Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,419,624 G→A 100% intergenic (‑168/+141) ydaG ← / ← racR Rac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,419,6240GA95.9% 511.0 / 7.0 145intergenic (‑168/+141)ydaG/racRRac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base A (64/75);  total (66/79)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 155 total aligned reads displayed.

CCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAG  >  NC_000913/1419476‑1419769
                                                                                                                                                    |                                                                                                                                                 
ccGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTgg                                                                                                                                                   >  4:1256006/1‑149 (MQ=255)
  gggTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGaa                                                                                                                                                 <  3:329996/149‑1 (MQ=255)
      ggCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACtat                                                                                                                                             <  1:1072285/149‑1 (MQ=255)
       gCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACtata                                                                                                                                            <  3:1278713/149‑1 (MQ=255)
        cGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAg                                                                                                                                           >  1:76518/1‑149 (MQ=255)
        cGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAg                                                                                                                                           <  4:441024/149‑1 (MQ=255)
         ggAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGaaa                                                                                                                                                 <  4:1343040/142‑1 (MQ=255)
         ggAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGaaa                                                                                                                                                 >  3:1343040/1‑142 (MQ=255)
                    ctgcactgcaAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAat                                                                                                                               <  2:1069238/149‑1 (MQ=255)
                     tgcactgcaAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGaaa                                                                                                                                                 <  1:6414/130‑1 (MQ=255)
                     tgcactgcaAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGaaa                                                                                                                                                 >  3:86031/1‑130 (MQ=255)
                       cactgcaAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattga                                                                                                                            >  3:875866/1‑149 (MQ=255)
                        actgcaAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgat                                                                                                                           <  4:112536/149‑1 (MQ=255)
                        actgcaAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgat                                                                                                                           <  2:613471/149‑1 (MQ=255)
                                      aTCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTAt                                                                                                             >  3:750382/1‑149 (MQ=255)
                                       tCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGaaa                                                                                                                                                 >  1:352827/1‑112 (MQ=255)
                                       tCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATg                                                                                                            <  2:1229169/149‑1 (MQ=255)
                                         ccgccgTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattga                                                                                                                            >  3:111281/1‑131 (MQ=255)
                                         ccgccgTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattga                                                                                                                            <  4:111281/131‑1 (MQ=255)
                                         ccgccgTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGt                                                                                                                        <  2:947859/135‑1 (MQ=255)
                                           gccgTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTa                                                                                                        >  4:1182622/1‑149 (MQ=255)
                                            ccgTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTaa                                                                                                       <  1:371725/149‑1 (MQ=255)
                                            ccgTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTaa                                                                                                       <  3:1182622/149‑1 (MQ=255)
                                                 aTGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgat                                                                                                                           <  2:430165/124‑1 (MQ=255)
                                                 aTGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgat                                                                                                                           >  1:430165/1‑124 (MQ=255)
                                                 aTGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGttt                                                                                                  <  2:1369969/149‑1 (MQ=255)
                                                   gTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCa                                                                                                                      <  4:832098/127‑1 (MQ=255)
                                                    ttCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGttt                                                                                                  >  2:46036/1‑146 (MQ=255)
                                                    ttCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               <  2:76518/149‑1 (MQ=255)
                                                    ttCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               >  3:1225954/1‑149 (MQ=255)
                                                    ttCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               >  2:362459/1‑149 (MQ=255)
                                                       aTACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               <  4:949249/146‑1 (MQ=255)
                                                       aTACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               >  3:949249/1‑146 (MQ=255)
                                                        tACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgatt                                                                                                                          <  4:168938/118‑1 (MQ=255)
                                                        tACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTaa                                                                                                       >  1:315108/1‑137 (MQ=255)
                                                         aCGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACCCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGt                                                                                                                        >  4:711758/1‑119 (MQ=255)
                                                         aCGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACCCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGt                                                                                                                        <  3:711758/119‑1 (MQ=255)
                                                          cGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTaa                                                                                         <  4:731835/149‑1 (MQ=255)
                                                             cTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGttt                                                                                                  <  1:150191/137‑1 (MQ=255)
                                                             cTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCt                                                                                      >  4:1324958/1‑149 (MQ=255)
                                                             cTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCt                                                                                      >  1:1419542/1‑149 (MQ=255)
                                                               cGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTaa                                                                                    <  2:1419542/149‑1 (MQ=255)
                                                                      gctACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTaa                                                                                                                                       >  1:661876/1‑91 (MQ=255)
                                                                      gctACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTaa                                                                                                                                       <  2:661876/91‑1 (MQ=255)
                                                                      gctACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGa                                                                             <  3:1130021/149‑1 (MQ=255)
                                                                      gctACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGa                                                                             <  4:1331216/149‑1 (MQ=255)
                                                                        tACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATa                                                                                                                   >  2:1427525/1‑109 (MQ=255)
                                                                        tACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATa                                                                                                                   <  1:1427525/109‑1 (MQ=255)
                                                                        tACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTaa                                                                                                       <  1:1289326/121‑1 (MQ=255)
                                                                        tACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGtttt                                                                                                 <  1:677970/127‑1 (MQ=255)
                                                                            tAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGa                                                                       <  1:52043/149‑1 (MQ=255)
                                                                            tAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGa                                                                       <  3:1324958/149‑1 (MQ=255)
                                                                             aaCCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAAt                                                                                                                                      <  4:66096/85‑1 (MQ=255)
                                                                             aaCCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAAt                                                                                                                                      >  3:66096/1‑85 (MQ=255)
                                                                                cccTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgatt                                                                                                                          >  4:1442867/1‑94 (MQ=255)
                                                                                cccTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgatt                                                                                                                          <  3:1442867/94‑1 (MQ=255)
                                                                                  cTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGtt                                                                                                   <  3:1338488/115‑1 (MQ=255)
                                                                                  cTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGAcagcag                                                                 >  3:278071/1‑149 (MQ=255)
                                                                                   ttACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               <  2:1235898/118‑1 (MQ=255)
                                                                                   ttACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               >  1:1235898/1‑118 (MQ=255)
                                                                                     aCCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTaa                                                                                    >  1:1033972/1‑127 (MQ=255)
                                                                                     aCCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTaa                                                                                    <  2:1033972/127‑1 (MQ=255)
                                                                                     aCCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGaaa                                                              <  2:537463/149‑1 (MQ=255)
                                                                                           gCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATg                                                        <  4:29742/149‑1 (MQ=255)
                                                                                             cTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATggg                                                      >  1:530577/1‑149 (MQ=255)
                                                                                             cTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATggg                                                      >  1:1301321/1‑149 (MQ=255)
                                                                                              tGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAattgatt                                                                                                                          <  2:1242743/80‑1 (MQ=255)
                                                                                              tGGTAACGCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTCATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATgcgg                                                     >  2:975856/1‑149 (MQ=255)
                                                                                               ggTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATgggg                                                     >  1:786681/1‑148 (MQ=255)
                                                                                                gTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATgggggg                                                   >  1:187019/1‑149 (MQ=255)
                                                                                                    cTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTa                                               >  2:29807/1‑149 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATggg                                                      <  2:25050/141‑1 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATggg                                                      >  1:25050/1‑141 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTaa                                                                                                       >  4:1057846/1‑92 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGtttt                                                                                                 >  3:251213/1‑98 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGtttt                                                                                                 <  4:671088/98‑1 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGtttt                                                                                                 <  4:251213/98‑1 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAAcc                                                                                       <  3:149688/108‑1 (MQ=255)
                                                                                                     tCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAt                                                         >  2:334209/1‑138 (MQ=255)
                                                                                                          gTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAAc                                         <  4:1225954/149‑1 (MQ=255)
                                                                                                             ttGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGa                                      <  2:530577/149‑1 (MQ=255)
                                                                                                                cccGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGa                                                          <  2:711118/126‑1 (MQ=255)
                                                                                                                 ccGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCATAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGaaa                                                              <  2:294985/121‑1 (MQ=255)
                                                                                                                  cGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCa                                 <  2:618651/149‑1 (MQ=255)
                                                                                                                  cGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCa                                 >  3:1114088/1‑149 (MQ=255)
                                                                                                                   ggCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAg                                <  1:29807/149‑1 (MQ=255)
                                                                                                                     cGTTCTGTGGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGa                              <  4:894763/149‑1 (MQ=255)
                                                                                                                          tgtgGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGtttt                         >  1:697898/1‑149 (MQ=255)
                                                                                                                            tgGGGCGGGGTGGGTGGTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTcc                       <  4:875866/149‑1 (MQ=255)
                                                                                                                              gggCGGGGTGGGTGGTATGCTGAAGCTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTcc                       >  1:1241680/1‑147 (MQ=255)
                                                                                                                                     gtgggtggTATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGa                                                                                               <  2:196599/68‑1 (MQ=255)
                                                                                                                                            gtATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAg                                                             <  1:538349/95‑1 (MQ=255)
                                                                                                                                            gtATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGa                              >  2:358017/1‑126 (MQ=255)
                                                                                                                                            gtATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGa                              <  1:358017/126‑1 (MQ=255)
                                                                                                                                            gtATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagt       <  2:1301321/149‑1 (MQ=255)
                                                                                                                                             tATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATggg                                                      <  2:668689/101‑1 (MQ=255)
                                                                                                                                             tATGCTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtt      <  2:697898/149‑1 (MQ=255)
                                                                                                                                                 cTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGa                                                                             >  4:303835/1‑74 (MQ=255)
                                                                                                                                                 cTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAg                  >  4:808573/1‑133 (MQ=255)
                                                                                                                                                 cTGAAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAg                  <  3:808573/133‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAG  >  NC_000913/1419476‑1419769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: