Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,883,027	C→G	26.1%	intergenic (+30/‑161)	dmlA → / → yeaV	D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,883,027	0	C	G	26.1%	26.2 / 7.5	16	intergenic (+30/‑161)	dmlA/yeaV	D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase/putative transporter
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base G (2/2);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAT  >  NC_000913/1882885‑1883138                                                                                                                                               |                                                                                                                ttCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgc                                                                                                           >  2:711522/1‑149 (MQ=255)   cTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCgg                                                                                                         <  1:711522/149‑1 (MQ=255)           aaTTGAAGAAGTGATTGCTCCCGGGCCGAAAACAACTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGGAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAATGACGCGGTTTTTTTa                                                                                                 <  4:1390555/149‑1 (MQ=255)                     gTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCttt                                                                                       <  2:114232/149‑1 (MQ=255)                         ttGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgc                                                                                                           <  1:1374413/125‑1 (MQ=255)                         ttGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgc                                                                                                           >  2:1374413/1‑125 (MQ=255)                             tCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCa                                                                                    >  3:541494/1‑144 (MQ=255)                             tCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCa                                                                                    <  4:541494/144‑1 (MQ=255)                                       aaaaCACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCgg                                                                                                         <  1:1311050/113‑1 (MQ=255)                                       aaaaCACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCgg                                                                                                         >  2:1311050/1‑113 (MQ=255)                                                                                                                                     aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt  >  1:397275/1‑122 (MQ=255)                                                                                                                                     aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt  <  2:397275/122‑1 (MQ=255)                                                                                                                                         gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATg                                <  1:673246/88‑1 (MQ=255)                                                                                                                                         gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATg                                >  2:673246/1‑88 (MQ=255)                                                                                                                                         gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAt              >  1:376320/1‑106 (MQ=255)                                                                                                                                         gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAt              <  2:376320/106‑1 (MQ=255)                                                                                                                                               |                                                                                                                TTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAT  >  NC_000913/1882885‑1883138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑