Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 994,627 G→T 7.7% A69E (GCG→GAG)  ssuC ← aliphatic sulfonate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913994,6270GT 7.7% 319.2 / 12.9 91A69E (GCG→GAG) ssuCaliphatic sulfonate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (44/40);  new base T (3/4);  total (47/44)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AGCGGGATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCC  >  NC_000913/994489‑994775
                                                                                                                                          |                                                                                                                                                    
aGCGGGATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGa                                                                                                                                            <  3:1099235/149‑1 (MQ=255)
   gggATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCt                                                                                                                                         <  3:610598/149‑1 (MQ=255)
   gggATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCt                                                                                                                                         <  1:1346223/149‑1 (MQ=255)
   gggATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCt                                                                                                                                         <  3:342600/149‑1 (MQ=255)
     gATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGa                                                                                                                                       <  2:382910/149‑1 (MQ=255)
             ccAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGa                                                                                                                                       <  1:272108/141‑1 (MQ=255)
             ccAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGa                                                                                                                                       >  2:272108/1‑141 (MQ=255)
                   gCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         <  4:1384180/149‑1 (MQ=255)
                        aCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAg                                                                                                                    >  1:299747/1‑149 (MQ=255)
                               gcAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGAtt                                                                                                                                     >  2:749743/1‑124 (MQ=255)
                               gcAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGAtt                                                                                                                                     <  1:749743/125‑2 (MQ=255)
                                 aaCATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGAt                                                                                                                                      >  1:1185129/1‑122 (MQ=255)
                                 aaCATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGAt                                                                                                                                      <  2:1185129/122‑1 (MQ=255)
                                     tCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         >  3:130956/1‑131 (MQ=255)
                                     tCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         <  4:130956/131‑1 (MQ=255)
                                           ttGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGc                                                                                                 >  2:1282395/1‑149 (MQ=255)
                                           ttGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGc                                                                                                 >  2:543223/1‑149 (MQ=255)
                                              aGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAgcgc                                                                                                                                                     <  2:1318139/94‑1 (MQ=255)
                                              aGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAgcgc                                                                                                                                                     >  1:1318139/1‑94 (MQ=255)
                                                gTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgct                                                                                                                           <  1:630950/118‑1 (MQ=255)
                                                gTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgct                                                                                                                           >  2:630950/1‑118 (MQ=255)
                                                       gcagcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGcc                                                                                     >  4:994332/1‑149 (MQ=255)
                                                         agcCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGt                                                                                   <  2:299747/149‑1 (MQ=255)
                                                            cGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcac                                                                                <  1:1282395/149‑1 (MQ=255)
                                                              ttCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTc                                                                                              <  1:626824/133‑1 (MQ=255)
                                                              ttCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTc                                                                                              >  2:626824/1‑133 (MQ=255)
                                                                 tcCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTc                                                                           >  4:174295/1‑149 (MQ=255)
                                                                  ccccAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCt                                                                                                                                         <  2:465227/86‑1 (MQ=255)
                                                                  ccccAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCt                                                                                                                                         >  1:465227/1‑86 (MQ=255)
                                                                   cccAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCt                                                                         >  4:164339/1‑149 (MQ=255)
                                                                    ccAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGGAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCtt                                                                        >  1:1463326/1‑149 (MQ=255)
                                                                      aGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGGGCGTCCAGAACGCCGTCACCACTCCTTcc                                                                      >  4:1389380/1‑149 (MQ=255)
                                                                       gCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGAt                                                                                                                                      >  4:429978/1‑84 (MQ=255)
                                                                       gCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGAt                                                                                                                                      <  3:429978/84‑1 (MQ=255)
                                                                         gagaTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGt                                                                   <  1:543223/149‑1 (MQ=255)
                                                                           gaTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         >  2:1394830/1‑93 (MQ=255)
                                                                           gaTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGa                                                                 <  1:1415010/149‑1 (MQ=255)
                                                                           gaTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGa                                                                 >  2:1415010/1‑149 (MQ=255)
                                                                           gaTAGCCCGCTAATCAGTCCCAAAATCAACCCCAACGATCCGCCAATGGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         <  1:1394830/93‑1 (MQ=255)
                                                                            aTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGaa                                                                <  3:164339/149‑1 (MQ=255)
                                                                            aTAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGaa                                                                <  3:1389380/149‑1 (MQ=255)
                                                                                cccGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGga                                                                                                     >  4:670244/1‑108 (MQ=255)
                                                                                cccGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGga                                                                                                     <  3:670244/108‑1 (MQ=255)
                                                                                 ccGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCaa                                                           >  3:694407/1‑149 (MQ=255)
                                                                                   gCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCaaaa                                                         >  2:671610/1‑149 (MQ=255)
                                                                                     tAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATa                                                       <  3:994332/149‑1 (MQ=255)
                                                                                     tAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATa                                                       <  4:694407/149‑1 (MQ=255)
                                                                                       aTCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                       >  4:1118211/1‑131 (MQ=255)
                                                                                       aTCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                       <  3:1118211/131‑1 (MQ=255)
                                                                                       aTCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATAcg                                                     >  4:296157/1‑149 (MQ=255)
                                                                                        tCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCTCCTGGCGGGCGCTGAtt                                                                                                                                     <  3:372817/68‑2 (MQ=25)
                                                                                               cAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAca                                             >  4:287817/1‑149 (MQ=255)
                                                                                               cAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAca                                             >  2:110676/1‑149 (MQ=255)
                                                                                                aaaaTCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAcag                                            >  2:433789/1‑149 (MQ=255)
                                                                                                    tCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCCGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTcc                                                                                             >  2:253571/1‑96 (MQ=255)
                                                                                                    tCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTcc                                                                                             <  1:253571/96‑1 (MQ=255)
                                                                                                         cccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         <  3:1004351/63‑1 (MQ=255)
                                                                                                         cccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         >  1:1340107/1‑63 (MQ=255)
                                                                                                         cccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         <  2:1340107/63‑1 (MQ=255)
                                                                                                         cccAGCGATCCCCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctgc                                                                                                                         >  4:1004351/1‑63 (MQ=255)
                                                                                                          ccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                       <  2:809290/112‑1 (MQ=255)
                                                                                                          ccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                       >  1:809290/1‑112 (MQ=255)
                                                                                                          ccAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAAcc                                  >  4:1162918/1‑149 (MQ=255)
                                                                                                           cAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGTcaca                                                                                                                     <  4:846783/65‑1 (MQ=255)
                                                                                                           cAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGTcaca                                                                                                                     >  3:846783/1‑65 (MQ=255)
                                                                                                           cAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGgaga                                                                                                   >  3:417699/1‑83 (MQ=255)
                                                                                                           cAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGgaga                                                                                                   <  4:417699/83‑1 (MQ=255)
                                                                                                             gCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCgag                               <  3:174295/149‑1 (MQ=255)
                                                                                                               gATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                       <  2:1283537/107‑1 (MQ=255)
                                                                                                               gATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTc                                                                       >  1:1283537/1‑107 (MQ=255)
                                                                                                                aTCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCgaggag                            >  4:1027070/1‑149 (MQ=255)
                                                                                                                 tCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTcc                                                                      >  1:1451971/1‑106 (MQ=255)
                                                                                                                 tCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTcc                                                                      <  2:1451971/106‑1 (MQ=255)
                                                                                                                  ccGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGc                          <  3:1027070/149‑1 (MQ=255)
                                                                                                                          tGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGcc                  <  1:110676/149‑1 (MQ=255)
                                                                                                                           gAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCgagga                             <  3:230079/137‑1 (MQ=255)
                                                                                                                           gAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCgagga                             >  4:230079/1‑137 (MQ=255)
                                                                                                                           gAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCca                 <  1:671610/149‑1 (MQ=255)
                                                                                                                            aaaaGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAca                                             <  1:481789/120‑1 (MQ=255)
                                                                                                                            aaaaGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGAca                                             >  2:481789/1‑120 (MQ=255)
                                                                                                                            aaaaGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCgag                               <  1:1163695/134‑1 (MQ=255)
                                                                                                                            aaaaGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGAGAAGGCAAAATACGCGTCGACAGCCAGCCAACCgag                               >  2:1163695/1‑134 (MQ=255)
                                                                                                                              aaGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCacac              >  1:703062/1‑149 (MQ=255)
                                                                                                                              aaGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCacac              >  2:1418605/1‑149 (MQ=255)
                                                                                                                                gCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGtt                     >  1:106088/1‑140 (MQ=255)
                                                                                                                                gCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGtt                     <  2:106088/140‑1 (MQ=255)
                                                                                                                                gCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCg            >  2:601557/1‑149 (MQ=255)
                                                                                                                                   aaTCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcac                                                                                >  4:1149532/1‑78 (MQ=255)
                                                                                                                                   aaTCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTcac                                                                                <  3:1149532/78‑1 (MQ=255)
                                                                                                                                    aTCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCAc        >  2:1003236/1‑149 (MQ=255)
                                                                                                                                     tCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACg       >  2:1067859/1‑149 (MQ=255)
                                                                                                                                     tCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACg       >  1:1030447/1‑149 (MQ=255)
                                                                                                                                      cAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGa      <  1:601557/149‑1 (MQ=255)
                                                                                                                                         cgcccgccAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGc   >  3:190813/1‑149 (MQ=255)
                                                                                                                                          gcccgccAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGcc                  >  1:1003013/1‑133 (MQ=255)
                                                                                                                                          gcccgccAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGcc                  <  2:1003013/133‑1 (MQ=255)
                                                                                                                                          gcccgccAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGcc  <  1:433789/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
AGCGGGATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCCACAGTTCGCCGCTGGCGGAGAGCGTCCAGAACGCCGTCACCACTCCTTCCGGTGAAGGCAAAATACGCGTCGACAGCCAGCCAACCGAGGAGGCCAGTTGCCACACCGCCACGATGCC  >  NC_000913/994489‑994775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: