Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 512,212 T→G 5.3% V191G (GTG→GGG)  glsA → glutaminase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913512,2120TG 5.3% 551.2 / 18.0 151V191G (GTG→GGG) glsAglutaminase 1
Reads supporting (aligned to +/- strand):  ref base T (70/73);  new base G (5/3);  total (75/76)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 164 total aligned reads displayed.

ATCCAACAGCAACTGGCTGGCGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAATGATGATG  >  NC_000913/512064‑512360
                                                                                                                                                    |                                                                                                                                                    
   cAACAGCAACTGGCTGGCGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGta                                                                                                                                                   <  4:208466/149‑1 (MQ=255)
        gCAACTGGCTGGCGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATAccc                                                                                                                                              <  2:903512/149‑1 (MQ=255)
        gCAACTGGCTGGCGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATAccc                                                                                                                                              <  1:139275/149‑1 (MQ=255)
                 tggcGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCt                                                                                                                                     >  3:1231725/1‑149 (MQ=255)
                  ggcGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTc                                                                                                                                    <  1:75209/149‑1 (MQ=255)
                           gTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGctcct                                                                                                                           >  3:762776/1‑149 (MQ=255)
                                       gACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACgt                                                                                                                                                      >  3:1430774/1‑110 (MQ=255)
                                       gACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACgt                                                                                                                                                      <  4:1430774/110‑1 (MQ=255)
                                        aCGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTGCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGATgt                                                                                                                                                      >  4:1270466/1‑109 (MQ=255)
                                          gAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACt                                                                                                            <  1:556452/149‑1 (MQ=255)
                                             gTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCaa                                                                                                                        <  2:660472/134‑1 (MQ=255)
                                             gTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGc                                                                                                         >  3:392876/1‑149 (MQ=255)
                                                aaCCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGctcctc                                                                                                                          >  1:1006994/1‑129 (MQ=255)
                                                aaCCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAAc                                                                                                      >  4:1336933/1‑149 (MQ=255)
                                                 aCCAGTCGCAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACg                                                                                                     >  4:123430/1‑149 (MQ=255)
                                                  ccAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGc                                                                                                    <  3:360526/149‑1 (MQ=255)
                                                    aGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCtt                                                                                                  >  4:16991/1‑149 (MQ=255)
                                                     gTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATTccc                                                                                                                                              >  3:1209695/1‑104 (MQ=255)
                                                      tCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTgg                                                                                                >  2:958160/1‑149 (MQ=255)
                                                         gAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGgcg                                                                                              >  2:488940/1‑148 (MQ=255)
                                                         gAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGgcg                                                                                              <  1:488940/148‑1 (MQ=255)
                                                           acaaacaaCCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGa                                                                                           >  2:266432/1‑149 (MQ=255)
                                                              aacaacCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGc                                                                                        >  1:247654/1‑149 (MQ=255)
                                                                 aaccaacTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACgt                                                                                                                                                      <  4:894776/84‑1 (MQ=255)
                                                                 aaccaacTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGaa                                                                                                              <  1:1006671/124‑1 (MQ=255)
                                                                 aaccaacTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGaa                                                                                                              >  2:1006671/1‑124 (MQ=255)
                                                                 aaccaacTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTgg                                                                                     >  3:14038/1‑149 (MQ=255)
                                                                     aacTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTggcggc                                                                                 >  4:1442481/1‑149 (MQ=255)
                                                                      acTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTAtt                                                                                                                 >  1:1395648/1‑116 (MQ=255)
                                                                      acTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTggcggc                                                                                 <  4:1072487/148‑1 (MQ=255)
                                                                      acTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCa                                                                                >  1:431546/1‑149 (MQ=255)
                                                                        ttCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACgt                                                                                                                                                      >  1:1049912/1‑77 (MQ=255)
                                                                        ttCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACgt                                                                                                                                                      <  2:1049912/77‑1 (MQ=255)
                                                                          ccATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAggtg                                                                            <  3:1336933/149‑1 (MQ=255)
                                                                            aTAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTgg                                                                                                          <  3:234096/117‑1 (MQ=255)
                                                                            aTAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGc                                                                                                         <  4:508315/118‑1 (MQ=255)
                                                                             tAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGgtggtg                                                                         <  3:1442481/149‑1 (MQ=255)
                                                                              aaCCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGgtgt                                                                        <  3:940627/149‑1 (MQ=255)
                                                                              aaCCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGgtgt                                                                        <  1:1060634/149‑1 (MQ=255)
                                                                              aaCCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGgtgt                                                                        <  4:762776/149‑1 (MQ=255)
                                                                               aCCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTGAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGtgtg                                                                       >  2:1163861/1‑149 (MQ=255)
                                                                                 cGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTg                                                                                                                                       >  1:195136/1‑83 (MQ=255)
                                                                                 cGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTg                                                                                                                                       <  2:195136/83‑1 (MQ=255)
                                                                                    gCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATcc                                                                  <  4:14038/149‑1 (MQ=255)
                                                                                    gCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATcc                                                                  <  4:59652/149‑1 (MQ=255)
                                                                                    gCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATcc                                                                  <  2:247654/149‑1 (MQ=255)
                                                                                     ccATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCg                                                                 <  3:1026893/149‑1 (MQ=255)
                                                                                       aTAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGtt                                                               <  4:392876/149‑1 (MQ=255)
                                                                                         aGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGCACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGa                                                             <  2:431546/149‑1 (MQ=255)
                                                                                         aGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGt                                                                <  4:94840/146‑1 (MQ=255)
                                                                                         aGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGt                                                                <  1:26906/146‑1 (MQ=255)
                                                                                          gCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATcc                                                                  <  3:551167/143‑1 (MQ=255)
                                                                                            cTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAAt                                                                    >  1:211534/1‑139 (MQ=255)
                                                                                                 tgctgTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAACCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATaaa                                                     >  3:952366/1‑149 (MQ=255)
                                                                                                    tgTACTCCGCCGGATATCTCTATTGTGATGCCATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGaa                                                                     <  2:430272/130‑1 (MQ=255)
                                                                                                    tgTACTCCGCCGGATATCTCTATTATGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGaa                                                                     >  1:430272/1‑130 (MQ=255)
                                                                                                     gTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCAt                                                        <  3:1271882/142‑1 (MQ=255)
                                                                                                        cTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGTCAGGTGGTGTGAATCCGTTGACGCATAAACGCGttc                                              <  4:1387786/149‑1 (MQ=255)
                                                                                                           cgccgGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGttcttc                                           <  1:1163861/149‑1 (MQ=255)
                                                                                                           cgccgGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGttcttc                                           <  2:717064/149‑1 (MQ=255)
                                                                                                             ccgGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGAc                                                            <  4:250209/130‑1 (MQ=255)
                                                                                                             ccgGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGAc                                                            >  3:250209/1‑130 (MQ=255)
                                                                                                               ggATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGc                                       >  3:572731/1‑149 (MQ=255)
                                                                                                                  tatCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGa                                    <  3:16991/149‑1 (MQ=255)
                                                                                                                   atCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGAc                                   >  4:395436/1‑149 (MQ=255)
                                                                                                                      tctATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGa                                    >  2:1210095/1‑145 (MQ=255)
                                                                                                                      tctATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAAc                                >  3:1220577/1‑149 (MQ=255)
                                                                                                                      tctATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAAc                                >  3:921476/1‑149 (MQ=255)
                                                                                                                      tctATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAAc                                >  2:22383/1‑149 (MQ=255)
                                                                                                                      tctATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAAc                                >  3:864422/1‑149 (MQ=255)
                                                                                                                        tATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGt                              <  3:49399/149‑1 (MQ=255)
                                                                                                                          ttGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGc                            >  3:1137483/1‑149 (MQ=255)
                                                                                                                              gATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAgg                                        <  1:284872/133‑1 (MQ=255)
                                                                                                                              gATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAgg                                        >  2:284872/1‑133 (MQ=255)
                                                                                                                                tGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGgtgt                                                                        >  2:790249/1‑99 (MQ=255)
                                                                                                                                tGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGgtgt                                                                        <  1:790249/99‑1 (MQ=255)
                                                                                                                                tGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACa                      >  2:1437346/1‑149 (MQ=255)
                                                                                                                                 gCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACAt                     >  3:743248/1‑149 (MQ=255)
                                                                                                                                   aaTGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTc                   <  1:695131/149‑1 (MQ=255)
                                                                                                                                    aTGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGc                                                                                                         <  1:953392/62‑1 (MQ=255)
                                                                                                                                    aTGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCtt                                                                                                  <  4:1220269/69‑1 (MQ=255)
                                                                                                                                     tGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTg                 >  1:934298/1‑149 (MQ=255)
                                                                                                                                      ggAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAATGCTTg                                                                                                 <  4:1250198/68‑1 (MQ=255)
                                                                                                                                      ggAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTg                                                                                                 >  3:1250198/1‑68 (MQ=255)
                                                                                                                                      ggAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAggtggt                                                                          >  1:182010/1‑91 (MQ=255)
                                                                                                                                            cTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGaaa          >  2:518157/1‑149 (MQ=255)
                                                                                                                                            cTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGaaa          <  2:191984/149‑1 (MQ=255)
                                                                                                                                             tgtgACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAat         >  2:1019336/1‑149 (MQ=255)
                                                                                                                                             actgACGGGTATACACGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGAc                                                            <  1:904666/96‑1 (MQ=255)
                                                                                                                                             actgACGGGTATACACGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGAc                                                            >  2:904666/3‑98 (MQ=255)
                                                                                                                                               tgACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCATCGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACTCATAAACGCGTTCTTCAGGCCGACAACGTGCCATACATTCTGGCCGAAatga       <  4:921476/149‑1 (MQ=255)
                                                                                                                                               tgACGGGTATACACGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGAc                                                            >  3:624298/1‑96 (MQ=255)
                                                                                                                                               tgACGGGTATACACGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAatga       >  2:560485/1‑149 (MQ=255)
                                                                                                                                                gACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAatgat      <  2:934298/149‑1 (MQ=255)
                                                                                                                                                gACGGGTATACACGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAAcgcg                                                 >  1:568310/1‑106 (MQ=255)
                                                                                                                                                 aCGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGa                                                                                           >  4:1201133/1‑63 (MQ=255)
                                                                                                                                                 aCGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGa                                                                                           <  3:1201133/63‑1 (MQ=255)
                                                                                                                                                 aCGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAatgatg     <  4:1137483/149‑1 (MQ=255)
                                                                                                                                                  cGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAatgatga    <  4:572731/149‑1 (MQ=255)
                                                                                                                                                    tgtATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAatgatgatg  >  4:1199519/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCCAACAGCAACTGGCTGGCGAGCAGGTAGCGCTCTCTGACGAAGTCAACCAGTCGGAACAAACAACCAACTTCCATAACCGGGCCATAGCCTGGCTGCTGTACTCCGCCGGATATCTCTATTGTGATGCAATGGAAGCCTGTGACGTGTATACCCGTCAGTGCTCCACGCTCCTCAATACTATTGAACTGGCAACGCTTGGCGCGACGCTGGCGGCAGGTGGTGTGAATCCGTTGACGCATAAACGCGTTCTTCAGGCCGACAACGTGCCGTACATTCTGGCCGAAATGATGATG  >  NC_000913/512064‑512360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: