| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,764,695 | A→G | 10.5% | intergenic (‑309/+18) | sufA ← / ← rydB | Fe‑S cluster assembly protein/novel sRNA, function unknown |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,764,695 | 0 | A | G | 10.5% | 168.3 / 9.5 | 58 | intergenic (‑309/+18) | sufA/rydB | Fe‑S cluster assembly protein/novel sRNA, function unknown |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base G (4/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01 | |||||||||||
ATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATC > NC_000913/1764554‑1764842 | aTCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGc < 3:97270/149‑1 (MQ=255) aTTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg < 1:138599/149‑1 (MQ=255) aTGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg > 1:1152812/1‑138 (MQ=255) aTGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg < 2:1152812/138‑1 (MQ=255) tgtgATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGc < 3:461364/105‑1 (MQ=255) tgtgATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGc > 4:461364/1‑105 (MQ=255) gatgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTG‑TCGCACAGACTTaa > 4:1283991/1‑106 (MQ=38) atgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAg > 3:30814/1‑99 (MQ=255) atgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAg < 4:30814/99‑1 (MQ=255) aaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTa > 4:247770/1‑149 (MQ=255) aaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCtt < 3:312261/98‑1 (MQ=255) aaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCtt > 4:312261/1‑98 (MQ=255) ttGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg > 2:1309478/1‑91 (MQ=255) ttGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg < 1:1309478/91‑1 (MQ=255) tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg < 3:247770/149‑1 (MQ=255) tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGgcg < 3:102881/105‑1 (MQ=255) tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGgcg > 4:102881/1‑105 (MQ=255) gagaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTAc > 4:94102/1‑77 (MQ=255) gagaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTAc < 3:94102/77‑1 (MQ=255) aTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGa > 3:151594/1‑128 (MQ=255) aTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGCCGa < 4:151594/128‑1 (MQ=255) aGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGa < 1:234239/118‑1 (MQ=255) aGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGa > 2:234239/1‑118 (MQ=255) ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAc > 3:966345/1‑149 (MQ=255) tAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc > 2:653756/1‑149 (MQ=255) cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa < 3:1337212/49‑1 (MQ=255) cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa > 4:1337212/1‑49 (MQ=255) cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTaa < 3:590049/149‑1 (MQ=255) cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTaa < 1:653756/149‑1 (MQ=255) ttCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtctc > 4:1127983/1‑121 (MQ=255) ttCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtctc < 3:1127983/121‑1 (MQ=255) aaaTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgg > 1:295839/1‑98 (MQ=255) aaaTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgg < 2:295839/98‑1 (MQ=255) aTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg > 1:573097/1‑97 (MQ=255) aTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg < 2:573097/97‑1 (MQ=255) gAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa > 3:198664/1‑102 (MQ=255) gAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa < 4:198664/102‑1 (MQ=255) ccTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtattat > 3:724390/1‑149 (MQ=255) cTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc > 2:941718/1‑88 (MQ=255) cTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc < 1:941718/88‑1 (MQ=255) cTTAAGCCTGTGTCG‑CACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtc < 4:953304/101‑1 (MQ=37) cTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATACGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtc > 3:953304/1‑101 (MQ=37) ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCt > 2:1012377/1‑71 (MQ=255) ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCt < 1:1012377/71‑1 (MQ=255) ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGttt > 3:348133/1‑122 (MQ=255) ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGttt < 4:348133/122‑1 (MQ=255) ttAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctc > 3:1181833/1‑77 (MQ=25) ttAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctc < 4:1181833/77‑1 (MQ=25) ttAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg > 3:606378/1‑83 (MQ=25) ttAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg < 4:606378/83‑1 (MQ=37) aaGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACTCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttttt < 4:966345/149‑1 (MQ=255) aaGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTg > 1:772552/1‑66 (MQ=255) aaGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTg < 2:772552/66‑1 (MQ=255) gTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt > 3:598450/1‑45 (MQ=255) gTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt < 4:598450/45‑1 (MQ=255) tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt > 4:896625/1‑44 (MQ=255) tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt < 3:896625/44‑1 (MQ=255) gACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc < 4:724390/149‑1 (MQ=255) acacAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTa > 4:930677/1‑127 (MQ=255) acacAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTa < 3:930677/127‑1 (MQ=255) | ATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATC > NC_000913/1764554‑1764842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |