Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,603 C→A 7.4% V407V (GTG→GTT yddW ← liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,6030CA 7.4% 481.6 / 20.1 135V407V (GTG→GTTyddWliprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base C (66/59);  new base A (5/5);  total (71/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 135 total aligned reads displayed.

GTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCC  >  NC_000913/1567467‑1567750
                                                                                                                                        |                                                                                                                                                   
gTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTa                                                                                                                                         >  2:302416/1‑149 (MQ=255)
 tAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTaa                                                                                                                                        >  1:745446/1‑149 (MQ=255)
 tAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTaa                                                                                                                                        >  3:448599/1‑149 (MQ=255)
   aaGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGa                                                                                                                                      <  2:745446/149‑1 (MQ=255)
             ttGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGATTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGATGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAgctgct                                                                                                                            >  1:1190344/1‑149 (MQ=255)
                ttACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTaa                                                                                                                                        <  1:706734/134‑1 (MQ=255)
                ttACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTaa                                                                                                                                        >  2:706734/1‑134 (MQ=255)
                 tACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCttttt                                                                                                                        <  2:1190344/149‑1 (MQ=255)
                  aCAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCttttt                                                                                                                        >  4:377741/1‑148 (MQ=255)
                  aCAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCttttt                                                                                                                        <  3:377741/148‑1 (MQ=255)
                  aCAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCttttt                                                                                                                        >  4:784724/1‑148 (MQ=255)
                  aCAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCttttt                                                                                                                        <  3:784724/148‑1 (MQ=255)
                        tAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGtt                                                                                                                                          <  4:518860/124‑1 (MQ=255)
                         aaGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTcc                                                                                                                 <  4:703353/148‑1 (MQ=255)
                         aaGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTcc                                                                                                                 >  3:703353/1‑148 (MQ=255)
                          aGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCgg                                                                                                               >  3:323832/1‑149 (MQ=255)
                           gAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGt                                                                                                              >  3:816272/1‑149 (MQ=255)
                            aaTGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTcc                                                                                                                 <  4:351908/145‑1 (MQ=255)
                            aaTGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTcc                                                                                                                 >  3:351908/1‑145 (MQ=255)
                               gggAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGt                                                                                                                                           <  4:313695/116‑1 (MQ=255)
                               gggAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTAcgc                                                                                                          >  4:943388/1‑149 (MQ=255)
                                   gTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACgccgcc                                                                                                      >  3:663143/1‑149 (MQ=255)
                                         cTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAat                                                                                                >  2:239592/1‑149 (MQ=255)
                                           gCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatca                                                                                              <  2:594378/149‑1 (MQ=255)
                                            ccccAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatcat                                                                                             >  1:317210/1‑149 (MQ=255)
                                              ccAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTaa                                                                                                                                        >  4:1184968/1‑104 (MQ=255)
                                              ccAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGAt                                                                                                                                     >  1:873497/1‑107 (MQ=255)
                                                 gCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAgc                                                                                                                                >  4:284418/1‑109 (MQ=255)
                                                 gCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAgc                                                                                                                                <  3:284418/109‑1 (MQ=255)
                                                         gCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGctct                                                                                >  1:502550/1‑149 (MQ=255)
                                                            gATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATc                                                                             >  2:1421824/1‑149 (MQ=255)
                                                              tAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCtt                                                                           <  2:828507/149‑1 (MQ=255)
                                                               aGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCttt                                                                          <  1:1421824/149‑1 (MQ=255)
                                                                       gCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTc                                                                  >  1:94960/1‑149 (MQ=255)
                                                                        cTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCa                                                                 <  1:239592/149‑1 (MQ=255)
                                                                        cTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatcatc                                                                                            >  1:478041/1‑122 (MQ=255)
                                                                        cTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatcatc                                                                                            <  2:478041/122‑1 (MQ=255)
                                                                           gttgAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTcc                                                                                                                 <  4:1280412/98‑1 (MQ=255)
                                                                           gttgAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTcc                                                                                                                 >  3:1280412/1‑98 (MQ=255)
                                                                           gttgAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCgg                                                                                                               <  2:652575/100‑1 (MQ=255)
                                                                           gttgAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCgg                                                                                                               >  1:652575/1‑100 (MQ=255)
                                                                            ttgAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGa                                                                        <  2:999840/138‑1 (MQ=255)
                                                                            ttgAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGa                                                                        >  1:999840/1‑138 (MQ=255)
                                                                              gAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGaa                                                                       >  3:856648/1‑137 (MQ=255)
                                                                              gAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGaa                                                                       <  4:856648/137‑1 (MQ=255)
                                                                                   tGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCTCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTc                                                                                                                  <  3:999078/89‑1 (MQ=255)
                                                                                   tGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAgctgc                                                                                                                             >  3:490102/1‑78 (MQ=255)
                                                                                   tGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAgctgc                                                                                                                             <  4:490102/78‑1 (MQ=255)
                                                                                   tGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCAc                                                                >  3:578089/1‑139 (MQ=255)
                                                                                       gTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAg                                                                      >  1:1022220/1‑129 (MQ=255)
                                                                                        tttATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAgg                                                 >  4:735804/1‑149 (MQ=255)
                                                                                           aTTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATc                                                                             <  3:716351/118‑1 (MQ=255)
                                                                                           aTTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGa                                              >  1:297136/1‑149 (MQ=255)
                                                                                           aTTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGa                                              >  3:498065/1‑149 (MQ=255)
                                                                                            ttCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGaa                                                   >  3:1235501/1‑143 (MQ=255)
                                                                                            ttCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGc                                                >  3:1312100/1‑146 (MQ=255)
                                                                                            ttCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGc                                                <  4:1312100/146‑1 (MQ=255)
                                                                                             tCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTAc                                                                                                            <  1:577621/85‑1 (MQ=255)
                                                                                             tCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTAc                                                                                                            >  2:577621/1‑85 (MQ=255)
                                                                                             tCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCAc                                                            >  2:1187199/1‑133 (MQ=255)
                                                                                             tCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCAc                                                            <  1:1187199/133‑1 (MQ=255)
                                                                                               aGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTAt                                                                              >  1:555104/1‑113 (MQ=255)
                                                                                                gATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCg                                         >  1:1418509/1‑149 (MQ=255)
                                                                                                gATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCg                                         >  3:598399/1‑149 (MQ=255)
                                                                                                 aTAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGa                                        <  4:663143/149‑1 (MQ=255)
                                                                                                   aGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGata                                      >  3:29329/1‑149 (MQ=255)
                                                                                                    gTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGatat                                     >  2:548288/1‑149 (MQ=255)
                                                                                                    gTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGAACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatcatc                                                                                            <  4:698247/94‑1 (MQ=255)
                                                                                                     tCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatc                                                                                               >  4:207437/1‑90 (MQ=255)
                                                                                                     tCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAatc                                                                                               <  3:207437/90‑1 (MQ=255)
                                                                                                     tCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGAtata                                    >  2:1261806/1‑149 (MQ=255)
                                                                                                        tCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAg                                 >  2:1086045/1‑149 (MQ=255)
                                                                                                        tCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAg                                 <  4:29329/149‑1 (MQ=255)
                                                                                                            ggAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCAc                                                                >  1:1025856/1‑114 (MQ=255)
                                                                                                            ggAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCAc                                                                <  2:1025856/114‑1 (MQ=255)
                                                                                                              aaCAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGaa                                                   <  4:776601/125‑1 (MQ=255)
                                                                                                              aaCAAGATGGTGCCGCTAATCTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCa                                                                                          >  4:1038903/1‑86 (MQ=255)
                                                                                                              aaCAAGATGGTGCCGCTAATCTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCa                                                                                          <  3:1038903/86‑1 (MQ=255)
                                                                                                               aCAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCTACTTTATAGAAGGCGATACCg                                         >  1:1400440/1‑134 (MQ=255)
                                                                                                               aCAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCg                                         <  2:1400440/134‑1 (MQ=255)
                                                                                                               aCAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTc                          >  1:199025/1‑149 (MQ=255)
                                                                                                                cAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTcc                         <  2:1418509/149‑1 (MQ=255)
                                                                                                                  aGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCAc                                                            >  2:1445135/1‑112 (MQ=255)
                                                                                                                  aGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCAc                                                            <  1:1445135/112‑1 (MQ=255)
                                                                                                                   gATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGat                                       <  4:208453/132‑1 (MQ=255)
                                                                                                                   gATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGat                                       >  3:208453/1‑132 (MQ=255)
                                                                                                                   gATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCgg                             >  2:1175349/1‑142 (MQ=255)
                                                                                                                   gATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCgg                             <  1:1175349/142‑1 (MQ=255)
                                                                                                                    aTGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGt                     <  2:94960/149‑1 (MQ=255)
                                                                                                                       gTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAgg                                                 >  1:1354556/1‑118 (MQ=255)
                                                                                                                       gTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAgg                                                 <  2:1354556/118‑1 (MQ=255)
                                                                                                                       gTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCgg                  <  3:735804/149‑1 (MQ=255)
                                                                                                                         gCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGtt                <  1:1086045/149‑1 (MQ=255)
                                                                                                                         gCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGtt                <  2:502550/149‑1 (MQ=255)
                                                                                                                          ccGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGttt               <  4:498065/149‑1 (MQ=255)
                                                                                                                            gCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTaa             <  4:428271/149‑1 (MQ=255)
                                                                                                                                 tttCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACa        >  2:1077794/1‑149 (MQ=255)
                                                                                                                                       gCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGc                                                <  3:169988/103‑1 (MQ=255)
                                                                                                                                       gCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGc                                                >  4:169988/1‑103 (MQ=255)
                                                                                                                                       gCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGcc  <  1:1261806/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                   
GTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCGAGCTGCTTTTTCAGTTCCGGTACGCCGCCGTTAATCATCCAGTCTGGCTCTATCTTTGAAGGTTCACCCACTTTATAGAAGGCGATACCGATATACAGGCGGGTCCTGGTCGGTTTAACGACATCCGCC  >  NC_000913/1567467‑1567750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: