Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,425 A→C 5.2% intergenic (‑93/+277) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4250AC 5.2% 712.2 / 19.7 190intergenic (‑93/+277)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base A (93/87);  new base C (5/5);  total (98/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATT  >  NC_000913/1861278‑1861573
                                                                                                                                                   |                                                                                                                                                    
     atcatcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                <  3:1181558/149‑1 (MQ=255)
     atcatcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                >  1:735931/1‑149 (MQ=255)
     atcatcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                <  2:735931/149‑1 (MQ=255)
      tcatcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCt                                                                                                                                               <  2:998423/149‑1 (MQ=255)
            tGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTTCCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGttt                                                                                                                                         <  1:111107/149‑1 (MQ=255)
                    ccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                                                                                 >  2:296966/1‑149 (MQ=255)
                     cAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                                <  1:989414/149‑1 (MQ=255)
                     cAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                                <  1:26685/149‑1 (MQ=255)
                       aaGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGa                                                                                                                              >  2:403152/1‑149 (MQ=255)
                            cTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                <  2:1456137/126‑1 (MQ=255)
                                ccAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                                                                     >  1:787151/1‑149 (MQ=255)
                                 cAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                                                                           >  4:1224024/1‑126 (MQ=255)
                                 cAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                                                                           <  3:1224024/126‑1 (MQ=255)
                                    aCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCa                                                                                                                 >  2:1002305/1‑149 (MQ=255)
                                          ccAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                           >  4:990788/1‑149 (MQ=255)
                                             gTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                                                                            <  1:1331657/129‑1 (MQ=255)
                                             gTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                                                                            >  2:1331657/1‑129 (MQ=255)
                                              tGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                           >  2:310092/1‑145 (MQ=255)
                                                cTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTgg                                                                                                     <  2:787151/149‑1 (MQ=255)
                                                cTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTgg                                                                                                     <  1:982963/149‑1 (MQ=255)
                                                    cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATTGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATaa                                                                                                                                                     <  1:130931/97‑1 (MQ=255)
                                                    cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAAAATTAAATCTGTAAAACACAGTTaa                                                                                                                                                     >  2:130931/1‑97 (MQ=255)
                                                     aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTAc                                                                                                                    <  2:1243945/129‑1 (MQ=255)
                                                     aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                                                                                <  1:156188/149‑1 (MQ=255)
                                                      ttGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                                       >  3:997317/1‑141 (MQ=255)
                                                           tCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAg                                                                                          >  3:1019033/1‑149 (MQ=255)
                                                             aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                                        >  4:743619/1‑149 (MQ=255)
                                                             aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                                        >  1:451028/1‑149 (MQ=255)
                                                              tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGATGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTATGCAGAAAATGGca                                                                                                       <  4:946810/133‑2 (MQ=255)
                                                              tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                                                                       >  1:1238097/1‑149 (MQ=255)
                                                              tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                                                                       >  1:317473/1‑149 (MQ=255)
                                                                 tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCATCATTAAATCTGTAAAACGCAGATCATTATCTGTGtt                                                                                                                                          >  4:794586/1‑95 (MQ=38)
                                                                 tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGtt                                                                                                                                          <  3:794586/95‑1 (MQ=38)
                                                                  ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   <  3:956839/149‑1 (MQ=255)
                                                                  ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   <  3:743619/149‑1 (MQ=255)
                                                                      gtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                                                                               <  4:1019033/149‑1 (MQ=255)
                                                                       tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGttt                                                                                                                                         >  3:507107/1‑90 (MQ=255)
                                                                       tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGttt                                                                                                                                         <  4:507107/90‑1 (MQ=255)
                                                                       tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAgg                                                                              >  2:1193019/1‑149 (MQ=255)
                                                                       tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAgg                                                                              >  4:183076/1‑149 (MQ=255)
                                                                           gTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCggg                                                                          <  1:1002305/149‑1 (MQ=255)
                                                                            ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCt                                                                                                 <  1:508067/125‑1 (MQ=255)
                                                                            ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCt                                                                                                 >  2:508067/1‑125 (MQ=255)
                                                                            ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   >  3:674730/1‑139 (MQ=255)
                                                                            ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   <  4:674730/139‑1 (MQ=255)
                                                                            ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgggg                                                                         >  3:1454860/1‑149 (MQ=255)
                                                                                  ttAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTa                                                                                                                                       <  2:1174725/81‑1 (MQ=255)
                                                                                  ttAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                                                                               >  3:30674/1‑137 (MQ=255)
                                                                                    aGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   <  3:393283/131‑1 (MQ=255)
                                                                                            atcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATg                                                                                                          <  2:1187812/100‑1 (MQ=255)
                                                                                             tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                                       <  1:295906/102‑1 (MQ=255)
                                                                                             tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                                       >  2:295906/1‑102 (MQ=255)
                                                                                              catACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa                                                          >  4:411472/1‑146 (MQ=255)
                                                                                                tACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga                                                     >  2:173519/1‑149 (MQ=255)
                                                                                                  cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   >  4:13952/1‑117 (MQ=255)
                                                                                                  cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   <  3:13952/117‑1 (MQ=255)
                                                                                                  cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa                                                                 >  4:44377/1‑135 (MQ=255)
                                                                                                  cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga                                                     <  1:306990/147‑1 (MQ=255)
                                                                                                   ttACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                    <  4:112579/115‑1 (MQ=255)
                                                                                                   ttACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                    >  3:112579/1‑115 (MQ=255)
                                                                                                       ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGtt                                                                                              >  4:1386939/1‑101 (MQ=255)
                                                                                                       ccACATGATTCTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGtt                                                                                              <  3:1386939/101‑1 (MQ=255)
                                                                                                        cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGAt                                                                                                                             <  3:432069/69‑1 (MQ=255)
                                                                                                            tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAc                                           <  4:339568/147‑1 (MQ=255)
                                                                                                                  ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTATCGGCCaaa                                   <  4:1454860/149‑1 (MQ=255)
                                                                                                                  ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCaaa                                   <  2:451028/149‑1 (MQ=255)
                                                                                                                     aGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAg                                >  1:1250490/1‑149 (MQ=255)
                                                                                                                       gTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                              >  1:106832/1‑149 (MQ=255)
                                                                                                                       gTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                              >  4:1240834/1‑149 (MQ=255)
                                                                                                                        tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                                       <  1:1443437/75‑1 (MQ=255)
                                                                                                                        tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                               >  3:931582/1‑147 (MQ=255)
                                                                                                                         cAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                   >  3:453305/1‑94 (MQ=255)
                                                                                                                          aaCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                               >  4:557787/1‑113 (MQ=255)
                                                                                                                          aaCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                               <  3:557787/113‑1 (MQ=255)
                                                                                                                          aaCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTg                           >  3:508817/1‑149 (MQ=255)
                                                                                                                             aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGGGAGGGATTACCGGCCAAATAGGTCTgaga                        <  1:1190802/149‑1 (MQ=255)
                                                                                                                             aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTgaga                        >  3:1115809/1‑149 (MQ=255)
                                                                                                                              ttAAATCTGTAATATACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                                                              >  3:104671/1‑110 (MQ=255)
                                                                                                                              ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                                                              <  4:104671/110‑1 (MQ=255)
                                                                                                                              ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                       >  2:1088883/1‑149 (MQ=255)
                                                                                                                              ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                       <  2:1439090/149‑1 (MQ=255)
                                                                                                                                aaaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACtt                     <  4:508817/149‑1 (MQ=255)
                                                                                                                                 aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTa                    >  1:88857/1‑149 (MQ=255)
                                                                                                                                  aTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATa                                 <  1:822480/135‑1 (MQ=255)
                                                                                                                                  aTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATa                                 >  2:822480/1‑135 (MQ=255)
                                                                                                                                   tCTGTAAAACACAGATAATGATCTGCGTTTTACAGCTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAg                  >  3:1293796/1‑149 (MQ=255)
                                                                                                                                        aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAg             <  2:88857/149‑1 (MQ=255)
                                                                                                                                          aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                               <  4:494818/97‑1 (MQ=255)
                                                                                                                                          aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa                                                          >  1:1184946/1‑102 (MQ=255)
                                                                                                                                           acgcaGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga                                                   >  3:53734/4‑108 (MQ=255)
                                                                                                                                           acgcaGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga                                                   <  4:53734/105‑1 (MQ=255)
                                                                                                                                           acgcaGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGagag                                                  >  2:589985/4‑109 (MQ=255)
                                                                                                                                           acgcaGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGagag                                                  <  1:589985/106‑1 (MQ=255)
                                                                                                                                             acaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGaa        >  4:256999/1‑149 (MQ=255)
                                                                                                                                                  tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                    >  3:1333918/1‑68 (MQ=255)
                                                                                                                                                  tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                    <  4:1333918/68‑1 (MQ=255)
                                                                                                                                                  tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                               >  1:1273736/1‑89 (MQ=255)
                                                                                                                                                  tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                               <  2:1273736/89‑1 (MQ=255)
                                                                                                                                                   aaTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCGAAAAGAGCAAATTCGAGAGGGATCACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCAtt  >  4:927363/1‑149 (MQ=255)
                                                                                                                                                   aaTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCAtt  <  2:984307/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATT  >  NC_000913/1861278‑1861573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: