Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 654,560 A→C 9.6% intergenic (+18/+23) citB → / ← dcuC response regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,5600AC 9.6% 318.4 / 22.2 94intergenic (+18/+23)citB/dcuCresponse regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base A (39/46);  new base C (3/6);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCG  >  NC_000913/654414‑654707
                                                                                                                                                  |                                                                                                                                                   
cAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGaaa                                                                                                                                                   <  3:102783/149‑1 (MQ=255)
 aGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                                                                  <  1:616513/149‑1 (MQ=255)
   gCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                <  4:1089408/149‑1 (MQ=255)
     aCTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGc                                                                                                                                              <  1:927489/149‑1 (MQ=255)
        gACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGTAAACTTGCCTc                                                                                                                                           <  1:1084798/149‑1 (MQ=255)
            aTCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAATGCCGGAGGCAAGTTTCCTCCGGc                                                                                                                                       <  3:1295320/149‑1 (MQ=255)
             tCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                                                                      >  2:726629/1‑149 (MQ=255)
              cAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCAt                                                                                                                                     <  1:722104/149‑1 (MQ=255)
                 ccGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                                                                  >  4:1299120/1‑149 (MQ=255)
                   gCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTa                                                                                                                                >  1:1425126/1‑149 (MQ=255)
                   gCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTa                                                                                                                                >  2:535195/1‑149 (MQ=255)
                     accacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAtt                                                                                                                              <  4:1248140/149‑1 (MQ=255)
                       cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                <  1:956626/129‑1 (MQ=255)
                       cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                >  2:956626/1‑129 (MQ=255)
                                gCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgt                                                                                                                   >  3:124603/1‑149 (MQ=255)
                                  gTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGa                                                                                                                 <  4:124603/149‑1 (MQ=255)
                                      tCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCgg                                                                                                                                        <  3:138607/122‑1 (MQ=255)
                                      tCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCgg                                                                                                                                        >  4:138607/1‑122 (MQ=255)
                                      tCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgc                                                                                                             <  2:1425126/149‑1 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGtt                                                                                                                                                <  1:317500/112‑1 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGtt                                                                                                                                                <  2:422737/112‑1 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                                                                      <  3:1096172/122‑1 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                                                                      >  4:1096172/1‑122 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                           >  2:891294/1‑149 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                           >  2:168648/1‑149 (MQ=255)
                                         tGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgc                                                                                                          >  2:935924/1‑149 (MQ=255)
                                          gAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                <  3:1259355/110‑1 (MQ=255)
                                          gAATATTGCACCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                >  4:1259355/1‑110 (MQ=255)
                                             tatTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgc                                                                                                          >  3:1415069/1‑145 (MQ=255)
                                             tatTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgc                                                                                                          <  4:1415069/145‑1 (MQ=255)
                                               ttGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                >  3:373265/1‑105 (MQ=255)
                                               ttGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                <  4:373265/105‑1 (MQ=255)
                                                   gcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCt                                                                                                <  3:1299120/149‑1 (MQ=255)
                                                    ccAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                                                               <  1:935924/149‑1 (MQ=255)
                                                     cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACATTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                              <  1:891294/149‑1 (MQ=255)
                                                          gccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCa                                                                                         <  1:535195/149‑1 (MQ=255)
                                                                 gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                                >  2:51429/1‑87 (MQ=255)
                                                                 gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGAGAAAGCCGGAGGAAACtt                                                                                                                                                <  1:51429/87‑1 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                                                                  <  1:892512/77‑1 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                                                                  >  2:892512/1‑77 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAtt                                                                                                                              <  1:152977/97‑1 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAtt                                                                                                                              >  2:152977/1‑97 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                                                                          >  4:488723/1‑133 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                                                                          <  3:488723/133‑1 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                                                                          <  1:726629/149‑1 (MQ=255)
                                                                         cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                                                                          <  1:168648/149‑1 (MQ=255)
                                                                          tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                         >  4:38863/1‑149 (MQ=255)
                                                                            aaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                                                                   >  1:529734/1‑89 (MQ=255)
                                                                            aaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                                                                   <  2:529734/89‑1 (MQ=255)
                                                                             aaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCt                                                                                                <  4:443875/123‑1 (MQ=255)
                                                                             aaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCt                                                                                                >  3:443875/1‑123 (MQ=255)
                                                                               ttGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGcc                                                                                                      <  4:137670/115‑1 (MQ=255)
                                                                               ttGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGcc                                                                                                      >  3:137670/1‑115 (MQ=255)
                                                                                    cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                                                           >  2:1347091/1‑89 (MQ=255)
                                                                                    cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                                                           <  1:1347091/89‑1 (MQ=255)
                                                                                     aCGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                              >  3:735885/1‑117 (MQ=255)
                                                                                     aCGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                              <  4:735885/117‑1 (MQ=255)
                                                                                     aCGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                                                              <  3:38863/149‑1 (MQ=255)
                                                                                        gCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCTGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGa                                                                       <  3:1220641/137‑1 (MQ=255)
                                                                                        gCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCTGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGa                                                                       >  4:1220641/1‑137 (MQ=255)
                                                                                           aaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                        >  3:716543/1‑69 (MQ=255)
                                                                                           aaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                        <  4:716543/69‑1 (MQ=255)
                                                                                              ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAt                                                                                                                               <  1:446638/75‑1 (MQ=255)
                                                                                              ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAt                                                                                                                               >  2:446638/1‑75 (MQ=255)
                                                                                              ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaaccaa                                                     >  1:46182/1‑149 (MQ=255)
                                                                                                ggCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaaca                                                   >  2:675320/1‑149 (MQ=255)
                                                                                                ggCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaaca                                                   >  4:409293/1‑149 (MQ=255)
                                                                                                    gACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                         <  2:481521/123‑1 (MQ=255)
                                                                                                    gACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                         >  1:481521/1‑123 (MQ=255)
                                                                                                     aCCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCAc                                              <  1:675320/149‑1 (MQ=255)
                                                                                                       cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                              >  3:1425260/1‑99 (MQ=255)
                                                                                                       cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                              <  4:1425260/99‑1 (MQ=255)
                                                                                                       cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                                                              >  2:285923/1‑131 (MQ=255)
                                                                                                       cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                                                              <  1:285923/131‑1 (MQ=255)
                                                                                                             gCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACtt                                                                                                                         <  2:810444/66‑1 (MQ=255)
                                                                                                             gCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACtt                                                                                                                         >  1:810444/1‑66 (MQ=255)
                                                                                                               atatACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTg                                                                                >  3:564455/1‑105 (MQ=255)
                                                                                                               atatACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTg                                                                                <  4:564455/105‑1 (MQ=255)
                                                                                                                    ccACAGTGGGTGACATGATAAAGCCGGAGTAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                                                                 >  1:1147818/1‑83 (MQ=255)
                                                                                                                    ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                                                                 <  2:1147818/83‑1 (MQ=255)
                                                                                                                          tGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                         <  2:46182/149‑1 (MQ=255)
                                                                                                                                 cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGt                                  <  2:967873/133‑1 (MQ=255)
                                                                                                                                 cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGt                                  >  1:967873/1‑133 (MQ=255)
                                                                                                                                  aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctct                                                                                 >  2:32610/1‑85 (MQ=255)
                                                                                                                                  aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctct                                                                                 <  1:32610/85‑1 (MQ=255)
                                                                                                                                      tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACgg             <  1:1366529/149‑1 (MQ=255)
                                                                                                                                       aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTa                                                                              <  3:1305536/83‑1 (MQ=255)
                                                                                                                                       aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTa                                                                              >  4:1305536/1‑83 (MQ=255)
                                                                                                                                         aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCga          <  3:409293/149‑1 (MQ=255)
                                                                                                                                          gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCa                                                                                      >  2:785716/1‑72 (MQ=38)
                                                                                                                                          gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCa                                                                                      <  1:785716/72‑1 (MQ=38)
                                                                                                                                          gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                                                                   >  4:888955/1‑91 (MQ=255)
                                                                                                                                          gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                                                                   <  3:888955/91‑1 (MQ=255)
                                                                                                                                           ccGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTa                                         <  1:903846/116‑1 (MQ=255)
                                                                                                                                           ccGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTa                                         >  2:903846/1‑116 (MQ=255)
                                                                                                                                               aggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCtt    >  3:308729/1‑149 (MQ=255)
                                                                                                                                                 gaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCg  >  2:1194208/1‑149 (MQ=255)
                                                                                                                                                  aaaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                                                                  >  2:1424545/1‑68 (MQ=255)
                                                                                                                                                  aaaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                                                                  <  1:1424545/68‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCG  >  NC_000913/654414‑654707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: