Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,231,963 T→A 6.3% Y260N (TAT→AAT)  umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,231,9630TA 6.3% 407.4 / 13.3 111Y260N (TAT→AAT) umuCtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity
Reads supporting (aligned to +/- strand):  ref base T (52/52);  new base A (4/3);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

Only 100 of 111 total aligned reads displayed.

TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGC  >  NC_000913/1231817‑1232106
                                                                                                                                                   |                                                                                                                                               
tCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGTAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTAt                                                                                                                                               >  4:1282434/1‑149 (MQ=255)
tCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTAt                                                                                                                                               >  4:581465/1‑149 (MQ=255)
 ccGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAAt                                                                                                                                               >  4:769573/1‑149 (MQ=255)
  cGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATcc                                                                                                                                             >  4:1467089/1‑149 (MQ=255)
        aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGAt                                                                                                                                       <  2:258644/149‑1 (MQ=255)
        aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGAt                                                                                                                                       <  4:303710/149‑1 (MQ=255)
          ccGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGc                                                                                                                                     <  2:1397025/149‑1 (MQ=255)
            gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCgg                                                                                                                                   >  3:637538/1‑149 (MQ=255)
             tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCGTCACGGATTATCCGTCGATGCGGc                                                                                                                                  >  1:1172470/1‑149 (MQ=255)
             tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGTGATGCgtt                                                                                                                                  <  3:769573/149‑3 (MQ=255)
             tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGc                                                                                                                                  >  1:42777/1‑149 (MQ=255)
               aaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAg                                                                                                                                <  2:1172470/149‑1 (MQ=255)
               aaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAg                                                                                                                                >  2:481114/1‑149 (MQ=255)
                    tttAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCAt                                                                                                                           <  4:637538/149‑1 (MQ=255)
                          gTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAg                                                                                                                     >  2:51554/1‑149 (MQ=255)
                              tGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTAc                                                                                                                 <  2:42777/149‑1 (MQ=255)
                               gCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACg                                                                                                                >  1:1261016/1‑149 (MQ=255)
                                   gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc                                                                                                            <  2:1261016/149‑1 (MQ=255)
                                   gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc                                                                                                            >  1:1073767/1‑149 (MQ=255)
                                   gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc                                                                                                            >  2:774918/1‑149 (MQ=255)
                                        aaCGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTa                                                                                                                      >  3:657648/1‑134 (MQ=255)
                                        aaCGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTa                                                                                                                      <  4:657648/134‑1 (MQ=255)
                                                  gAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaa                                                                                             >  1:770153/1‑149 (MQ=255)
                                                   aaCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa                                                                                            <  3:1467089/149‑1 (MQ=255)
                                                     cTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACt                                                                                          >  1:1422095/1‑149 (MQ=255)
                                                          cggcgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa                                                                                     <  1:774918/149‑1 (MQ=255)
                                                           ggcgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAg                                                                                    >  3:133966/1‑149 (MQ=255)
                                                             cgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCg                                                                                  >  4:651783/1‑149 (MQ=255)
                                                                aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa                                                                                     >  1:318889/1‑143 (MQ=255)
                                                                aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa                                                                                     <  2:318889/143‑1 (MQ=255)
                                                                aCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGc                                                                               >  2:809283/1‑149 (MQ=255)
                                                                  ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATc                                                                                                                                              >  3:83856/1‑84 (MQ=255)
                                                                  ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATc                                                                                                                                              <  4:83856/84‑1 (MQ=255)
                                                                  ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGa                                                                                 >  2:973864/1‑145 (MQ=255)
                                                                  ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGa                                                                                 <  1:973864/145‑1 (MQ=255)
                                                                  ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAt                                                                             <  2:1422095/149‑1 (MQ=255)
                                                                   cTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATc                                                                            >  1:114426/1‑149 (MQ=255)
                                                                    tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAAc                                                                                           >  4:281291/1‑133 (MQ=255)
                                                                    tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAAc                                                                                           <  3:281291/133‑1 (MQ=255)
                                                                    tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCa                                                                           >  2:14472/1‑149 (MQ=255)
                                                                     gTTTACAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCaa                                                                          <  4:1235819/149‑1 (MQ=255)
                                                                           aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt                                                                    <  1:51554/149‑1 (MQ=255)
                                                                           aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt                                                                    >  1:1434548/1‑149 (MQ=255)
                                                                           aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt                                                                    >  1:694086/1‑149 (MQ=255)
                                                                            aCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc                                                                   <  1:809283/149‑1 (MQ=255)
                                                                             cTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCg                                                                  >  1:157542/1‑149 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTa                                                                                                                      <  1:863705/94‑1 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTa                                                                                                                      >  2:863705/1‑94 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt                                                               >  1:347284/1‑149 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt                                                               >  4:804553/1‑149 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt                                                               <  2:1073767/149‑1 (MQ=255)
                                                                                   gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                              >  1:647770/1‑147 (MQ=255)
                                                                                   gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                              <  2:647770/147‑1 (MQ=255)
                                                                                   gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAt                                                            <  2:157542/149‑1 (MQ=255)
                                                                                   gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAt                                                            <  2:1434548/149‑1 (MQ=255)
                                                                                     gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGc                                                                                   <  4:237038/124‑1 (MQ=255)
                                                                                     gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGc                                                                                   >  3:237038/1‑124 (MQ=255)
                                                                                      tttGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAtctc                                                         >  3:66036/1‑149 (MQ=255)
                                                                                         gCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCAc                                                      <  4:66036/149‑1 (MQ=255)
                                                                                         gCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTAGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGCTTTATCTCCAc                                                      <  3:1282434/149‑1 (MQ=255)
                                                                                              gacgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTtta                                                 <  3:804553/149‑1 (MQ=255)
                                                                                                cgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttatt                                               <  2:770153/149‑1 (MQ=255)
                                                                                                 gaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCa                                                                                                                            >  4:1152362/1‑71 (MQ=255)
                                                                                                 gaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCa                                                                                                                            <  3:1152362/71‑1 (MQ=255)
                                                                                                   aGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAg                                            <  3:651783/149‑1 (MQ=255)
                                                                                                      aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATc                                                                                                                                              <  1:1156580/48‑1 (MQ=255)
                                                                                                      aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATc                                                                                                                                              >  2:1156580/1‑48 (MQ=255)
                                                                                                      aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGgcggcg                                                                                                  >  1:1454627/1‑92 (MQ=255)
                                                                                                      aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGgcggcg                                                                                                  <  2:1454627/92‑1 (MQ=255)
                                                                                                       ggAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt                                                                                         >  1:861058/1‑100 (MQ=255)
                                                                                                       ggAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACtt                                                                                         <  2:861058/100‑1 (MQ=255)
                                                                                                         aaaTTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa                                      <  2:114426/149‑1 (MQ=255)
                                                                                                              aTCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAtt                                 >  1:308505/1‑149 (MQ=255)
                                                                                                               tCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt                                                                                                              <  1:1407448/71‑1 (MQ=255)
                                                                                                               tCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt                                                                                                              >  2:662676/1‑71 (MQ=255)
                                                                                                               tCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt                                                                                                              <  1:662676/71‑1 (MQ=255)
                                                                                                               tCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCAttt                                >  3:442585/1‑149 (MQ=255)
                                                                                                                cTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATAGTCGGTTTATCTCCACGTTTATTAAGACGTCACCGTTTg                               >  4:1235342/1‑149 (MQ=255)
                                                                                                                      ccGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATAtt                                                                      >  3:647909/1‑104 (MQ=255)
                                                                                                                      ccGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATAtt                                                                      <  4:647909/104‑1 (MQ=255)
                                                                                                                       cGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                             >  2:635927/1‑128 (MQ=255)
                                                                                                                       cGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                             <  1:635927/128‑1 (MQ=255)
                                                                                                                          tCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGa                     <  1:14472/149‑1 (MQ=255)
                                                                                                                           cGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa                    <  2:1439497/149‑1 (MQ=255)
                                                                                                                            gTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGGTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc                   <  2:347284/149‑1 (MQ=255)
                                                                                                                                    aaCGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCg                                                                  <  3:117049/94‑1 (MQ=255)
                                                                                                                                        catcgaCGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                             >  1:326036/6‑111 (MQ=255)
                                                                                                                                        catcgaCGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                             <  2:326036/106‑1 (MQ=255)
                                                                                                                                         atcgaCGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa                                      <  2:1319549/113‑1 (MQ=255)
                                                                                                                                         atcgaCGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa                                      >  1:1319549/5‑117 (MQ=255)
                                                                                                                                          tCACGGATTATCTGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc                   <  1:14532/135‑1 (MQ=255)
                                                                                                                                          tCACGGATTATCTGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc                   >  2:14532/1‑135 (MQ=255)
                                                                                                                                          tCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGc                                                                               >  4:374254/1‑75 (MQ=255)
                                                                                                                                          tCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGc                                                                               <  3:374254/75‑1 (MQ=255)
                                                                                                                                          tCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                             <  4:208392/109‑1 (MQ=255)
                                                                                                                                          tCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                             >  3:208392/1‑109 (MQ=255)
                                                                                                                                           cACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATa    <  3:1235342/149‑1 (MQ=255)
                                                                                                                                             aCGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCa                                   >  3:396910/1‑117 (MQ=255)
                                                                                                                                             aCGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAg   >  1:1263603/1‑149 (MQ=255)
                                                                                                                                             aCGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAg   >  2:223448/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGC  >  NC_000913/1231817‑1232106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: