Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,304,474 T→A 5.8% I214N (ATT→AAT)  oppC → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,304,4740TA 5.8% 611.9 / 21.2 172I214N (ATT→AAT) oppColigopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (79/83);  new base A (6/4);  total (85/87)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

Only 100 of 180 total aligned reads displayed.

TGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATGGCG  >  NC_000913/1304327‑1304617
                                                                                                                                                   |                                                                                                                                               
tgctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACAtt                                                                                                                                                >  3:696483/1‑149 (MQ=255)
tgctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTAACGACATCGGGCATTGTTATTCGCCACAtt                                                                                                                                                >  3:1428232/1‑149 (MQ=255)
  ctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              >  1:319725/1‑149 (MQ=255)
  ctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              <  2:261350/149‑1 (MQ=255)
  ctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              <  2:319725/149‑1 (MQ=255)
  ctgATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              >  2:423327/1‑149 (MQ=255)
         tCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAAt                                                                                                                                       <  4:719714/149‑1 (MQ=255)
             ggCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTAc                                                                                                                                   >  3:207078/1‑149 (MQ=255)
                gATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTAc                                                                                                                                   <  3:975122/146‑1 (MQ=255)
                gATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTAc                                                                                                                                   >  4:975122/1‑146 (MQ=255)
                gATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCg                                                                                                                                >  4:245495/1‑149 (MQ=255)
                     gCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGtgtg                                                                                                                           >  4:141582/1‑149 (MQ=255)
                     gCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGtgtg                                                                                                                           >  4:461143/1‑149 (MQ=255)
                      cATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              >  3:965302/1‑129 (MQ=255)
                      cATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtgg                                                                                                                          <  2:570356/149‑1 (MQ=255)
                        tGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtggtg                                                                                                                        >  4:58888/1‑149 (MQ=255)
                          gTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACAt                                                                                                                                                 <  4:678220/122‑1 (MQ=255)
                          gTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                            >  4:1399426/1‑143 (MQ=255)
                          gTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                            <  3:1399426/143‑1 (MQ=255)
                          gTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtggtggt                                                                                                                      <  3:141582/149‑1 (MQ=255)
                               ctggctggATGTGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCt                                                                                                                 >  1:1108607/1‑149 (MQ=255)
                               ctggctggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgt                                                                                                                              <  3:64969/136‑1 (MQ=255)
                                tggctggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTa                                                                                                                >  2:71076/1‑149 (MQ=255)
                                    tggATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTCCGCa                                                                                                            >  4:1016375/1‑149 (MQ=255)
                                       atatGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCa                                                                                                         >  1:666487/1‑149 (MQ=255)
                                            gCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCAt                                                                                                           <  2:1040952/142‑1 (MQ=255)
                                            gCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCAt                                                                                                           >  1:1040952/1‑142 (MQ=255)
                                              tCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCACAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACAt                                                                                                                                                 <  3:1235721/102‑1 (MQ=255)
                                              tCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACAt                                                                                                                                                 >  4:1235721/1‑102 (MQ=255)
                                               cGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGt                                                                                                 <  4:1318331/149‑1 (MQ=255)
                                               cGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGt                                                                                                 >  3:154843/1‑149 (MQ=255)
                                                   ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              <  4:1124860/100‑1 (MQ=255)
                                                   ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATtgt                                                                                                                                              >  3:1124860/1‑100 (MQ=255)
                                                   ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAAt                                                                                                                                       <  1:1285780/107‑1 (MQ=255)
                                                   ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAAt                                                                                                                                       >  2:1285780/1‑107 (MQ=255)
                                                   ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGccc                                                                                             >  1:419526/1‑149 (MQ=255)
                                                   ttGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGccc                                                                                             >  3:638283/1‑149 (MQ=255)
                                                     gtgCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAg                                                                                           <  3:58888/149‑1 (MQ=255)
                                                       gCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCa                                                                                         <  2:1108607/149‑1 (MQ=255)
                                                       gCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCa                                                                                         <  2:666487/149‑1 (MQ=255)
                                                            ggCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATc                                                                                    <  3:1016375/149‑1 (MQ=255)
                                                               aaaCCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCctc                                                                                 >  3:36146/1‑149 (MQ=255)
                                                                  cccTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGa                                                                                      <  3:441047/141‑1 (MQ=255)
                                                                   ccTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTg                                                                             >  2:136788/1‑149 (MQ=255)
                                                                      gAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATc                                                                                    <  4:944061/139‑1 (MQ=255)
                                                                         tCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCtt                                                                       <  4:154843/149‑1 (MQ=255)
                                                                           tGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTAc                                                                                                               >  2:682179/1‑107 (MQ=255)
                                                                           tGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTAc                                                                                                               <  1:682179/107‑1 (MQ=255)
                                                                            gAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTcc                                                                    <  1:71076/149‑1 (MQ=255)
                                                                               gcgcAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACCCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTa                                                                 <  4:430805/149‑1 (MQ=255)
                                                                                    aaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAAt                                                                                                                                       <  2:796456/74‑1 (MQ=255)
                                                                                    aaGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTc                                                            >  3:282237/1‑149 (MQ=255)
                                                                                      gagTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTcc                                                                    >  1:574156/1‑139 (MQ=255)
                                                                                      gagTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTACTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCt                                                          <  4:469726/149‑1 (MQ=255)
                                                                                         tttATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtggtg                                                                                                                        >  3:467538/1‑84 (MQ=255)
                                                                                         tttATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtgg                                                                                                                          <  4:821604/82‑1 (MQ=255)
                                                                                         tttATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTgtgg                                                                                                                          >  3:821604/1‑82 (MQ=255)
                                                                                           tATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                            <  1:196976/78‑1 (MQ=255)
                                                                                           tATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                            >  2:196976/1‑78 (MQ=255)
                                                                                             ttGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTg                                                   >  1:1263018/1‑149 (MQ=255)
                                                                                              tGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                  <  4:638283/149‑1 (MQ=255)
                                                                                              tGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                  <  1:937358/149‑1 (MQ=255)
                                                                                               gAGGCGGCACAAGTTGGCGGTGTATCGACATCGGCCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCAtaa                                                                                      <  1:1348301/112‑3 (MQ=255)
                                                                                                  gcggcACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                            >  2:512520/1‑71 (MQ=255)
                                                                                                  gcggcACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgtgt                                                                                                                            <  1:512520/71‑1 (MQ=255)
                                                                                                  gcggcACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTAc                                              <  4:622260/149‑1 (MQ=255)
                                                                                                       acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGtggtggtgg                                                                                                                    <  1:842044/74‑1 (MQ=255)
                                                                                                       acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGtggtggtgg                                                                                                                    >  2:842044/1‑74 (MQ=255)
                                                                                                       acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCt                                                                                                                 <  3:805828/77‑1 (MQ=255)
                                                                                                       acaAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCt                                                                                                                 >  4:805828/1‑77 (MQ=255)
                                                                                                        caAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCgg                                                                                                                               <  4:882914/62‑1 (MQ=255)
                                                                                                        caAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCgg                                                                                                                               >  3:882914/1‑62 (MQ=255)
                                                                                                        caAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAaga                                        <  3:1232786/149‑1 (MQ=255)
                                                                                                          aGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGgt                                                                                                                              <  2:1269821/61‑1 (MQ=255)
                                                                                                          aGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGc                                      >  3:1211133/1‑149 (MQ=255)
                                                                                                            ttGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCAGTGTGGTGGTGGTCTAc                                                                                                               >  1:1034773/1‑74 (MQ=255)
                                                                                                            ttGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCAGTGTGGTGGTGGTCTAc                                                                                                               <  2:1034773/74‑1 (MQ=255)
                                                                                                                  gtgtATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGAGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCTTTAagc                              >  4:1459720/1‑149 (MQ=255)
                                                                                                                      aTCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                  <  1:263184/125‑1 (MQ=255)
                                                                                                                      aTCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                  >  2:263184/1‑125 (MQ=255)
                                                                                                                       tCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTg                         >  4:1323315/1‑149 (MQ=255)
                                                                                                                         gACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTggg                       <  4:1211133/149‑1 (MQ=255)
                                                                                                                               gggCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                  >  3:302056/1‑116 (MQ=255)
                                                                                                                               gggCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTgg                                                  <  4:302056/116‑1 (MQ=255)
                                                                                                                                 gCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGATTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTg               <  3:245495/149‑1 (MQ=255)
                                                                                                                                  cATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTgggg                                                      >  3:1388326/1‑109 (MQ=255)
                                                                                                                                  cATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCAAGATCCTCTTTGAATCTTTCCTTAGCTTCCTgggg                                                      <  4:1388326/109‑1 (MQ=255)
                                                                                                                                    ttGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAaga                                        >  1:1158141/1‑121 (MQ=255)
                                                                                                                                    ttGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAaga                                        <  2:1158141/121‑1 (MQ=255)
                                                                                                                                    ttGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGtt                                  <  3:57898/127‑1 (MQ=255)
                                                                                                                                      gTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAg          <  3:1323315/149‑1 (MQ=255)
                                                                                                                                       ttATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGccc                                                                                             >  4:34427/1‑65 (MQ=255)
                                                                                                                                       ttATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTcc                                                                    >  2:1435364/1‑90 (MQ=255)
                                                                                                                                         aTTCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTg                                                                             >  2:120832/1‑79 (MQ=39)
                                                                                                                                         aTTCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGa       >  1:432919/1‑149 (MQ=255)
                                                                                                                                          ttCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAagcag                            >  2:606130/1‑127 (MQ=255)
                                                                                                                                          ttCGGCACAATGTGGCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGAt      >  3:931281/1‑149 (MQ=255)
                                                                                                                                           tCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGATGCATTGCTGAGTGATg     >  4:189738/1‑149 (MQ=255)
                                                                                                                                                acaTTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCtt                                                                  <  4:214893/83‑1 (MQ=255)
                                                                                                                                                   ttGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGa           >  1:1384467/1‑135 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGCTGATTTTCGTGGCGATAGGCATGGTTTCCTGGCTGGATATGGCTCGTATTGTGCGTGGGCAAACCCTGAGTCTGAAGCGCAAAGAGTTTATTGAGGCGGCACAAGTTGGCGGTGTATCGACATCGGGCATTGTTATTCGCCACATTGTGCCGAATGTACTCGGTGTGGTGGTGGTCTACGCATCACTTCTGGTGCCCAGCATGATCCTCTTTGAATCTTTCCTTAGCTTCCTGGGGTTGGGTACGCAAGAGCCGTTAAGCAGCTGGGGTGCATTGCTGAGTGATGGCG  >  NC_000913/1304327‑1304617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: