Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 489,963 G→C 9.1% intergenic (+65/+147) mscK → / ← ybaM mechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,9630GC 9.1% 224.3 / 12.1 66intergenic (+65/+147)mscK/ybaMmechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein
Reads supporting (aligned to +/- strand):  ref base G (29/31);  new base C (3/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

TCTGCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGC  >  NC_000913/489817‑490070
                                                                                                                                                  |                                                                                                           
tCTGCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGAt                                                                                                           >  4:3462/1‑149 (MQ=255)
  tacacaACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGAGCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgc                                                                                                         <  4:1256992/147‑1 (MQ=255)
        aCGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTg                                                                                                   >  4:1308611/1‑149 (MQ=255)
                   gATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        >  4:537572/1‑149 (MQ=255)
                        ggTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGcc                                                                                   <  3:537572/149‑1 (MQ=255)
                         gTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        >  2:543677/1‑143 (MQ=255)
                         gTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        <  1:543677/143‑1 (MQ=255)
                           gACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc                                                                                <  4:431123/149‑1 (MQ=255)
                           gACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc                                                                                <  3:1308611/149‑1 (MQ=255)
                             cGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGa                                                                              <  3:3462/149‑1 (MQ=255)
                                     aaaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGc                                                                                              <  3:1197767/125‑1 (MQ=255)
                                     aaaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGc                                                                                              >  4:1197767/1‑125 (MQ=255)
                                      aaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGcc                                                                                             <  3:141234/125‑1 (MQ=255)
                                      aaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGcc                                                                                             >  4:141234/1‑125 (MQ=255)
                                       aCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAg                                                                     >  4:1000943/1‑148 (MQ=255)
                                       aCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAg                                                                     <  3:1000943/148‑1 (MQ=255)
                                           gACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                 >  1:1257205/1‑115 (MQ=255)
                                           gACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                 <  2:1257205/116‑2 (MQ=255)
                                                  aaGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAatatat                                                         >  1:1395451/1‑149 (MQ=255)
                                                       gATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        <  1:487684/113‑1 (MQ=255)
                                                       gATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        >  2:487684/1‑113 (MQ=255)
                                                             ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgcg                                                                                                        <  3:992683/91‑1 (MQ=255)
                                                             ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgcg                                                                                                        >  4:992683/1‑91 (MQ=255)
                                                             ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                 >  1:538787/1‑97 (MQ=255)
                                                             ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                 <  2:538787/98‑2 (MQ=255)
                                                             ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                 >  1:57143/1‑97 (MQ=255)
                                                             ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa                                                                                                 <  2:57143/98‑2 (MQ=255)
                                                                 cGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTa                                          <  2:1395451/149‑1 (MQ=255)
                                                                         gTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc                                                                                >  2:759652/1‑103 (MQ=255)
                                                                         gTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc                                                                                <  1:759652/103‑1 (MQ=255)
                                                                               tAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        <  4:1389143/89‑1 (MQ=255)
                                                                               tAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc                                                                                        >  3:1389143/1‑89 (MQ=255)
                                                                                aaaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc                              <  2:259911/146‑1 (MQ=255)
                                                                                aaaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc                              >  1:259911/1‑146 (MQ=255)
                                                                                 aaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGa                                                                                                  <  3:661071/77‑1 (MQ=255)
                                                                                 aaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGa                                                                                                  >  4:661071/1‑77 (MQ=255)
                                                                                  aaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAgc                         >  3:1346889/1‑149 (MQ=255)
                                                                                    aCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAgcgc                       <  4:1346889/149‑1 (MQ=255)
                                                                                      gAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCg                                                                                      >  4:586486/1‑84 (MQ=255)
                                                                                      gAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCg                                                                                      <  3:586486/84‑1 (MQ=255)
                                                                                              gCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgtgt                                           <  2:765831/119‑1 (MQ=255)
                                                                                              gCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgtgt                                           >  1:765831/1‑119 (MQ=255)
                                                                                                aaCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                              >  1:1049/1‑98 (MQ=255)
                                                                                                aaCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                              <  2:1049/98‑1 (MQ=255)
                                                                                                 aCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCt                                                                                            <  2:1335425/67‑1 (MQ=255)
                                                                                                 aCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCt                                                                                            >  1:1335425/1‑67 (MQ=255)
                                                                                                    tttAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgt                                             <  2:31458/111‑1 (MQ=255)
                                                                                                    tttAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgt                                             >  1:31458/1‑111 (MQ=255)
                                                                                                             cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCt                                     <  3:170675/110‑1 (MQ=255)
                                                                                                             cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCt                                     >  4:170675/1‑110 (MQ=255)
                                                                                                             cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc  <  3:1204017/145‑1 (MQ=255)
                                                                                                             cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc  >  4:1204017/1‑145 (MQ=255)
                                                                                                              ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGAt                                  >  2:1315828/1‑112 (MQ=255)
                                                                                                              ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGAt                                  <  1:1315828/112‑1 (MQ=255)
                                                                                                              ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc  >  2:1238370/1‑144 (MQ=255)
                                                                                                              ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc  <  1:1238370/144‑1 (MQ=255)
                                                                                                                     tGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                              <  4:1137464/77‑1 (MQ=255)
                                                                                                                     tGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                              >  3:1137464/1‑77 (MQ=255)
                                                                                                                         aaCAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCa                                                     >  3:217249/1‑82 (MQ=255)
                                                                                                                         aaCAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCa                                                     <  4:217249/82‑1 (MQ=255)
                                                                                                                                      ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGc                                       <  3:643541/83‑1 (MQ=255)
                                                                                                                                      ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGc                                       >  4:643541/1‑83 (MQ=255)
                                                                                                                                      ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc                              >  4:504905/1‑92 (MQ=255)
                                                                                                                                      ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc                              <  3:504905/92‑1 (MQ=255)
                                                                                                                                              gCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                              <  2:1040060/52‑1 (MQ=255)
                                                                                                                                              gCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa                                                              >  1:1040060/1‑52 (MQ=255)
                                                                                                                                                  |                                                                                                           
TCTGCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGC  >  NC_000913/489817‑490070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: