Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 111,016 T→C 22.2% intergenic (+32/‑28) secA → / → mutT preprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913111,0160TC22.2% 31.3 / 5.0 18intergenic (+32/‑28)secA/mutTpreprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base T (7/7);  new base C (2/2);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

CTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGT  >  NC_000913/110869‑111157
                                                                                                                                                   |                                                                                                                                             
ctGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTc                                                                                                                                              <  2:255962/149‑1 (MQ=255)
                                             tAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGcc                                                                                                                                        <  2:483706/110‑1 (MQ=255)
                                             tAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGcc                                                                                                                                        >  1:483706/1‑110 (MQ=255)
                                                        gATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAGAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCttt                                                                                                                                     <  1:1211758/102‑1 (MQ=255)
                                                        gATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCttt                                                                                                                                     >  2:1211758/1‑102 (MQ=255)
                                                                                    aaTACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGc                                                                                                          <  3:435922/101‑1 (MQ=255)
                                                                                    aaTACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGc                                                                                                          >  4:435922/1‑101 (MQ=255)
                                                                                                                aTAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTgcgc                                                                                                                                         <  3:65771/42‑1 (MQ=255)
                                                                                                                aTAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTgcgc                                                                                                                                         >  4:65771/1‑42 (MQ=255)
                                                                                                                              ttGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCg                                                                                               >  1:824747/1‑70 (MQ=255)
                                                                                                                              ttGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCg                                                                                               <  2:824747/70‑1 (MQ=255)
                                                                                                                                        aGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGcaca                               >  2:1129143/1‑124 (MQ=255)
                                                                                                                                        aGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGcaca                               <  1:1129143/124‑1 (MQ=255)
                                                                                                                                        aGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGa                         <  1:32333/130‑1 (MQ=255)
                                                                                                                                        aGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGa                         >  2:32333/1‑130 (MQ=255)
                                                                                                                                            gcAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGt  >  1:379858/1‑149 (MQ=255)
                                                                                                                                              aGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATc                                                              <  1:532907/87‑1 (MQ=255)
                                                                                                                                              aGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATc                                                              >  2:532907/1‑87 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGT  >  NC_000913/110869‑111157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: