Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,701,108 A→T 7.7% N51I (AAT→ATT)  malY → PLP‑dependent beta‑cystathionase and maltose regulon regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,701,1080AT 7.7% 319.5 / 14.5 91N51I (AAT→ATT) malYPLP‑dependent beta‑cystathionase and maltose regulon regulator
Reads supporting (aligned to +/- strand):  ref base A (40/44);  new base T (4/3);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01

TTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTT  >  NC_000913/1700960‑1701256
                                                                                                                                                    |                                                                                                                                                    
ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa                                                                                                                                                      <  2:540169/149‑1 (MQ=255)
ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGaa                                                                                                                                                      >  1:1390116/1‑149 (MQ=255)
   gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCa                                                                                                                                                   <  2:1390116/149‑1 (MQ=255)
     tttttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCTGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAgc                                                                                                                                                 <  3:1214233/149‑1 (MQ=255)
     tttttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCTGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAgc                                                                                                                                                 >  4:1214233/1‑149 (MQ=255)
     tttttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAgc                                                                                                                                                 <  2:532565/149‑1 (MQ=255)
     tttttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAgc                                                                                                                                                 <  2:1443587/149‑1 (MQ=255)
        ttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCAGCGcc                                                                                                                                              <  2:47012/149‑1 (MQ=255)
         tCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCt                                                                                                                                             <  4:1233239/149‑1 (MQ=255)
           aaaGGTCTTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACTATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGa                                                                                                                                           >  2:1089124/1‑149 (MQ=255)
           aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGa                                                                                                                                           <  4:450748/149‑1 (MQ=255)
           aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCACCGCCTGa                                                                                                                                           >  3:450748/1‑149 (MQ=255)
               gTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCa                                                                                                                                       >  4:115280/1‑149 (MQ=255)
                     gATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGt                                                                                                                                 >  2:1333547/1‑149 (MQ=255)
                     gATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGt                                                                                                                                 >  2:268440/1‑149 (MQ=255)
                      aTCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTa                                                                                                                                >  1:816532/1‑149 (MQ=255)
                      aTCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTa                                                                                                                                >  1:1017014/1‑149 (MQ=255)
                        cGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTAtt                                                                                                                              <  1:1089124/149‑1 (MQ=255)
                          tCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGc                                                                                                                                   <  2:129810/142‑1 (MQ=255)
                          tCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGc                                                                                                                                   >  1:129810/1‑142 (MQ=255)
                              ggCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTa                                                                                                                        >  2:986512/1‑149 (MQ=255)
                                 acaTGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAg                                                                                                                     >  3:482946/1‑149 (MQ=255)
                                     ggTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGc                                                                                                                 <  1:1333547/149‑1 (MQ=255)
                                     ggTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGc                                                                                                                 <  2:816532/149‑1 (MQ=255)
                                     ggTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGc                                                                                                                 <  2:773149/149‑1 (MQ=255)
                                      gtgtACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                                                                                                     >  3:648255/1‑128 (MQ=255)
                                      gtgtACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                                                                                                     <  4:648255/128‑1 (MQ=255)
                                           cacaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaaa                                                                                                           >  3:865330/1‑149 (MQ=255)
                                            acaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAAt                                                                                                                                                     <  1:953055/106‑1 (MQ=255)
                                            acaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAAt                                                                                                                                                     >  2:953055/1‑106 (MQ=255)
                                            acaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCa                                                                                                                                                   <  3:1077737/108‑1 (MQ=255)
                                            acaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCa                                                                                                                                                   >  4:1077737/1‑108 (MQ=255)
                                             caGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaaaaa                                                                                                         <  1:268440/149‑1 (MQ=255)
                                             caGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGATCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaacaa                                                                                                         <  4:482946/149‑1 (MQ=255)
                                              aGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAAc                                                                                                        <  4:865330/149‑1 (MQ=255)
                                                 gggATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGAt                                                                                                     <  4:1117520/149‑1 (MQ=255)
                                                     ttATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGt                                                                                                 <  3:519269/149‑1 (MQ=255)
                                                         gTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct                                                                                             <  1:986512/149‑1 (MQ=255)
                                                              tGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCgg                                                                                        >  3:534922/1‑149 (MQ=255)
                                                                   gTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTAtt                                                                                   >  2:1376139/1‑149 (MQ=255)
                                                                           aCTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTac                                                                                    >  1:435152/1‑139 (MQ=255)
                                                                           aCTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTAt                                                                                    <  2:435152/140‑1 (MQ=255)
                                                                              gctgACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGtt                                                                        <  4:534922/149‑1 (MQ=255)
                                                                               ctgACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTg                                                                                                                            >  2:695048/1‑96 (MQ=255)
                                                                               ctgACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTg                                                                                                                            <  1:695048/96‑1 (MQ=255)
                                                                                 gACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTc                                                                     <  1:1376139/149‑1 (MQ=255)
                                                                                   ccTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtctc                                                                                            >  4:896170/1‑124 (MQ=255)
                                                                                   ccTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtctc                                                                                            <  3:896170/124‑1 (MQ=255)
                                                                                   ccTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCa                                                                   <  3:115280/149‑1 (MQ=255)
                                                                                     tGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGGACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCACGGCTATTGCCCACTGGTTTTcagat                                                                 >  1:1051209/1‑145 (MQ=255)
                                                                                             ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTa                                                         >  2:527708/1‑149 (MQ=255)
                                                                                             ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTa                                                         <  2:1017014/149‑1 (MQ=255)
                                                                                              tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTac                                                        <  1:527708/149‑1 (MQ=255)
                                                                                                 cGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACAcc                                                     >  2:791852/1‑149 (MQ=255)
                                                                                                        aGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCg                                              >  1:266445/1‑149 (MQ=255)
                                                                                                         gACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGAt                                                                                                     >  4:448764/1‑93 (MQ=255)
                                                                                                         gACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGAt                                                                                                     <  3:448764/93‑1 (MQ=255)
                                                                                                          aCATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGAt                                                                                                     >  4:136058/1‑92 (MQ=255)
                                                                                                          aCATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGAt                                                                                                     <  3:136058/92‑1 (MQ=255)
                                                                                                            aTGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCAGCGCCt                                                                                                                                             >  1:1398533/1‑50 (MQ=255)
                                                                                                            aTGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGATTCAGCGCCt                                                                                                                                             <  2:1398533/50‑1 (MQ=255)
                                                                                                            aTGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTcg                                                                                           >  3:791373/1‑100 (MQ=255)
                                                                                                            aTGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTcg                                                                                           <  4:791373/100‑1 (MQ=255)
                                                                                                                 ttttGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCAt                                                           <  1:1040525/127‑1 (MQ=255)
                                                                                                                 ttttGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCAt                                                           >  2:1040525/1‑127 (MQ=255)
                                                                                                                      ccACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACggtg                                >  1:1236694/1‑149 (MQ=255)
                                                                                                                             ccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATg                         <  1:791852/149‑1 (MQ=255)
                                                                                                                               ccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATtctc                                        <  1:418035/132‑1 (MQ=255)
                                                                                                                               ccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATtctc                                        >  2:418035/1‑132 (MQ=255)
                                                                                                                                 tGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGGAAAACGATGAGTTtct                                                                                             >  2:976830/1‑77 (MQ=255)
                                                                                                                                 tGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct                                                                                             <  1:976830/77‑1 (MQ=255)
                                                                                                                                   cATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCAt                                                >  3:357865/1‑120 (MQ=255)
                                                                                                                                   cATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCAt                                                <  4:357865/120‑1 (MQ=255)
                                                                                                                                    aTTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                     <  1:762435/130‑1 (MQ=255)
                                                                                                                                    aTTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                     >  2:762435/1‑130 (MQ=255)
                                                                                                                                     ttATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGAc                                    >  1:642996/1‑130 (MQ=255)
                                                                                                                                     ttATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGAc                                    <  2:642996/130‑1 (MQ=255)
                                                                                                                                        tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGttt                                                                       <  3:341080/92‑1 (MQ=255)
                                                                                                                                        tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGttt                                                                       >  4:341080/1‑92 (MQ=255)
                                                                                                                                        atcaGGCGCTGATTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTc              >  1:442434/4‑149 (MQ=255)
                                                                                                                                         tcaGGCGCTGATTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                           <  4:1166010/133‑1 (MQ=255)
                                                                                                                                         tcaGGCGCTGATTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                           >  3:1166010/3‑135 (MQ=255)
                                                                                                                                          gAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCAt            <  2:266445/149‑1 (MQ=255)
                                                                                                                                             gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATg                         >  3:601771/1‑133 (MQ=255)
                                                                                                                                             gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATg                         <  4:601771/133‑1 (MQ=255)
                                                                                                                                             gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCctct                 <  1:1179264/141‑1 (MQ=255)
                                                                                                                                             gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCt                 >  2:1179264/1‑141 (MQ=255)
                                                                                                                                               gcTGATTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCtata       >  1:1286489/1‑149 (MQ=255)
                                                                                                                                               gcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCtata       >  2:1005510/1‑149 (MQ=255)
                                                                                                                                               gcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCtata       <  3:656673/149‑1 (MQ=255)
                                                                                                                                               gcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCtata       >  4:656673/1‑149 (MQ=255)
                                                                                                                                                 tGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTatat      <  4:1192503/148‑1 (MQ=255)
                                                                                                                                                 tGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTatat      >  3:1192503/1‑148 (MQ=255)
                                                                                                                                                    aTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGtt  >  3:1338879/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTT  >  NC_000913/1700960‑1701256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: