Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,797,388 G→T 14.4% P186Q (CCG→CAG)  pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,797,3880GT14.4% 402.3 / 49.8 125P186Q (CCG→CAG) pheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (54/53);  new base T (8/10);  total (62/63)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-02

Only 100 of 128 total aligned reads displayed.

TTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGC  >  NC_000913/1797240‑1797534
                                                                                                                                                    |                                                                                                                                                  
tttcATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                    >  1:505830/1‑149 (MQ=255)
     tCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGc                                                                                                                                               >  4:703433/1‑149 (MQ=255)
      ccACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCt                                                                                                                                              >  4:1183307/1‑149 (MQ=255)
       cacaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTg                                                                                                                                             >  4:1464194/1‑149 (MQ=255)
        acaGCGGCGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTgg                                                                                                                                            <  1:342189/149‑1 (MQ=255)
        acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTgg                                                                                                                                            <  3:767313/149‑1 (MQ=255)
        acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCa                                                                                                                                                 >  4:297109/1‑144 (MQ=255)
        acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCa                                                                                                                                                 <  3:297109/144‑1 (MQ=255)
        acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                            <  3:1464194/149‑1 (MQ=255)
          aGCGGAGTTGGCGCTTTAGCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGtt                                                                                                                                          >  4:551274/1‑149 (MQ=255)
          aGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                          <  3:1183307/149‑1 (MQ=255)
           gCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTc                                                                                                                                         >  2:857993/1‑149 (MQ=255)
              gAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaac                                                                                                                                      <  1:857993/149‑3 (MQ=255)
                         ttAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGt                                                                                                                                           <  1:1085847/133‑1 (MQ=255)
                         ttAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGt                                                                                                                                           >  2:1085847/1‑133 (MQ=255)
                           aaCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGtt                                                                                                                                          >  3:1265856/1‑132 (MQ=255)
                            aCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcg                                                                                                                        >  2:1342254/1‑149 (MQ=255)
                             cGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcgc                                                                                                                       <  3:703433/149‑1 (MQ=255)
                              gTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcgcg                                                                                                                      >  4:450047/1‑149 (MQ=255)
                              gTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGCCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGTACGGCAACGTCAcgcg                                                                                                                      >  4:877614/1‑149 (MQ=255)
                                   tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                          >  3:878313/1‑124 (MQ=255)
                                   tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                          <  4:878313/124‑1 (MQ=255)
                                   tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTc                                                                                                                                         <  1:626390/125‑1 (MQ=255)
                                   tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTc                                                                                                                                         >  2:626390/1‑125 (MQ=255)
                                   tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAaca                                                                                                                 >  2:1260907/1‑149 (MQ=255)
                                    gCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                                                                <  3:450047/149‑1 (MQ=255)
                                       ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                             >  3:123407/1‑149 (MQ=255)
                                               cacGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                                                                >  1:379075/1‑138 (MQ=255)
                                                   gCCAAGATAACGCGGGCAGGCTTCCGGCGCATCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGc                                                                                                 <  3:877614/149‑1 (MQ=255)
                                                    ccAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGc                                                                                                                                               <  3:325305/102‑1 (MQ=255)
                                                    ccAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                            <  4:668518/105‑1 (MQ=255)
                                                    ccAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCa                                                                                                >  3:1433536/1‑149 (MQ=255)
                                                     cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                          >  2:278482/1‑106 (MQ=255)
                                                     cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                          <  1:278482/106‑1 (MQ=255)
                                                      aaGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGt                                                                                              >  4:599922/1‑149 (MQ=255)
                                                         aTAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCgg                                                                                           <  2:505830/149‑1 (MQ=255)
                                                           aaCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCa                                                                                         >  2:1152811/1‑149 (MQ=255)
                                                            aCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCgg                                                                                                                                                   <  3:176339/90‑1 (MQ=255)
                                                                 ggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGttt                                                                                   >  3:660372/1‑149 (MQ=255)
                                                                  gCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTg                                                                                  <  4:123407/149‑1 (MQ=255)
                                                                    aGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGc                                                                                >  3:625090/1‑149 (MQ=255)
                                                                      gCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGt                                                                                     <  3:669964/142‑1 (MQ=255)
                                                                      gCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGt                                                                                     >  4:669964/1‑142 (MQ=255)
                                                                      gCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaacc                                                                                                                                     <  4:453612/94‑4 (MQ=38)
                                                                      gCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaacc                                                                                                                                     >  3:453612/1‑91 (MQ=38)
                                                                             gcgcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                         <  1:453047/115‑1 (MQ=255)
                                                                             gcgcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                         >  2:453047/1‑115 (MQ=255)
                                                                             gcgcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTg                                                                       <  4:1433536/149‑1 (MQ=255)
                                                                               gcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                         >  3:1303527/1‑113 (MQ=255)
                                                                               gcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                         <  4:1303527/113‑1 (MQ=255)
                                                                                 ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAttt                                                                   >  2:256926/1‑149 (MQ=255)
                                                                                 ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAttt                                                                   <  1:1342254/149‑1 (MQ=255)
                                                                                       tGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                                                             >  3:609670/1‑149 (MQ=255)
                                                                                          aaTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTg                                                                                  <  4:532999/125‑1 (MQ=255)
                                                                                                       tcgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGtt                                                                                                                                          >  3:461019/1‑56 (MQ=255)
                                                                                                       tcgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGttt                                             >  4:267069/1‑149 (MQ=255)
                                                                                                         gtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                           >  3:22375/1‑149 (MQ=255)
                                                                                                         gtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                           >  1:174155/1‑149 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGAt                                                               <  1:313469/128‑1 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGAt                                                               >  2:313469/1‑128 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                           <  1:625580/148‑1 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                           >  2:627777/1‑148 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                           >  2:625580/1‑148 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                           <  1:627777/148‑1 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          <  3:267069/149‑1 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          <  1:1152811/149‑1 (MQ=255)
                                                                                                          tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          <  3:551274/149‑1 (MQ=255)
                                                                                                                  cGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                         >  3:46270/1‑78 (MQ=255)
                                                                                                                  cGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                         <  4:46270/78‑1 (MQ=255)
                                                                                                                  cGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCAc                                  <  4:625090/149‑1 (MQ=255)
                                                                                                                  cGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCAc                                  <  4:433378/149‑1 (MQ=255)
                                                                                                                     aCCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          >  2:666639/1‑138 (MQ=255)
                                                                                                                      ccAACCGGATCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGat                              >  1:401992/1‑149 (MQ=255)
                                                                                                                        aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttgt                                                       <  4:889406/122‑1 (MQ=255)
                                                                                                                        aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttgt                                                       >  3:889406/1‑122 (MQ=255)
                                                                                                                        aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                            >  1:85586/1‑133 (MQ=255)
                                                                                                                            ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                        <  4:22375/149‑1 (MQ=255)
                                                                                                                            ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                        >  1:235071/1‑149 (MQ=255)
                                                                                                                            ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                        <  2:346964/149‑1 (MQ=255)
                                                                                                                             gAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGc                                                                                >  4:1895/1‑92 (MQ=255)
                                                                                                                             gAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTg                       >  4:106858/1‑149 (MQ=255)
                                                                                                                              atcGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                      <  2:401992/147‑1 (MQ=255)
                                                                                                                                       cGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                            >  2:899328/1‑118 (MQ=255)
                                                                                                                                       cGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                            <  1:899328/118‑1 (MQ=255)
                                                                                                                                        tgctGAACCAGCTGCCGCTGGTTCAACACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          >  2:955265/4‑119 (MQ=255)
                                                                                                                                        tgctGAACCAGCTGCCGCTGGTTCAACACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          <  1:955265/116‑1 (MQ=255)
                                                                                                                                         gTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                          <  1:2547/118‑1 (MQ=255)
                                                                                                                                         gTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCa           >  3:663690/1‑149 (MQ=255)
                                                                                                                                             aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc                                                                           <  1:1176556/81‑1 (MQ=255)
                                                                                                                                             aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc                                                                           >  2:1176556/1‑81 (MQ=255)
                                                                                                                                             aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                            <  3:549507/112‑1 (MQ=255)
                                                                                                                                             aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                            >  4:549507/1‑112 (MQ=255)
                                                                                                                                               ccAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                      >  3:723170/1‑132 (MQ=255)
                                                                                                                                               ccAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                      <  4:723170/132‑1 (MQ=255)
                                                                                                                                               ccAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgc      <  1:111862/148‑1 (MQ=255)
                                                                                                                                               ccAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgc      >  2:111862/1‑148 (MQ=255)
                                                                                                                                                cAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcag    <  4:609670/149‑1 (MQ=255)
                                                                                                                                                  gCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgtt                                                         <  4:1423119/94‑1 (MQ=255)
                                                                                                                                                  gCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcaggc  <  2:235071/149‑1 (MQ=255)
                                                                                                                                                   cGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCaa                                                 >  3:59759/1‑101 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGC  >  NC_000913/1797240‑1797534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: