| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,681,622 | T→A | 8.7% | L216Q (CTG→CAG) | folM → | dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,681,622 | 0 | T | A | 8.7% | 347.7 / 15.4 | 101 | L216Q (CTG→CAG) | folM | dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base T (46/46); new base A (5/4); total (51/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
AGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCG > NC_000913/1681481‑1681770 | aGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAt < 2:485324/149‑1 (MQ=255) cTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa > 4:11532/1‑146 (MQ=255) cTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa < 3:11532/146‑1 (MQ=255) cACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCGAtcacttc < 4:575496/149‑2 (MQ=255) aCCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac > 4:1148423/1‑149 (MQ=255) agtgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCa < 3:1148423/149‑1 (MQ=255) tgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttactt < 1:626781/139‑1 (MQ=255) tgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttactt > 2:626781/1‑139 (MQ=255) gtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTCACCAGTTGCtt < 3:963566/149‑1 (MQ=255) tgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCttt < 1:839126/149‑1 (MQ=255) tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCg > 3:334646/1‑149 (MQ=255) tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCg > 2:1395517/1‑149 (MQ=255) ccATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg < 1:1205584/109‑1 (MQ=255) ccATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg > 2:1205584/1‑109 (MQ=255) cGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTc < 1:1395517/149‑1 (MQ=255) cTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTccc > 4:522969/1‑149 (MQ=255) tGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa < 3:1405500/106‑1 (MQ=255) tGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa > 4:1405500/1‑106 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg < 4:379033/100‑1 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg > 3:379033/1‑100 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACt > 1:525049/1‑149 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACt < 3:739829/149‑1 (MQ=255) gTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGt > 2:193723/1‑123 (MQ=255) gTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGt < 1:193723/123‑1 (MQ=255) aaTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCgg > 4:247081/1‑149 (MQ=255) gAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcg > 1:1383790/1‑149 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgt > 3:1077905/1‑149 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttacttac > 4:335004/1‑102 (MQ=255) aCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgtc < 3:522969/149‑1 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGc > 2:172244/1‑138 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGc < 1:172244/138‑1 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCt < 4:334646/149‑1 (MQ=255) tgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGc < 4:1077905/149‑1 (MQ=255) tgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGc < 3:247081/149‑1 (MQ=255) tgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGtt > 3:1318387/1‑149 (MQ=255) cGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAAt < 2:525049/149‑1 (MQ=255) tatCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAg > 3:1099108/1‑149 (MQ=255) atCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCa < 3:352527/87‑1 (MQ=255) atCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCa > 4:352527/1‑87 (MQ=255) tCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAAt < 4:260100/143‑1 (MQ=255) tCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAAt > 3:260100/1‑143 (MQ=255) acaacaGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtc < 1:579268/125‑1 (MQ=255) acaacaGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtc > 2:579268/1‑125 (MQ=255) tGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGAt < 2:1418774/107‑1 (MQ=255) tGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGAt > 1:1418774/1‑107 (MQ=255) aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACtt < 1:858042/102‑1 (MQ=255) aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACtt > 2:858042/1‑102 (MQ=255) aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCt < 4:514192/119‑1 (MQ=255) aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCt > 3:514192/1‑119 (MQ=255) aTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATgg > 4:1296198/1‑106 (MQ=255) aTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATgg < 3:1296198/106‑1 (MQ=255) aTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc < 2:325337/149‑1 (MQ=255) aaaTCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCaa < 4:1099108/149‑1 (MQ=255) aaTCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtc < 2:579743/108‑1 (MQ=255) aaTCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtc > 1:579743/1‑108 (MQ=255) aTCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAaca > 2:851596/1‑149 (MQ=255) cACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCAt > 1:1276533/1‑110 (MQ=255) cACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCAt < 2:1276533/110‑1 (MQ=255) aCTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACTAGTTGCTTTGTCAcc > 1:1419826/1‑76 (MQ=255) aCTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACTAGTTGCTTTGTCAcc < 2:1419826/76‑1 (MQ=255) tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtc < 3:58301/102‑1 (MQ=255) tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtc > 4:58301/1‑102 (MQ=255) tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATc < 1:398658/128‑1 (MQ=255) tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc > 2:728756/1‑140 (MQ=255) tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc < 1:728756/140‑1 (MQ=255) aaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTa < 2:1383790/149‑1 (MQ=255) aaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTa > 1:614323/1‑149 (MQ=255) aCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATgta < 2:1158984/112‑3 (MQ=255) aCCGCGCCTGGCGAGAAAGAAGTGATCGAACTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCa > 1:1158984/1‑112 (MQ=255) gcgcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATccc > 4:1141209/1‑149 (MQ=255) gcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAg < 1:1112141/108‑1 (MQ=255) ggCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAaca < 2:614323/149‑1 (MQ=255) gagaAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGc > 2:511989/1‑149 (MQ=255) agaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCa > 1:594509/1‑109 (MQ=255) agaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCa < 2:594509/109‑1 (MQ=255) gaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCaa > 4:411908/1‑149 (MQ=255) aaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAAc > 3:764555/1‑149 (MQ=255) agtaagTAATCGACCAGGTCGATCACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCcaca < 4:603542/139‑1 (MQ=255) agtaagTAATCGACCAGGTCGATCACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAaca > 3:603542/4‑142 (MQ=255) aagaagTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACa > 1:118273/1‑149 (MQ=255) agaagTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAg > 2:459008/1‑149 (MQ=255) aagTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGaa < 3:1141209/149‑1 (MQ=255) agtaaTCGACCAGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATccccc < 4:262557/130‑1 (MQ=255) agTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAAc < 1:511989/149‑1 (MQ=255) agTAATCGACCAGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATccccc < 4:857076/134‑1 (MQ=255) agTAATCGACCAGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATccccc > 3:857076/1‑134 (MQ=255) agTAATCGACCAGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATccccc > 3:262557/1‑134 (MQ=255) agTAATCGACCAGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAAc > 4:1448590/1‑149 (MQ=255) gtaaTCGACCAGGTCGATCACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAAcc > 4:499638/4‑149 (MQ=255) gATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACcaa < 2:118273/149‑1 (MQ=255) aTCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACcaac > 3:1215303/1‑149 (MQ=255) aCCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCa > 3:338434/1‑97 (MQ=255) aCCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACaaaa < 1:459008/149‑1 (MQ=255) cTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAgc < 4:764555/149‑1 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTaa < 1:1408047/77‑1 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTaa > 2:1408047/1‑77 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc < 4:341968/101‑1 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc > 3:341968/1‑101 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACcaa > 2:232845/1‑141 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAgcg > 1:1070225/1‑149 (MQ=255) | AGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCG > NC_000913/1681481‑1681770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |