| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 489,963 | G→C | 9.1% | intergenic (+65/+147) | mscK → / ← ybaM | mechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 489,963 | 0 | G | C | 9.1% | 224.3 / 12.1 | 66 | intergenic (+65/+147) | mscK/ybaM | mechanosensitive channel protein, intermediate conductance, K+ regulated/DUF2496 family protein |
| Reads supporting (aligned to +/- strand): ref base G (29/31); new base C (3/3); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
TCTGCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGC > NC_000913/489817‑490070 | tCTGCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGAt > 4:3462/1‑149 (MQ=255) tacacaACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGAGCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgc < 4:1256992/147‑1 (MQ=255) aCGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTg > 4:1308611/1‑149 (MQ=255) gATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc > 4:537572/1‑149 (MQ=255) ggTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGcc < 3:537572/149‑1 (MQ=255) gTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc > 2:543677/1‑143 (MQ=255) gTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc < 1:543677/143‑1 (MQ=255) gACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc < 4:431123/149‑1 (MQ=255) gACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc < 3:1308611/149‑1 (MQ=255) cGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGa < 3:3462/149‑1 (MQ=255) aaaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGc < 3:1197767/125‑1 (MQ=255) aaaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGc > 4:1197767/1‑125 (MQ=255) aaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGcc < 3:141234/125‑1 (MQ=255) aaCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGcc > 4:141234/1‑125 (MQ=255) aCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAg > 4:1000943/1‑148 (MQ=255) aCGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAg < 3:1000943/148‑1 (MQ=255) gACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa > 1:1257205/1‑115 (MQ=255) gACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa < 2:1257205/116‑2 (MQ=255) aaGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAatatat > 1:1395451/1‑149 (MQ=255) gATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc < 1:487684/113‑1 (MQ=255) gATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc > 2:487684/1‑113 (MQ=255) ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgcg < 3:992683/91‑1 (MQ=255) ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATgcg > 4:992683/1‑91 (MQ=255) ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa > 1:538787/1‑97 (MQ=255) ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa < 2:538787/98‑2 (MQ=255) ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa > 1:57143/1‑97 (MQ=255) ccGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCACGCCGCATCCGGCATGaa < 2:57143/98‑2 (MQ=255) cGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTa < 2:1395451/149‑1 (MQ=255) gTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc > 2:759652/1‑103 (MQ=255) gTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTAc < 1:759652/103‑1 (MQ=255) tAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc < 4:1389143/89‑1 (MQ=255) tAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATc > 3:1389143/1‑89 (MQ=255) aaaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc < 2:259911/146‑1 (MQ=255) aaaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc > 1:259911/1‑146 (MQ=255) aaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGa < 3:661071/77‑1 (MQ=255) aaaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGa > 4:661071/1‑77 (MQ=255) aaaCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAgc > 3:1346889/1‑149 (MQ=255) aCGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAgcgc < 4:1346889/149‑1 (MQ=255) gAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCg > 4:586486/1‑84 (MQ=255) gAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCg < 3:586486/84‑1 (MQ=255) gCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgtgt < 2:765831/119‑1 (MQ=255) gCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgtgt > 1:765831/1‑119 (MQ=255) aaCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa > 1:1049/1‑98 (MQ=255) aaCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa < 2:1049/98‑1 (MQ=255) aCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCt < 2:1335425/67‑1 (MQ=255) aCATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCt > 1:1335425/1‑67 (MQ=255) tttAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgt < 2:31458/111‑1 (MQ=255) tttAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGgt > 1:31458/1‑111 (MQ=255) cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCt < 3:170675/110‑1 (MQ=255) cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCt > 4:170675/1‑110 (MQ=255) cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc < 3:1204017/145‑1 (MQ=255) cccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc > 4:1204017/1‑145 (MQ=255) ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGAt > 2:1315828/1‑112 (MQ=255) ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGAt < 1:1315828/112‑1 (MQ=255) ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc > 2:1238370/1‑144 (MQ=255) ccGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGc < 1:1238370/144‑1 (MQ=255) tGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa < 4:1137464/77‑1 (MQ=255) tGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa > 3:1137464/1‑77 (MQ=255) aaCAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCa > 3:217249/1‑82 (MQ=255) aaCAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCa < 4:217249/82‑1 (MQ=255) ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGc < 3:643541/83‑1 (MQ=255) ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGc > 4:643541/1‑83 (MQ=255) ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc > 4:504905/1‑92 (MQ=255) ttgttCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGc < 3:504905/92‑1 (MQ=255) gCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa < 2:1040060/52‑1 (MQ=255) gCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCaa > 1:1040060/1‑52 (MQ=255) | TCTGCACAACGAGAAGGGCGATGAGGTGACGGAAGTAAAACGCGACTACAAAGGCGATGACCCGACGCCAGCGGTAGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGCGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGC > NC_000913/489817‑490070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |