Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 594,950 T→G 6.6% H165P (CAT→CCT)  cusR ← response regulator in two‑component regulatory system with CusS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913594,9500TG 6.6% 492.8 / 19.9 135H165P (CAT→CCT) cusRresponse regulator in two‑component regulatory system with CusS
Reads supporting (aligned to +/- strand):  ref base T (62/64);  new base G (5/4);  total (67/68)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 135 total aligned reads displayed.

TCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGC  >  NC_000913/594807‑595093
                                                                                                                                               |                                                                                                                                               
tCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCga                                                                                                                                            >  2:887052/1‑149 (MQ=255)
 cTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaa                                                                                                                                           <  1:887052/149‑1 (MQ=255)
  tGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                          <  1:1356376/149‑1 (MQ=255)
  tGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaag                                                                                                                                          <  3:1055695/149‑1 (MQ=255)
  tGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaag                                                                                                                                          >  4:1055695/1‑149 (MQ=255)
     aTTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaa                                                                                                                                       <  1:608928/149‑1 (MQ=255)
      ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaag                                                                                                                                      <  3:253274/149‑1 (MQ=255)
        aGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                    <  4:393155/149‑1 (MQ=255)
                 tCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCGTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCcagcag                                                                                                                           >  3:637369/1‑149 (MQ=255)
                    aaGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGt                                                                                                                        >  4:679301/1‑149 (MQ=255)
                          ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActc                                                                                                                  >  2:989145/1‑149 (MQ=255)
                          ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActc                                                                                                                  >  2:430554/1‑149 (MQ=255)
                          ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActc                                                                                                                  >  4:497117/1‑149 (MQ=255)
                          ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActc                                                                                                                  <  1:430554/149‑1 (MQ=255)
                                 ttttGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                           <  3:1004658/149‑1 (MQ=255)
                                        gcgcAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCACCAGAGTAAACTCTTTACTGGTCAAAg                                                                                                    <  4:1175802/149‑1 (MQ=255)
                                         cgcAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaag                                                                                                                                      <  3:1122927/114‑1 (MQ=255)
                                            aGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                <  3:497117/149‑1 (MQ=255)
                                             gCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTaaa                                                                                                                     >  1:360419/1‑127 (MQ=255)
                                               cGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                    <  2:84202/110‑1 (MQ=255)
                                               cGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                    >  1:84202/1‑110 (MQ=255)
                                               cGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTa                                                                                                              <  3:681308/132‑1 (MQ=255)
                                               cGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTa                                                                                                              >  4:681308/1‑132 (MQ=255)
                                                gCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagtgc                                                                                                                                       <  3:931932/106‑4 (MQ=255)
                                                    cACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGc                                                                                        <  2:1110221/149‑1 (MQ=255)
                                                    cACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGc                                                                                        >  2:1269548/1‑149 (MQ=255)
                                                     aCCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTc                                                                                                                                 >  3:196865/1‑107 (MQ=255)
                                                     aCCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTc                                                                                                                                 <  4:196865/107‑1 (MQ=255)
                                                      ccGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGa                                                                                                 >  1:791434/1‑138 (MQ=255)
                                                         ccACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTg                                                                                   >  2:293288/1‑149 (MQ=255)
                                                           acaTCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTa                                                                                                              >  3:352236/1‑120 (MQ=255)
                                                            caTCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCgg                                                                                <  2:485166/149‑1 (MQ=255)
                                                             gtCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCTAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                          >  2:1239805/2‑106 (MQ=255)
                                                              tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaaa                                                                                                     <  4:227393/126‑1 (MQ=255)
                                                              tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaaa                                                                                                     >  3:227393/1‑126 (MQ=255)
                                                                aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                          >  3:521215/1‑87 (MQ=255)
                                                                aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTcc                                                                                                                                <  4:568241/97‑1 (MQ=255)
                                                                aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTcc                                                                                                                                >  3:568241/1‑97 (MQ=255)
                                                                    gCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACttt                                                                        >  1:1036483/1‑149 (MQ=255)
                                                                     cATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagtg                                                                                                                                        >  2:795287/1‑82 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaa                                                                                                                                       <  2:380883/84‑1 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaa                                                                                                                                       >  1:380883/1‑84 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTc                                                                                                        >  4:534498/1‑115 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                      >  3:1077222/1‑149 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                      <  2:748800/149‑1 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                      <  1:1269548/149‑1 (MQ=255)
                                                                      aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                      <  1:989145/149‑1 (MQ=255)
                                                                             tCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGAc                                                               <  4:637369/149‑1 (MQ=255)
                                                                                 tGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACgaga                                                           <  1:1104718/149‑1 (MQ=255)
                                                                                 tGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACgaga                                                           <  4:1077222/149‑1 (MQ=255)
                                                                                  gTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCcagcagt                                                                                                                          >  4:875300/1‑84 (MQ=255)
                                                                                   tCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                           <  2:776012/99‑1 (MQ=255)
                                                                                   tCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                           <  3:321519/99‑1 (MQ=255)
                                                                                   tCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                           >  1:776012/1‑99 (MQ=255)
                                                                                   tCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                           >  4:321519/1‑99 (MQ=255)
                                                                                   tCAAAATTCATGTCCCATACCTGCGAGACGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATc                                                         >  3:1302645/1‑149 (MQ=255)
                                                                                     aaaaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGa                                                       >  2:287810/1‑149 (MQ=255)
                                                                                      aaaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGAc                                                      >  4:533043/1‑149 (MQ=255)
                                                                                       aaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGAcc                                                     <  2:1036483/149‑1 (MQ=255)
                                                                                        aTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCa                                                    >  4:281496/1‑149 (MQ=255)
                                                                                         ttCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAg                                                                                                    >  3:834240/1‑100 (MQ=255)
                                                                                          tCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATc                                                  <  2:945076/149‑1 (MQ=255)
                                                                                               tCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCg                                                                                      <  4:644887/108‑1 (MQ=255)
                                                                                               tCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCg                                                                                      >  3:644887/1‑108 (MQ=255)
                                                                                               tCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATc                                             <  3:281496/149‑1 (MQ=255)
                                                                                                 ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaa                                                                                                                                       <  1:241668/57‑1 (MQ=255)
                                                                                                  cATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCgg                                                                                >  3:57320/1‑111 (MQ=255)
                                                                                                    tACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCaa                                        >  2:38469/1‑149 (MQ=255)
                                                                                                     aCCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATc                                             >  1:328864/1‑143 (MQ=255)
                                                                                                     aCCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATc                                             <  2:328864/143‑1 (MQ=255)
                                                                                                         gCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCg                                                                     <  4:602364/115‑1 (MQ=255)
                                                                                                           gAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGcgcg                                                                                           <  1:592187/91‑1 (MQ=255)
                                                                                                           gAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGcgcg                                                                                           >  2:592187/1‑91 (MQ=255)
                                                                                                             ggCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACt                               <  4:20544/149‑1 (MQ=255)
                                                                                                              gCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTa                                                                                                              <  2:1346655/69‑1 (MQ=255)
                                                                                                               cGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGAc                                                               >  1:1301174/1‑115 (MQ=255)
                                                                                                               cGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGAc                                                               <  2:1301174/115‑1 (MQ=255)
                                                                                                               cGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGa                             <  4:1302645/149‑1 (MQ=255)
                                                                                                               cGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGa                             <  3:533043/149‑1 (MQ=255)
                                                                                                                gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGAc                            >  1:53307/1‑149 (MQ=255)
                                                                                                                   aaGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACttt                         <  2:53307/149‑1 (MQ=255)
                                                                                                                     gcgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCt                                                                  <  4:880605/106‑1 (MQ=255)
                                                                                                                     gcgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCt                                                                  >  3:880605/1‑106 (MQ=255)
                                                                                                                          cgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAAt                  <  1:1285965/149‑1 (MQ=255)
                                                                                                                            gggCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATc                                                  >  3:1411005/1‑115 (MQ=255)
                                                                                                                             ggCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGt                                                                                          >  1:157178/1‑74 (MQ=255)
                                                                                                                             ggCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGt                                                                                          <  2:157178/74‑1 (MQ=255)
                                                                                                                              gcagcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCAcc              >  3:601904/1‑149 (MQ=255)
                                                                                                                                 gcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCAccg             >  1:463957/1‑147 (MQ=255)
                                                                                                                                  tccTTCGCCATCAGGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCAccgccg          >  1:716803/3‑149 (MQ=255)
                                                                                                                                     ttCGCCATCAGGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGa                      >  4:7527/1‑134 (MQ=255)
                                                                                                                                     ttCGCCATCAGGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGa                      <  3:7527/134‑1 (MQ=255)
                                                                                                                                         cccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                      <  1:897536/82‑1 (MQ=255)
                                                                                                                                         cccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGAcc                                                     >  4:1034699/1‑99 (MQ=255)
                                                                                                                                          ccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGAt                                                          <  2:1089034/93‑1 (MQ=255)
                                                                                                                                          ccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGAt                                                          >  1:1089034/1‑93 (MQ=255)
                                                                                                                                          ccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCgcgc  >  4:1062566/1‑149 (MQ=255)
                                                                                                                                            tGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                <  4:319085/53‑1 (MQ=255)
                                                                                                                                            tGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATc                                             <  4:379847/104‑1 (MQ=255)
                                                                                                                                            tGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGa                      >  3:67414/1‑127 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGC  >  NC_000913/594807‑595093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: