Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,064,161 G→A 5.5% L42L (CTG→CTA) ‡ yccE → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,064,1610GA 5.5% 365.8 / 7.6 109L42L (CTG→CTA) ‡yccEuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (52/51);  new base A (3/3);  total (55/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 109 total aligned reads displayed.

ATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTA  >  NC_000913/1064034‑1064306
                                                                                                                               |                                                                                                                                                 
atatGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCgg                                                                                                                                    <  4:1361431/143‑1 (MQ=255)
atatGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCgg                                                                                                                                    >  3:1361431/1‑143 (MQ=255)
         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGt                                                                                                                                   <  2:242293/135‑1 (MQ=255)
         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGt                                                                                                                                   >  1:242293/1‑135 (MQ=255)
                  aTGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGttt                                                                                                                                           >  1:1009061/1‑118 (MQ=255)
                  aTGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGttt                                                                                                                                           <  2:1009061/118‑1 (MQ=255)
                     gAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagta                                                                                                         >  3:119229/1‑149 (MQ=255)
                           gTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGaa                                                                                                   <  3:935310/149‑1 (MQ=255)
                                      aataatTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGa                                                                                                                  >  4:464732/1‑123 (MQ=255)
                                        taatTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCAt                                                                                      >  3:804011/1‑149 (MQ=255)
                                          attattTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                 <  2:810890/136‑1 (MQ=255)
                                          attattTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                 >  1:810890/1‑136 (MQ=255)
                                           ttattTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGtt                                                                                                                                            >  3:911827/1‑92 (MQ=255)
                                           ttattTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGtt                                                                                                                                            <  4:911827/92‑1 (MQ=255)
                                           ttattTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTc                                                                                   <  4:119229/149‑1 (MQ=255)
                                              tttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       <  1:1024611/110‑1 (MQ=255)
                                              tttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       >  2:1024611/1‑110 (MQ=255)
                                              tttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                >  4:1073577/1‑149 (MQ=255)
                                               ttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGa                                                                               <  4:804011/149‑1 (MQ=255)
                                                tGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGaa                                                                              >  3:557729/1‑149 (MQ=255)
                                                 gAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       >  3:362887/1‑107 (MQ=255)
                                                 gAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       <  4:362887/107‑1 (MQ=255)
                                                         ccTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCa                                                                     <  4:557729/149‑1 (MQ=255)
                                                          cTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGa                                                                         <  3:1263478/144‑1 (MQ=255)
                                                          cTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGa                                                                         >  4:1263478/1‑144 (MQ=255)
                                                               aCGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACaa                                                                                                                            <  2:1363298/88‑1 (MQ=255)
                                                               aCGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACaa                                                                                                                            >  1:1363298/1‑88 (MQ=255)
                                                                      aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtgt                                                                                                                                             <  3:1470281/64‑1 (MQ=255)
                                                                      aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgt                                                                                                                                                 <  4:318136/60‑1 (MQ=255)
                                                                      aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgt                                                                                                                                                 >  3:318136/1‑60 (MQ=255)
                                                                       aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                 <  4:619978/107‑1 (MQ=255)
                                                                       aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                 >  3:1190134/1‑107 (MQ=255)
                                                                       aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                 >  3:619978/1‑107 (MQ=255)
                                                                       aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                 <  4:1190134/107‑1 (MQ=255)
                                                                       aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaaa                                                            >  4:1438970/1‑144 (MQ=255)
                                                                       aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaaa                                                            <  3:1438970/144‑1 (MQ=255)
                                                                        aaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       >  1:757909/1‑84 (MQ=255)
                                                                        aaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       <  2:757909/84‑1 (MQ=255)
                                                                          aaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                    <  3:1073577/149‑1 (MQ=255)
                                                                                tACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTtata                                                                                                                               <  2:918675/68‑1 (MQ=255)
                                                                                tACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGAGTGTTTTATGCGGTtata                                                                                                                               >  1:918675/1‑68 (MQ=255)
                                                                                         aaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGa                                                                                              >  1:1358827/1‑92 (MQ=255)
                                                                                         aaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGa                                                                                              <  2:1358827/92‑1 (MQ=255)
                                                                                           tCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagtaa                                                                                                        <  3:1241965/80‑1 (MQ=255)
                                                                                           tCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagtaa                                                                                                        >  4:1241965/1‑80 (MQ=255)
                                                                                             aaaCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTa                                                                  <  1:868013/116‑1 (MQ=255)
                                                                                             aaaCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTa                                                                  >  2:868013/1‑116 (MQ=255)
                                                                                             aaaCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataat                                 >  3:357025/1‑149 (MQ=255)
                                                                                                 atatTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATc                             <  1:950013/149‑1 (MQ=255)
                                                                                                 atatTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATc                             >  2:950013/1‑149 (MQ=255)
                                                                                                   atTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGaa                                                         <  1:1366524/119‑1 (MQ=255)
                                                                                                   atTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGaa                                                         >  2:1366524/1‑119 (MQ=255)
                                                                                                    ttCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAtt                                                       <  3:494868/120‑1 (MQ=255)
                                                                                                    ttCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAtt                                                       >  4:494868/1‑120 (MQ=255)
                                                                                                       aaTCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCat                       >  3:735765/1‑149 (MQ=255)
                                                                                                       aaTCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCat                       >  1:151166/1‑149 (MQ=255)
                                                                                                       aaTCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCat                       >  1:230982/1‑149 (MQ=255)
                                                                                                         tCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGa                                         <  1:1229035/129‑1 (MQ=255)
                                                                                                         tCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGa                                         >  2:1229035/1‑129 (MQ=255)
                                                                                                          cACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAAt                                                                                                                           >  3:1152119/1‑46 (MQ=255)
                                                                                                           aCGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCa                                                                                                                    <  4:1218151/52‑1 (MQ=255)
                                                                                                              cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCAttatttat                <  4:735765/149‑1 (MQ=255)
                                                                                                              cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCAttatttat                <  2:230982/149‑1 (MQ=255)
                                                                                                               tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       >  4:844448/1‑45 (MQ=255)
                                                                                                               tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                       <  3:844448/45‑1 (MQ=255)
                                                                                                               tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                    <  1:503775/112‑1 (MQ=255)
                                                                                                               tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                    >  2:503775/1‑112 (MQ=255)
                                                                                                                ttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATga              <  4:357025/149‑1 (MQ=255)
                                                                                                                 gcataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCCGGAATGTGAATCACTAGaaaaa                                                            >  3:1036034/5‑102 (MQ=255)
                                                                                                                 gcataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCCGGAATGTGAATCACTAGaaaaa                                                            <  4:1036034/98‑1 (MQ=255)
                                                                                                                   ataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAg                                                                 >  2:838426/3‑95 (MQ=255)
                                                                                                                   ataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAg                                                                 <  1:838426/93‑1 (MQ=255)
                                                                                                                    gAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTAt                          >  2:243197/1‑133 (MQ=255)
                                                                                                                     aaaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGATGTAAAGTAAATGAagag                                                                                                >  2:17320/1‑62 (MQ=255)
                                                                                                                     aaaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGATGTAAAGTAAATGAagag                                                                                                <  1:17320/62‑1 (MQ=255)
                                                                                                                     aaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAg                                                   >  4:1179766/1‑107 (MQ=255)
                                                                                                                     aaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAg                                                   <  3:1179766/107‑1 (MQ=255)
                                                                                                                      aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAt                                                                       >  4:1298033/1‑86 (MQ=255)
                                                                                                                      aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAt                                                                       <  3:1298033/86‑1 (MQ=255)
                                                                                                                      aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaa                                                             <  2:529032/96‑1 (MQ=255)
                                                                                                                      aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaa                                                             >  1:529032/1‑96 (MQ=255)
                                                                                                                      aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                    <  2:496168/105‑1 (MQ=255)
                                                                                                                      aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                    >  1:496168/1‑105 (MQ=255)
                                                                                                                       aaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAt                                                                       >  3:1442575/1‑85 (MQ=255)
                                                                                                                       aaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAt                                                                       <  4:1442575/85‑1 (MQ=255)
                                                                                                                       aaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCa                                                                     <  1:190908/87‑1 (MQ=255)
                                                                                                                        acacacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGa                                                                                              <  4:1432666/61‑1 (MQ=255)
                                                                                                                        acacacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGa                                                                                              >  3:1432666/1‑61 (MQ=255)
                                                                                                                          acacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGaa                                                         <  2:250941/96‑1 (MQ=255)
                                                                                                                          acacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGaa                                                         >  1:250941/1‑96 (MQ=255)
                                                                                                                          acacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGc                                            >  1:815042/1‑109 (MQ=255)
                                                                                                                          acacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTAt                          <  2:906909/127‑1 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAgg                                                                                               <  2:1248109/56‑1 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAgg                                                                                               >  1:1248109/1‑56 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                          >  2:547465/1‑109 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                          <  1:547465/109‑1 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCg    <  2:522597/147‑1 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCg    >  1:522597/1‑147 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTa  <  2:151166/149‑1 (MQ=255)
                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTa  >  4:1090749/1‑149 (MQ=255)
                                                                                                                               |                                                                                                                                                 
ATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTA  >  NC_000913/1064034‑1064306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: