Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,749,025 C→T 5.5% A179T (GCC→ACC)  ydhT ← FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,749,0250CT 5.5% 425.3 / 10.4 126A179T (GCC→ACC) ydhTFNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand):  ref base C (60/59);  new base T (4/3);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 126 total aligned reads displayed.

CTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGACTGTT  >  NC_000913/1748878‑1749172
                                                                                                                                                   |                                                                                                                                                   
ctGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCg                                                                                                                                                    >  2:521630/1‑149 (MQ=255)
  ggCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGAc                                                                                                                                                  >  2:983308/1‑149 (MQ=255)
      cGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGa                                                                                                                                              <  1:983308/149‑1 (MQ=255)
          aataatTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACAcc                                                                                                                                          >  3:947481/1‑149 (MQ=255)
               ttGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCa                                                                                                                                     <  4:947481/149‑1 (MQ=255)
                    tCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCaa                                                                                                                                <  4:1179791/149‑1 (MQ=255)
                    tCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCaa                                                                                                                                <  4:1097594/149‑1 (MQ=255)
                        ccACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTa                                                                                                                            <  4:878462/149‑1 (MQ=255)
                         cACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCTAATGTAc                                                                                                                           >  3:937493/1‑149 (MQ=255)
                              ccGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTa                                                                                                                            <  4:1278709/143‑1 (MQ=255)
                              ccGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTa                                                                                                                            >  3:1278709/1‑143 (MQ=255)
                                    tCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGa                                                                                                                                                   >  1:1209015/1‑114 (MQ=255)
                                      aCTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGa                                                                                                                                              >  1:780971/1‑117 (MQ=255)
                                      aCTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTa                                                                                                                            <  1:1063321/135‑1 (MQ=255)
                                      aCTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTa                                                                                                                            >  2:1063321/1‑135 (MQ=255)
                                        tACCGTTTAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCa                                                                                                            >  2:801346/1‑149 (MQ=255)
                                        tACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCa                                                                                                            >  3:323716/1‑149 (MQ=255)
                                            gTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGtt                                                                                                                                       >  3:774412/1‑118 (MQ=255)
                                            gTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGtt                                                                                                                                       <  4:774412/118‑1 (MQ=255)
                                             ttGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCaa                                                                                                                                <  1:20725/124‑1 (MQ=255)
                                             ttGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCaa                                                                                                                                >  2:20725/1‑124 (MQ=255)
                                             ttGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCg                                                                                                       >  1:1060053/1‑149 (MQ=255)
                                                aaTTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTa                                                                                                    >  2:46630/1‑149 (MQ=255)
                                                 aTTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTa                                                                                                    <  4:251003/148‑1 (MQ=255)
                                                  ttttCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTc                                                                                                               <  3:1175309/136‑1 (MQ=255)
                                                  ttttCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTc                                                                                                               >  4:1175309/1‑136 (MQ=255)
                                                  ttttCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTAtt                                                                                                  >  3:779955/1‑149 (MQ=255)
                                                   tttCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACa                                                                                                                                                 <  1:1372255/101‑1 (MQ=255)
                                                   tttCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACa                                                                                                                                                 >  2:1372255/1‑101 (MQ=255)
                                                   tttCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTc                                                                                                 <  2:1060053/149‑1 (MQ=255)
                                                       aCCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCaa                                                                                             >  4:632825/1‑149 (MQ=255)
                                                       aCCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCaa                                                                                             <  1:46630/149‑1 (MQ=255)
                                                         cGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATc                                                                                           <  4:323716/149‑1 (MQ=255)
                                                            tGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGa                                                                                        >  4:364603/1‑149 (MQ=255)
                                                              gCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACt                                                                                      >  2:1186094/1‑149 (MQ=255)
                                                                aaTGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGc                                                                                    >  4:556895/1‑149 (MQ=255)
                                                                 atgatgTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCt                                                                                   >  2:1171062/1‑149 (MQ=255)
                                                                      gtgtACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTAtctc                                                                              >  3:633090/1‑149 (MQ=255)
                                                                       tgtACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACAt                                                                                                                                                >  1:625472/1‑82 (MQ=255)
                                                                       tgtACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACAt                                                                                                                                                <  2:625472/82‑1 (MQ=255)
                                                                         tACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAg                                                                           >  4:548023/1‑149 (MQ=255)
                                                                           cATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAgcg                                                                         >  3:440439/1‑149 (MQ=255)
                                                                             ttGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgct                                                                       >  1:619645/1‑149 (MQ=255)
                                                                               gTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctgc                                                                     <  3:364603/149‑1 (MQ=255)
                                                                               gTCGCCGCTGTTGGCAATTCTGGCGTTTACGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctgc                                                                     <  4:937493/149‑1 (MQ=255)
                                                                                tCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctgct                                                                    <  1:1186094/149‑1 (MQ=255)
                                                                                     gcTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTc                                                                                                 <  4:358626/115‑1 (MQ=255)
                                                                                     gcTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATcc                                                               <  3:667032/149‑1 (MQ=255)
                                                                                         ttGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctg                                                                      >  3:608621/1‑138 (MQ=255)
                                                                                         ttGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctg                                                                      <  4:608621/138‑1 (MQ=255)
                                                                                          tGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCgaa                                                          <  4:779955/149‑1 (MQ=255)
                                                                                            acAATTCTGGCGTTTGGGCCAAAGCTGGCGATTTGGAGCAAGGCACCATTACTGGCGACATGACACCGTTCACCAAAAATACAAACGCGTACTCGCATTCCAGTATTCGCTATTCTACTGCTAGCTACTGCGCTTCTTATCCGCgaaga                                                        >  2:1221929/2‑149 (MQ=255)
                                                                                              aaTTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTa                                                                                                    <  2:545900/103‑1 (MQ=255)
                                                                                              aaTTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTa                                                                                                    >  1:545900/1‑103 (MQ=255)
                                                                                              aaTTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAAt                                                      <  1:1171062/149‑1 (MQ=255)
                                                                                                ttCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTa                                                    >  4:259592/1‑149 (MQ=255)
                                                                                                 tCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTAc                                                   >  3:1148896/1‑149 (MQ=255)
                                                                                                 tCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTAc                                                   <  1:801346/149‑1 (MQ=255)
                                                                                                  cTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTAcc                                                  <  4:440439/149‑1 (MQ=255)
                                                                                                   tGGCGTTTGCGCCACAGCTGGCGATTTGCACCAGCTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCCTTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCg                                                 >  1:469955/1‑149 (MQ=255)
                                                                                                   tGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctgc                                                                     <  3:782536/129‑1 (MQ=255)
                                                                                                   tGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCgctgc                                                                     >  4:782536/1‑129 (MQ=255)
                                                                                                   tGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCg                                                 >  1:1248909/1‑149 (MQ=255)
                                                                                                   tGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCg                                                 <  2:951360/149‑1 (MQ=255)
                                                                                                      cGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGtt                                              <  2:570764/149‑1 (MQ=255)
                                                                                                       gTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTa                                             <  1:1056450/149‑1 (MQ=255)
                                                                                                             gcCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAg                                          <  2:16970/146‑1 (MQ=255)
                                                                                                             gcCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCaa                                       >  1:400203/1‑149 (MQ=255)
                                                                                                             gcCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCaa                                       >  1:1325383/1‑149 (MQ=255)
                                                                                                              ccAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAAc                                      <  1:521630/149‑1 (MQ=255)
                                                                                                              ccAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAAc                                      <  3:632825/149‑1 (MQ=255)
                                                                                                              ccAAAGCTGGCGATTTGCACCAGGACACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAAc                                      <  2:469955/149‑1 (MQ=255)
                                                                                                                     tGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAGCGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCTCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTgcg                               >  1:1190491/1‑149 (MQ=255)
                                                                                                                     tGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTgcg                               >  1:536286/1‑149 (MQ=255)
                                                                                                                       gCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGcgcg                             <  3:556895/149‑1 (MQ=255)
                                                                                                                           tttGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCa                         >  2:670418/1‑149 (MQ=255)
                                                                                                                             tGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCAcc                       <  3:259592/149‑1 (MQ=255)
                                                                                                                                aCCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACaaa                                                                                                                        >  2:660643/1‑49 (MQ=255)
                                                                                                                                aCCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACaaa                                                                                                                        <  1:660643/49‑1 (MQ=255)
                                                                                                                                aCCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGa                    >  1:1056178/1‑149 (MQ=255)
                                                                                                                                 tcatgTCGCCAGTAATGGTGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAg                   >  2:1069471/5‑149 (MQ=255)
                                                                                                                                  cAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCgaa                                                          >  1:366592/1‑109 (MQ=255)
                                                                                                                                  cAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCgaa                                                          <  2:366592/109‑1 (MQ=255)
                                                                                                                                   atgTCGCCAGTAATGGTGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCgaag                                                         >  3:1186472/3‑109 (MQ=255)
                                                                                                                                   atgTCGCCAGTAATGGTGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCgaag                                                         <  4:1186472/107‑1 (MQ=255)
                                                                                                                                   atgTCGCCAGTAATGGTGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCt                 >  4:244861/3‑149 (MQ=255)
                                                                                                                                     gTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGc               >  2:110590/1‑149 (MQ=255)
                                                                                                                                         ccATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCt                                                                                   <  2:886836/77‑1 (MQ=255)
                                                                                                                                         ccATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAAt           <  1:670418/149‑1 (MQ=255)
                                                                                                                                         ccATTACTGGCGACATGACACCATTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCt                                                                                   >  1:886836/1‑77 (MQ=255)
                                                                                                                                             taatGGTGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGa       <  1:1370331/146‑1 (MQ=255)
                                                                                                                                             tACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGa       <  2:400203/149‑1 (MQ=255)
                                                                                                                                             tACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGa       <  3:454903/149‑1 (MQ=255)
                                                                                                                                             tACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGa       <  2:1190491/149‑1 (MQ=255)
                                                                                                                                                tGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAt                                                                                            >  3:291917/1‑61 (MQ=255)
                                                                                                                                                tGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGa                                                                                        <  3:771816/65‑1 (MQ=255)
                                                                                                                                                tGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGa                                                                                        >  4:771816/1‑65 (MQ=255)
                                                                                                                                                tGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGc               <  4:278903/138‑1 (MQ=255)
                                                                                                                                                tGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGc               >  3:278903/1‑138 (MQ=255)
                                                                                                                                                  gCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGACTGtt  >  2:318926/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCGACTGCTATCTCCAGCGCTGCTTATCCGCGAAGAATTACCGGTTACAGCAACCCGTGCGCGTTCACCGGAGCTGCCAATGTGACTGTT  >  NC_000913/1748878‑1749172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: