Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,721,515 G→T 7.6% E84D (GAG→GAT ydhJ → putative membrane fusion protein (MFP) of YdhJK efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,721,5150GT 7.6% 273.6 / 11.3 79E84D (GAG→GATydhJputative membrane fusion protein (MFP) of YdhJK efflux pump
Reads supporting (aligned to +/- strand):  ref base G (37/36);  new base T (3/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

ACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCAATGTTAAAGCGATGCAGGCCA  >  NC_000913/1721373‑1721663
                                                                                                                                              |                                                                                                                                                    
aCGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAAc                                                                                                                                                <  3:714247/149‑1 (MQ=255)
   ggAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGCTCGGCGATCTGaaa                                                                                                                                                >  4:957940/1‑145 (MQ=255)
   ggAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGCTCGGCGATCTGaaa                                                                                                                                                <  3:957940/146‑2 (MQ=255)
    gAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCATCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGcac                                                                                                                                            >  4:431188/1‑149 (MQ=255)
     aaaaTACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGcaca                                                                                                                                           >  4:226875/1‑149 (MQ=255)
                 gAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGc                                                                                                                               <  3:431188/149‑1 (MQ=255)
                       gTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAAc                                                                                                                                                >  3:953824/1‑126 (MQ=255)
                       gTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAAc                                                                                                                                                <  4:953824/126‑1 (MQ=255)
                       gTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGcac                                                                                                                                            >  1:826810/1‑130 (MQ=255)
                       gTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGcac                                                                                                                                            <  2:826810/130‑1 (MQ=255)
                       gTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAgc                                                                                                                         <  1:582360/149‑1 (MQ=255)
                       gTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAgc                                                                                                                         >  2:582360/1‑149 (MQ=255)
                        tttCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGCTCGGCGATCTGaaa                                                                                                                                                <  4:612947/125‑2 (MQ=255)
                        tttCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGCTCGGCGATCTGaaa                                                                                                                                                >  3:612947/1‑124 (MQ=255)
                            tATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAgcgc                                                                                                                       <  2:1449988/146‑1 (MQ=255)
                            tATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAgcgc                                                                                                                       >  1:1449988/1‑146 (MQ=255)
                            tATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAAt                                                                                                                    >  4:529317/1‑149 (MQ=255)
                             aTCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATc                                                                                                                   >  1:697246/1‑149 (MQ=255)
                                 ccccccAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTgtc                                                                                                               <  3:226875/149‑3 (MQ=255)
                                   ccACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCt                                                                                                             >  2:47730/1‑149 (MQ=255)
                                                gACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAAt                                                                                                >  3:38027/1‑149 (MQ=255)
                                                gACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAAt                                                                                                <  2:936112/149‑1 (MQ=255)
                                                gACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAAt                                                                                                >  1:936112/1‑149 (MQ=255)
                                                          tgAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGa                                                                                      <  3:529317/149‑1 (MQ=255)
                                                              cTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCCCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtcg                                                                                  >  2:848180/1‑149 (MQ=255)
                                                                gAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtcgtc                                                                                <  1:848180/149‑1 (MQ=255)
                                                                     taAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAg                                                                                                                                          <  1:236192/86‑1 (MQ=255)
                                                                     taAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAg                                                                                                                                          >  2:236192/1‑86 (MQ=255)
                                                                     taAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtcgtc                                                                                <  1:273657/144‑1 (MQ=255)
                                                                     taAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtcgtc                                                                                >  2:273657/1‑144 (MQ=255)
                                                                     taAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATcgtcgtcgt                                                                           <  2:697246/149‑1 (MQ=255)
                                                                       aaaGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCa                                                                         >  2:608615/1‑149 (MQ=255)
                                                                        aaGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGAc                                                                                                               <  3:1451367/110‑1 (MQ=255)
                                                                        aaGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGAc                                                                                                               >  4:1451367/1‑110 (MQ=255)
                                                                        aaGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCAt                                                                        >  1:1189906/1‑149 (MQ=255)
                                                                         aGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtcgtcgtc                                                                             >  2:1299271/1‑143 (MQ=255)
                                                                         aGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtcgtcgtc                                                                             <  1:1299271/143‑1 (MQ=255)
                                                                           aCAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCAtctc                                                                     >  3:360298/1‑149 (MQ=255)
                                                                              aCCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTca                                                                  <  1:608615/149‑1 (MQ=255)
                                                                                cAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAAAGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGa                                                                                                                >  4:2528/1‑101 (MQ=255)
                                                                                cAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAAAGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGa                                                                                                                <  3:2528/101‑1 (MQ=255)
                                                                                 aGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCaa                                                                                                                     <  1:972211/95‑1 (MQ=255)
                                                                                 aGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCaa                                                                                                                     >  2:972211/1‑95 (MQ=255)
                                                                                  gCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACaaa                                                              >  2:1064710/1‑149 (MQ=255)
                                                                                  gCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCGTTCAGCTCGGCGATCTGaaagg                                                                                                                                              <  1:841277/69‑4 (MQ=25)
                                                                                  gCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCGTTCAGCTCGGCGATCTGaaagg                                                                                                                                              >  2:841277/1‑66 (MQ=25)
                                                                                   cTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACaaaa                                                             <  2:1189906/149‑1 (MQ=255)
                                                                                   cTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACaaaa                                                             <  1:47730/149‑1 (MQ=255)
                                                                                       ttAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTc                                                                                                                                      >  3:176647/1‑72 (MQ=255)
                                                                                       ttAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTc                                                                                                                                      <  4:176647/72‑1 (MQ=255)
                                                                                         aaCGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTAt                                                       <  1:1064710/149‑1 (MQ=255)
                                                                                                  gATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGcc                                                <  1:1146411/147‑1 (MQ=255)
                                                                                                  gATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGcc                                                <  1:93437/147‑1 (MQ=255)
                                                                                                  gATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGcc                                                >  2:93437/1‑147 (MQ=255)
                                                                                                  gATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGcc                                                >  2:1146411/1‑147 (MQ=255)
                                                                                                    tcttctGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtc                                                                                   <  1:36/110‑1 (MQ=255)
                                                                                                    tcttctGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAAtc                                                                                   >  2:36/1‑110 (MQ=255)
                                                                                                     cttctGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGa                                                                                                                >  3:129911/1‑80 (MQ=255)
                                                                                                     cttctGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGa                                                                                                                <  4:129911/80‑1 (MQ=255)
                                                                                                         tGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTc                                       <  4:360298/149‑1 (MQ=255)
                                                                                                           cTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGa                                     >  3:1202746/1‑149 (MQ=255)
                                                                                                           cTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGa                                     <  4:1202746/149‑1 (MQ=255)
                                                                                                            tCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATcgtcgtcgt                                                                           >  2:800767/1‑110 (MQ=255)
                                                                                                            tCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATcgtcgtcgt                                                                           <  1:800767/110‑1 (MQ=255)
                                                                                                                 atcgatAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCg                                                                                       <  1:293200/93‑1 (MQ=255)
                                                                                                                 atcgatAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCg                                                                                       >  2:293200/1‑93 (MQ=255)
                                                                                                                    gatAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATAc                                  >  4:1097980/1‑143 (MQ=255)
                                                                                                                    gatAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATAc                                  <  3:1097980/143‑1 (MQ=255)
                                                                                                                     atAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAAc                           >  4:401622/1‑149 (MQ=255)
                                                                                                                      tAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACt                                        <  2:1272647/135‑1 (MQ=255)
                                                                                                                      tAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACt                                        >  1:1272647/1‑135 (MQ=255)
                                                                                                                        aaaCGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGa                                     <  2:989562/136‑1 (MQ=255)
                                                                                                                        aaaCGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGa                                     >  1:989562/1‑136 (MQ=255)
                                                                                                                          aCGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCaa                      >  4:557104/1‑149 (MQ=255)
                                                                                                                               cTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCAATGTTa                 >  3:868362/1‑149 (MQ=255)
                                                                                                                                tttCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTa                                                        <  2:1195502/109‑1 (MQ=255)
                                                                                                                                tttCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTa                                                        >  1:1195502/1‑109 (MQ=255)
                                                                                                                                 ttCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCAATGTTaaa               <  3:401622/149‑1 (MQ=255)
                                                                                                                                      aTCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCAATGTTAAAGCGAt          <  4:868362/149‑1 (MQ=255)
                                                                                                                                              gCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCAATGTTAAAGCGATGCAGGCCa  >  2:82773/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
ACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTTTCAGATCGCCGAGCTGAACGCACAGGCTCAGCTGGCGAAAGCGCAATCTGACCTCGCAAAAGCCAATAACGAAGCGAATCGTCGTCGTCATCTCTCACAAAATTTTATTTCTGCCGAAGAACTCGATACCGCTAACCTCAATGTTAAAGCGATGCAGGCCA  >  NC_000913/1721373‑1721663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: