Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,872,131 A→G 7.8% S31G (AGC→GGC)  yeaJ → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,872,1310AG 7.8% 371.7 / 16.0 115S31G (AGC→GGC) yeaJputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (51/55);  new base G (5/4);  total (56/59)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

Only 100 of 118 total aligned reads displayed.

CGAACTTTATCTGGTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCG  >  NC_000913/1871990‑1872279
                                                                                                                                             |                                                                                                                                                    
cGAACTTTATCTGGTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACag                                                                                                                                               <  4:192648/149‑1 (MQ=255)
     tttATCTGGTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCaa                                                                                                                                          <  4:428576/149‑1 (MQ=255)
     tttATCTGGTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCaa                                                                                                                                          >  1:538079/1‑149 (MQ=255)
         tCTGGTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAg                                                                                                                                      <  1:149353/149‑1 (MQ=255)
             gTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGa                                                                                                                                       >  3:1056428/1‑144 (MQ=255)
             gTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGa                                                                                                                                       <  4:1056428/144‑1 (MQ=255)
                  ctcGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCTGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCt                                                                                                                             <  2:367494/149‑1 (MQ=255)
                      tttCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTgcgc                                                                                                                         >  2:1015162/1‑149 (MQ=255)
                          aCTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCtata                                                                                                                     >  2:488218/1‑149 (MQ=255)
                             aaCCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCg                                                                                                                  <  1:599370/149‑1 (MQ=255)
                                cGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGc                                                                                                               >  3:986653/1‑149 (MQ=255)
                                   aGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGa                                                                                                            >  3:1208180/1‑149 (MQ=255)
                                    ggAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGaa                                                                                                           >  3:814748/1‑149 (MQ=255)
                                        tGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGa                                                                                                                                                  >  3:580513/1‑106 (MQ=255)
                                          ccATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACag                                                                                                                                               >  2:121050/1‑107 (MQ=255)
                                          ccATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACag                                                                                                                                               <  1:121050/107‑1 (MQ=255)
                                           cATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGt                                                                                                                                     <  1:214626/116‑1 (MQ=255)
                                            aTTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGAct                                                                                                   >  2:377456/1‑149 (MQ=255)
                                             tttATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGActc                                                                                                  >  2:109313/1‑149 (MQ=255)
                                                aTCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGActcctc                                                                                               <  1:109313/149‑1 (MQ=255)
                                                   aTGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAg                                                                                                                                      <  1:814809/107‑1 (MQ=255)
                                                   aTGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGt                                                                                                                                     >  4:601454/1‑108 (MQ=255)
                                                   aTGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGt                                                                                                                                     <  3:601454/108‑1 (MQ=255)
                                                     gAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCctttc                                                                                          <  4:616847/149‑1 (MQ=255)
                                                          tGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTAt                                                                                     <  4:1208180/149‑1 (MQ=255)
                                                             aCCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTgcgc                                                                                                                         >  1:594215/1‑110 (MQ=255)
                                                             aCCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTgcgc                                                                                                                         <  2:594215/110‑1 (MQ=255)
                                                               cATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGc                                                                                                                                            <  3:200435/89‑1 (MQ=255)
                                                               cATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGa                                                                                                     <  4:746423/128‑1 (MQ=255)
                                                               cATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGa                                                                                                     >  3:746423/1‑128 (MQ=255)
                                                               cATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGAt                                                                                  >  1:350979/1‑147 (MQ=255)
                                                               cATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGAt                                                                                  <  2:350979/147‑1 (MQ=255)
                                                                 tAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAAt                                                                                                                                         <  3:212187/90‑1 (MQ=255)
                                                                 tAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAAt                                                                                                                                         >  4:212187/1‑90 (MQ=255)
                                                                    aaTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAAt                                                                                                                                         <  3:1080834/87‑1 (MQ=255)
                                                                    aaTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAAt                                                                                                                                         >  4:1080834/1‑87 (MQ=255)
                                                                    aaTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGa                                                                                   >  2:1402162/1‑141 (MQ=255)
                                                                    aaTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGa                                                                                   <  1:1402162/141‑1 (MQ=255)
                                                                    aaTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatc                                                                           <  1:488218/149‑1 (MQ=255)
                                                                     aTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATaa                                                                                <  2:988081/143‑1 (MQ=255)
                                                                     aTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATaa                                                                                >  1:988081/1‑143 (MQ=255)
                                                                     aTGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatca                                                                          <  4:814748/149‑1 (MQ=255)
                                                                         tCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGc                                                                                                                                   >  2:741286/1‑88 (MQ=255)
                                                                         tCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaat                                                                      >  1:1260502/1‑149 (MQ=255)
                                                                           cGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCTGGCGACaa                                                                                                                                               >  1:1403060/1‑73 (MQ=255)
                                                                           cGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCTGGCGACaa                                                                                                                                               <  2:1403060/74‑2 (MQ=255)
                                                                             gCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  <  4:968424/149‑1 (MQ=255)
                                                                                ttttATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAAt                                                                                                                                         <  3:1383526/75‑1 (MQ=255)
                                                                                ttttATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAAt                                                                                                                                         >  4:1383526/1‑75 (MQ=255)
                                                                                    aTCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGc                                                                                                                                   >  2:192219/1‑77 (MQ=255)
                                                                                    aTCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGc                                                           <  2:1260502/149‑1 (MQ=255)
                                                                                    aTCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGc                                                           <  1:1015162/149‑1 (MQ=255)
                                                                                     tCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGcc                                                          >  2:1080609/1‑149 (MQ=255)
                                                                                        ccgcAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCa                                                                                                              <  1:766239/94‑1 (MQ=255)
                                                                                        ccgcAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCa                                                                                                              >  2:766239/1‑94 (MQ=255)
                                                                                        ccgcAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCcgcg                                                       >  1:154985/1‑149 (MQ=255)
                                                                                               tgtCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAtgt                                                  <  3:983105/147‑1 (MQ=255)
                                                                                               tgtCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAtgt                                                  >  4:983105/1‑147 (MQ=255)
                                                                                               tgtCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGa                                                >  1:363279/1‑149 (MQ=255)
                                                                                                   aTTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATgc                                             >  3:711355/1‑148 (MQ=255)
                                                                                                   aTTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATgc                                             <  4:711355/148‑1 (MQ=255)
                                                                                                    ttGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCa                                                                                                              >  2:853844/1‑82 (MQ=255)
                                                                                                    ttGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCa                                                                                                              <  1:853844/82‑1 (MQ=255)
                                                                                                       tgCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGct                                        >  1:395286/1‑149 (MQ=255)
                                                                                                          tGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCctttc                                                                                          <  2:1206132/96‑1 (MQ=255)
                                                                                                          tGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCctttc                                                                                          >  1:1206132/1‑96 (MQ=255)
                                                                                                          tGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCtctc                                     <  1:1080609/149‑1 (MQ=255)
                                                                                                            aCATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgc                                   <  2:395286/149‑1 (MQ=255)
                                                                                                             cATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgct                                  <  2:154985/149‑1 (MQ=255)
                                                                                                               tCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCgctgc                                <  2:363279/149‑1 (MQ=255)
                                                                                                                 ttccttccTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGc                                                                                                                                   <  4:1360443/48‑1 (MQ=255)
                                                                                                                 ttccttccTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCcttt                                                                                           <  3:601429/88‑1 (MQ=255)
                                                                                                                 ttccttccTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCcttt                                                                                           >  4:601429/1‑88 (MQ=255)
                                                                                                                     ttccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGt                                                                                                                 >  3:1425190/1‑62 (MQ=255)
                                                                                                                     ttccttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGt                                                                                                                 <  4:1425190/62‑1 (MQ=255)
                                                                                                                        cttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGaaa                                                                                                          >  3:771460/1‑66 (MQ=255)
                                                                                                                        cttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGaaa                                                                                                          <  4:771460/66‑1 (MQ=255)
                                                                                                                        cttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTa                                                                                      >  4:9095/1‑86 (MQ=255)
                                                                                                                        cttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTa                                                                                      <  3:9095/86‑1 (MQ=255)
                                                                                                                         ttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAg                                                                                                         <  2:1224207/66‑1 (MQ=255)
                                                                                                                         ttATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGActcc                                                                                                 <  3:1341056/74‑1 (MQ=255)
                                                                                                                          tATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGc                                                                                                                                   <  3:199588/39‑1 (MQ=255)
                                                                                                                          tATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGc                                                                                                                                   >  4:199588/1‑39 (MQ=255)
                                                                                                                             ttttGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCtctc                                     <  4:647770/130‑1 (MQ=255)
                                                                                                                             ttttGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCtctc                                     >  3:647770/1‑130 (MQ=255)
                                                                                                                                  gctctGTCGCTGGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  >  2:703862/5‑96 (MQ=255)
                                                                                                                                  gctctGTCGCTGGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  <  1:703862/92‑1 (MQ=255)
                                                                                                                                    tctGTCGCTGGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  <  2:210097/92‑1 (MQ=255)
                                                                                                                                    tctGTCGCTGGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  >  1:210097/3‑94 (MQ=255)
                                                                                                                                    tctGTCGCTGGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  >  1:1055095/3‑94 (MQ=255)
                                                                                                                                     ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCctttcttt                                                                                       <  1:1374719/72‑1 (MQ=255)
                                                                                                                                     ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATaa                                                                                >  4:1462201/1‑79 (MQ=255)
                                                                                                                                     ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATaa                                                                                <  3:1462201/79‑1 (MQ=255)
                                                                                                                                     ttGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCa          >  1:23563/1‑149 (MQ=255)
                                                                                                                                           ccAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatcagaatcaga                                                                  <  4:339539/87‑1 (MQ=255)
                                                                                                                                           ccAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatca                                                                          <  4:55733/79‑1 (MQ=255)
                                                                                                                                           ccAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTatca                                                                          >  3:55733/1‑79 (MQ=255)
                                                                                                                                             aGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCg                                    <  4:391011/115‑1 (MQ=255)
                                                                                                                                             aGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCg                                    >  3:391011/1‑115 (MQ=255)
                                                                                                                                             aGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAgcg  >  2:62888/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CGAACTTTATCTGGTTTTCTCGTTTCACTAACCGAAGGAGTGCCATTTATCATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGAACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCG  >  NC_000913/1871990‑1872279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: