Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,428 G→T 5.1% intergenic (‑96/+274) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4280GT 5.1% 709.3 / 16.6 194intergenic (‑96/+274)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base G (95/89);  new base T (5/5);  total (100/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 199 total aligned reads displayed.

CAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAG  >  NC_000913/1861281‑1861576
                                                                                                                                                   |                                                                                                                                                    
caatcaTCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGa                                                                                                                                                     >  1:998423/1‑149 (MQ=255)
 aatcaTCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAt                                                                                                                                                    >  2:156188/1‑149 (MQ=255)
  atcatcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                   <  3:1181558/149‑1 (MQ=255)
  atcatcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                   <  2:735931/149‑1 (MQ=255)
     atcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGc                                                                                                                                                >  2:1330651/1‑149 (MQ=255)
      tcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                                                                               >  4:1231488/1‑149 (MQ=255)
       cTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTATATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                                                                              >  4:956839/1‑149 (MQ=255)
         tGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTTCCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGttt                                                                                                                                            <  1:111107/149‑1 (MQ=255)
                gccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGtt                                                                                                                                             >  3:524856/1‑141 (MQ=255)
                 ccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                                                                                    >  2:296966/1‑149 (MQ=255)
                  cAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                                   <  1:296966/149‑1 (MQ=255)
                    aaGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGa                                                                                                                                 >  2:403152/1‑149 (MQ=255)
                         cTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACACTAAATCTGTAAAACACAGATAATGATc                                                                                                                                                   >  1:1456137/1‑126 (MQ=255)
                          ttGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACaa                                                                                                                           >  3:507323/1‑149 (MQ=255)
                              cAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                                                                              <  3:1224024/126‑1 (MQ=255)
                               aaaCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                                                                                      >  3:1377738/1‑149 (MQ=255)
                                 aCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCa                                                                                                                    <  1:1330651/149‑1 (MQ=255)
                                       ccAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                              <  1:527919/149‑1 (MQ=255)
                                       ccAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                              >  4:990788/1‑149 (MQ=255)
                                        cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTAc                                                                                                                       >  1:1354469/1‑139 (MQ=255)
                                        cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTAc                                                                                                                       <  2:1354469/139‑1 (MQ=255)
                                          gTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGc                                                                                                           <  1:403152/149‑1 (MQ=255)
                                           tGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                              <  1:310092/145‑1 (MQ=255)
                                           tGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                              >  2:310092/1‑145 (MQ=255)
                                             cTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTgg                                                                                                        <  2:787151/149‑1 (MQ=255)
                                                  aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTAc                                                                                                                       <  2:1243945/129‑1 (MQ=255)
                                                  aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                                                                                   >  3:760651/1‑149 (MQ=255)
                                                  aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                                                                                   >  2:276200/1‑149 (MQ=255)
                                                       gTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGc                                                                                                           <  1:889720/136‑1 (MQ=255)
                                                          aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                                           >  4:743619/1‑149 (MQ=255)
                                                           tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                                                                          >  1:1238097/1‑149 (MQ=255)
                                                              tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCATCATTAAATCTGTAAAACGCAGATCATTATCTGTGtt                                                                                                                                             >  4:794586/1‑95 (MQ=38)
                                                              tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACaa                                                                                                                                       <  4:880239/101‑1 (MQ=255)
                                                              tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       <  3:990788/149‑1 (MQ=255)
                                                              tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       >  1:625022/1‑149 (MQ=255)
                                                               ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                      <  3:956839/149‑1 (MQ=255)
                                                                    tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGttt                                                                                                                                            <  4:507107/90‑1 (MQ=255)
                                                                    tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAgg                                                                                 >  2:1193019/1‑149 (MQ=255)
                                                                    tttAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAgg                                                                                 >  4:183076/1‑149 (MQ=255)
                                                                      tAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCg                                                                               <  3:183076/149‑1 (MQ=255)
                                                                        gTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCggg                                                                             <  1:1002305/149‑1 (MQ=255)
                                                                         ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCt                                                                                                    >  2:508067/1‑125 (MQ=255)
                                                                         ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       >  3:1394533/1‑138 (MQ=255)
                                                                         ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                      <  4:674730/139‑1 (MQ=255)
                                                                               ttAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTa                                                                                                                                          <  2:1174725/81‑1 (MQ=255)
                                                                                 aGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcgc                                                                         >  4:1013356/1‑144 (MQ=255)
                                                                                 aGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcgc                                                                         <  3:1013356/144‑1 (MQ=255)
                                                                                         atcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATg                                                                                                             <  2:1187812/100‑1 (MQ=255)
                                                                                          tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGAt                                                                                                                                <  1:778238/80‑1 (MQ=255)
                                                                                           catACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa                                                             <  3:411472/146‑1 (MQ=255)
                                                                                           catACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa                                                             >  4:411472/1‑146 (MQ=255)
                                                                                             tACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga                                                        <  1:173519/149‑1 (MQ=255)
                                                                                             tACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga                                                        <  1:488593/149‑1 (MQ=255)
                                                                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                      >  4:13952/1‑117 (MQ=255)
                                                                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa                                                                    >  4:44377/1‑135 (MQ=255)
                                                                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa                                                                    <  3:44377/135‑1 (MQ=255)
                                                                                                ttACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       <  4:112579/115‑1 (MQ=255)
                                                                                                ttACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       >  3:112579/1‑115 (MQ=255)
                                                                                                    ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGtt                                                                                                 >  4:1386939/1‑101 (MQ=255)
                                                                                                    ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGAt                                                 >  1:984307/1‑149 (MQ=255)
                                                                                                       caTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAc                                              <  1:276200/149‑1 (MQ=255)
                                                                                                        aTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga                                                        <  3:590377/138‑1 (MQ=255)
                                                                                                         tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAc                                              >  3:339568/1‑147 (MQ=255)
                                                                                                         tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAc                                              <  4:339568/147‑1 (MQ=255)
                                                                                                              tttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgggg                                                                            >  1:790469/1‑112 (MQ=255)
                                                                                                               ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTATCGGCCaaa                                      <  4:1454860/149‑1 (MQ=255)
                                                                                                               ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCaaa                                      <  2:451028/149‑1 (MQ=255)
                                                                                                                  aGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAg                                   >  1:1250490/1‑149 (MQ=255)
                                                                                                                    gTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                                 >  1:106832/1‑149 (MQ=255)
                                                                                                                     tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                                          >  2:1443437/1‑75 (MQ=255)
                                                                                                                     tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       >  1:831804/1‑94 (MQ=255)
                                                                                                                     tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                                  >  3:931582/1‑147 (MQ=255)
                                                                                                                     tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                                  <  4:931582/147‑1 (MQ=255)
                                                                                                                       aaCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                                  >  4:557787/1‑113 (MQ=255)
                                                                                                                          aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGGGAGGGATTACCGGCCAAATAGGTCTgaga                           <  1:1190802/149‑1 (MQ=255)
                                                                                                                          aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTgaga                           >  3:1443005/1‑149 (MQ=255)
                                                                                                                          aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTgaga                           >  3:1115809/1‑149 (MQ=255)
                                                                                                                           ttAAATCTGTAATATACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                                                                 >  3:104671/1‑110 (MQ=255)
                                                                                                                           ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                                                                 <  4:104671/110‑1 (MQ=255)
                                                                                                                           ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                          >  4:1311262/1‑149 (MQ=255)
                                                                                                                           ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                          <  3:686058/149‑1 (MQ=255)
                                                                                                                           ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                          >  2:1088883/1‑149 (MQ=255)
                                                                                                                             aaaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACtt                        <  4:508817/149‑1 (MQ=255)
                                                                                                                               aTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATa                                    >  2:822480/1‑135 (MQ=255)
                                                                                                                                tCTGTAAAACACAGATAATGATCTGCGTTTTACAGCTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAg                     >  3:1293796/1‑149 (MQ=255)
                                                                                                                                     aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAg                <  2:88857/149‑1 (MQ=255)
                                                                                                                                       aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                                  <  4:494818/97‑1 (MQ=255)
                                                                                                                                       aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                                  >  3:494818/1‑97 (MQ=255)
                                                                                                                                       aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa                                                             >  1:1184946/1‑102 (MQ=255)
                                                                                                                                       aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGga              <  3:555218/149‑1 (MQ=255)
                                                                                                                                        acgcaGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga                                                      <  4:53734/105‑1 (MQ=255)
                                                                                                                                        acgcaGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGagag                                                     >  2:589985/4‑109 (MQ=255)
                                                                                                                                          acaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGaa           >  4:256999/1‑149 (MQ=255)
                                                                                                                                               tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                       <  4:1333918/68‑1 (MQ=255)
                                                                                                                                               tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                                  >  1:1273736/1‑89 (MQ=255)
                                                                                                                                               tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                                  <  2:1273736/89‑1 (MQ=255)
                                                                                                                                                aaTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCAtt     <  2:984307/149‑1 (MQ=255)
                                                                                                                                                  tGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTaa                      >  3:822328/1‑130 (MQ=255)
                                                                                                                                                   gATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAg  >  4:1217415/1‑149 (MQ=255)
                                                                                                                                                   gATCTGAGTTGTAAAACGCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                                                                      <  3:1314821/65‑1 (MQ=25)
                                                                                                                                                   |                                                                                                                                                    
CAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAG  >  NC_000913/1861281‑1861576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: