Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 691,390 G→C 5.9% A132G (GCC→GGC)  ybeX ← putative ion transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913691,3900GC 5.9% 788.9 / 29.1 220A132G (GCC→GGC) ybeXputative ion transport
Reads supporting (aligned to +/- strand):  ref base G (108/99);  new base C (7/6);  total (115/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 228 total aligned reads displayed.

AATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTGGTTG  >  NC_000913/691242‑691533
                                                                                                                                                    |                                                                                                                                               
aaTGGTCACCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAgg                                                                                                                                                 >  2:266710/1‑149 (MQ=255)
       aCCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGc                                                                                                                                          >  1:1248459/1‑149 (MQ=255)
       aCCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGc                                                                                                                                          >  1:986464/1‑149 (MQ=255)
        ccAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCa                                                                                                                                         >  3:218777/1‑149 (MQ=255)
              cGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCAtg                                                                                                                                       <  2:787138/145‑2 (MQ=255)
              cGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGCCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTg                                                                                                                                   >  3:723199/1‑149 (MQ=255)
                gAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAt                                                                                                                                        <  2:614258/142‑1 (MQ=255)
                gAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAt                                                                                                                                        >  1:614258/1‑142 (MQ=255)
                     cccACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCa                                                                                                                                         >  1:154447/1‑136 (MQ=255)
                      ccACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATaaa                                                                                                                           >  3:485810/1‑149 (MQ=255)
                        aCCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCa                                                                                                                                         >  1:239208/1‑133 (MQ=255)
                           gAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCGTCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgca                                                                                                                      <  2:843688/149‑1 (MQ=255)
                              ttCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgc                                                                                                                                  >  2:1259389/1‑134 (MQ=255)
                              ttCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgc                                                                                                                                  <  1:1259389/134‑1 (MQ=255)
                               tCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCaa                                                                                                                  <  4:485810/149‑1 (MQ=255)
                                 gTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCtt                                                                                                                                              >  1:161442/1‑119 (MQ=255)
                                  tCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgc                                                                                                                                  <  1:1360840/130‑1 (MQ=255)
                                  tCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgc                                                                                                                                  >  2:1360840/1‑130 (MQ=255)
                                    aaTAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgc                                                                                                                                  >  4:897801/1‑128 (MQ=255)
                                    aaTAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCtt                                                                                                             >  4:1127215/1‑149 (MQ=255)
                                    aaTAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCGGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgc                                                                                                                                  <  3:897801/128‑1 (MQ=255)
                                         cGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGcc                                                                                                        <  4:94331/149‑1 (MQ=255)
                                         cGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGcc                                                                                                        <  1:1127966/149‑1 (MQ=255)
                                           aTCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACgg                                                                                                                        >  2:835778/1‑131 (MQ=255)
                                             cGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCa                                                                                                    <  3:317316/149‑1 (MQ=255)
                                              gCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAg                                                                                                   >  3:1212835/1‑149 (MQ=255)
                                                         aCGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                                                                        <  2:1248459/149‑1 (MQ=255)
                                                              gagaGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGa                                                                                   <  4:72029/149‑1 (MQ=255)
                                                               atgtcttgatgtcatcatcgagtccgcccactcacgtttcccggtgattagcgaagacaaagatcacattgaagggattCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGa                                                                                                  <  3:783771‑M1/54‑1 (MQ=255)
                                                               atgtcttgatgtcatcatcgagtccgcccactcacgtttcccggtgattagcgaagacaaagatcacattgaagggattCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGa                                                                                                  >  4:783771‑M1/80‑133 (MQ=255)
                                                                gagCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATc                                                                                 <  1:1199995/149‑1 (MQ=255)
                                                                gagCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATc                                                                                 <  2:155167/149‑1 (MQ=255)
                                                                           tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAAc                                                                                                                          >  4:262042/1‑97 (MQ=255)
                                                                           tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTg                                                                             <  4:1269329/142‑1 (MQ=255)
                                                                           tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTg                                                                             >  3:1269329/1‑142 (MQ=255)
                                                                           tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGc                                                                      >  2:385124/1‑149 (MQ=255)
                                                                           tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGc                                                                      >  3:1189333/1‑149 (MQ=255)
                                                                             tCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTa                                                                    >  2:947843/1‑149 (MQ=255)
                                                                               aGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCtt                                                                         <  3:982960/142‑1 (MQ=255)
                                                                                gCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATaaa                                                                                                                           <  3:614211/91‑1 (MQ=255)
                                                                                gCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATaaa                                                                                                                           >  4:614211/1‑91 (MQ=255)
                                                                                  aTCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTc                                                                        <  2:166040/140‑1 (MQ=255)
                                                                                   tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCt                                                                                            <  1:1086689/119‑1 (MQ=255)
                                                                                   tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCt                                                                                            <  1:1018276/119‑1 (MQ=255)
                                                                                   tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCttt                                                                              >  3:1318290/1‑133 (MQ=255)
                                                                                   tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCttt                                                                              <  4:1318290/133‑1 (MQ=255)
                                                                                   tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAcc                                                              >  2:416174/1‑149 (MQ=255)
                                                                                   tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAcc                                                              >  3:101678/1‑149 (MQ=255)
                                                                                    ccGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCTCTTCAATGTGATCTTTGTCTTCGCTAAACACCg                                                             <  1:414489/149‑1 (MQ=255)
                                                                                          tACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                                                            <  2:670866/64‑1 (MQ=255)
                                                                                          tACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                                                            >  1:670866/1‑64 (MQ=255)
                                                                                          tACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgcgc                                                                                                                                <  3:1346576/76‑1 (MQ=255)
                                                                                          tACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAAc                                                       >  4:415177/1‑149 (MQ=255)
                                                                                          tACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAAc                                                       >  1:841251/1‑149 (MQ=255)
                                                                                             gcgcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                                                            >  2:1374695/1‑61 (MQ=255)
                                                                                             gcgcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                                                            <  1:1374695/61‑1 (MQ=255)
                                                                                              cgcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTc                                                                        >  4:1229748/1‑128 (MQ=255)
                                                                                              cgcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGa                                                   <  4:1189333/149‑1 (MQ=255)
                                                                                               gcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                                                            <  2:413147/59‑1 (MQ=255)
                                                                                               gcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg                                                  <  4:1212835/149‑1 (MQ=255)
                                                                                                  tACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTgg                                               <  3:415177/149‑1 (MQ=255)
                                                                                                     tttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGt                                                                            <  4:1208013/117‑1 (MQ=255)
                                                                                                     tttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGt                                                                            >  3:1208013/1‑117 (MQ=255)
                                                                                                     tttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCg                                            >  1:793402/1‑149 (MQ=255)
                                                                                                      ttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCgg                                           >  2:895425/1‑149 (MQ=255)
                                                                                                             aCGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCgat                                    >  4:35917/1‑149 (MQ=255)
                                                                                                              cGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCtt                                                                                           >  4:1372269/1‑93 (MQ=255)
                                                                                                                 cAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGatgatg                                <  3:35917/149‑1 (MQ=255)
                                                                                                                  aaCCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGc                                                                      <  3:161819/110‑1 (MQ=255)
                                                                                                                              tAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGAt                                                                                  <  2:1090903/86‑1 (MQ=255)
                                                                                                                              tAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCttt                                                                              <  1:1158558/90‑1 (MQ=255)
                                                                                                                              tAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCCGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTc                                                                        <  1:411314/96‑1 (MQ=255)
                                                                                                                                    tttGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                                                                        >  3:820270/1‑74 (MQ=255)
                                                                                                                                       gTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTggg                                              >  1:536208/1‑113 (MQ=255)
                                                                                                                                       gTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTggg                                              <  2:536208/113‑1 (MQ=255)
                                                                                                                                        tCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGt         >  3:648928/1‑149 (MQ=255)
                                                                                                                                         ccATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTc        <  2:1072758/149‑1 (MQ=255)
                                                                                                                                          gaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCt       >  4:881876/2‑149 (MQ=255)
                                                                                                                                          cATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCgatga                                  >  3:1408023/1‑122 (MQ=255)
                                                                                                                                           aTGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCg                                                                       <  1:169022/84‑1 (MQ=255)
                                                                                                                                           aTGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACAt                            >  1:1067452/1‑127 (MQ=255)
                                                                                                                                           aTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCga                                     >  1:139426/1‑118 (MQ=255)
                                                                                                                                            tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTgg     >  4:645369/1‑149 (MQ=255)
                                                                                                                                            tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTgg     <  2:1414469/149‑1 (MQ=255)
                                                                                                                                            tGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtgtg                                                                                    <  2:104939/70‑1 (MQ=255)
                                                                                                                                             gCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACa                             <  3:678456/124‑1 (MQ=255)
                                                                                                                                             gCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACa                             >  4:678456/1‑124 (MQ=255)
                                                                                                                                              CTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTGGtt   >  1:895425‑M1/1‑149 (MQ=255)
                                                                                                                                               TGAAGGCTTCAGCGTCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTGGTTg  <  2:460615‑M1/149‑1 (MQ=255)
                                                                                                                                               TGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCgatga                                  <  4:217552‑M1/117‑1 (MQ=255)
                                                                                                                                               TGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCgatga                                  <  1:427263‑M1/117‑1 (MQ=255)
                                                                                                                                                GAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTaa                                                                   <  3:804018‑M1/83‑1 (MQ=255)
                                                                                                                                                GAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTaa                                                                   >  4:804018‑M1/1‑83 (MQ=255)
                                                                                                                                                 AAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCtt                                                                               <  3:1301255‑M1/70‑1 (MQ=255)
                                                                                                                                                 AAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCtt                                                                               >  4:1301255‑M1/1‑70 (MQ=255)
                                                                                                                                                 AAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCgg                                           <  2:1139040‑M1/106‑1 (MQ=255)
                                                                                                                                                 AAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATc                           >  4:1469407‑M1/1‑122 (MQ=255)
                                                                                                                                                   ggCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTGGt    >  3:732541/1‑143 (MQ=255)
                                                                                                                                                   ggCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTGGt    <  4:732541/143‑1 (MQ=255)
                                                                                                                                                    gCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAAGGTGAGTGGGCGGACTCgat                                    <  3:1215272/110‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGATGACATCAAGACATTCGTCCAGCGTCTGGTTG  >  NC_000913/691242‑691533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: