (MQ=255)
                                                                      aCGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTcag                                                                                        >  1:27272/1‑138 (MQ=255)
                                                                      aCGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCa                                                                             >  1:1447451/1‑149 (MQ=255)
                                                                      aCGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCa                                                                             >  2:459543/1‑149 (MQ=255)
                                                                         gAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAAGCaaaat                                                                                                                                              <  2:1012797/81‑5 (MQ=255)
                                                                         gAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAAGCaaaat                                                                                                                                              >  1:1012797/1‑77 (MQ=255)
                                                                          aaTGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTcagca                                                                                      >  4:1067094/1‑136 (MQ=255)
                                                                          aaTGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTcagca                                                                                      <  3:1067094/136‑1 (MQ=255)
                                                                             ggggACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCAcc                                                                                                >  1:1294240/1‑123 (MQ=255)
                                                                             ggggACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCa                                                                             <  3:183671/142‑1 (MQ=255)
                                                                               ggACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAAGCAAAATGCCTCCCCCggtt                                                                                                                                 >  3:800154/1‑85 (MQ=37)
                                                                                 aCGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCa                                                                  >  3:199493/1‑149 (MQ=255)
                                                                                    tcgtTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAAt                                                                                                    <  3:790950/112‑1 (MQ=255)
                                                                                    tcgtTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCa                                                               >  2:685157/1‑149 (MQ=255)
                                                                                    tcgtTACTGATCCGCACGTTTATGATATGCTATCCTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCa                                                               >  2:1392057/1‑149 (MQ=255)
                                                                                      gtTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAgg                                                             >  3:278064/1‑149 (MQ=255)
                                                                                       ttACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACAtattat                                                                                                               >  1:802550/1‑98 (MQ=255)
                                                                                       ttACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGa                                                            <  2:591643/149‑1 (MQ=255)
                                                                                         aCTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGc                                                                      <  2:295310/137‑1 (MQ=255)
                                                                                         aCTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGt                                                          <  1:685157/149‑1 (MQ=255)
                                                                                         aCTGATCCGCACGTTTATGATATGCTATCCTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGt                                                          <  1:1392057/149‑1 (MQ=255)
                                                                                           tGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTgg                                                        >  1:702933/1‑149 (MQ=255)
                                                                                           tGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTgg                                                        >  2:64548/1‑149 (MQ=255)
                                                                                            gATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGt                                                       <  4:199493/149‑1 (MQ=255)
                                                                                                 gCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGt                                                  <  1:1086445/149‑1 (MQ=255)
                                                                                                 gCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTGTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGt                                                  >  1:956846/1‑149 (MQ=255)
                                                                                                   aCGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAg                                                                       <  1:68215/126‑1 (MQ=255)
                                                                                                      tttATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGt                                             <  4:348191/149‑1 (MQ=255)
                                                                                                         aTGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTg                                                         <  1:110725/134‑1 (MQ=255)
                                                                                                         aTGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTg                                                         >  2:110725/1‑134 (MQ=255)
                                                                                                           gATATGCTATCGTACTCTTTGGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAcc                                        >  2:58454/1‑149 (MQ=255)
                                                                                                           gATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAcc                                        >  3:151469/1‑149 (MQ=255)
                                                                                                              atGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGc                                     <  4:278064/149‑1 (MQ=255)
                                                                                                              atGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGc                                     <  4:643638/149‑1 (MQ=255)
                                                                                                                  tATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTg                                              <  1:509114/136‑1 (MQ=255)
                                                                                                                  tATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTaa                                 >  2:1212225/1‑149 (MQ=255)
                                                                                                                  tATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTaa                                 >  2:177527/1‑149 (MQ=255)
                                                                                                                      gTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACa                                                                                                                     <  2:459965/61‑1 (MQ=255)
                                                                                                                      gTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAAt                             >  1:918563/1‑149 (MQ=255)
                                                                                                                      gTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAAt                             <  2:702933/149‑1 (MQ=255)
                                                                                                                       tACTCTTTAGCGAGTACAACCAGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACATCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATg                            >  4:10885/1‑149 (MQ=255)
                                                                                                                        aCTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGc                           <  1:177527/149‑1 (MQ=255)
                                                                                                                        aCTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGc                           >  2:1271337/1‑149 (MQ=255)
                                                                                                                          tctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATgg                                                                                                                          >  2:1064666/1‑52 (MQ=255)
                                                                                                                           ctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTcagca                                                                                      >  1:399282/1‑87 (MQ=255)
                                                                                                                            tttAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGt                                                       >  1:635231/1‑117 (MQ=255)
                                                                                                                            tttAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTc                                            <  2:191279/128‑1 (MQ=255)
                                                                                                                            tttAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTc                                            >  1:191279/1‑128 (MQ=255)
                                                                                                                            tttAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAc                       >  1:1213069/1‑149 (MQ=255)
                                                                                                                            tttAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAc                       <  4:1068199/149‑1 (MQ=255)
                                                                                                                                gCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCaaa                   >  3:154695/1‑149 (MQ=255)
                                                                                                                                gCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCaaa                   >  1:582126/1‑149 (MQ=255)
                                                                                                                                 cGAGTACAACCGGGGGAAGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCaaaa                  <  4:154695/149‑1 (MQ=255)
                                                                                                                                  gAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAAc                 <  1:459543/149‑1 (MQ=255)
                                                                                                                                   aGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACg                <  1:1102116/149‑1 (MQ=255)
                                                                                                                                    gTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGa               <  1:1271337/149‑1 (MQ=255)
                                                                                                                                     tACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGc                                                                      <  3:1252971/93‑1 (MQ=255)
                                                                                                                                     tACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGc                                                                      >  4:1252971/1‑93 (MQ=255)
                                                                                                                                     tACAACCGGGGAAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAt              <  2:1374910/149‑1 (MQ=255)
                                                                                                                                        aaCCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGcc                          >  2:722176/1‑134 (MQ=255)
                                                                                                                                        aaCCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAtctc           >  3:178600/1‑149 (MQ=255)
                                                                                                                                        aaCCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAtctc           >  2:673036/1‑149 (MQ=255)
                                                                                                                                         aCCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGGCATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTa                        >  4:241187/1‑135 (MQ=255)
                                                                                                                                                gAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTAc    <  3:284259/148‑1 (MQ=255)
                                                                                                                                                  ggCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTCACGTTTTGTCGACCTGCTTaa                                 >  3:657164/1‑117 (MQ=255)
                                                                                                                                                  ggCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTCACGTTTTGTCGACCTGCTTaa                                 <  4:657164/117‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCG  >  NC_000913/4632577‑4632870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: