Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 619,419 G→A 9.4% L261F (CTC→TTC)  fepC ← ferrienterobactin ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913619,4190GA 9.4% 251.1 / 11.7 84L261F (CTC→TTC) fepCferrienterobactin ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (39/37);  new base A (3/5);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAATGGCTG  >  NC_000913/619284‑619567
                                                                                                                                       |                                                                                                                                                    
tttCCGCGTTGATCGGCGGGATGGTGGCTTGGGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGc                                                                                                                                                  <  3:1281595/140‑1 (MQ=255)
       gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcac                                                                                                                                                <  4:447668/135‑1 (MQ=255)
       gTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcac                                                                                                                                                >  3:447668/1‑135 (MQ=255)
           aTCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGc                                                                                                                              >  3:442587/1‑149 (MQ=255)
                      ggtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGt                                                                                                                   >  1:614781/1‑149 (MQ=255)
                       gtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAAc                                                                                                                           >  4:480633/1‑140 (MQ=255)
                       gtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAAc                                                                                                                           <  3:480633/140‑1 (MQ=255)
                            ttGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCaa                                                                                                                <  2:511826/146‑1 (MQ=255)
                            ttGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCaa                                                                                                                >  1:511826/1‑146 (MQ=255)
                            ttGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGa                                                                                                             >  2:331839/1‑149 (MQ=255)
                            ttGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGa                                                                                                             >  1:43905/1‑149 (MQ=255)
                                 gtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATg                                                                                                        <  4:442587/149‑1 (MQ=255)
                                       tGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAGCTGGATCGTCAATGATCATGCAgcgc                                                                                                  >  2:1003916/1‑149 (MQ=255)
                                       tGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgcgc                                                                                                  >  4:859530/1‑149 (MQ=255)
                                          tGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGa                                                                                               <  1:331839/149‑1 (MQ=255)
                                            ttGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGca                                                                                                                                                 >  3:313367/1‑97 (MQ=255)
                                            ttGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGca                                                                                                                                                 <  4:313367/97‑1 (MQ=255)
                                                              tCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTc                                                                                                                  >  1:500763/1‑110 (MQ=255)
                                                              tCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTc                                                                                                                  <  2:500763/110‑1 (MQ=255)
                                                              tCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAg                                                                           >  4:719245/1‑149 (MQ=255)
                                                               ccAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCAACACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGt                                                                          <  2:614781/149‑1 (MQ=255)
                                                                cAGAAAACAGGATGCCATGATGGCGGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcac                                                                                                                                                <  3:227813/78‑1 (MQ=255)
                                                                cAGAAAACAGGATGCCATGATGGCGGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcac                                                                                                                                                >  4:227813/1‑78 (MQ=255)
                                                                cAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGtt                                                                         >  3:756708/1‑149 (MQ=255)
                                                                cAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCCCTCAATCAGtt                                                                         <  3:859530/149‑1 (MQ=255)
                                                                 aGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGa                                                                                               >  1:736130/1‑126 (MQ=255)
                                                                 aGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGa                                                                                               <  2:736130/126‑1 (MQ=255)
                                                                     aaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCaa                                                                               <  4:705709/138‑1 (MQ=255)
                                                                     aaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCaa                                                                               >  3:705709/1‑138 (MQ=255)
                                                                      aCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagcag                                                                   >  2:856685/1‑149 (MQ=255)
                                                                          gATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGAc                                                               >  4:905863/1‑149 (MQ=255)
                                                                          gATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGAc                                                               >  3:517443/1‑149 (MQ=255)
                                                                           aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACa                                                              <  4:756708/149‑1 (MQ=255)
                                                                            tGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                             >  2:1232739/1‑149 (MQ=255)
                                                                             gCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAt                                                            <  1:429529/149‑1 (MQ=255)
                                                                              ccATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     <  4:1167161/91‑1 (MQ=255)
                                                                              ccATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     >  3:1167161/1‑91 (MQ=255)
                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATg                                                                                                        <  4:122839/102‑1 (MQ=255)
                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATg                                                                                                        >  3:122839/1‑102 (MQ=255)
                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                                         >  4:417967/1‑149 (MQ=255)
                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                                         <  2:43905/149‑1 (MQ=255)
                                                                                atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                                         >  1:220610/1‑149 (MQ=255)
                                                                                 tgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     <  3:931096/88‑1 (MQ=255)
                                                                                 tgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        >  2:22792/1‑149 (MQ=255)
                                                                                 tgatgGCAGACCACTTAGTTTAACTACTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                                     >  4:931096/1‑88 (MQ=255)
                                                                                   atgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCtt                                                      <  3:417967/149‑1 (MQ=255)
                                                                                       cAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGgc                                                  <  3:719245/149‑1 (MQ=255)
                                                                                            cACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATaaa                                                                                        >  3:216584/1‑106 (MQ=255)
                                                                                            cACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATaaa                                                                                        <  4:216584/106‑1 (MQ=255)
                                                                                              cTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGtt                                                                         >  4:1200259/1‑119 (MQ=255)
                                                                                              cTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGtt                                                                         <  3:1200259/119‑1 (MQ=255)
                                                                                                tAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAGCTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGa                                         <  1:1003916/149‑1 (MQ=255)
                                                                                                 aGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAg                                        <  1:22792/149‑1 (MQ=255)
                                                                                                  gTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGc                                       <  3:25793/149‑1 (MQ=255)
                                                                                                   tttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGt                                                                  <  1:645073/121‑1 (MQ=255)
                                                                                                   tttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGt                                                                  >  2:645073/1‑121 (MQ=255)
                                                                                                     tAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGa                                                                                                                       >  3:557375/1‑66 (MQ=255)
                                                                                                     tAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGa                                                                                                                       <  4:557375/66‑1 (MQ=255)
                                                                                                     tAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaac                                    <  2:220610/149‑1 (MQ=255)
                                                                                                       aCTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATc                                                                                                           >  4:250466/1‑76 (MQ=255)
                                                                                                       aCTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATc                                                                                                           <  3:250466/76‑1 (MQ=255)
                                                                                                           tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTc                                                                        <  4:101452/107‑1 (MQ=255)
                                                                                                           tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGa                                                                >  4:1007921/1‑115 (MQ=255)
                                                                                                           tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGa                                                                <  3:1007921/115‑1 (MQ=255)
                                                                                                           tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                              >  1:308312/1‑149 (MQ=255)
                                                                                                           tttGCGGTTGAAGGAGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTc                                                                        >  3:101452/1‑107 (MQ=255)
                                                                                                              gCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttccc                           >  3:1348779/1‑149 (MQ=255)
                                                                                                                 gTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                              >  1:101789/1‑143 (MQ=255)
                                                                                                                 gTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                              <  2:101789/143‑1 (MQ=255)
                                                                                                                   tGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttccc                      <  1:1232739/149‑1 (MQ=255)
                                                                                                                    gAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCg                     <  2:308312/149‑1 (MQ=255)
                                                                                                                       ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        >  3:52093/1‑111 (MQ=255)
                                                                                                                       ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                                        <  4:52093/111‑1 (MQ=255)
                                                                                                                       ggTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaa                  <  4:517443/149‑1 (MQ=255)
                                                                                                                        gTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                                         <  3:354389/109‑1 (MQ=255)
                                                                                                                        gTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                                         >  4:354389/1‑109 (MQ=255)
                                                                                                                          gCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTc                                                                        <  3:1054987/92‑1 (MQ=255)
                                                                                                                            cGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCAt                                                                                           <  4:1360002/71‑1 (MQ=255)
                                                                                                                            cGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCAt                                                                                           >  3:1360002/1‑71 (MQ=255)
                                                                                                                              ttCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAAt                                                                              <  2:1262545/82‑1 (MQ=255)
                                                                                                                              ttCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAAt                                                                              >  1:1262545/1‑82 (MQ=255)
                                                                                                                                 gTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttc                             <  4:446975/128‑1 (MQ=255)
                                                                                                                                 gTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttc                             >  3:446975/1‑128 (MQ=255)
                                                                                                                                    cGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtgg     >  2:799661/1‑149 (MQ=255)
                                                                                                                                       gCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAAtggctg  >  2:1209115/1‑149 (MQ=255)
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TTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAATCAAATGGCTG  >  NC_000913/619284‑619567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: