| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,410,986 | A→G | 9.8% | intergenic (+102/+27) | dbpA → / ← ttcA | ATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,410,986 | 0 | A | G | 9.8% | 153.5 / 7.1 | 51 | intergenic (+102/+27) | dbpA/ttcA | ATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein |
| Reads supporting (aligned to +/- strand): ref base A (24/22); new base G (3/2); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01 | |||||||||||
GAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTT > NC_000913/1410853‑1411132 | gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCggg > 2:1255282/1‑149 (MQ=255) aaaaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGgc < 1:1255282/149‑1 (MQ=255) taatGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgc < 2:604956/112‑1 (MQ=255) taatGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgc > 1:604956/1‑112 (MQ=255) tGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCggg > 3:1367139/1‑117 (MQ=38) tGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCggg < 4:1367139/117‑1 (MQ=38) agagTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACcaca > 1:141218/1‑123 (MQ=255) agagTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACcaca < 2:141218/123‑1 (MQ=255) gTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAAc > 4:529040/1‑132 (MQ=255) gTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAAc < 3:529040/132‑1 (MQ=255) aCATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTg < 4:573943/96‑1 (MQ=255) aCATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTg > 3:573943/1‑96 (MQ=255) tCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATc > 1:97477/1‑80 (MQ=255) tCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATc < 2:97477/80‑1 (MQ=255) gATTGCCGGATGCGACTCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGc > 4:335963/1‑61 (MQ=255) gATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGc < 3:335963/61‑1 (MQ=255) gCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAt > 3:764670/1‑123 (MQ=255) gCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAt < 4:764670/123‑1 (MQ=255) gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGa > 2:388115/1‑107 (MQ=255) gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGa < 1:388115/107‑1 (MQ=255) gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTGATCTTCTTCTGGCTGCCAGCACGCCgg < 3:975337/134‑1 (MQ=255) gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCgg > 4:975337/1‑134 (MQ=255) tATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtct > 4:670611/1‑107 (MQ=255) tATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtct < 3:670611/107‑1 (MQ=255) ggCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc > 1:1256980/1‑149 (MQ=255) gCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAtctc < 2:1407259/139‑1 (MQ=255) gCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAtctc > 1:1407259/1‑139 (MQ=255) cTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttctt < 3:118696/103‑1 (MQ=255) cTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttctt > 4:118696/1‑103 (MQ=255) cATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTGTTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAtt < 3:897920/89‑1 (MQ=255) cATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAtt > 4:897920/1‑89 (MQ=255) aTAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGt > 2:863901/1‑125 (MQ=255) aTAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGt < 1:863901/125‑1 (MQ=255) aTAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt > 1:1251951/1‑130 (MQ=255) aTAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt < 2:1251951/130‑1 (MQ=255) aaGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCa < 4:1041189/148‑1 (MQ=255) aaGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCa > 3:1041189/1‑148 (MQ=255) aGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcc > 1:1159743/1‑149 (MQ=255) gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTa > 1:433526/1‑114 (MQ=255) gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTa < 2:433526/114‑1 (MQ=255) cccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt < 3:848854/44‑1 (MQ=255) cccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt > 4:848854/1‑44 (MQ=255) ccGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAccccc > 4:1174904/1‑149 (MQ=255) ccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt > 4:368617/1‑119 (MQ=255) ccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt < 3:368617/119‑1 (MQ=255) cGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCg > 1:1184814/1‑149 (MQ=255) gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGt < 4:461120/149‑1 (MQ=255) gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGt > 3:461120/1‑149 (MQ=255) aTACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGtt < 2:1159743/149‑1 (MQ=255) tACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGtt > 4:1314244/1‑105 (MQ=255) tACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGtt < 3:1314244/105‑1 (MQ=255) | GAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTT > NC_000913/1410853‑1411132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |