Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,880,851 T→A 5.7% intergenic (‑92/+35) leuE ← / ← dmlR leucine efflux protein/transcriptional activator of dmlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,880,8510TA 5.7% 316.5 / 7.6 89intergenic (‑92/+35)leuE/dmlRleucine efflux protein/transcriptional activator of dmlA
Reads supporting (aligned to +/- strand):  ref base T (41/42);  new base A (2/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

AATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAAC  >  NC_000913/1880706‑1880993
                                                                                                                                                 |                                                                                                                                              
aaTGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTaa                                                                                                                                             <  3:788056/149‑1 (MQ=255)
      cccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                       <  3:1359658/149‑1 (MQ=255)
      cccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                       >  4:1359658/1‑149 (MQ=255)
       ccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAAGCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                       >  4:921583/1‑148 (MQ=255)
       ccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGat                                                                                                                                       <  3:921583/148‑1 (MQ=255)
       ccAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                                                                      <  1:24881/149‑2 (MQ=255)
              gATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATGTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTCTAACCTGAGATcggaag                                                                                                                               >  4:1236521/1‑144 (MQ=255)
                  ggTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATa                                                                                                                           <  1:935928/149‑1 (MQ=255)
                  ggTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATa                                                                                                                           >  4:472093/1‑149 (MQ=255)
                      cAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTg                                                                                                                       >  1:116110/1‑149 (MQ=255)
                      cAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTg                                                                                                                       >  2:962703/1‑149 (MQ=255)
                        gTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGaa                                                                                                                     <  1:419818/149‑1 (MQ=255)
                            ttCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCAt                                                                                                                 >  4:806622/1‑149 (MQ=255)
                               aGAACCCCGTATTCAGCGAACACATTTACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGACAATCAGGTTTTAACCTGATATCAACCCGATAAtt                                                                                                                        <  3:973679/139‑1 (MQ=255)
                               aGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAtt                                                                                                                        >  4:973679/1‑139 (MQ=255)
                               aGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTaa                                                                                                              <  3:102869/149‑1 (MQ=255)
                               aGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTaa                                                                                                              <  2:116110/149‑1 (MQ=255)
                                   ccccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTg                                                                                                                                         >  4:684159/1‑118 (MQ=255)
                                   ccccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTg                                                                                                                                         <  3:684159/118‑1 (MQ=255)
                                    cccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAtt                                                                                                                        <  4:157709/134‑1 (MQ=255)
                                    cccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAtt                                                                                                                        >  3:157709/1‑134 (MQ=255)
                                    cccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAgg                                                                                                         <  3:806622/149‑1 (MQ=255)
                                     ccGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGc                                                                                                        >  3:1248684/1‑149 (MQ=255)
                                      cGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAcc                                                                                                                                >  3:437882/1‑124 (MQ=255)
                                      cGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAcc                                                                                                                                <  4:437882/124‑1 (MQ=255)
                                           tCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTc                                                                                                  <  4:1248684/149‑1 (MQ=255)
                                                 aaCACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGc                                                                                                    <  2:1434046/141‑1 (MQ=255)
                                                 aaCACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGc                                                                                                    >  1:1434046/1‑141 (MQ=255)
                                                   cacaTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATc                                                                                                            >  3:77558/1‑131 (MQ=255)
                                                   cacaTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATc                                                                                                            <  4:77558/131‑1 (MQ=255)
                                                      aTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGtttt                                                                                                                                               >  4:664244/1‑93 (MQ=255)
                                                      aTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGtttt                                                                                                                                               <  3:664244/93‑1 (MQ=255)
                                                        tAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTc                                                                                     <  3:472093/149‑1 (MQ=255)
                                                           ccTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg                                                                                  <  1:962703/149‑1 (MQ=255)
                                                            ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGaaa                                                                                          >  4:1263404/1‑140 (MQ=255)
                                                            ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGaaa                                                                                          <  3:1263404/140‑1 (MQ=255)
                                                             tctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTa                                                                                >  4:999334/1‑149 (MQ=255)
                                                             tctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                                                                      <  3:1229210/95‑2 (MQ=255)
                                                             tctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                                                                      >  4:1229210/1‑94 (MQ=255)
                                                                   aTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg                                                                                   >  3:919317/1‑140 (MQ=255)
                                                                   aTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg                                                                                   <  4:919317/140‑1 (MQ=255)
                                                                    ttATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCg                                                                         >  1:577478/1‑149 (MQ=255)
                                                                      aTCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCa                                                                                                           <  1:1385664/113‑1 (MQ=255)
                                                                      aTCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCa                                                                                                           >  2:1385664/1‑113 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGa                                                                                                                                        >  1:1337084/1‑81 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGa                                                                                                                                        <  2:1337084/81‑1 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAc                                                                                                                                 <  4:73739/88‑1 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAc                                                                                                                                 >  3:73739/1‑88 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATACTTGAATCAGTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                    >  3:1261677/1‑149 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt                                                                                                                    <  4:69985/101‑1 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt                                                                                                                    >  3:69985/1‑101 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                    >  3:1306621/1‑149 (MQ=255)
                                                                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                                                                    >  1:645963/1‑149 (MQ=255)
                                                                          ttCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaaa                                                                   <  2:645963/149‑1 (MQ=255)
                                                                             gATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATAc                                                                >  2:692961/1‑149 (MQ=255)
                                                                                   gcgcGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCa                                                                                                           <  3:317479/100‑1 (MQ=255)
                                                                                   gcgcGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCa                                                                                                           >  4:317479/1‑100 (MQ=255)
                                                                                    cgcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTa                                                         <  1:692961/149‑1 (MQ=255)
                                                                                       gATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaa                                                      >  3:1066121/1‑149 (MQ=255)
                                                                                             aGGTGAATATGCTAACCAATCTGTAGCGGCTTAGACAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCACTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTa                                                <  3:999334/149‑1 (MQ=255)
                                                                                              ggTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTAc                                               <  4:1306621/149‑1 (MQ=255)
                                                                                               gTGAATTTGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                                                                      <  4:621243/61‑2 (MQ=38)
                                                                                               gTGAATTTGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                                                                      >  3:621243/1‑60 (MQ=38)
                                                                                               gTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGt                                                                                 <  2:284188/114‑1 (MQ=255)
                                                                                               gTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGt                                                                                 >  1:284188/1‑114 (MQ=255)
                                                                                                  aaTATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGtt                                           <  4:1066121/149‑1 (MQ=255)
                                                                                                    tatGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTa                                                                                <  1:683850/110‑1 (MQ=255)
                                                                                                    tatGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTa                                                                                >  2:683850/1‑110 (MQ=255)
                                                                                                       gCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCg                                      >  3:1138450/1‑149 (MQ=255)
                                                                                                           aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTa                                                                                <  1:538915/103‑1 (MQ=255)
                                                                                                           aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTa                                                                                >  2:538915/1‑103 (MQ=255)
                                                                                                             cAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAc                                                                                                                                 <  4:1093171/52‑1 (MQ=255)
                                                                                                             cAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAc                                                                                                                                 >  3:1093171/1‑52 (MQ=255)
                                                                                                                  tGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCa                                    >  4:731695/1‑140 (MQ=255)
                                                                                                                  tGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCa                                    <  3:731695/140‑1 (MQ=255)
                                                                                                                   gTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCaa                                                                                                                 >  1:2965/1‑61 (MQ=255)
                                                                                                                   gTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCaa                                                                                                                 <  2:2965/62‑2 (MQ=255)
                                                                                                                    tAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCg                              >  2:527661/1‑144 (MQ=255)
                                                                                                                    tAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCg                              <  1:527661/144‑1 (MQ=255)
                                                                                                                         gCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGc                    <  2:577478/149‑1 (MQ=255)
                                                                                                                              gAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCAt                                                                                                                 >  3:1146648/1‑51 (MQ=255)
                                                                                                                              gAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCAt                                                                                                                 <  4:1146648/51‑1 (MQ=255)
                                                                                                                              gAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGcc               >  3:477918/1‑149 (MQ=255)
                                                                                                                                 aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTg            >  2:960244/1‑149 (MQ=255)
                                                                                                                                     gaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTa                                                >  2:712601/1‑109 (MQ=255)
                                                                                                                                     gaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTa                                                <  1:712601/109‑1 (MQ=255)
                                                                                                                                     gaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAAc        >  1:51878/1‑149 (MQ=255)
                                                                                                                                       caaTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCATTGAAACAt      <  4:1261677/148‑1 (MQ=255)
                                                                                                                                           cAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAAc  <  1:960244/149‑1 (MQ=255)
                                                                                                                                            aGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATa     >  1:825133/1‑145 (MQ=255)
                                                                                                                                            aGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATa     <  2:825133/145‑1 (MQ=255)
                                                                                                                                               ttttAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCt                            <  3:283925/119‑1 (MQ=255)
                                                                                                                                               ttttAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCt                            >  4:283925/1‑119 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGTTATCCGCACTTTCGCTGACGTCGCCAGCCTTGAAACATAAAC  >  NC_000913/1880706‑1880993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: