Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,483,343 G→C 10.0% V95L (GTG→CTG)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,483,3430GC10.0% 380.7 / 28.4 110V95L (GTG→CTG) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (49/50);  new base C (6/5);  total (55/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

Only 100 of 112 total aligned reads displayed.

CATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCG  >  NC_000913/1483195‑1483489
                                                                                                                                                    |                                                                                                                                                  
cATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAgg                                                                                                                                                    >  3:600238/1‑149 (MQ=255)
  ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtg                                                                                                                                                  <  1:130690/149‑1 (MQ=255)
      ccAGGAGATGGCGTAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                                                              <  4:925666/149‑1 (MQ=255)
      ccAGGAGATGGCGAAAGAGATTGACCAGGCGTCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGa                                                                                                                                              <  1:418490/149‑1 (MQ=255)
        aGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtc                                                                                                                                            <  2:958258/149‑1 (MQ=255)
        aGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAtc                                                                                                                                            >  4:592573/1‑149 (MQ=255)
                  gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCgggg                                                                                                                                  <  1:199531/149‑1 (MQ=255)
                  gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCgggg                                                                                                                                  <  1:31623/149‑1 (MQ=255)
                  gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           >  4:1231266/1‑139 (MQ=255)
                  gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           <  3:1231266/140‑2 (MQ=255)
                     agagATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGaaa                                                                                                                               >  4:1107748/1‑149 (MQ=255)
                           tGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGtt                                                                                                                         <  3:592573/149‑1 (MQ=255)
                            gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        <  4:600238/149‑1 (MQ=255)
                               cAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTgg                                                                                                                     <  3:5858/149‑1 (MQ=255)
                                aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        >  2:87189/1‑145 (MQ=255)
                                aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        <  1:87189/145‑1 (MQ=255)
                                     gcAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAAc                                                                                                               <  2:377908/149‑1 (MQ=255)
                                       aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                                                                             <  3:1107748/149‑1 (MQ=255)
                                       aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCATGATCACCAGGTGGTGAtcgtc                                                                                                                                         >  2:1360673/1‑121 (MQ=255)
                                       aGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCATGATCACCAGGTGGTGAtcgtc                                                                                                                                         <  1:1360673/121‑1 (MQ=255)
                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAAc                                                                                                                              >  4:485503/1‑131 (MQ=255)
                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAAc                                                                                                                              >  4:376851/1‑131 (MQ=255)
                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAAc                                                                                                                              <  3:376851/131‑1 (MQ=255)
                                        gggAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAAc                                                                                                                              <  3:485503/131‑1 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           >  2:472529/1‑112 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           <  3:1343172/113‑2 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           >  4:1343172/1‑112 (MQ=255)
                                             aGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAtca                                                                                                                                           <  1:472529/113‑2 (MQ=255)
                                              gTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAAc                                                                                                               <  3:262486/140‑1 (MQ=255)
                                              gTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAAc                                                                                                               >  4:262486/1‑140 (MQ=255)
                                                   gctgcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                                                                    <  1:916866/146‑1 (MQ=255)
                                                   gctgcGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                                                                    >  2:916866/1‑146 (MQ=255)
                                                           aaGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAAc                                                                                                                              >  2:1385309/1‑112 (MQ=255)
                                                           aaGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAAc                                                                                                                              <  1:1385309/112‑1 (MQ=255)
                                                            aGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                                                                        <  4:1460460/149‑1 (MQ=255)
                                                               ggCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACgg                                                                                                                            >  4:201593/1‑110 (MQ=255)
                                                               ggCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACgg                                                                                                                            <  3:201593/110‑1 (MQ=255)
                                                                   cGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCt                                                                                 >  2:810038/1‑149 (MQ=255)
                                                                       cGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgggg                                                                             >  3:971187/1‑149 (MQ=255)
                                                                           aaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGcgcg                                                                         >  2:1060724/1‑149 (MQ=255)
                                                                                 ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGaaa                                                                                                                               >  1:271890/1‑89 (MQ=255)
                                                                                 ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                                                                   >  3:828679/1‑149 (MQ=255)
                                                                                  tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaa                                                                  <  4:828679/149‑1 (MQ=255)
                                                                                   aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 <  4:971187/149‑1 (MQ=255)
                                                                                      cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  4:1062055/149‑1 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        <  4:691816/89‑1 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTc                                                                                                                        >  3:691816/1‑89 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                                                                            >  3:1409476/1‑117 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                                                                            <  4:1409476/117‑1 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCt                                                                                           <  4:505945/118‑1 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 <  3:48052/144‑1 (MQ=255)
                                                                                        gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 >  4:48052/1‑144 (MQ=255)
                                                                                                aCCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTg                                                                                                                      >  2:647081/1‑83 (MQ=255)
                                                                                                aCCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTg                                                                                                                      <  1:647081/83‑1 (MQ=255)
                                                                                                  cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              >  1:1045983/1‑137 (MQ=255)
                                                                                                  cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGCGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  2:1045983/137‑1 (MQ=255)
                                                                                                    gTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg                                                       >  1:1289330/1‑142 (MQ=255)
                                                                                                    gTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg                                                       <  2:1289330/142‑1 (MQ=255)
                                                                                                     ttAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGAACACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATAcc                                               <  1:810038/149‑1 (MQ=255)
                                                                                                        gTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAg                                            >  2:532106/1‑149 (MQ=255)
                                                                                                         tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 <  4:649441/127‑1 (MQ=255)
                                                                                                         tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaaa                                                                 >  3:649441/1‑127 (MQ=255)
                                                                                                         tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                                                <  4:858696/128‑1 (MQ=255)
                                                                                                         tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg                                                                >  3:858696/1‑128 (MQ=255)
                                                                                                         tCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGc                                           >  4:1459691/1‑149 (MQ=255)
                                                                                                           aGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                                                                >  1:849389/1‑78 (MQ=255)
                                                                                                           aGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                                                                                <  2:849389/78‑1 (MQ=255)
                                                                                                           aGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAt                                                                                                                                             <  2:1349831/49‑1 (MQ=38)
                                                                                                           aGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCACCTGGTGAt                                                                                                                                             >  1:1349831/1‑49 (MQ=38)
                                                                                                            gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGcgcg                                                                         >  3:121950/1‑116 (MQ=255)
                                                                                                            gAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGcgcg                                                                         <  4:121950/116‑1 (MQ=255)
                                                                                                                 aaCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGcgtcgt                                   >  1:339115/1‑149 (MQ=255)
                                                                                                                  aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              >  1:535447/1‑121 (MQ=255)
                                                                                                                  aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  2:535447/121‑1 (MQ=255)
                                                                                                                  aCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCgtcgtc                                  >  1:801323/1‑149 (MQ=255)
                                                                                                                      gACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  3:342670/117‑1 (MQ=255)
                                                                                                                      gACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              >  4:342670/1‑117 (MQ=255)
                                                                                                                         aTTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTggcggc                           >  2:979198/1‑149 (MQ=255)
                                                                                                                           tctcGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCaa                         <  3:1459691/149‑1 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCaa                         >  1:946249/1‑145 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCaa                         >  3:46787/1‑145 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCaa                         <  4:46787/145‑1 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCaa                         <  2:946249/145‑1 (MQ=255)
                                                                                                                               gAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAAc                     >  4:526333/1‑149 (MQ=255)
                                                                                                                                    gATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATa                                                 <  2:1434858/116‑1 (MQ=255)
                                                                                                                                    gATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATa                                                 >  1:1434858/1‑116 (MQ=255)
                                                                                                                                    gATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTgg                <  2:25172/149‑1 (MQ=255)
                                                                                                                                    gATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAAACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTgg                >  3:1099289/1‑149 (MQ=255)
                                                                                                                                     aTTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGc               >  3:1251293/1‑149 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                                                                    >  1:1437022/1‑75 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                                                                    <  2:1437022/75‑1 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCt                                 >  2:417297/1‑126 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCt                                 <  1:417297/126‑1 (MQ=255)
                                                                                                                                          tGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAAc                     >  1:787361/1‑138 (MQ=255)
                                                                                                                                           gATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              <  2:897612/96‑1 (MQ=255)
                                                                                                                                           gATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa                                                              >  1:897612/1‑96 (MQ=255)
                                                                                                                                           gATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGa                                                          <  3:250010/100‑1 (MQ=255)
                                                                                                                                           gATCACCACCTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGa                                                                      <  2:70129/88‑1 (MQ=255)
                                                                                                                                           gATCACCACCTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCg         >  3:1244578/1‑149 (MQ=255)
                                                                                                                                                  aGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCg  <  2:339115/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAACCGTATTGCG  >  NC_000913/1483195‑1483489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: