Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,626,126 C→T 5.5% D419N (GAT→AAT)  dcp ← dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,626,1260CT 5.5% 235.4 / 3.7 72D419N (GAT→AAT) dcpdipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base C (35/33);  new base T (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

TATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACA  >  NC_000913/1625983‑1626274
                                                                                                                                               |                                                                                                                                                    
tATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACggg                                                                                                                                                 <  2:963584/149‑1 (MQ=255)
   gACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCga                                                                                                                                              >  4:1323399/1‑149 (MQ=255)
             cAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACggg                                                                                                                                                 >  2:933258/1‑136 (MQ=255)
             cAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACggg                                                                                                                                                 <  1:933258/136‑1 (MQ=255)
              agagTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCa                                                                                                                                      <  4:384371/146‑1 (MQ=255)
              agagTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCa                                                                                                                                      >  3:384371/1‑146 (MQ=255)
                               tCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGt                                                                                                                                  <  2:298662/133‑1 (MQ=255)
                               tCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGt                                                                                                                                  >  1:298662/1‑133 (MQ=255)
                                     gCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGcc                                                                                                            >  3:566806/1‑149 (MQ=255)
                                        gCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCAtt                                                                                                         <  4:566806/149‑1 (MQ=255)
                                             tttCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGAt                                                                                                    >  3:429732/1‑149 (MQ=255)
                                                 tGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCaa                                                                                                 >  1:421377/1‑148 (MQ=255)
                                                 tGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCaa                                                                                                 <  2:421377/148‑1 (MQ=255)
                                                  gATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCAcc                                                                                                                                    >  1:688909/1‑112 (MQ=255)
                                                  gATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCAcc                                                                                                                                    <  2:688909/112‑1 (MQ=255)
                                                   aTAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCaaaaa                                                                                              <  4:429732/149‑1 (MQ=255)
                                                     aaTTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCCCCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAAtt                                                                                            >  3:1294651/1‑149 (MQ=255)
                                                        tGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTcc                                                                                         <  3:1323399/149‑1 (MQ=255)
                                                         gCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATg                                                                                                      <  2:970912/135‑1 (MQ=255)
                                                         gCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATg                                                                                                      >  1:970912/1‑135 (MQ=255)
                                                          cAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCGACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATc                                                                                                                                                      >  3:997394/1‑86 (MQ=255)
                                                          cAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATc                                                                                                                                                      <  4:997394/86‑1 (MQ=255)
                                                                          ccGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATaa                                                                                                                           >  1:929432/1‑97 (MQ=255)
                                                                          ccGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATaa                                                                                                                           <  2:929432/97‑1 (MQ=255)
                                                                               tgtgTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTa                                                                  <  4:1294651/149‑1 (MQ=255)
                                                                                   ttttATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGt                                                                   >  4:209432/1‑144 (MQ=255)
                                                                                   ttttATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGt                                                                   <  3:209432/144‑1 (MQ=255)
                                                                                   ttttATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACa                                                              >  2:217392/1‑149 (MQ=255)
                                                                                        ttaAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAat                                                         <  1:217392/149‑1 (MQ=255)
                                                                                            gCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAat                                                         >  2:55237/1‑145 (MQ=255)
                                                                                            gCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAat                                                         <  1:55237/145‑1 (MQ=255)
                                                                                              gTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGt                                                                   >  4:726926/1‑133 (MQ=255)
                                                                                              gTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGt                                                                   <  3:726926/133‑1 (MQ=255)
                                                                                               ttGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCaaaa                                                  >  3:179613/1‑149 (MQ=255)
                                                                                                  aTTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGaa                                                          >  3:367101/1‑138 (MQ=255)
                                                                                                  aTTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGaa                                                          <  4:367101/138‑1 (MQ=255)
                                                                                                   ttGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGtt                                              >  1:598444/1‑149 (MQ=255)
                                                                                                    tGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTc                                             <  4:179613/149‑1 (MQ=255)
                                                                                                      cTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGa                                           >  2:504735/1‑149 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCga                                                                                                                                              <  1:1397963/47‑1 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCga                                                                                                                                              >  2:1397963/1‑47 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGc                                                                                                                        <  3:552310/69‑1 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGc                                                                                                                        >  4:552310/1‑69 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTcc                                                                                         <  3:647077/100‑1 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTcc                                                                                         >  4:647077/1‑100 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATgg                                                                    >  1:970531/1‑121 (MQ=255)
                                                                                                         aacaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATgg                                                                    <  2:970531/121‑1 (MQ=255)
                                                                                                          acaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTa                                                                  >  1:716389/1‑122 (MQ=255)
                                                                                                          acaaAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTa                                                                  <  2:716389/122‑1 (MQ=255)
                                                                                                                      atccatGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGaa                           >  2:1208619/1‑149 (MQ=255)
                                                                                                                       tccatGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAag                          >  4:193893/1‑149 (MQ=255)
                                                                                                                        ccatGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAaga                         <  3:193893/149‑1 (MQ=255)
                                                                                                                          atGCACCGCCGCTTTTTGAATTACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGc                       >  1:255729/1‑149 (MQ=255)
                                                                                                                          atGCACCGCCGCTTTTTGAATTACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGc                       >  3:1345940/1‑149 (MQ=255)
                                                                                                                          atGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGc                       <  1:504735/149‑1 (MQ=255)
                                                                                                                            gCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAat                                                         >  1:201119/1‑113 (MQ=255)
                                                                                                                            gCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAat                                                         <  2:201119/113‑1 (MQ=255)
                                                                                                                                 gccgcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTccc                                                                                        <  2:709422/77‑1 (MQ=255)
                                                                                                                                 gccgcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTccc                                                                                        >  1:709422/1‑77 (MQ=255)
                                                                                                                                    gcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTcc                                                                                         >  3:462297/1‑73 (MQ=255)
                                                                                                                                    gcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTcc                                                                                         <  4:462297/73‑1 (MQ=255)
                                                                                                                                     cTTTTTGAATTACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGt            <  2:255729/149‑1 (MQ=255)
                                                                                                                                     cTTTTTGAATTACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGt            <  4:1345940/149‑1 (MQ=255)
                                                                                                                                      tttttGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCAtt                                                                                                         <  3:862552/55‑1 (MQ=255)
                                                                                                                                      tttttGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCAtt                                                                                                         >  4:862552/1‑55 (MQ=255)
                                                                                                                                      tttttGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc           >  4:315712/1‑149 (MQ=255)
                                                                                                                                            aaTCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCaaaa                                                  <  1:380491/104‑1 (MQ=255)
                                                                                                                                            aaTCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCaaaa                                                  >  2:380491/1‑104 (MQ=255)
                                                                                                                                            aaTCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACtt                                    >  4:118727/1‑118 (MQ=255)
                                                                                                                                            aaTCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACtt                                    <  3:118727/118‑1 (MQ=255)
                                                                                                                                            aaTCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGaa      <  4:95058/148‑1 (MQ=255)
                                                                                                                                            aaTCACGGGCGAAGAAATCACCGTAAAAGAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGaa      >  3:95058/1‑148 (MQ=255)
                                                                                                                                              tCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATa    <  1:588902/148‑1 (MQ=255)
                                                                                                                                              tCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATa    >  2:588902/1‑148 (MQ=255)
                                                                                                                                               cACGGGCGAAGCAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATaca  <  1:1208619/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACA  >  NC_000913/1625983‑1626274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑