Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,462,028 G→A 6.3% G379D (GGT→GAT) ‡ paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,462,0280GA 6.3% 621.7 / 21.5 188G379D (GGT→GAT) ‡paaJ3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (88/88);  new base A (8/4);  total (96/92)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 196 total aligned reads displayed.

CGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGT  >  NC_000913/1461881‑1462176
                                                                                                                                                   |                                                                                                                                                    
cGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGt                                                                                                                                                     <  1:758614/149‑1 (MQ=255)
     ggTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTa                                                                                                                                                <  3:30998/149‑1 (MQ=255)
      gtgtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                               <  1:291694/149‑1 (MQ=255)
             acGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATtgt                                                                                                                                        >  1:527341/1‑149 (MQ=255)
                   atgggggGTGCCTGATGATGCCCCACATCTTTACCGCAACGGAGGCGGTATCCTGGTAGGGCACCACTTGGGAATCACAGGTGCCGGCCTAGCACTTTCTGCCAGCCATGGGGTATGTGGGGGTAACGGCCGTTACTCATTGTGAACCa                                                                                                                                  >  4:904042/4‑149 (MQ=255)
                         gCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgt                                                                                                                               >  2:886971/1‑146 (MQ=255)
                         gCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGGCGTTACGCATTGTGCACCAtgt                                                                                                                               <  1:886971/146‑1 (MQ=255)
                              cTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGgt                                                                                                                       <  3:904042/149‑1 (MQ=255)
                               tgatgaTGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGt                                                                                                                                                     <  3:1452638/118‑1 (MQ=255)
                               tgatgaTGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGt                                                                                                                                                     >  4:1452638/1‑118 (MQ=255)
                                    atgCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgt                                                                                                                               <  4:5202/135‑1 (MQ=255)
                                    atgCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgt                                                                                                                               >  3:5202/1‑135 (MQ=255)
                                      gCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCCCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCa                                                                                                               >  2:606906/1‑149 (MQ=255)
                                       ccccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAg                                                                                                              <  3:202205/149‑1 (MQ=255)
                                         ccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                            >  3:1292212/1‑149 (MQ=255)
                                         ccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                            >  3:1152315/1‑149 (MQ=255)
                                         ccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                            >  2:153645/1‑149 (MQ=255)
                                           acaTGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCa                                                                                                          <  4:1292212/149‑1 (MQ=255)
                                                ttAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGcc                                                                                                     >  4:313342/1‑149 (MQ=255)
                                                ttAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGcc                                                                                                     >  1:336745/1‑149 (MQ=255)
                                                     cccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAt                                                                                                >  3:506625/1‑149 (MQ=255)
                                                      ccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAtt                                                                                               >  1:1043560/1‑149 (MQ=255)
                                                      ccAACGGAGGCGCTATCGCCTTAGGCCATCCGTGGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAtt                                                                                               >  2:618778/1‑149 (MQ=255)
                                                      ccAACGGAGGCGCTATCGCCTTAAGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGGCGGTCAGGGCATCGCCATGAt                                                                                                >  2:492769/1‑148 (MQ=255)
                                                       cAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTc                                                                                              >  3:1060320/1‑149 (MQ=255)
                                                               gcgcTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgt                                                                                      <  1:618778/149‑1 (MQ=255)
                                                                   tATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGttt                                                                                  >  4:757409/1‑149 (MQ=255)
                                                                    aTCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTAc                                                                                                                                               >  3:1109541/1‑87 (MQ=255)
                                                                     tCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCg                                                                                                                   <  1:240000/114‑1 (MQ=255)
                                                                     tCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCg                                                                                                                   >  2:240000/1‑114 (MQ=255)
                                                                      cGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAg                                                                               <  4:1099993/149‑1 (MQ=255)
                                                                       gCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGc                                                                              <  1:790356/149‑1 (MQ=255)
                                                                       gCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGGGCATCGGTGCCGGTCAGGGCATCGCCATGATTCTGGAGCGGGTTTGAGc                                                                              >  4:821985/1‑149 (MQ=255)
                                                                         cTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGttt                                                                                  >  2:766614/1‑143 (MQ=255)
                                                                         cTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCat                                                                            <  2:334955/149‑1 (MQ=255)
                                                                         cTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCat                                                                            >  1:695559/1‑149 (MQ=255)
                                                                              gCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTa                                                                                                                                                >  4:800289/1‑76 (MQ=255)
                                                                               gcctCCGTTGGGGATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAc                                                                      <  2:729923/146‑1 (MQ=255)
                                                                                 aTCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGtt                                                                                   >  1:1240443/1‑134 (MQ=255)
                                                                                 aTCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCa                                                                             <  1:160360/140‑1 (MQ=255)
                                                                                 aTCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCt                                                                    <  2:455816/149‑1 (MQ=255)
                                                                                  tCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTg                                                                   >  2:529619/1‑149 (MQ=255)
                                                                                   ccGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAgg                                                                                                             >  4:1137612/1‑106 (MQ=255)
                                                                                     gTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCg                                                                                                                         >  2:508062/1‑92 (MQ=255)
                                                                                           aaTGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGa                                                                                                 <  4:376966/110‑1 (MQ=255)
                                                                                             tGAGTGGTGCCCCCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTa                                                        <  1:606906/149‑1 (MQ=255)
                                                                                              gAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGt                                                                                                                                                  <  4:1399394/58‑1 (MQ=255)
                                                                                              gAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGt                                                                                                                                                  >  3:1399394/1‑58 (MQ=255)
                                                                                                 tggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGc                                                                                                                                      >  1:1211122/1‑67 (MQ=255)
                                                                                                  ggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCg                                                                                                                         >  1:669604/1‑79 (MQ=255)
                                                                                                  ggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCg                                                                                                                         <  2:669604/79‑1 (MQ=255)
                                                                                                  ggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCg                                                                                                                         <  2:681134/79‑1 (MQ=255)
                                                                                                  ggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCg                                                                                                                         >  1:681134/1‑79 (MQ=255)
                                                                                                   gtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGc                                                                                                                                      >  2:207999/1‑65 (MQ=255)
                                                                                                   gtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGc                                                                                                                                      <  1:207999/65‑1 (MQ=255)
                                                                                                   gtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGa                                                  >  3:579186/1‑149 (MQ=255)
                                                                                                     gCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                                  <  2:1100536/131‑1 (MQ=255)
                                                                                                         gCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGc                                                                              <  2:373229/115‑1 (MQ=255)
                                                                                                         gCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCa                                            <  2:973955/149‑1 (MQ=255)
                                                                                                          ccTGGCAATGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCaa                                           <  3:821985/149‑1 (MQ=255)
                                                                                                             ggCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATAc                                        <  2:863523/149‑1 (MQ=255)
                                                                                                               cACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGc                                                                                                           >  2:301601/1‑80 (MQ=255)
                                                                                                               cACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACaa                                      <  1:456320/149‑1 (MQ=255)
                                                                                                                aCTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACaaa                                     <  1:50129/149‑1 (MQ=255)
                                                                                                                   ggCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCt                                  >  1:1194516/1‑149 (MQ=255)
                                                                                                                    gCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCg                                                                                                                         >  1:314883/1‑61 (MQ=255)
                                                                                                                      tGCCAGCCATGTGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGa                               <  1:999885/149‑1 (MQ=255)
                                                                                                                      tGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGa                               <  4:503714/149‑1 (MQ=255)
                                                                                                                        ccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCAt                                                                                                   <  1:1320365/79‑1 (MQ=255)
                                                                                                                         cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGa                                                                                          <  3:344909/87‑1 (MQ=255)
                                                                                                                         cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACaa                                                     >  1:406409/1‑124 (MQ=255)
                                                                                                                         cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCaa                                           >  3:1438841/1‑134 (MQ=255)
                                                                                                                         cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCaa                                           <  4:1438841/134‑1 (MQ=255)
                                                                                                                             caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAtt                        <  4:579186/149‑1 (MQ=255)
                                                                                                                                gAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGa                                                                <  2:1291045/106‑1 (MQ=255)
                                                                                                                                 aGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGaaa                    <  2:695559/149‑1 (MQ=255)
                                                                                                                                    tGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTg                                                                                            >  3:1249322/1‑74 (MQ=255)
                                                                                                                                    tGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTg                                                                                            <  4:1249322/74‑1 (MQ=255)
                                                                                                                                        tCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGa                                                                                          <  1:1271539/72‑1 (MQ=255)
                                                                                                                                        tCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGa                                                                                          >  2:1266141/1‑72 (MQ=255)
                                                                                                                                        tCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTc             >  3:869262/1‑149 (MQ=255)
                                                                                                                                        aatgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAGCCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTc             >  3:1387275/4‑149 (MQ=255)
                                                                                                                                        aatgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGa                          >  3:387079/4‑136 (MQ=255)
                                                                                                                                          tgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAAt                                                    <  4:100558/107‑1 (MQ=255)
                                                                                                                                          tgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAAt                                                    >  3:100558/2‑108 (MQ=255)
                                                                                                                                           gCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCATCCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGaa                         <  2:1239246/134‑2 (MQ=255)
                                                                                                                                              tAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtga       >  4:557007/1‑149 (MQ=255)
                                                                                                                                              tAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtga       >  4:1153112/1‑149 (MQ=255)
                                                                                                                                               aaCGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgc                <  1:1015522/139‑1 (MQ=255)
                                                                                                                                               aaCGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgat      <  3:757409/149‑1 (MQ=255)
                                                                                                                                               aaCGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgat      <  1:529619/149‑1 (MQ=255)
                                                                                                                                               aaCGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgat      <  3:794287/149‑1 (MQ=255)
                                                                                                                                               aaCGGTCGATACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgat      <  2:1194516/149‑1 (MQ=255)
                                                                                                                                                 cGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgatga    <  3:557007/149‑1 (MQ=255)
                                                                                                                                                  ggTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATCTCAACCTGCGAGTACCCTACAATGATAACCAATa                                         >  2:1136094/1‑111 (MQ=255)
                                                                                                                                                  ggTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                                  <  4:277113/86‑1 (MQ=255)
                                                                                                                                                  ggTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATa                                         <  1:1136094/111‑1 (MQ=255)
                                                                                                                                                  ggTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgat      <  1:824660/146‑1 (MQ=255)
                                                                                                                                                   gTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGt  >  4:40504/1‑149 (MQ=255)
                                                                                                                                                   gTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGt  >  4:1039536/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGT  >  NC_000913/1461881‑1462176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: