Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,388,284 | A→T | 31.2% | intergenic (+23/+21) | tyrR → / ← tpx | aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,388,284 | 0 | A | T | 31.2% | 70.4 / 43.7 | 48 | intergenic (+23/+21) | tyrR/tpx | aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase |
Reads supporting (aligned to +/- strand): ref base A (16/17); new base T (6/9); total (22/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
CTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCC > NC_000913/1388145‑1388422 | cTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAGGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCa < 1:870467/149‑1 (MQ=255) cTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCa < 4:1413468/149‑1 (MQ=255) cTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCa < 3:1385561/149‑1 (MQ=255) tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg < 3:484742/149‑1 (MQ=255) tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg < 3:141872/149‑1 (MQ=255) tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg < 4:381264/149‑1 (MQ=255) tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg < 2:600719/149‑1 (MQ=255) tCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg < 3:1362949/145‑1 (MQ=255) tCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg > 4:1362949/1‑145 (MQ=255) aaTTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATc > 1:201544/1‑139 (MQ=255) aaTTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATc < 2:201544/139‑1 (MQ=255) ccAGCACGCGCAAACTGGCAAAACTTCTCGGCGTTTCACATACCGCGAGTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCa > 2:324791/1‑133 (MQ=255) ccAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCa < 1:324791/133‑1 (MQ=255) gCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg > 2:499512/1‑131 (MQ=255) gCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg < 1:499512/131‑1 (MQ=255) gcAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATc < 3:62587/123‑1 (MQ=255) gcAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATc > 4:62587/1‑123 (MQ=255) aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAATACCATCAGGCatat < 3:100503/121‑1 (MQ=255) aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCatat > 4:100503/1‑121 (MQ=255) tctcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt < 4:274639/106‑1 (MQ=255) tctcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt > 3:274639/1‑106 (MQ=255) tCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg < 3:777777/99‑1 (MQ=255) tCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg > 4:777777/1‑99 (MQ=255) acaTACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc > 4:745387/1‑125 (MQ=255) acaTACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc < 3:745387/125‑1 (MQ=255) cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATc < 3:1417732/80‑1 (MQ=38) cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATc > 4:1417732/1‑80 (MQ=38) gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa > 4:1050942/1‑146 (MQ=255) gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa < 3:1050942/146‑1 (MQ=255) tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt > 3:912149/1‑58 (MQ=255) tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt > 3:218033/1‑58 (MQ=255) tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt < 4:218033/58‑1 (MQ=255) tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt < 4:912149/58‑1 (MQ=255) agaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt < 2:1215459/66‑1 (MQ=255) agaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCttt > 1:1215459/1‑66 (MQ=255) aaTATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa < 1:1387471/112‑1 (MQ=255) aaTATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa > 2:358432/1‑112 (MQ=255) aaTATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa > 2:1387471/1‑112 (MQ=255) aaTATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa < 1:358432/112‑1 (MQ=255) ccTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGc > 3:1351854/1‑149 (MQ=255) cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc > 3:803730/1‑149 (MQ=255) cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGcc > 3:1332108/1‑149 (MQ=255) cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtg > 3:662733/1‑77 (MQ=255) cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtg < 4:662733/77‑1 (MQ=255) gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggt > 3:776835/1‑74 (MQ=255) gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggt < 4:776835/74‑1 (MQ=255) cAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACAtt < 3:42707/103‑1 (MQ=255) cAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACAtt > 4:42707/1‑103 (MQ=255) | CTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCC > NC_000913/1388145‑1388422 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |