Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,212,482 G→T 7.4% intergenic (‑479/+221) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,4820GT 7.4% 187.9 / 5.0 54intergenic (‑479/+221)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base T (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAG  >  NC_000913/1212334‑1212629
                                                                                                                                                    |                                                                                                                                                   
atGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACgg                                                                                                                                                     <  2:380184/149‑1 (MQ=255)
atGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACgg                                                                                                                                                     >  1:380184/1‑149 (MQ=255)
                      aaTAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGttt                                                                                                                                            <  3:1015290/136‑1 (MQ=255)
                      aaTAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGttt                                                                                                                                            >  4:1015290/1‑136 (MQ=255)
                                                   gAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgtt                                                                                                                                                <  3:1373675/103‑1 (MQ=255)
                                                   gAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgtt                                                                                                                                                >  4:1373675/1‑103 (MQ=255)
                                                    aaTGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                           <  1:138380/107‑1 (MQ=255)
                                                    aaTGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                           >  2:138380/1‑107 (MQ=255)
                                                        aTTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTg                                                                                             >  4:195126/1‑149 (MQ=255)
                                                             aCCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGttttt                                                                                                                                          >  3:24421/1‑99 (MQ=255)
                                                             aCCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGttttt                                                                                                                                          <  4:24421/99‑1 (MQ=255)
                                                                      tGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTta                                                                                                                                         >  2:989074/1‑91 (MQ=255)
                                                                      tGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTta                                                                                                                                         <  1:989074/91‑1 (MQ=255)
                                                                          tAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaa                                                                                 <  4:919577/143‑1 (MQ=255)
                                                                          tAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaa                                                                                 >  3:919577/1‑143 (MQ=255)
                                                                                       aCCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       <  2:1408110/92‑1 (MQ=255)
                                                                                       aCCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       >  1:1408110/1‑92 (MQ=255)
                                                                                               atgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGt                                                      <  3:195126/149‑1 (MQ=255)
                                                                                                  aCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATc                                                                                                         <  3:761643/95‑1 (MQ=255)
                                                                                                  aCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATc                                                                                                         >  4:761643/1‑95 (MQ=255)
                                                                                                   cTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACAttt                                                                                    >  4:635021/1‑115 (MQ=255)
                                                                                                   cTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACAttt                                                                                    <  3:635021/115‑1 (MQ=255)
                                                                                                    tAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGatta                                                 >  1:91769/1‑149 (MQ=255)
                                                                                                       aCCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGAttattt                                              >  4:174736/1‑149 (MQ=255)
                                                                                                         cAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCaaaa                                                                                                     >  2:119168/1‑92 (MQ=255)
                                                                                                         cAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCaaaa                                                                                                     <  1:119168/92‑1 (MQ=255)
                                                                                                         cAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGa                                                                     <  3:666060/124‑1 (MQ=255)
                                                                                                         cAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGa                                                                     >  4:666060/1‑124 (MQ=255)
                                                                                                          aaaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATg                                           <  3:174736/149‑1 (MQ=255)
                                                                                                           aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTa                                                                                                           <  3:1292170/84‑1 (MQ=255)
                                                                                                           aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTa                                                                                                           >  4:1292170/1‑84 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGAt                                                                                           >  1:138271/1‑92 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGAt                                                                                           <  2:138271/92‑1 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACAtt                                                                                     <  4:895419/98‑1 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACAtt                                                                                     >  3:895419/1‑98 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATg                                           <  4:797852/140‑1 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATg                                           >  3:797852/1‑140 (MQ=255)
                                                                                                                        ttATGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       <  1:1277628/59‑1 (MQ=255)
                                                                                                                        ttAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       >  2:1277628/1‑59 (MQ=255)
                                                                                                                            gTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACa                         <  2:91769/149‑1 (MQ=255)
                                                                                                                               ttGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                      >  4:425625/1‑149 (MQ=255)
                                                                                                                               ttGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                      >  2:648198/1‑149 (MQ=255)
                                                                                                                                 gAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaaaaa                             >  2:333170/1‑140 (MQ=255)
                                                                                                                                 gAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaaaaa                             <  1:333170/140‑1 (MQ=255)
                                                                                                                                   aaCGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATg                                           >  1:167935/1‑124 (MQ=255)
                                                                                                                                   aaCGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATg                                           <  2:167935/124‑1 (MQ=255)
                                                                                                                                     cGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAatc                <  1:648198/149‑1 (MQ=255)
                                                                                                                                     cGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAatc                <  3:425625/149‑1 (MQ=255)
                                                                                                                                            gtgGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       <  4:33610/39‑1 (MQ=255)
                                                                                                                                            gtgGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       >  3:33610/1‑39 (MQ=255)
                                                                                                                                            gtgGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaaa                                                                                >  2:259200/1‑78 (MQ=255)
                                                                                                                                            gtgGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaaa                                                                                <  1:259200/78‑1 (MQ=255)
                                                                                                                                            gccacACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATa         <  2:701456/145‑1 (MQ=255)
                                                                                                                                            gccacACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATa         >  4:32855/5‑149 (MQ=255)
                                                                                                                                            gccacACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGt                                                           >  4:54971/5‑99 (MQ=255)
                                                                                                                                            gccacACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGt                                                           <  3:54971/95‑1 (MQ=255)
                                                                                                                                                   ggCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAg  >  4:496770/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAG  >  NC_000913/1212334‑1212629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: