Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 446,789 A→G 12.4% intergenic (‑123/+26) yajR ← / ← cyoE putative transporter/protoheme IX farnesyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913446,7890AG12.4% 133.4 / 7.9 48intergenic (‑123/+26)yajR/cyoEputative transporter/protoheme IX farnesyltransferase
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base G (3/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCT  >  NC_000913/446649‑446934
                                                                                                                                            |                                                                                                                                                 
cATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttt                                                                                                                                           <  4:18990/149‑1 (MQ=255)
                        ctctGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACca                                                                                                                    >  2:881475/1‑148 (MQ=255)
                        ctctGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACca                                                                                                                    <  1:881475/148‑1 (MQ=255)
                         tctGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttt                                                                                                                                           >  3:492236/1‑124 (MQ=255)
                         tctGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttt                                                                                                                                           <  4:492236/124‑1 (MQ=255)
                                 aaaTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttt                                                                                                                                           >  4:615743/1‑116 (MQ=255)
                                 aaaTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttt                                                                                                                                           <  3:615743/116‑1 (MQ=255)
                                              gtgtAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTATTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttt                                                                                                                                           <  1:728983/103‑1 (MQ=255)
                                              gtgtAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttt                                                                                                                                           >  2:728983/1‑103 (MQ=255)
                                                 tAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCtttt                                                                                                                                          <  4:707512/101‑1 (MQ=255)
                                                 tAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCtttt                                                                                                                                          >  3:707512/1‑101 (MQ=255)
                                                        gCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttt                                                                                                                                           <  1:1124140/93‑1 (MQ=255)
                                                        gCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttt                                                                                                                                           >  2:1124140/1‑93 (MQ=255)
                                                               aaTGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTa                                                                                                 <  1:700951/128‑1 (MQ=255)
                                                               aaTGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTa                                                                                                 >  2:700951/1‑128 (MQ=255)
                                                                     gTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                       >  3:368553/1‑148 (MQ=255)
                                                                     gTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                       <  4:368553/148‑1 (MQ=255)
                                                                              cGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATagaga                                                                                                                                 >  2:678133/1‑81 (MQ=255)
                                                                              cGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATagaga                                                                                                                                 <  1:678133/81‑1 (MQ=255)
                                                                                gATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCAc                                                           >  3:1194/1‑149 (MQ=255)
                                                                                   tttttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCga                                                        >  3:1404467/1‑149 (MQ=255)
                                                                                      ttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGagag                                                     <  4:1194/149‑1 (MQ=255)
                                                                                         gTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                 >  3:1416466/1‑118 (MQ=255)
                                                                                         gTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                 <  4:1416466/118‑1 (MQ=255)
                                                                                          tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCa                                                 <  4:1404467/149‑1 (MQ=255)
                                                                                                      gagGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc                                                                                              <  1:1039391/92‑1 (MQ=255)
                                                                                                      gagGGAGATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc                                                                                              >  2:1039391/1‑92 (MQ=255)
                                                                                                         ggATATGTTTCTAATGCTAAGGAATAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  >  3:819474/1‑149 (MQ=255)
                                                                                                          gATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  <  1:696069/148‑1 (MQ=255)
                                                                                                          gATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  >  2:696069/1‑148 (MQ=255)
                                                                                                                tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGc                           >  4:730449/1‑149 (MQ=255)
                                                                                                                          tAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACTCAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCtt                 >  2:1251347/1‑149 (MQ=255)
                                                                                                                          tAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAggg                                                   <  2:740717/115‑1 (MQ=255)
                                                                                                                          tAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAggg                                                   >  1:740717/1‑115 (MQ=255)
                                                                                                                          tAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCtt                 <  1:1251347/149‑1 (MQ=255)
                                                                                                                           aaGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGt                                               <  2:329475/118‑1 (MQ=255)
                                                                                                                           aaGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGt                                               >  1:329475/1‑118 (MQ=255)
                                                                                                                            aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATgg                                          >  3:1394906/1‑122 (MQ=255)
                                                                                                                            aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATgg                                          <  4:1394906/122‑1 (MQ=255)
                                                                                                                              aaaaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                       <  2:451951/91‑1 (MQ=255)
                                                                                                                              aaaaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                       >  1:451951/1‑91 (MQ=255)
                                                                                                                                    ggTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGccc         <  2:501464/147‑1 (MQ=255)
                                                                                                                                    ggTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGccc         >  1:501464/1‑147 (MQ=255)
                                                                                                                                       gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGCCATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGAtt    <  4:819474/149‑1 (MQ=255)
                                                                                                                                        ccGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTc   >  2:901566/1‑149 (MQ=255)
                                                                                                                                         cGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCt  <  1:901566/149‑1 (MQ=255)
                                                                                                                                           tAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  >  2:882981/1‑115 (MQ=255)
                                                                                                                                           tAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  <  1:882981/115‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
CATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCT  >  NC_000913/446649‑446934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑