Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,150 A→C 5.4% intergenic (+64/+83) trpR → / ← yjjX transcriptional repressor, tryptophan‑binding/non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,1500AC 5.4% 262.7 / 5.9 74intergenic (+64/+83)trpR/yjjXtranscriptional repressor, tryptophan‑binding/non‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base C (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCG  >  NC_000913/4633003‑4633298
                                                                                                                                                   |                                                                                                                                                    
aTTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGaa                                                                                                                                                     <  1:881472/149‑1 (MQ=255)
aTTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGaa                                                                                                                                                     <  2:1396282/149‑1 (MQ=255)
    cgcgTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGcc                                                                                                                                                 <  3:890447/149‑1 (MQ=255)
       gTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                              >  4:276833/1‑149 (MQ=255)
           aTCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                          >  4:1468748/1‑149 (MQ=255)
              tAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       >  4:699294/1‑149 (MQ=255)
               aaCAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                                                                                                      >  1:1250607/1‑149 (MQ=255)
                     cTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       >  1:191323/1‑142 (MQ=255)
                     cTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       <  2:191323/142‑1 (MQ=255)
                      tGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGc                                                                                                                                                  >  4:704558/1‑130 (MQ=255)
                      tGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGc                                                                                                                                                  <  3:704558/130‑1 (MQ=255)
                       gAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAc                                                                                                                              >  4:395043/1‑149 (MQ=255)
                       gAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAc                                                                                                                              <  2:1250607/149‑1 (MQ=255)
                        aaaGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       >  2:1226655/1‑139 (MQ=255)
                        aaaGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       <  1:1226655/139‑1 (MQ=255)
                         aaGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAccc                                                                                                                            >  1:724948/1‑149 (MQ=255)
                             cgcgCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTaa                                                                                                                        <  3:276833/149‑1 (MQ=255)
                              gcgcCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGTAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       >  4:512576/1‑133 (MQ=255)
                              gcgcCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       <  3:512576/133‑1 (MQ=255)
                                   cGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCaa                                                                                                                  <  3:1468748/149‑1 (MQ=255)
                                         gCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAACGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                             >  1:1414143/1‑116 (MQ=255)
                                         gCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                             <  2:1414143/116‑1 (MQ=255)
                                            gcgcCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTgg                                                                                                         <  2:16518/149‑1 (MQ=255)
                                            gcgcCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTCCAATATGTTTgg                                                                                                         <  2:724948/149‑1 (MQ=255)
                                               ccAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAg                                                                                                      <  3:1277982/149‑1 (MQ=255)
                                               ccAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAg                                                                                                      <  3:699294/149‑1 (MQ=255)
                                                 aGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGc                                                                                                    <  3:395043/149‑1 (MQ=255)
                                                  gTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAc                                                                                                                              >  4:111458/1‑122 (MQ=255)
                                                  gTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAc                                                                                                                              <  3:111458/122‑1 (MQ=255)
                                                     gCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       >  1:211506/1‑110 (MQ=255)
                                                     gCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                       <  2:211506/110‑1 (MQ=255)
                                                       tGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAc                                                                                                                              <  2:769841/117‑1 (MQ=255)
                                                       tGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGc                                                                                                    >  3:1157053/1‑143 (MQ=255)
                                                       tGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGc                                                                                                    <  4:1157053/143‑1 (MQ=255)
                                                       tGGAAGAGGTGATGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATAc                                                                                                                              >  1:769841/1‑117 (MQ=255)
                                                            gagGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                                                                                        >  1:429615/1‑102 (MQ=255)
                                                            gagGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                                                                                        <  2:429615/102‑1 (MQ=255)
                                                               gtgtTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCgg                                                                                      >  3:198868/1‑149 (MQ=255)
                                                                       gAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                                                                                        >  2:97083/1‑91 (MQ=255)
                                                                       gAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                                                                                        <  1:97083/91‑1 (MQ=255)
                                                                         aaaGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATGCGACGCTGCCGCGTctta                                                                            <  1:955196/149‑4 (MQ=37)
                                                                                ttgattTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGgcg                                                                     >  4:1422453/1‑149 (MQ=255)
                                                                                 tgattTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGgcgc                                                                    <  4:198868/149‑1 (MQ=255)
                                                                                            gCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                   >  4:573527/1‑139 (MQ=255)
                                                                                            gCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                   <  3:573527/139‑1 (MQ=255)
                                                                                                 aTAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGt                                                                                                             <  4:513455/92‑1 (MQ=255)
                                                                                                 aTAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGt                                                                                                             >  3:513455/1‑92 (MQ=255)
                                                                                                          ggCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGa                                           <  3:1422453/149‑1 (MQ=255)
                                                                                                           gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAgg                                                                       >  2:1392616/1‑120 (MQ=255)
                                                                                                           gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAgg                                                                       <  1:1392616/120‑1 (MQ=255)
                                                                                                             gcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGttt                                                                                                           <  3:1421256/82‑1 (MQ=255)
                                                                                                             gcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGttt                                                                                                           >  4:1421256/1‑82 (MQ=255)
                                                                                                             gcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATaa                                                                                             >  3:967689/1‑96 (MQ=255)
                                                                                                             gcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATaa                                                                                             <  4:967689/96‑1 (MQ=255)
                                                                                                             gcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTc                                        >  1:1101992/1‑149 (MQ=255)
                                                                                                               aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGc                                                                             >  4:961946/1‑110 (MQ=255)
                                                                                                               aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGAGGCAGCGTCGc                                                                             <  3:961946/110‑1 (MQ=255)
                                                                                                                      aTCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATa                                                              <  1:1389871/118‑1 (MQ=255)
                                                                                                                      aTCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATa                                                              >  2:1389871/1‑118 (MQ=255)
                                                                                                                            cACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa                                               <  3:272044/127‑1 (MQ=255)
                                                                                                                            cACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa                                               >  2:380354/1‑127 (MQ=255)
                                                                                                                            cACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa                                               >  4:272044/1‑127 (MQ=255)
                                                                                                                            cACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa                                               <  1:380354/127‑1 (MQ=255)
                                                                                                                             aCCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCg                        >  2:323712/1‑149 (MQ=255)
                                                                                                                               cGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCa                                                                                                   >  4:974736/1‑72 (MQ=255)
                                                                                                                               cGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCa                                                                                                   <  3:974736/72‑1 (MQ=255)
                                                                                                                                gAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                     <  1:323712/149‑1 (MQ=255)
                                                                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGt                  <  2:1101992/149‑1 (MQ=255)
                                                                                                                                    gacGGATAAGGCGTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGc                                                                             >  4:339916/3‑89 (MQ=37)
                                                                                                                                    gacGGATAAGGCGTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGc                                                                             <  3:339916/87‑1 (MQ=25)
                                                                                                                                     ccGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGa                >  2:10379/1‑149 (MQ=255)
                                                                                                                                     ccGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGa                >  1:541129/1‑149 (MQ=255)
                                                                                                                                         ataaggcgTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGgcgc                                                                    <  2:1303556/89‑1 (MQ=37)
                                                                                                                                         ataaggcgTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGgcgc                                                                    >  1:1303556/5‑93 (MQ=37)
                                                                                                                                             ggcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGt                                                                                <  3:1017353/77‑1 (MQ=255)
                                                                                                                                             ggcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGt                                                                                >  4:1017353/1‑77 (MQ=255)
                                                                                                                                                   aaCGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGcgcg  <  1:10379/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCG  >  NC_000913/4633003‑4633298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: