Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,231,963 | T→A | 6.3% | Y260N (TAT→AAT) | umuC → | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,231,963 | 0 | T | A | 6.3% | 407.4 / 13.3 | 111 | Y260N (TAT→AAT) | umuC | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
Reads supporting (aligned to +/- strand): ref base T (52/52); new base A (4/3); total (56/55) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 |
Only 100 of 111 total aligned reads displayed.
TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGC > NC_000913/1231817‑1232106 | tCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGTAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTAt > 4:1282434/1‑149 (MQ=255) tCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTAt > 4:581465/1‑149 (MQ=255) ccGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAAt > 4:769573/1‑149 (MQ=255) cGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATcc > 4:1467089/1‑149 (MQ=255) aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGAt < 2:258644/149‑1 (MQ=255) aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGAt < 4:303710/149‑1 (MQ=255) ccGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGc < 2:1397025/149‑1 (MQ=255) gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCgg > 3:637538/1‑149 (MQ=255) tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCGTC‑ACGGATTATCCGTCGATGCGGc > 1:1172470/1‑149 (MQ=255) tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGT‑GATGCgtt < 3:769573/149‑3 (MQ=255) tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGc > 1:42777/1‑149 (MQ=255) aaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAg < 2:1172470/149‑1 (MQ=255) aaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAg > 2:481114/1‑149 (MQ=255) tttAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCAt < 4:637538/149‑1 (MQ=255) gTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAg > 2:51554/1‑149 (MQ=255) tGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTAc < 2:42777/149‑1 (MQ=255) gCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACg > 1:1261016/1‑149 (MQ=255) gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc < 2:1261016/149‑1 (MQ=255) gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc > 1:1073767/1‑149 (MQ=255) gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc > 2:774918/1‑149 (MQ=255) aaCGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTa > 3:657648/1‑134 (MQ=255) aaCGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTa < 4:657648/134‑1 (MQ=255) gAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaa > 1:770153/1‑149 (MQ=255) aaCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa < 3:1467089/149‑1 (MQ=255) cTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACt > 1:1422095/1‑149 (MQ=255) cggcgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa < 1:774918/149‑1 (MQ=255) ggcgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAg > 3:133966/1‑149 (MQ=255) cgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCg > 4:651783/1‑149 (MQ=255) aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa > 1:318889/1‑143 (MQ=255) aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa < 2:318889/143‑1 (MQ=255) aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGc > 2:809283/1‑149 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATc > 3:83856/1‑84 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATc < 4:83856/84‑1 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGa > 2:973864/1‑145 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGa < 1:973864/145‑1 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAt < 2:1422095/149‑1 (MQ=255) cTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATc > 1:114426/1‑149 (MQ=255) tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAAc > 4:281291/1‑133 (MQ=255) tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAAc < 3:281291/133‑1 (MQ=255) tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCa > 2:14472/1‑149 (MQ=255) gTTTACAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCaa < 4:1235819/149‑1 (MQ=255) aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt < 1:51554/149‑1 (MQ=255) aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt > 1:1434548/1‑149 (MQ=255) aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt > 1:694086/1‑149 (MQ=255) aCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc < 1:809283/149‑1 (MQ=255) cTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCg > 1:157542/1‑149 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTa < 1:863705/94‑1 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTa > 2:863705/1‑94 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt > 1:347284/1‑149 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt > 4:804553/1‑149 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt < 2:1073767/149‑1 (MQ=255) gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt > 1:647770/1‑147 (MQ=255) gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt < 2:647770/147‑1 (MQ=255) gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAt < 2:157542/149‑1 (MQ=255) gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAt < 2:1434548/149‑1 (MQ=255) gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGc < 4:237038/124‑1 (MQ=255) gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGc > 3:237038/1‑124 (MQ=255) tttGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAtctc > 3:66036/1‑149 (MQ=255) gCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCAc < 4:66036/149‑1 (MQ=255) gCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTAGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGCTTTATCTCCAc < 3:1282434/149‑1 (MQ=255) gacgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTtta < 3:804553/149‑1 (MQ=255) cgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttatt < 2:770153/149‑1 (MQ=255) gaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCa > 4:1152362/1‑71 (MQ=255) gaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCa < 3:1152362/71‑1 (MQ=255) aGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAg < 3:651783/149‑1 (MQ=255) aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATc < 1:1156580/48‑1 (MQ=255) aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATc > 2:1156580/1‑48 (MQ=255) aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGgcggcg > 1:1454627/1‑92 (MQ=255) aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGgcggcg < 2:1454627/92‑1 (MQ=255) ggAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt > 1:861058/1‑100 (MQ=255) ggAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt < 2:861058/100‑1 (MQ=255) aaaTTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa < 2:114426/149‑1 (MQ=255) aTCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt > 1:308505/1‑149 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt < 1:1407448/71‑1 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt > 2:662676/1‑71 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt < 1:662676/71‑1 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAttt > 3:442585/1‑149 (MQ=255) cTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATAGTCGGTTTATCTCCACGTTTATTAAGACGTCACCGTTTg > 4:1235342/1‑149 (MQ=255) ccGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATAtt > 3:647909/1‑104 (MQ=255) ccGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATAtt < 4:647909/104‑1 (MQ=255) cGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa > 2:635927/1‑128 (MQ=255) cGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa < 1:635927/128‑1 (MQ=255) tCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGa < 1:14472/149‑1 (MQ=255) cGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa < 2:1439497/149‑1 (MQ=255) gTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGGTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc < 2:347284/149‑1 (MQ=255) aaCGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCg < 3:117049/94‑1 (MQ=255) catcgaCGGATAATCCGT‑GATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa > 1:326036/6‑111 (MQ=255) catcgaCGGATAATCCGT‑GATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa < 2:326036/106‑1 (MQ=255) atcgaCGGATAATCCGT‑GATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa < 2:1319549/113‑1 (MQ=255) atcgaCGGATAATCCGT‑GATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa > 1:1319549/5‑117 (MQ=255) tC‑ACGGATTATCTGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc < 1:14532/135‑1 (MQ=255) tC‑ACGGATTATCTGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc > 2:14532/1‑135 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGc > 4:374254/1‑75 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGc < 3:374254/75‑1 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa < 4:208392/109‑1 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa > 3:208392/1‑109 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATa < 3:1235342/149‑1 (MQ=255) aCGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCa > 3:396910/1‑117 (MQ=255) aCGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAg > 1:1263603/1‑149 (MQ=255) aCGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAg > 2:223448/1‑149 (MQ=255) | TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGC > NC_000913/1231817‑1232106 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |