Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 745,361 T→C 7.3% F66S (TTT→TCT) ‡ ybgL → UPF0271 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913745,3610TC 7.3% 272.7 / 8.5 82F66S (TTT→TCT) ‡ybgLUPF0271 family protein
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base C (3/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

GCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAA  >  NC_000913/745213‑745509
                                                                                                                                                    |                                                                                                                                                    
gCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAAttt                                                                                                                                                      >  2:1071017/1‑149 (MQ=255)
gCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAAttt                                                                                                                                                      >  1:5675/1‑149 (MQ=255)
            aCGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgc                                                                                                                                          >  1:1071017‑M2/1‑144 (MQ=255)
                  gTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgcc                                                                                                                                         <  1:1147412‑M2/144‑7 (MQ=255)
                  gTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgcc                                                                                                                                         >  2:1147412‑M2/1‑138 (MQ=255)
                   tttCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAAtttt                                                                                                                                                     <  2:1401618/131‑1 (MQ=255)
                   tttCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAAtttt                                                                                                                                                     >  1:1401618/1‑131 (MQ=255)
                       ctctGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTgc                                                                                                                               >  2:1155151/1‑149 (MQ=255)
                        tctGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAAtttt                                                                                                                                                     >  1:1199633/1‑126 (MQ=255)
                        tctGCCAATATTGCCTGTGGATTTCAAGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGTGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGATTTTTCCCGACAGGGAAAAtttt                                                                                                                                                     <  2:1199633/126‑1 (MQ=255)
                            ccAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCa                                                                                                                          >  3:573996/1‑149 (MQ=255)
                            ccAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCa                                                                                                                          >  1:579640/1‑149 (MQ=255)
                                tatTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGaaa                                                                                                                      >  3:277959/1‑149 (MQ=255)
                                    gCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGt                                                                                                                  <  1:1155151/149‑1 (MQ=255)
                                         tgGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAg                                                                                                                                   >  4:491980/1‑127 (MQ=255)
                                         tgGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAg                                                                                                                                   <  3:491980/127‑1 (MQ=255)
                                         tgGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGaaa                                                                                                                      <  4:244812/140‑1 (MQ=255)
                                         tgGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGaaa                                                                                                                      >  3:244812/1‑140 (MQ=255)
                                         tgGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACg                                                                                                             >  3:1422963/1‑149 (MQ=255)
                                             tttCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCa                                                                                                         >  1:1228863/1‑149 (MQ=255)
                                                  tGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGc                                                                                                    >  2:927725/1‑149 (MQ=255)
                                                   gCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCt                                                                                                   <  2:1228863/149‑1 (MQ=255)
                                                     aGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGt                                                                                                 <  4:573996/149‑1 (MQ=255)
                                                     aGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGt                                                                                                 <  2:579640/149‑1 (MQ=255)
                                                       gCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTAt                                                                                               <  4:277959/149‑1 (MQ=255)
                                                              gcAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGaaa                                                                                                                      <  2:27887/119‑1 (MQ=255)
                                                              gcAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGaaa                                                                                                                      >  1:27887/1‑119 (MQ=255)
                                                                  aTCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGgcgc                                                                                    <  1:927725/149‑1 (MQ=255)
                                                                      tGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGt                                                                                                 <  2:252076/132‑1 (MQ=255)
                                                                      tGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTg                                                                                >  4:1161506/1‑149 (MQ=255)
                                                                      tGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATACAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGt                                                                                                 >  1:252076/1‑132 (MQ=255)
                                                                        cAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTgccgcc                                                                                                                           >  2:538496/1‑104 (MQ=255)
                                                                        cAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTgccgcc                                                                                                                           <  1:538496/104‑1 (MQ=255)
                                                                           gCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAAc                                                                           >  3:631352/1‑149 (MQ=255)
                                                                             ttGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTa                                                                                                               <  1:913272/111‑1 (MQ=255)
                                                                             ttGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTa                                                                                                               >  2:913272/1‑111 (MQ=255)
                                                                                     gtgAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCt                                                                                                                                 <  1:367293/85‑1 (MQ=255)
                                                                                     gtgAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCt                                                                                                                                 >  2:367293/1‑85 (MQ=255)
                                                                                     gtgAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGt                                                                 <  4:631352/149‑1 (MQ=255)
                                                                                        aaGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGt                                                                                                                  >  4:449358/1‑97 (MQ=255)
                                                                                        aaGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGt                                                                                                                  <  3:449358/97‑1 (MQ=255)
                                                                                        aaGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCt                                                                                                   <  3:897535/112‑1 (MQ=255)
                                                                                        aaGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCATTTACGCCCAGACGCt                                                                                                   >  4:897535/1‑112 (MQ=255)
                                                                                          gCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTg                                                                                                                                                    <  4:534074/61‑1 (MQ=255)
                                                                                          gCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTg                                                                                                                                                    >  3:534074/1‑61 (MQ=255)
                                                                                              tAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCt                                                                                                   >  4:867751/1‑106 (MQ=255)
                                                                                              tAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCt                                                                                                   <  3:867751/106‑1 (MQ=255)
                                                                                              tAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAggc                                                        >  1:608545/1‑149 (MQ=255)
                                                                                                        tgtCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGc                                              >  4:813651/1‑149 (MQ=255)
                                                                                                         gtCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCt                                                                                 >  2:788586/1‑113 (MQ=255)
                                                                                                         gtCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCt                                                                                 <  1:788586/113‑1 (MQ=255)
                                                                                                            gcgATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCa                                          >  4:1155959/1‑149 (MQ=255)
                                                                                                             cgATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCAt                                         <  4:1422963/149‑1 (MQ=255)
                                                                                                              gATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgc                                                                              <  3:1072575‑M2/111‑66 (MQ=255)
                                                                                                              gATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgc                                                                              >  4:1072575‑M2/1‑46 (MQ=255)
                                                                                                              gATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATg                                        >  1:719028/1‑149 (MQ=255)
                                                                                                                ttGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatccacaggcaatattggcagaggaaaccag                                      <  3:1271520‑M2/149‑106 (MQ=255)
                                                                                                                   gcgcTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGgcggcg                                                    >  3:204185/1‑132 (MQ=255)
                                                                                                                   gcgcTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGgcggcg                                                    <  4:204185/132‑1 (MQ=255)
                                                                                                                       tCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTTGCGCGCTGGCAACGATTGCCCGTGCGCAAGgcggcg                                                    >  1:28498/1‑128 (MQ=255)
                                                                                                                       tCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGgcggcg                                                    <  2:28498/128‑1 (MQ=255)
                                                                                                                         aCCCGAGTTGTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCAc                             >  1:1256484/1‑149 (MQ=255)
                                                                                                                           ccGAGTTTTCCCGACAGGGAAAATTCGGGTGgcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatc                                                                    >  2:626102‑M2/1‑32 (MQ=255)
                                                                                                                           ccGAGTTTTCCCGACAGGGAAAATTCGGGTGgcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatc                                                                    <  1:626102‑M2/108‑77 (MQ=255)
                                                                                                                           ccGAGTTTTCCCGACAGGGAAAATTCGGGTGgcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatc                                                                    <  1:909464‑M2/108‑77 (MQ=255)
                                                                                                                           ccGAGTTTTCCCGACAGGGAAAATTCGGGTGgcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatc                                                                    >  2:909464‑M2/1‑32 (MQ=255)
                                                                                                                            cGAGTTTTCCCGACAGGGAAAATTTTGGTCGCagcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatccacaggcaatattggcagaggaaaccagcgttaatagctc                          >  4:1271520‑M2/1‑32 (MQ=255)
                                                                                                                             gAGTTTTCCCGACAGGGAAAATTCGGGTGgcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatccacaggcaatattggcagaggaaaccagcgttaatag                               >  2:995043‑M2/1‑30 (MQ=255)
                                                                                                                             gAGTTTTCCCGACAGGGAAAATTCGGGTGgcgccaatcgcgacaccattttttattgcttcacgcacgcaagcctgcatgatttgcgcatcgcctgcatgaaatccacaggcaatattggcagaggaaaccagcgttaatag                               <  1:995043‑M2/143‑114 (MQ=255)
                                                                                                                                ttttCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCAc                             >  4:563290/1‑142 (MQ=255)
                                                                                                                                ttttCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCAc                             <  3:563290/142‑1 (MQ=255)
                                                                                                                                ttttCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCAtgt                      >  2:1283703/1‑149 (MQ=255)
                                                                                                                                    cccGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCAtgtt                     <  2:415819/146‑1 (MQ=255)
                                                                                                                                    cccGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCAtgtt                     >  1:415819/1‑146 (MQ=255)
                                                                                                                                    cccGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTa                  <  3:1155959/149‑1 (MQ=255)
                                                                                                                                     ccGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTAc                 >  1:610811/1‑149 (MQ=255)
                                                                                                                                          aGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCa            >  3:664912/1‑149 (MQ=255)
                                                                                                                                          aGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCa            >  1:872146/1‑149 (MQ=255)
                                                                                                                                           gggAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAg           <  2:719028/149‑1 (MQ=255)
                                                                                                                                            ggAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGgc                                                                                      <  2:267755/73‑1 (MQ=255)
                                                                                                                                            ggAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGgc                                                                                      >  1:267755/1‑73 (MQ=255)
                                                                                                                                            ggAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAAc              <  1:1123512/145‑1 (MQ=255)
                                                                                                                                            ggAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAAc              >  2:1123512/1‑145 (MQ=255)
                                                                                                                                             gAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATg                                               >  1:901987/1‑111 (MQ=255)
                                                                                                                                             gAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATg                                               <  2:901987/111‑1 (MQ=255)
                                                                                                                                             gAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAggc         >  3:847687/1‑149 (MQ=255)
                                                                                                                                               aaaTTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAggcgg       <  4:664912/149‑1 (MQ=255)
                                                                                                                                               aaaTTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAggcgg       <  3:813651/149‑1 (MQ=255)
                                                                                                                                                    ttGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGaaa  <  4:847687/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAA  >  NC_000913/745213‑745509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: